Biomedical subjects

X Zhu

Publications and source records attributed to X Zhu.

At least 163 records · Page 9Linked to original sources

PubMed2001

[Role of costimulating molecule CD86 in maternal-fetal immune regulation].

OBJECTIVE: To evaluate the role of costimulating molecule CD86 in maternal-fetal immune regulation. METHODS: In abortion-prone (CBA x DBA/2) matings and non-abortion-prone (CBA x BALB/c) matings, the pregnant CBA females were injected intraperitoneally by purified rat anti-murine CD86 monoclonal antibody (mAb) with dosage of 100 micrograms at day 4, 6, 8 and 10 of gestation respectively, or injected only once at day 4 of gestation, or injected with purified rat IgG as control. The resorbing and non-resorbing embryos were enumerated at day 14 of gestation. RESULTS: The embryo resorbing rate in CBA x DBA/2 matings was 27.78%, and that in CBA x BALB/c matings was 8.42%. After injection of anti-CD86 mAb at day 4, 6, 8, 10 of gestation, the embryo resorbing rate reduced to 9.68% (P < 0.005) in CBA x DBA/2 matings, while that was 13.54% in CBA x BALB/c matings (P > 0.05). After injection of anti-CD86 mAb only once at day 4 of gestation, the embryo resorbing rate reduced apparently to 7.14% (P < 0.001) in CBA x DBA/2 matings, while that was 11.39% (P > 0.05) in CBA x BALB/c matings. CONCLUSIONS: Blocking the costimulating signal by anti-CD86 mAb at maternal-fetal interface, especially at the prime stage of maternal-fetal recognition (periods of implantation), may result in maternal-fetal tolerance, and hopefully the embryo resorption turns to normal pregnancy.

Animals↗

PubMed2001

Prevention and treatment of hepatitis B relapse after liver transplantation.

OBJECTIVES: To investigate the effect of orthotopic liver transplantation (OLT) on hepatitis B(HB)-related diseases and the efficiency of prevention and treatment with lamivudine on recurrence of hepatitis B posttransplant in China. METHODS: Orthotopic liver transplantation (OLT) under veno-venous bypass was performed in 10, of whom 9 males had hepatitis B and 1 female had hepatocellular cancer (HCC) without HB pretransplant. Eight of the 9 males had fulminant hepatitis B (FHB) and they all had preoperative serious jaundice, ascites and coagulopathy. Six had encephalopathy; 1 was associated with acute renal failure, and 1 with gastrointestinal hemorrhage. Seven of the 10 patients had lamivudine to prevent reinfection with hepatitis B. RESULTS: Of the 8 patients who have survived for 2-12 months, 7 have survived for 6-12 months. Two died, one of recurrent FHB and the other from multi-organ failure (MOF). Seven preoprative HB patients of the 8 survivors have excellent liver function through 1 has positive HBsAg 6 months after OLT. One of the 8 survivors, the female with HCC pretransplant, suffered hepatitis B 6 months after OLT and her hepatic function has been gradually improving with lamivudine therapy. CONCLUSIONS: OLT is an effective therapy for certain cases of FHB and HCC and lamivudine may prevent recurrence of hepatitis B after OLT.

Adult↗

PubMed2001

Hepatic angiomyolipoma: a clinicopathologic study of 10 cases.

OBJECTIVE: To study the clinicopathologic features of hepatic angiomyolipoma (AML) and to investigate the feasibility of a new antibody-A103 as a diagnostic aid for AML. METHODS: Ten cases of AML were retrieved from hospital records and analyzed morphologically. Immunohistochemistry was performed on paraffin-embedded tissues with a panel of antibodies, including antibody-A103. RESULTS: There were eight women and two men, with ages ranging from 38-58 years (median 45.7). Clinically, nine cases were asymptomatic and found by imaging techniques. None of the patients had associated tuberous sclerosis. All tumors were sharply demarcated from the surrounding liver parenchyma. Histologically they were composed of a heterogeneous mixture of three components: thick-walled blood vessels, spindle or epithelioid smooth muscle cells and adipose tissue. All tumors showed a strong immunoreactivity to A103, HMB-45 and smooth muscle actins. Follow-up information on all 10 cases showed a benign course with no signs of recurrence. CONCLUSIONS: Hepatic AML is a rare mesenchymal tumor of the liver. A103 is a promising marker for a pathologic diagnosis of hepatic AML.

Actins↗

PubMed2001

Evaluation of coronary artery bypass graft patency using three-dimensional reconstruction and flow study of electron beam tomography.

OBJECTIVE: To establish and evaluate two protocols for the noninvasive visualization and assessment of coronary artery bypass graft (CABG) patency on electron beam tomography (EBT). METHODS: Two hundred and fourteen consecutive patients who underwent coronary artery bypass graft surgery were scanned using both EBT angiography with 3-dimensional reconstruction and EBT flow study with time-density-curve analysis. RESULTS: There were 589 CABGs evaluated in this study (10 grafts were excluded because of artifacts). Among them, 133 (98.5%) of 135 arterial grafts were patent, and 345 (77.7%) of 444 saphenous-vein grafts were patent. Within 5 years or between 5 and 10 years after operation, arterial graft patency exceeded venous graft patency (P < 0.001). Three-dimensional EBT angiography achieved higher sensitivity, specificity and accuracy (97.7%, 94.1% and 96.7%, respectively) than did EBT flow study (88.4%, 82.4% and 85.2%, respectively) for evaluating occlusion or patency of CABG. The intra-graft flow of patent arterial and venous grafts were 4.9 +/- 2.2 ml.min-1.g-1 and 6.9 +/- 2.8 ml.min-1.g-1, respectively (P < 0.001). CONCLUSION: The combination of EBT three-dimensional reconstruction and flow study can be more effective in the assessment of CABG anatomy and quantification of patent CABG blood flow.

Adult↗

PubMed2001

Retroviral vector containing human p16 gene and its inhibitory effect on Bcap-37 breast cancer cells.

OBJECTIVE: To study the effects of the p16 gene on tumor cell growth inhibition and cell cycle arrest. METHODS: The recombinant retroviral vector pLp16SN was constructed by cloning the human p16 cDNA into the retroviral vector pLXSN. Retroviruses with or without the p16 gene were obtained by transfecting pLp16SN and pLXSN vectors into PA317 cells. Bcap-37 human breast cancer cells were infected with these retroviruses followed by selection with G418. The expression of p16 was detected by Northern and Western blots. Cell biological characteristics, including cell growth rate, cell cycle and tumorigenesis in nude mice were assessed. RESULTS: Both mRNA and protein expression of p16 in Bcap-37 cells transfected with retroviral vector containing the p16 gene were much higher than that in Bcap-37 cells transfected with empty vector or parental Bcap-37 cells. Cell overexpressing the p16 gene exhibited a slower rate of growth, a higher percentage of cells in the G1 phase, and smaller tumors in nude mice, compared with parental Bcap-37 cells and cells transfected with empty vector. CONCLUSION: Overexpression of the p16 gene could suppress the growth of Bcap-37 breast cancer cells by arresting the cell cycle at the G1 to S-phase.

Breast Neoplasms↗

PubMed2001

[The morphologic structure of vitreous hemorrhage and posterior vitreous detachment].

OBJECTIVE: To investigate the morphologic structures of vitreous hemorrhage that may be beneficial to its treatment. METHODS: The clinical data of 74 cases (79 eyes) with vitreous hemorrhage resulting from proliferative diabetic retinopathy, retinal vasculitis and retinal vein occlusion in December 1995 to January 2000 and having undergone vitrectomy were studied retrospectively, which focused on the morphologic characteristics of vitreous body by B-scan, surgical microscopy and surgical manipulation, to evaluate the relation of retina and vitreous body in order to discern the relationships among the retina, neovascular fibromembrane and the posterior hyaloid surface. RESULTS: Posterior vitreous detachment (PVD) in various degrees was observed in all the cases. The morphologic character was classified as the complete and partial vitreous detachment. The latter one can be subdivided into "V", "L" types and posterior vitreoschisis that was not rarely seen in the proliferative phase of ischemic retinopathy. CONCLUSIONS: To evaluate the characteristics of posterior vitreous detachment and vitreoschisis may provide valuable information for vitreoretinal surgery, elevate the rate of success and improve the prognosis of the surgery.

Adult↗

PubMed2001

[Studies on the change of extracellular matrix in the corneal tissue after photorefractive keratectomy in rabbits].

OBJECTIVE: To investigate the relationship between the haze formation and the change of extracellular matrix (ECM) in the corneal tissue after photorefractive keratectomy (PRK). METHODS: Twenty-four adult New Zealand White rabbits received PRK in the right eye for minus sign10.00 D ablation. According to the time of rabbits sacrifice, they were divided into 8 groups, and 3 rabbits were in each group. They were immediate, 24 hour, 1 week, 2 week, 1 month, 3 month, 6 month and 12 month groups. After surgery, the corneas were examined periodically by slip-lamp microscopy for haze formation. The right eyes were enucleated, and the corneas were studied with immunohistochemical evaluation for extracellular matrix, including collagen type I, type III, type VI, cellular fibronectin, tenascin and laminin. RESULTS: The incidence of haze was 100% after PRK. The formation of haze had a tendency from mild to severe, and along with the time prolongation, it gradually decreased and disappeared. The newly synthesized collagen type I, type III, type VI, cellular fibronectin, tenascin and laminin were deposited in the anterior corneal stroma. CONCLUSION: The formation of haze is closely related to the deposition of ECM elements in the anterior corneal stroma in the surgical area.

Animals↗

PubMed2001

[Myoid/myofibroblastic differentiation in dermatofibrosarcoma protuberans: a clinicopathologic study of six cases].

OBJECTIVE: The nature of myoid/myofibroblastic differentiation in dermatofibrosarcoma protuberans (DFSP) and its clinical and pathological significance were studied. METHODS: 124 DFSPs were reviewed by light microscopy. 6 cases with areas of myoid/myofibroblastic differentiation were assayed with immunohistochemical technique and electron microscopy was applied in two cases. RESULTS: Myoid/myofibroblastic differentiation occurred most commonly in fibrosarcomatous DFSP (FS-DFSP). It was recognized histologically as peripherally distributed or randomly scattered small eosinophilic nodules or short bundles, which were composed of bland spindle cells, closely resembling smooth muscle cells or myofibroblasts. Immunohistochemically, cells in myoid/myofibroblastic areas showed positive staining for alpha-SMA, MSA and vimentin, but negative for desmin and CD34. Electron microscopic study displayed the presence of microfilament bundles, focal dense bodies and micropinocytic vesicles, consistent with those of myofibroblasts. CONCLUSION: Myoid/myofibroblastic areas in DFSP possibly represents the hyperplasia of stromal myofibroblasts, rather than true myofibroblastic differentiation of the neoplastic cells.

Actins↗

PubMed2001

[Mutations of APC and K-ras gene in aberrant crypt foci from human colon].

OBJECTIVE: To analyze the mutations of K-ras and APC gene in normal colorectal mucosa, aberrant crypt foci (ACF), adenoma and colorectal carcinoma (CRC); To study the genetic alteration in ACF and its possibility of being a molecular marker of very early colon cancer and to explore the relationship of ACF and colorectal adenoma. METHODS: DNA from 35 CRC, 15 adenomas, 34 ACF and 15 cases of normal mucosa with mucous glands were isolated by micro-dissection. Direct gene sequencing of k-ras gene including codon 12, 13 and 61 as well as the mutation cluster region (MCR) of APC gene. RESULTS: Mutation frequency of k-ras gene in ACF, adenoma and carcinoma was 17.6% (6/34), 13.3% (2/15), and 14.3% (5/35) respectively, showing no difference between the three pathologic changes. The ras gene mutation sites in adenomas, carcinomas and 4 ACF were limited to codon 12 (GGT-->GAT), but in 2 ACF, they were located in codon 13 (GGC-->GAC). K-ras gene mutation in ACF was found more frequently in old patients and in patients with polypoid cancers. No mutations were found in codon 61 in three types of tissue. Mutation rate of APC genes in adenomas and carcinomas was 22.9% (8/35) and 26.7% (4/15) respectively, which was higher than that of ACF 2.9%, (P < 0.05). APC gene mutation in carcinomas was not correlated with age, location, size and differentiation. CONCLUSION: The morphological changes and gene mutation status are different in ACF and adenomas. ACF is a very early morphological lesion in the carcinogenesis of colorectal carcinoma. Therefore, ACF is considered to be the putative "microadenoma" where the development of colorectal carcinomas may be from "normal epithelium to ACF and then to carcinomas".

Adenoma↗

PubMed2001

[Ossifying fibromyxoid tumor of soft parts: a clinicopathological analysis of eight cases].

OBJECTIVE: To study the morphological characteristics and immunophenotype of ossifying fibromyxoid tumor of soft parts (OFT) with a discussion of its histogenesis. METHODS: The clinical, pathological and immunohistochemical features of 8 cases of OFT were evaluated. RESULTS: All 8 cases were middle to old aged patients, ranged from 43 -- 78 years (mean 63 years). Clinically, the majority presented as slowly growing painless masses that located in subcutis of the proximal extremities. Histologically, the tumor was characterized by the following three unique features which have diagnostic value. (1) The tumor was well circumscribed and encapsulated with an incomplete bony shell composed of metaplastic bone within the capsule in most cases; (2) The tumor parenchyma consisted of lobules of variable size and cellularity. The tumor cells within each lobule were rounded to short spindled with pale-staining or eosinophilic cytoplasm; (3) The tumor cells arranged in nests, cords, or laciform pattern, and were embedded in a characteristic fibromyxoid to collagenized stroma. Immunohistochemically, all 7 cases tested were positive for vimentin and NSE, while 6 cases expressed S-100 protein and 2 cases expressed desmin. Follow-up information showed recurrences in two patients 2 and 15 years after local excision. CONCLUSIONS: OFT is a distinctive soft tissue tumor of potentially low-grade malignancy which occurred predominantly in middle to old aged patients. The characteristic bony shell, the unique cytological appearance and arrangement of the cells are pathognomonic features of OFT. Our immunohistochemical result supported a Schwann-cell origin.

Adult↗

PubMed2001

[Detection of SYT-SSX fusion gene in paraffin-embedded tissues and its clinicopathologic significance for synovial sarcoma].

OBJECTIVE: To investigate the feasibility of detecting SYT-SSX fusion gene in paraffin-embedded tissues and its diagnostic significance for synovial sarcoma (SS). METHODS: Formalin-fixed, paraffin-embedded samples of 38 cases of SS and 40 cases of control tumors (including spindle cell sarcoma and metastatic adenocarcinoma) were retrived from archival materials. SYT-SSX fusion transcripts were detected in all samples by RT-PCR. House-keeping gene Porphobilinogen Deaminase (PBGD) was used to detect the quality of mRNA. RESULTS: PBGD mRNA was detected in 64 of the 78 tumor cases (82.1%). SYT-SSX fusion transcripts was detected in 33 of the 38 synovial sarcoma specimens. No SYT-SSX mRNA expression was found in control tumors. SYT-SSX mRNA expression rate reached 89.2% (33/37) in synovial sarcomas after exclusion of 1 case which was negative for both SYT-SSX and PBGD. In 33 SYT-SSX positive synovial sarcomas, 22 had SYT-SSX1 and 6 had SYT-SSX2 fusion transcripts. In 5 cases, the fusion type could not be distinguished. There was an association between SYT-SSX fusion type and histologic subtype. All 10 biphasic synovial sarcoma cases had the SYT-SSX1 fusion, whereas 12 of 18 monophasic synovial sarcoma had the SYT-SSX1 and 6 had the SYT-SSX2 fusion gene (P < 0.05). CONCLUSIONS: (1) Detection of SYT-SSX fusion gene in paraffin-embedded tissues was found to be a sensitive and specific method for the diagnosis and differential diagnosis of synovial sarcoma. (2) There was an association between SYT-SSX fusion type and histologic subtype.SYT-SSX2 fusion transcript could only be found in monophasic synovial sarcomas.

Adolescent↗

PubMed2001

[Effect of Astragalus membranaceus injection on megakaryocyte hematopoiesis in anemic mice].

OBJECTIVE: To assess the effect of Astragalus membranaceus injection (AMI) on megakaryocyte hematopoiesis in anemic mice and explore its mechanism. METHODS: Anemic models of mice were randomly divided into two groups: treatment group and anemic control group. Intraperitoneal doses of AMI (20 mg/(ml.20 g) q.d x 6) were given to the treatment group, and equal doses of physiological saline were given to the anemic control group. On days 8, 11 and 14 after treatment, blood platelet and bone marrow cells were determined, and the numbers of CFU-Meg (colony forming unit-megakaryocyte) and Meg-CSA (megakaryocyte colony-stimulating activity) were determined by using technique of hematopoietic progenitor cells culture in vitro. RESULTS: Serum Meg-CSA of the treatment group was significantly higher than that of the anemic control group. The abovementioned indices of the treatment group recovered to normal by day 11, which was markedly earlier than the day of recovery observed in the anemic control group. CONCLUSION: AMI can increase serum Meg-CSA of anemic mice and accelerate the recovery of megekaryocyte hematopoiesis after bone marrow suppression.

Anemia, Aplastic↗

PubMed2001

[Counting platelets by flow cytometry with platelet-specific antibody CD42a].

OBJECTIVE: To investigate a reference method for platelet (PLT) count. METHODS: 76 samples of daily whole blood of high, normal and low values in patients were collected and analyzed by flow cytometry (FCM) with CD42a monoclonal antibody that could combine specifically with the platelet membrane glycoprotein Ib-Ix (GPIb-Ix). PLT counts were analyzed by multiplying PLT/RBC ratio from FCM with the RBC that was counted by a calibrated blood analyzer. Meanwhile, the results of PLT from FCM were compared with that from blood analyzers SE-9500, NE-1500, CD-1600 or manual count. RESULTS: The within-replicate coefficient of variance(CV) of FCM was less than 4% for a high count(501 x 10(9)/L) and middle count(184 x 10(9)/L), the CV of low count(23 x 10(9)/L) was 11.432%, the between-replicate CV was 10.792%, and the overall reproducibility was less than 5%. Linearity was demonstrated well(r = 0.9975 P < 0.001) when the PLT counts were 20 x 10(9)/L-700 x 10(9)/L. PLT count was stable at room temperature within 6 hr. The carry-over was 0.87%. The results between FCM and SE-9500, NE-1500, CD-1600 or manual count was comparable (r > 0.975, P < 0.001). CONCLUSION: These findings confirm that FCM for PLT count is specific, accurate and precise and could be considered as a candidate of reference method for PLT count.

Antibodies, Monoclonal↗

PubMed2001

[Effects of low energy laser on tooth movement and remodeling of alveolar bone in rabbits].

OBJECTIVE: The aim of this study was to investigate effects of low energy laser on the experimental tooth movement and the remodeling of alveolar bone in rabbits. METHODS: A total of 42 white rabbits were chosen and randomized into one control group and six experimental groups, with 6 rabbits in each group. After anesthesia with 2% pentobaritone sodium, orthodontic appliances consisting of a coil spring connected bilaterally the upper first molar with the upper incisor by using a ligature wire. The force exerted at the time of insertion was approximately 80 g. The left side served as the control side, and the right side was the experimental side treated by receiving irradiation of low energy laser. The treatment periods of different groups lasted separately for 1, 3, 5, 7, 14, 21 days respectively. The displacement extent of teeth was measured by employing the computer image analyzing system. The results were analyzed statistically. Through HE staining, the histomorphological character of tissue around first molar was also investigated, and numbers of osteoclasts were counted. RESULTS: The displacement extent of teeth on the experimental side, which was irradiated by low energy laser, was more obvious than that of the normal control side. The difference was statistically significant 1, 3, 14, 21 days after the beginning of the treatment. Through histological observation under a light microscope, the osteoclasts and osteoblasts on the experimental side remained more active than those of the control side. There is significant difference in amount of osteoclasts between the experimental and the control sides, 3, 5 or 7 days after the treatment (P < 0.01, P < 0.05). CONCLUSION: The irradiation of low energy laser promote tooth movement and remodeling of alveolar bone.

Animals↗

PubMed2001

[Expression and significance of TGF-beta 1 in nasal polyps].

OBJECTIVE: To study the expression and significance of transforming growth factor-beta 1(TGF-beta 1) in nasal polyps. METHOD: Expression of TGF-beta 1 in nasal polyps from 34 patients and middle turbinates from 30 patients with deviation of nasal septum were prospectively studied with immunohistochemistry. Each tissue section was observed under optical microscope. RESULT: 1. The TGF-beta 1 positivity in extracellular matrix and in cells in the stroma was significantly higher in nasal polyps than in middle turbinates (P < 0.01). 2. The distribution and shape of TGF-beta 1 expressing cells in nasal polyps was similar to that of eosinophil, their positivities were significantly correlative (P < 0.05). 3. The positivity of TGF-beta 1 did not correlate with clinical type of nasal polyps (P > 0.05), eosinophil infiltration correlated significantly with clinical type of nasal polyps(P < 0.05). CONCLUSION: 1. The TGF-beta 1 may contribute to some of the pathologic changes observed in nasal polyps, such as thickening of the epithelial basement membrane and stromal fibrosis. 2. Eosinophils in nasal polyps represent a major source of TGF-beta 1. 3. Eosinophils infiltration may play a prominent role in the development and recurrence of nasal polyps.

Adolescent↗

PubMed2001

[Improved retrosigmoid approach operation in the treatment of trigeminal neuralgia].

OBJECTIVE: To explore the surgical method which can raise the treatment effect and reduce the complication occurrence of cerebellopontine angle (CPA) operation by retrosigmoid approach. METHOD: Have improved the retrosigmoid approach in the skin incision, the hole position of bone, the intracranial operation skills and the duramatral suture, etc. By the improved retrosigmoid approach, we used partial sensory rhizotomy (PSR) to cure 54 cases with trigeminal neuralgia (TN). RESULT: 52 cases got satisfactory treatment effect and no complication appeared. In the other 2 cases, the trigeminal nerve was not found in CPA. CONCLUSION: The CPA operation by retrosigmoid approach is dangerous relatively, it usually can cause some serious complication. But improved retrosigmoid approach can expose CPA sufficiently and is convenient to operate. On the other hand, it can decrease intracranial unexpected hemorrhage and complication occurrence.

Adult↗

PubMed2001

[The effects of perfluorodecalin on the anterior chamber angle and intraocular pressure in rabbits].

OBJECTIVE: To observe the effects of purfluorodecalin on the anterior chamber (AC) angle and intraocular pressure (IOP) in rabbits. METHODS: 0.05 ml perfluorodecalin was injected into right AC of 17 white rabbits and the same does of balanced salt solusion(BSS) was given to the controll group (6 rabbits). The IOP was measured before and 1, 2, 3, 4, 8, 12, 16 weeks after the injection. The treatment rabbits were sacrificed on the week of 1, 2, 4, 8 and 16 postoperatively. The eyes were enucleated and the histological examination was performed. RESULTS: There's no statistic IOP difference in the controll group before and after the injection of BSS. In treatment group, the IOP is similar to the preoperation level (11.62 +/- 6.35 mmHg) on the 7th postoperative day (10.82 +/- 3.17 mmHg, P < 0.05). A increment of IOP was measured at the week of 2 to 4, 8, 12, and 16 postoperatively (P < 0.01). Perfluorodecalin, having a 'fish eggs' or bubbles appearance, was noted in the inferior AC. Histopathologically, transparent single or fused vesicles and macrophages were accumulated at the AC angler in treatment rabbits. Some lymphocytes and neutrophils were aslo noted here. Trabeculae degenerated and intertrabecular space increased. CONCLUSIONS: The anterior chamber injection of perfluorodeclin could lead to a longstanding increase of IOP. Inflammatory reaction and trabeculae degeneration were seen in the anterior chamber angler.

Animals↗

PubMed2001

Experimental study on the effect of perfluorodecalin on rabbit corneas.

PURPOSE: To study the effects of perfluorodecalin on the cornea of the rabbit eyes. METHODS: Perfluorodecalin (0.05 ml/each) was injected into the anterior chambers of eighteen rabbit eyes. Corneal morphology and endothelial cells were monitored clinically by slit-lamp biomicroscope and specular microscope for 26 weeks. Animals were sacrificed in 1st, 2nd, 4th, 10th, 16th, 22nd, and 26th week after injection, respectively, and the corneas were examined under the light microscope. RESULTS: Perfluorodecalin droplets looking like "fish eggs" were found at about 1/4-1/2 of the corneal height in the inferior anterior chamber. Corneal opacification on the area contacted with perfluorodecalin was observed in five eyes five weeks after injection, and all in the 22nd week. Mutton fat KPs in one eye were seen in the 6th week firstly, and in all eyes in the 7th week. Corneal pannus formation in one eye was present in the 4th week, two eyes in the 5th week and three eyes in the 6th week. Retrocorneal fibrous membrane in one eye was detected at the 6th week and 3 eyes at the 7th week respectively. After injection of perfluorodecalin, endothelial cell density was significantly decreased (2,789 +/- 192 vs. 2,341 +/- 658, P < 0.01) and corneal thickness was increased. CONCLUSIONS: Perfluorodecalin injected into anterior chamber can lead to corneal damage and inflammatory reaction.

Animals↗