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Biomedical subjects

Y Bai

Publications and source records attributed to Y Bai.

At least 181 records · Page 10Linked to original sources
PubMed1992

[Metabolism of serum lipoprotein in hypothyroidism].

The changes of serum lipids concentrations in 15 patients with primary hypothyroidism were studied. 9 euthyroid persons in the similar age group with patients were selected as a control group. The serum levels of total and LDL cholesterol, TG, ApoAI, ApoB and ApoCII in patients before treatment were all higher than those of the control group. They decreased to normal level after treatment. The mean values of total cholesterol decreased from 6.7 mmol/L to 4.1 mmol/L, TG decreased from 2.5 mmol/L to 1.0 mmol/L, LDI-ch from 3.9 mmol/L to 2.4 mmol/L, ApoAI from 1.9 g/L, to 1.3g/L, ApoB from 1.2 g/L to 0.7 g/L and ApoCII decreased from 6.9 mg/dl to 3.9 mg/dl. There were no differences of HDI-ch level between patients before treatment and normal controls, HDI-ch level of patients only slightly decreased after treatment. Soon after the thyroid hormone treatment, all parameters of serum lipids, not including HDI-ch, were improved, as TT, level became normal, although the levels of TT4 was still not reached to normal level.

Adult↗
PubMed1992

[Primary hypothyroidism with obstructive sleep apnea syndrome].

The relationship between severity of hypothyroidism and OSA syndrome before and after treatment was analyzed and discussed. All patients had typical clinical features of primary hypothyroidism and were diagnosed by thyroid hormonal investigations. They were treated with conventional desiccated thyroid. Polysomnography and blood oxygen saturation % (SaO2%) were performed during sleep before and after substitutive treatment. In this series of 21 patients taking respiratory functional tests, 11 were consistent with OSA (42.4%). The serum values of TT4, FT4I, TT3 and TSH after treatment were markedly different from the initial values in 9 cases who were followed up after treatment (P < 0.001, respectively). The respiratory parameters including maximum apnea time, mean apnea time, respiratory disturbances index and the lowest oxygen saturation % were also markedly different in patients before and after treatment (P < 0.001-0.02, respectively). The serum levels of TT4 and FT4I were significantly correlated with the values of maximum and mean apnea time and the highest oxygen saturation % after substitution (r = 0.64-0.98, P < 0.01-0.05, respectively). We conclude that OSA in hypothyroidism showed the most satisfactory response to non-surgical treatment, and OSA syndrome could be cured as soon as hypothyroidism was controlled by desiccated thyroid.

Adult↗
PubMed1991

Immunophenotyping of 515 cases of acute lymphoblastic leukemia in China.

Using cell surface markers and a panel of monoclonal antibodies, 515 cases of acute lymphoblastic leukemia (ALL) were immunophenotyped. T cell type ALL (T-ALL), non-T cell type ALL (Non-T-ALL) including common ALL (C-ALL), Null-ALL and B cell type ALL (B-ALL) were found. These major subtypes of ALL were further divided according to their phenotypes in detail. It was noticed that the phenotypes of these subtypes of ALL reflected basically the phenotypes of normal T or B cells at various differentiation stages or certain population of lymphocytes. The diagnosis of cell lineage was more precise when based on immunophenotyping than morphological description. The combination of morphological and immunological classification can improve the diagnosis of acute leukemias. In addition, it was observed that the immunophenotyping was relevant to clinicopathologic features, responses to therapy and prognosis of ALL patients. The incidences of major subtypes of ALL, the age distribution of ALL subsets and male sex bias with T-ALL in Chinese are discussed.

Adolescent↗
PubMed1991

[Correlation of immunophenotype with clinical features in T-cell acute lymphoblastic leukemia].

The immunophenotype of leukemicblasts from 111 patients with T-ALL or T-NHL were further examined by using a panel of standardized McAbs of CD nomenclature to human leukocyte differentiation antigens. Four major subsets of T-ALL were defined: pre T-ALL, immature T-ALL (I), common T-ALL (II) and mature T-ALL (III), with the percentages 20.7%, 20.7%, 20.7% and 37.0% respectively. In addition there was a case with M-T acute hybrid leukemia. Some of the clinical features of the patients with T-ALL and T-NHL were compared. It was found that male predominance, older age, higher leukocyte count, lower platelet level, relative higher hemoglobin level and increased incidence of extramedullary involvement, including hepatomegaly, splenomegaly and lymphadenopathy were alike for all subsets of T-ALL cases. However, the average white cell level and incidence of lymphadenopathy in the pre T-ALL subset significantly differed from those in other subsets. The correlation of immunophenotype with morphologic characterization was also discussed in this paper.

Adolescent↗
PubMed1990

Characterization of cDNA encoding a human sperm membrane protein related to A4 amyloid protein.

A rat testis lambda gt11 cDNA library was screened with a monoclonal antibody raised against a human sperm membrane protein designated YWK-II. A clone was found with a cDNA insert composed of 1837 base pairs that contained an open reading frame coding for 191 amino acid residues. The deduced polypeptide contained a segment with high homology to the transmembrane-cytoplasmic domains of the A4 amyloid protein found in brain plaques of Alzheimer disease patients. A sequence of basic amino acid residues, Arg-Lys-Arg, was found instead of Lys-Lys-Lys at the probable membrane-cytoplasmic junction that may be a unique property of sperm membrane proteins.

Amino Acid Sequence↗
PubMed1990

Monoclonal antibody 55 (CD10) and complement used for purging autologous bone marrow in common acute lymphoblastic leukemia.

A CD10 monoclonal antibody 55 (McAb55) was intended for purging residual common acute lymphoblastic leukemia antigen (CALLA) positive leukemic cells from autotransplants of common acute lymphoblastic leukemia (C-ALL) patients. It was found that after two rounds of McAb55 and complement treatment, 4-5 logs of CALLA+ cells were removed from bone marrow detected by clonogenic assay. The standardization of separation, purgation and preservation of bone marrow for C-ALL patients' autotransplants was then set as follows: Following the carboxymethyl starch sedimentation and Ficoll-Hypaque gradient separation, the isolated mononuclear cells (MNCs) were treated with McAb55 and complement twice and kept in room temperature for 48-72 hours prior to infusion. This procedure resulted in the removal of more than 99% of CALLA+ cells, recovery of 10-30% MNCs, and leaving the hematopoiesis stem cells intact. After the intensive cytoreductive therapy, 4 patients with C-ALL received the purged autotransplants giving timely recovery of the hematopoietic function. The patients were all remaining in remission status for more than 40-250 days so far.

Adult↗
PubMed1990

[Neuropathy and myopathy in patients with chronic lymphocytic thyroiditis].

Neurological investigations were performed for 53 cases with chronic lymphocytic thyroiditis diagnosed by pathology and serum antithyroid antibodies determination. Of the 53 cases, 29 had different types and severity with neurological findings, which could be divided into 5 groups: 1) Elevated levels of acetylcholine receptor antibody in 13 cases (24.1%); 2) Myopathy and weakness accompanied by elevated serum enzyme levels (GOT, CPK, LDH and alpha-HBDH) in 7 cases (13%); 3) Peripheral neuropathy in 6 cases (11.1%); 4) Encephalopathy in 2 cases (3.7%); and 5) The changes in sella turcica in 2 cases. Our data showed that the neuropathy was not closely correlated to the duration of chronic lymphocytic thyroiditis and seemed that it had no relation with the thyroid function and titer of antithyroid antibodies. However, neuropathy occurred more often in cases with both chronic lymphocytic thyroiditis and some other autoimmune disorders, suggesting that abnormal immune function might be the common background patients with chronic lymphocytic thyroiditis and neuropathy. The aforementioned data suggest that the hypothyroid function and high titer of antithyroid antibodies might not be a prerequisite for developing neuromyopathies.

Adolescent↗
PubMed1990

Effect of acute water loading on plasma levels of antidiuretic hormone AVP aldosterone, ANP fractional excretion of sodium and plasma and urine osmolalities in myxedema.

We monitored the plasma and urine osmolalities, fractional excretion of sodium, fractional excretion of chloride, plasma levels of antidiuretic hormone (ADH, AVP), aldosterone and atrial natriuretic peptide (ANP) before and after acute water ingestion in 12 patients with overt hypothyroidism. The ability of the patients to dilute and concentrate urine was found impaired and the ability of excretion of water load decreased and delayed. Acute water load test was proved to be effective in evaluating the urinary excreting function for the patients. We hypothesize that inappropriate secretion of anti-diuretic hormone and elevated plasma ANP may be homeostatic factors for abnormal urinary excretion in patients with hypothyroidism.

Adolescent↗
PubMed1990

Cloning and identification of a rabbit sperm membrane protein gene.

Antibodies raised against rabbit sperm membrane protein (rSMP-B) interacted with a rat testis cytosolic protein with an estimated molecular weight of 70 kD. The clone expressing rSMP-B was isolated by epitope selection from a rat testis lambda gt11 vector cDNA library. An Eco R1-cut 2.0 kb cDNA fragment encoding rSMP-B was identified. The cDNA fragment (RSD-1) was isolated, subcloned and restriction mapped. Dot hybridization analysis of mRNA prepared from human, rabbit and rat testes showed that the gene encoding rSMP-B is expressed in the testes of all three mammals.

Animals↗
PubMed1990

[Study on urinary function and metabolism of water and electrolytes in primary hypothyroidism].

Water excretion in 16 patients with primary hypothyroidism was lower in amount and more delayed (P less than 0.05) than that in 5 normal controls after acute water ingestion. The mean plasma osmolality of the patients was lower than that of normal controls both before and after water loading. However, the mean urine osmolality was not decreased but rather elevated. The clearance of free water in the patients was lower than that in normal controls before loading, Although the clearance of osmolality in the patients was higher than that in the controls, the difference was insignificant. These two clearance rates were lower than those in the controls after loading. The fractional excretion of sodium (P less than 0.05) and chloride (P less than 0.01) in the patients was significantly higher than that in the controls before loading and both of them remained elevated after loading. Most of the parameters mentioned above improved in 9 patients after treatment with desiccated thyroid.

Adolescent↗
PubMed1990

[Effect of dry ginger and roasted ginger on experimental gastric ulcers in rats].

This paper reports the effect of dry ginger and roasted ginger decoctions on four experimental gastric ulcer models in rats at an oral administration of 4.5 g/kg. The result shows that the roasted ginger has an obvious inhibiting tendency on three gastric ulcer models except the indomethacin induced model while the dry ginger has no such effects. The acute toxicity test has shown that the LD50 of roasted ginger decoction administered orally is 170.6 +/- 1.1 g/kg, but it is over 250 g/kg with dry ginger. This suggests that the water soluble constituents of the dry ginger have changed in the roasting process.

Acetates↗
PubMed1990

[Determination of traces of gallium in air by oscillographic polarography].

Gallium and its compounds in air collected by millipore filter membrane are leached by 10% HNO3 at room temperature. After being adjusted with ammonia solution to pH8-9, the leachate is filtered and the filtrate is evaporated to dryness on a water bath. The residue is dissolved with 2 ml of 10% HNO3 and then into the solution are added 0.5 ml of 5% hydroxylamine hydrochloride solution, 0.20 ml of 5% sulfosalicyclic acid solution, 0.5 ml of NaF solution (1mg F/ml) and 1 drop of methyl red solution. Let it stand for 5 min after the color of the solution turns orange by addition of 1:1 NH4OH solution. Add 0.20ml of 0.20% cupferron solution, 0.20ml of 0.5% diphenyl-guanidine solution (prepared in 95% ethanol) and 0.5ml of acetic acid-ammonium acetate buffer (pH 7.0). Then add water to make the final volume of the test solution to be 10ml and measure the height of the second derivative polarographic wave at -1.08V (vs.SCE). The sensitivity of the method is 0.005-0.10 microgram/ml, the coefficient of variation 6.8%, recovery 97.8%. Within 0.005-0.10 microgram/ml, the wave height is linear with the concentration of gallium.

Air↗
PubMed1989

Sequence-dependent reactivity of model peptides with glyceraldehyde.

Glyceraldehyde reacted faster with tripeptides than with dipeptides. The pH profiles of the reactions with tripeptides displayed optima in the range of 8.5-10.0, approximately 1-2 pH units higher than found with dipeptides. The second amino acid residue influences not only the rate of reaction but also the extent of formation of the product of the Amadori rearrangement, the ketoamine. The presence of histidine as the second amino acid residue of either di- or tri-peptides greatly accelerated the rate of reaction perhaps by facilitating the rearrangement. Conventional amino acid analysis and liquid chromatography procedures have been used to detect intermediates and the ketoamine product. 1H-N.m.r. analysis of the reduced adducts was consistent with the assigned structures.

Amino Acid Sequence↗
PubMed1989

Some factors that influence the nonenzymatic glycation of peptides and polypeptides by glyceraldehyde.

The rate of reaction of glyceraldehyde with a series of peptides was found to be dependent on their amino acid composition, sequence, and chain length. The presence of a histidine near the NH2-terminal increased the rate of glycation, whereas the presence of a carboxyl group near the reaction site led to a decrease in reaction rate. In general, tripeptides reacted faster than dipeptides, and dipeptides reacted faster than amino acids. Sodium phosphate and 2,3-diphosphoglycerate enhanced the rate of reaction of glyceraldehyde with all the dipeptides tested. Sodium chloride inhibited the reaction in phosphate buffer, but not in HEPES buffer. The NH2-terminal heptapeptide from the beta-chain of human hemoglobin A (HbA), where histidine is the second residue, reacted with glyceraldehyde faster than the NH2-terminal hexapeptide from the alpha-chain. The glycation of tetrameric human Hb by glyceraldehyde was found to be dependent on the ligation state of the protein since deoxy-HbA reacted about 50% more with glyceraldehyde than did liganded HbA. The enhanced glycation of deoxy HbA was mainly attributable to the more extensive reaction at the NH2-terminal of the beta-chain. The presence of a histidine adjacent to the NH2-terminal at this site may facilitate the Amadori rearrangement. The glycation of horse Hb in which the second residue is glutamine was not increased under anaerobic conditions.

Amino Acids↗