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Biomedical subjects

Xiao Ma

Publications and source records attributed to Xiao Ma.

2 recordsLinked to original sources

Complete mitochondrial genomes and phylogenetic analysis of three species of Indo-Pacific freshwater gobies, Stiphodon (Gobiiformes,Oxudercidae).

Stiphodon is a genus of gobioid fishes found primarily in freshwater streams on islands throughout the Indo-Pacific region. It is the most speciose genus of Sicydiinae, with more than three dozen named species. Complete mitochondrial genome (mtDNA) sequences were determined for three species, namely Stapledon atropurpureus, Stapledon elegans, and Stapledon semoni. Maximum likelihood and Bayes inference inferences from 13 protein-coding genes (11,433 bp) or the COXI gene (1,551 bp) alone were made on these three together with four mitogenomes previously available. S. percnopterygionus and S. tuivi formed an outgroup to the other five Stiphodon species, meanwhile Stapledon atropurpureus and S. semoni were recovered as sister species. The ratios of the non-synonymous to the synonymous ubstitution rates for all PCGs were in the range 0\ (Ka/Ks) \1, which suggests they have been subject to purifying selection.

Animals

EZH2 variants derived from cryptic splice sites govern distinct epigenetic patterns during embryonic development.

EZH2 catalyzes H3K27me3 and is essential for embryonic development. Although multiple EZH2 variants have been identified, the functional implications and physiological significance of its heterogeneity remain unclear. Here, we revealed that conserved cryptic splice sites generated two EZH2 variants with (EZH2A) or without (EZH2B) a 27-nt region, coding for a 9-aa segment. Structural modeling showed that splice-in or splice-off of the 9-aa segment caused a topological change in EZH2 structure. The 9-aa surplus in EZH2A strengthened its interaction with other PRC2 components, particularly in PRC2.2 holocomplex. We developed point-mutation mouse lines specifically depleting EZH2A or EZH2B (Ezh2amut or Ezh2bmut). Biallelic deletion of Ezh2a caused developmental defects and embryonic lethality between E12.5 and E15.5, while the Ezh2bmut mice were fertile and developed normally. Combined RNA-seq and CUT&Tag analyses in mouse embryonic fibroblasts revealed that EZH2A and EZH2B bound to different genomic loci and affected H3K27me3 deposition in different subsets of genes related to development or the innate immune system, respectively. EZH2A depletion specifically suppressed the expression of genes involved in the development-related Hippo-Yap1 pathway, which might be attributable to a compensatory process mediated by JARID2. Our findings demonstrate that EZH2 heterogeneity from the 9-aa splicing event plays a crucial role in development.

Animals