Search PubMed⌕ Search

Biomedical subjects

Xing-hua Huang

Publications and source records attributed to Xing-hua Huang.

10 recordsLinked to original sources
PubMed2006

[Fluid-based thin-layer method for screening of squamous intraepithelial lesions in pregnant women].

OBJECTIVE: To investigate the clinical significance and safety of liquid-based cytology (thinprep cytologic test, TCT) in pregnant women and cytological characteristics of the smears during pregnancy. METHODS: TCT were performed in 1951 pregnant women (group A), 3752 gynecological out-patients (group B) and 5069 routine screening women (group C). The age of the women was ranged from 20 to 39 years. The cytological preparations were analyzed using TCT method following the Bathesda system (TBS) standard. RESULTS: (1) The incidence of abnormal epithelial patterns in groups A, B and C was 23.58% (460/1951), 19.22% (721/3752) and 13.40% (679/5069) respectively (P < 0.01). (2) The incidence of infection in three groups was 19.02% (371/1951), 10.66% (400/3752) and 9.23% (468/5069) respectively (P < 0.05). (3) The incidence of cervical intraepithelial neoplasia (CIN) in three groups was 1.13%, 1.87% and 0.77% respectively. No significant difference was found between pregnant group (A) and non-pregnant group (B + C, P > 0.05). CONCLUSIONS: TCT smear screening is necessary, safe and acceptable for routine prenatal tests. There is no difference in the cervical squamous intraepithelial lesions between pregnant and non-pregnant women.

Adult↗
PubMed2004

Effect of propentofylline on hypoxic-ischaemic brain damage in newborn rat.

BACKGROUND: Studies showed that propentofylline enhances the action of adenosine and protects hippocampal neuronal damage against transient global cerebral ischaemia. Our study was to investigate the effect of propentofylline on hypoxic-ischaemic brain damage in neonatal rat. METHODS: Seven-day-old Wistar rats were subjected to unilateral common carotid artery ligation and hypoxia in oxygen 8 kPa for two hours at 37 degrees C. Propentofylline (10 mg/kg) was administered intraperitoneally one hour after hypoxia-ischaemia (treated group). Control group rats were received an equivalent volume of saline. The effects of propentofylline were assessed by observing the body mass gain, behavioural alteration and neurohistological changes. The rats were sacrificed at 72 hours after hypoxia-ischaemia, and the brain sections were examined after haematoxylin and eosin staining. RESULTS: The propentofylline-treated rats had better body mass gain and better behavioural response than the paired saline-controls did. In the control group, the rats either lost body mass or had little mass gain after the insult, their average body mass gain was 97.3% at 24 h, 100.3% at 48 h, and 114.1% at 72 h of recovery. In propentofylline-treated group, there was a significant improvement of body mass gain at 24 h (100.2%, P < 0.05) and 48 h (110.3%, P < 0.01) of recovery; the percentage of rats that performed well on behavioural test was significantly higher from 48 h to 72 h of recovery (P < 0.05); the incidence of severe brain damage to the cerebral cortex and dentate gyrus was significantly reduced in propentofylline-treated rats (cortex, 93% - 70.8%, P < 0.01; dentate gyrus 95% - 66.7%, P < 0.01) as compared with control rats. CONCLUSIONS: Administration of propentofylline 1 hour after hypoxia-ischaemia significantly attenuates brain damage in both the cerebral cortex and dentate gyrus, and also improves the body mass gain as well as behavioural disturbance in 7-day-old rats.

Animals↗
PubMed2003

[Multicenter study on screen method for gestational diabetes].

OBJECTIVE: To investigate the feasibility of using random blood glucose to screen gestational diabetes mellitus (GDM). METHOD: The random blood glucose was determined in 1 038 pregnant women between 24 and 32 gestational weeks. Then 50 gram glucose challenge test (50 g GCT) was performed followed immediately. Finally, 75 gram oral glucose tolerance test (75 g OGTT) was done without dietary control for 3 days. If two values of four were abnormal, GDM was diagnosed. Impaired glucose tolerance (IGT) was diagnosed if only one value was abnormal or the 2nd hour value ranged from 120 to 164 mg/dl. RESULTS: (1) The determination of the three steps was completed in 948 cases. Among them, 42 cases (4.4%) were GDM, 372 cases (39.2%) were IGT and other 534 cases were normal. (2) In the normal group, the random blood glucose were different in fasting and postprandial times. No difference was found among blood glucose values determined of 50 g GCT at different times except that the value of 50 g GCT 1 hour postprandial was higher than the value at other times. There was no significant association between random blood glucose and 50 g GCT. (3) The sensitivity and specificity were 50.0% and 67.7%, when IGT was diagnosed using the cut point of 6.4 mmol/L (115 mg/dl) of random blood glucose, which was similar with 51.1% of sensitivity and 71.2% of specificity when using >or= 7.8 mmoL/L (140 mg/dl) as the cut point of 50 g GCT. If 6.4 mmol/L (115 mg/dl) was used as the cut point in GDM group the sensitivity would be 80.0%, which was much higher than that of IGT group and the specificity was 61.2%. In this study, if the value of >or= 8.3 mmoL/L (150 mg/dl) was used as the cut-point of 50 g GCT to screen the GDM, the sensitivity decreased only 2.0% while the specificity increased more than 10.0%. CONCLUSIONS: (1) The determination of random blood glucose to screen GDM couldn't replace the 50 g GCT, but it can be used as a complemental method and can be used repeatedly at any gestational age and convenience the pregnant women and the doctors. (2) The value of 8.3 mmol/L (150 mg/dl) was used as the cut-point of 50 g GCT, the specificity would be increased and the requirement for OGTT would be lowered markedly, which would reduce economic and psychological stress.

Adult↗
PubMed2003

[Postpartum reclassification in women with gestational diabetes and analyzing the high risk factors associated with them].

OBJECTIVE: To follow up the 75 g oral glucose tolerance test (OGTT) in gestational diabetes mellitus (GDM) at 2 month after birth for reclassifying and analyzing the risk factors associated with the abnormal blood glucose level. METHODS: 294 cases of GDM underwent a 2 hours 75 g OGTT two months postpartum. According to the WHO diagnostic criteria, they were divided into three groups: (1) type 2 diabetic mellitus (DM), impaired glucose tolerance (IGT) and normal. The related factors during pregnancy were analysed. RESULTS: 160 cases (54.4%) were normal, 75 cases (25.5%) and 59 cases (20.1%) were IGT and DM respectively. (2) In the DM group, the gestational age at diagnosis was much earlier than those of the other two groups (P < 0.01), the glucose level of 50 gram glucose challenge test (GCT) and fasting blood glucose values in OGTT and the value of HbAlc at diagnosis were evidently higher than the other two groups (P < 0.01, P < 0.01, P < 0.01), the gestational age of use of insulin for treatment was earlier than normal group, the dosage used markedly greater than the other two groups, the fasting blood glucose and 2 hours postprandial glucose were significantly higher than other two groups (P < 0.01) within 7 days after birth. Maternal age, body weight and family history did not show difference in the three groups. CONCLUSION: Among the GDM, about 1/4 were IGT and 1/5 were type 2 DM. The DM group showed early diagnosis, high fasting blood glucose, high frequency to use insulin during pregnancy.

Adult↗
PubMed2003

[The blood lead level and pregnant outcome in pregnant women with non-occupational lead exposure].

OBJECTIVE: The aim of this study is to find out the relations between maternal blood lead and pregnancy complications, neonatal physical and neurobehavioral development respectively. METHODS: 1,151 samples of maternal venous blood and 434 samples of umbilical cord blood were collected. Graphite stove atomic absorption spectrophotometer was used to detect the lead concentration. Neonatal physical indexes were measured within 24 hours of age, including weight, length, head circumference, chest circumference and abdomen circumference. The neurobehavioral developments were scored at 42 days of age. Meanwhile, the pregnancy complications were obtained from the medical records. RESULTS: (1) the mean lead concentration of maternal blood is 43.4 microg/L while 44.5 microg/L of first trimester, 37.4 microg/L of second trimester and 45.1 microg/L of third trimester. The mean lead concentration of cord blood is 26.4 microg/L. (2) In this study, the blood lead level is not correlated with pregnancy complications and neonatal physical and neurobehavioral development, but it is positively correlated with maternal haemoglobin concentration. CONCLUSIONS: In this study, the situation of maternal blood lead level is acceptable. At such level, the lead concentration of pregnant women blood can't influence the incidence of pregnancy complications, neonatal physical and neurobehavioral development.

Adult↗
PubMed2003

[Study on intrapartum service model during normal labor].

OBJECTIVE: To study the availability of humanized service and healthy birth and it's effect on maternal and neonatal outcomes. METHODS: A multicenteral prospective randomized control study was carried out in 9 maternity hospitals from Nov. 2000 to June 2001. 6,758 laboring women were randomly divided into study group and control group. In study group (n = 3,437), midwives accompany laboring women in whole course of birth until two hours after delivery. According WHO "Monitoring rules for normal birth" medical staff support the mothers in physiological, psychological and physical aspects. In control group (n = 3,321) mothers receive traditional management. Midwives visit and check the mothers discoutinually. In two groups husbands can company laboring women according women's requirement. A questionnaire will be filled on the second day after birth. RESULTS: In study group the spontaneous delivery rate was significant higher than that of control group (85.5% vs 66.0%, P < 0.01). The cesarean section rate, postpartum hemorrhage rate, neonatal asphyxia rate and neonatal morbidity were marked lower in study group than those of control group (P < 0.01 and P < 0.05). CONCLUSIONS: Appropriate mode of intrapartum care can decrease the cesarean section rate and promote the vaginal delivery, also it can improve the maternal and neonatal outcomes.

Delivery, Obstetric↗
PubMed2003

[Mutation analysis of fibroblast growth factor receptor 3 gene in an achondroplasia family].

OBJECTIVE: To clarify the patients' pathogenic mechanism in an achondroplasia family not according with the genetic law of autosomal dominant inheritance disease at gene level. METHODS: Genomic DNA from peripheral blood of all members in this family was used for amplification of the exon 10 of fibroblast growth factor receptor 3(FGFR3) gene by PCR; mutation was detected by DNA sequencing and identified by restriction endonuclease MaeIII. RESULTS: A new mutation of A to T at nucleotide 1180 was found in patients but not in unaffected members. CONCLUSION: Combined with pedigree analysis, it was summarized that achondroplasia patients in this family might result from this new mutation.

Achondroplasia↗
PubMed2003

[Study on diagnostic criterion of pregnancy induced hypertension].

OBJECTIVE: To study the rationality of the current diagnostic criterion of pregnancy induced hypertension (PIH) in mainland China. METHODS: To analytically review the 916 cases of PIH in our hospital, reclassify them using both the current domestic diagnostic criteria and the William Obstetrics' criteria, then compare and analyze perinatal and maternal outcomes from the two standards. RESULTS: (1) Maternal outcomes: comparison result of the occurrences among three groups of premature birth, postpartum haemorrhage and placental abruption according to domestic classification had significant difference (P < 0.05). And it's the same result according to foreign classification (P < 0.05). But the occurrences within three groups of premature birth, postpartum haemorrhage and placental abruption between domestic and foreign classifications showed no significant difference (P > 0.05). (2) Perinatal outcomes: Comparison results of the occurrences among three groups of fetal growth restriction, asphyxia neonatorum and perinatal fetal and neonatal death incidence according to domestic classification had significant difference (P < 0.05). And it's the same result according to foreign classification (P < 0.05). But the occurrences within three groups of fetal growth restriction, asphyxia neonatorum and perinatal fetal death and neonatal incidence showed no significant difference (P > 0.05). (3) The constituent ratio of mild, moderate, severe PIH according to domestic classifications was 46.6%, 18.4%, 34.9% respectively. The constituent ratio of gestationgal hypertision, mild preeclampsia and severe preeclampsia according to foreign classifications was 47.6%, 14.3%, 38.1%. There were significant differences between domestic and foreign constituent ratio (P < 0.05). If you compare the results from these two diagnostic classifications, with mild PIH, the accordant rate was 85.0%; with severe PIH, the rate was 93.8%; but it's only 40.2% with moderate PIH. CONCLUSIONS: The diagnostic criteria for PIH being used in our mainland China is rational, but in the current stage, due to the lack of objective index for early diagnosis and overall assessment of patient's condition, it's also limited. More objective index should be adopted to improve the diagnostic standard for PIH.

Female↗
PubMed2002

[Clinical observation on treatment of postpartum hemorrhage by xuesaitong soft capsule].

OBJECTIVE: To observe the effect of Xuesaitong (XST) soft capsule in treating postpartum hemorrhage. METHODS: Sixty pregnant women were randomly divided into the treated group (A) and the control group (B), 30 cases in each, they were treated with XST and placebo respectively for 7 days to observe the changes of hemorrhagic conditions. RESULTS: After delivery, the hemoglobin, RBC count, hematocrit and cardiac index were lower at 3rd day than those before delivery in both groups (P < 0.05 or P < 0.01), reticulocyte count lowered insignificantly as compared with that before delivery in group A but significantly in control group B; the bleeding time shortened significantly in group A (P < 0.05) but unchanged in group B; significant change of blood viscosity only found in group B (P < 0.05). The amount of bleeding 30 min, 1 hr, 2 hrs and 24 hrs after delivery in group A was all lower than that in group B respectively (P < 0.01 or P < 0.05). Involution of uterus in group A at 2nd, 4th and 7th day after delivery was all better than that in group B respectively (P < 0.05). CONCLUSION: XST has definite effect in decreasing postpartum hemorrhage, it also shows effects in promoting involution of uterus, reducing lochia and accelerating recovery of parturients.

Adult↗