Biomedical subjects

Xiang-Fu Wu

Publications and source records attributed to Xiang-Fu Wu.

77 records · Page 5Linked to original sources

PubMed1997

Studies on the Expression of the 28 kD Glutathione S-transferase Gene from Schistosoma japonicum in the Silkworm (Bombyx mori) Larvae and Insect Cells.

The gene of the 28 kD glutathione S-transferase (GST) from the Chinese strain of Schistosoma japonicum had been expressed in the silkworm (Bombyx mori) cells and larvae by Bombyx mori nuclear polyhedrosis virus (BmNPV) vector which had been modified. The GST gene was inserted into the right position of the BmNPV genome as identified by Southern hybridization. The product was a 28 kD protein, had GST activity and antigenicity. The yield in BmN cells (1x10(6) cells/ml) was 0.77 mg and more than 5 mg in a silkworm larvae. All the results showed that it is possible to develop the GST expressed in the silkworm larvae to a new kind of genetic engineered schistosomiasis vaccine.

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PubMed1996

Cloning of the BPLA(2) Gene from Agkistrodon halys Pallas.

First strand cDNA synthesis was primed with synthetic oligonucleotide from total RNA extracted from the gland of the snake Agkistrodon halys Pallas. The pro-BPLA(2) gene was then amplified by PCR and cloned into the pBS-ks vector. Its nucleotide sequence has been determined by analyzing the DNA sequence of three colonies containing the pro-BPLA(2) gene. The deduced amino acid sequence consists of 138 amino acids and agrees with the partly known amino acid sequence except for seven amino acid residues. The successful cloning of the BPLA(2) gene not only has made the determination of its total amino acid sequence possible, but also provided a good basis for further research work in the protein engineering of functional peptides from snakes.

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PubMed1996

The Recombination and Expression of a Chimeric Antibody Light Chain Gene in Cells of Silkworm Bombyx mori.

A chimeric immunoglobulin light chain gene had been expressed in a baculovirus expression system, which was achieved by infecting the silkworm cells with the recombinant virus r-NPVL2 containing the cDNA of immunoglobulin against a small cell lung cancer. PCR analysis and Southern hybridization proved that the antibody light chain cDNA hand been integrated into the modified BmNPV genome. Expression products of human-mouse chimeric antibody light chain in the silkworm cells were detected by both Western blot and ELISA.

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PubMed1996

Expression of Human Bone Morphogenetic Protein 2 in Bombyx mori Larvae and Its Purification.

Recombinant Bm-BacPAK-BMP2 virus which contains the human bone morphogenetic protein 2 (BMP2) cDNA gene under the control of polyhedrin gene promoter was generated by cotransfection into Bm-N cells with the transfer plasmid pBacPAK-BMP2 and the modified Bombyx mori nuclear polyhedrosis virus Bm-BacPAK genomic DNA. On the fifth day after infection with the recombinant virus, Bombyx mori larvae produced about 10 &mgr;g rBMP2 in 1 ml hemolymph. The expressed rBMP2 was processed into 16 kD C-terminal fragments and formed 30 kD dimmers with disulfide bonds in vivo. The protein was purified to about 90% purity and induced cartilage locally in rats 7 days after subcutaneous transplantation.

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PubMed1996

The Cloning and Construction of a Murine-human Chimeric Antibody with Specificity for HBsAg and the Expression of the Chimeric Heavy Chain in E. coli.

The genes encoding the heavy and light chain variable regions (V(H) and V(K)) of the murine monoclonal antibody with the specificity for hepatitis B virus surface antigen (HBsAg) have been clone by using RT-PCR. They were combined to the Human C(gamma)(3) and C(K) genes respectively to construct the murine human chimeric antibody genes The chimeric heavy chain was expressed in E. coli, and characterized by SDS-PAGE, Western-blot, indirect ELISA Assay to demonstrate the expression product has the HBsAg-binding ability.

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