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Biomedical subjects

Y Amano

Publications and source records attributed to Y Amano.

At least 163 records · Page 9Linked to original sources
PubMed1985

Stimulation and inhibition of DNA synthesis and cell growth by paraquat in cultured cells.

Paraquat, a popular herbicide, is known to be very toxic to man and animals. Using cultured mammalian cells (human embryo lung cell, R-66), the effect of paraquat has been studied. With autoradiography, stimulation of DNA synthesis was observed when the cells were exposed to 0.08 or 0.4 microM of paraquat; however, DNA synthesis was inhibited with 40 microM of paraquat. To determine cell growth rate, accumulation of mitotic cells was determined after addition of colcemid (0.2 microgram/ml) into the culture medium. The ratios of mitotic cells observed were proportional to the level of DNA synthesis. And the stimulation and inhibition of cell growth were observed even after washing off paraquat from media.

Autoradiography↗
PubMed1985

The mechanisms of systemic symptoms following nasal administration of allergens.

A nasal provocation test was performed by consecutively placing allergen discs on the nasal mucosa of patients with pollinosis. This brought easily the systemic symptoms such as cough, periorbital oedema, and urticaria. Similarly, the nasal administration of large doses of the allergen induced a change in the respiratory response in sensitized guinea pigs. These results indicate that nasal mucosa absorbs a high molecular substance such as allergens.

Administration, Intranasal↗
PubMed1984

A direct evidence for defect in glucose-6-phosphate transport system in hepatic microsomal membrane of glycogen storage disease type IB.

Uptake of glucose-6-phosphate by microsomes of hepatocyte in rats, human controls and patients with glycogen storage disease type Ia and Ib was studied. In rat the uptake of glucose-6-phosphate increased rapidly and reached to a plateau, but mannose-6-phosphate was not accumulated. These findings indicate that a glucose-6-phosphate specific transport system exists in the microsomal membrane. In human controls and patients with glycogen storage disease type Ia the uptake of glucose-6-phosphate was clearly observed. On the other hand, no accumulation of it was detected in a patient with glycogen storage disease type Ib. These data provide a direct evidence of the defect in the glucose-6-phosphate transport system of hepatic microsomal membrane in glycogen storage disease type Ib.

Animals↗