Illustrations of acute severe cases treated with acupuncture.
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Acupuncture Therapy↗
Biomedical subjects
X Zhu
Publications and source records attributed to X Zhu.
[Effects of monocyte-derived endothelin on acute cerebral infarction].
To investigate the mechanism that monocytes participate in ischemic brain injury, endothelin (ET) levels in plasma, supernatants of cultured monocytes in vitro and those pretreated by dexamethason (DXM) were assayed by radioimmunoassay in 31 patients with acute cerebral infarction and 16 patients with hypertension (served as control). Compared with the control group, the ET levels in supernatants of cultured monocytes increased and positively related with those in plasma and degree of neurological damage in the acute cerebral infarction group, while the ET levels in supernatants of cultured monocytes pretreated by DXM decreased. This suggests that intensifying the synthesis and release ET may be one of the mechanisms for the involvement of monocytes in the pathologic process of ischemic brain injury during acute cerebral infarction. DXM can improve this process by inhibition of producing monocyte-derived ET.
Adult↗
[Clinical evaluation of cardiac troponin I in ischemic heart diseases].
The detection of cardiac troponin I(cTnI), including serum or plasma, was evaluated in 114 patients with ischemic heart diseases or other heart diseases. The results were that the sensitivity of cTnI detection(qualitative analysis, cutoff is 0.2 ng.ml-1) was higher than that of creatine kinase(CK), creatine kinase-MB(CK-MB), alpha-hydroxybutyrate dehydrogenase(alpha-HBD), lactic dehydrogenase(LDH), and aspartic transaminase(AST) for diagnosing acute myocardial infarction (AMI) (93.2% vs 68.2%, 68.2%, 65.9%, 65.9%, and 75.0%; P < 0.05; respectively). The specificity of cTnI detection was higher than that of alpha-HBD, LDH, and AST(95.2% vs 81.0%, 73.8%, and 54.0%; P < 0.05; respectively) and similar to CK and CK-MB(95.2% vs 83.3% and 83.3%; P > 0.05; respectively). On the other hand, the sensitivity of cTnI in patients with unstable angina pectoris was 45.5%. It was higher than stable angina pectoris and lower than AMI. The results suggest that the cardiac troponin I is a better cardiac injury marker than other cardiac markers for diagnosing AMI.
Adult↗
Overexpression of CuZnSOD gene suppresses the growth of hepatocellular cancer cell line HepG2.
OBJECTIVE: To explore the inhibiting effect of superoxide dismutase (SOD) on the growth of hepatocellular cancer cell line HepG2. METHODS: By gene transfer technique, hepatocellular cancer cells (HepG2) were transfected with a retroviral vector containing human CuZnSOD cDNA. The elevated SOD gene expression of the transfected cells was compared with the parental and neo control cells. RESULTS: Compared to the control cells, cancer cells transfected with SOD gene showed an inhibited cell growth, a reduced number of cells in S phase and decreased clone forming ability in soft agar, as well as a smaller tumor size formed in nude mice. CONCLUSION: The overexpression of CuZnSOD gene could, to certain extent, suppress the cell growth of hepatocellular cancer cell line HepG2.
Carcinoma, Hepatocellular↗
Lipoprotein (a) concentration and apolipoprotein (a) phenotype in subjects with type 2 diabetes mellitus.
OBJECTIVE: To investigate the apolipoprotein (a) [apo(a)] polymorphism in the patients with type 2 diabetes mellitus and its relationship with complications. METHODS: In this study, we tested apo(a) phenotype via modified Utermann and Guo method in the 40 non-diabetic controls and 176 subjects with type 2 diabetes and analyzed the relationship between apo(a) phenotypes and micro- and macrovascular complications, including nephropathy, neuropathy, retinopathy, hypertension, coronary heart disease and cerebral infarction. RESULTS: Among the 40 non-diabetic controls, the frequencies of S3S2, S4 and S4S2 were 20%, 70% and 10% respectively and the serum lipoprotein (a) [Lp(a)] level was 0.08 +/- 0.07 mg/L. While the frequencies of S2, S3, S3S2, S4, S4S2, S4S3 were 18.18%, 20.45%, 17.05%, 34.09%, 4.55% and 5.68% in the diabetics and the Lp(a) concentration was 0.13 +/- 0.11 mg/L, with significant difference between the diabetics and non-diabetic controls. In comparison with the diabetics without complications, the frequency of apo(a) phenotype significantly differed in patients with nephropathy, nephropathy, hypertension, coronary heart disease and cerebral infarction except for diabetic retinopathy. In comparison with patients with S2 phenotype, the levels of fasting plasma glucose (FPG), 2-hour postprandial plasma glucose (2hPG) and glycated hemoglobin A1c (HbA1c) were lower in patients with S4 phenotype. The concentration of Lp(a) and urine albumin index (Alb/Cr) were significantly different among diabetics with different apo(a) phenotype, the highest being in patients with S2, secondly S3, and the lowest S4. CONCLUSION: There were significant differences in the frequency of apo(a) phenotype between subjects with type 2 diabetes and non-diabetic controls, and also in diabetics with or without microvascular and macrovascular diseases. The underlying linkage might be microalbuminuria and insulin resistance.
Adult↗
[Analysis of paracetamol, caffeine and chlorphenamine maleate in suxiaoshangfeng capsules by capillary gas chromatography].
A simple and rapid method for simultaneous determination of paracetamol, caffeine and chlorphenamine maleate in Suxiaoshangfeng capsule by capillary gas chromatography with n-hexadecane as internal standard has been reported. In the capsule, the three components can be separated on a 5% Ph-Me-Silicone column (2.65 microns x 0.53 mm x 10 m), with programmed column temperature from 180 degrees C to 230 degrees C at 8 degrees C/min, hold 6 min and then 230 degrees C to 260 degrees C at 10 degrees C/min, hold 10 min. Both the injector and FID detetor temperatures were 280 degrees C. N2 flow rate was 3.5 mL/min and H2 35 mL/min. The injection volume was 1 uL with splitting ratio of 1/25. These components were identified by their retention times and quantitatively determined by their peak areas. The calibration curves were, r = 0.9996 for paracetamol, r = 0.9984 for caffeine and r = 0.9996 for chlorphenamine maleate. The linear ranges were 4.0-20 g/L, 0.15-0.75 g/L and 0.080-0.40 g/L, respectively. The average recoveries were 99.62% (RSD = 0.40%, n = 5), 96.46%. (RSD = 1.32%, n = 5) and 98.55% (RSD = 0.65%, n = 5), respectively.
Acetaminophen↗
[Effects of the glucoprotein component of musk on functions of rat polymorphonuclear leukocytes activated by LTB4 in vitro].
To investigate the effects of musk-1, a glucoprotein component isolated from the water extract of musk, on some functions of rat polymorphonuclear leukocytes activated by LTB4, an in vitro incubation system with rat polymorphonuclear leukocytes was used. The superoxide anion production was determined by cytochrome C reduction, and the beta-glucuronidase and lysozyme release was quantitated by enzyme reactions in which phenolphthaleinglucuronic acid and micrococcus lysodeikticus were used as the substrates. In comparison with the control, musk-1 at final concentrations of 1 microgram/ml-100 micrograms/ml can increase the superoxide anion production by 28.7%-202.1% and decrease the beta-glucuronidase and lysozyme release by 3%-46% and 6%-32% respectively in rat polymorphonuclear leukocytes. It is concluded that musk-1 can significantly affect the functions of rat polymorphonuclear leukocytes activated by LTB4. One of the mechanisms of this anti-inflammatory action of musk may consist in the inhibition of lysosomal enzyme release.
Animals↗
[Clinical effect of tianma-cuzhi granules on senile vascular dementia].
OBJECTIVE: To assess the clinical efficacy of Tianma-cuzhi Granules (TMC) in treating senile vascular dementia(VaD) of the "sthenia of liver-yang" type. METHOD: TMC were given to thirty VaD patients three times a day of 0.5 g/bageach. The treatment course was one month and the patients finished two consecutive courses. RESULTS: TMC could markedly increase the mini-mental state examination (MMSE) and Hamilton depression scale(HDS) marks of VaD patients, and also contribute to improving certain indexes especially in abnormal cases. CONCLUSION: TMC have certain effects on senile VaD.
Aged↗
[Study on the in vitro and in vivo thrombolytic efficacy of bispecific antifibrin-antiurokinase monoclonal antibodies].
OBJECTIVE: To evaluate the thrombolytic efficacy of bispecfic antifibrin-antiurokinase monoclonal antibodies. METHODS: Murine hybrid hybridomas secreting bispecific monoclonal antibodies (bsMcAbs) were prepared by fusing a mutant hybridomas secreting antifibrin McAbs with spleen cells of murine immunized with urokinase. The binding power of bsMcAbs with fibrin or urokinase was determined by ELISA method. The in vitro thrombolytic efficacy was measured by the dissolving rate of 131I-plasma clot. The in vivo thrombolytic efficacy was measured by using the rabbit jugular vein thrombus model. RESULTS: 8 strains of bsMcAbs have been obtained. ELISA experiments showed that bsMcAbs have very strong binding power with fibrin or urokinase. The bsMcAbs bound with urokinase increased the in vitro thrombolytic efficacy of urokinase by 15.5%. The in vivo thrombolytic efficacy of bsMcAbs bound with urokinase was 1.6-2.1 times as high as urokinase. CONCLUSIONS: Monoclonal antibody-directed thrombolytic agents have advantages of high specificity and strong local thrombolytic efficacy. It is well worth going on further study.
Animals↗
[Prevalence of subgingival microbiota in Chinese using checkerboard DNA-DNA hybridization].
OBJECTIVE: To evaluate the prevalence of subgingival taxa of periodontal healthy persons and rapidly progressive periodontitis (RPP) patients using checkerboard DNA-DNA hybridization and to find the main pathogens in RPP. METHODS: Eighty four subgingival plaque DNA samples taken from 5 healthy subjects (H) and 6 RPP patients were hybridizated with 37 bacterial probes. RESULTS: The detection frequences of 6 bacteria (E. corrodens, et al.) were greater than 90%. Suspected periodontal pathogens, e.g. A. actinomycetemcomitans seratype b, T. denticola, F. nucleatum as nucleatum, B. forsythus, P. nigrescens, W. succinogenes, C. rectus, P. micros, S. intermedius and P. gingivalis were significantly more frequently found in RPP group than in H group. The mean counts of these pathogens were significantly more elevated in RPP group than in H group. CONCLUSION: It is suggested that periodontal destruction occurrence requires the pathogens in sufficient number at the site, RPP may be resulted from several pathogens.
Adult↗
[A study on the clinical curative effect by acupuncture for myofascial pain dysfunction syndrome].
OBJECTIVE: To study the acupuncture treatment for myofascial pain dysfunction (MPD) using infrared thermography and microcirculation. METHODS: The temperature of the skin on the TMJ regions, the blood vessels and the blood flow of the Nail Fold capillary loops, were observed in the group of the acupuncture He Gu and Min Yin point and the control group. RESULTS: There were high temperature of the skin on the TMJ regions, and enlarged blood vessels and increased blood flow of the Nail Fold capillary loops in the treatment group, with elimination of, pain and increase of mouth opening as compared with the control group. The effective rate of the acupuncture treatment of 477 cases of MPD is 93.1%. CONCLUSION: The He Gu and Min Yin points are sensitive point for the treatment of MPD.
Acupuncture Therapy↗
[Subclinical Sjögren's syndrome].
OBJECTIVE: To investigate various manifestations and treatment of subclinical Sjögren's syndrome (SS). METHODS: A long term clinical, laboratory and sialographic observations were performed in 24 patients with subclinical SS. Injectin of thymosin intramusularly was used to treat the patients and clinical effect of this treatment was evaluated. RESULTS: All of 24 cases had experienced recurrent parotid swellings (RPS) for 2-17 years (mean 7 years) before dry mouth and dry eyes occurred and all of them were misdiagnosed as chronic suppurative parotitis or sialadenitis. Follow-up showed that all of these patients developed SS. Vasculitis was found in 8 cases, purpura in 2 cases. The vascular involvement was relatively common in subclinical SS. Main sialographic findings was sialectasis of terminal ducts with irregular dilation of main duct. Frequency of RPS was markedly decreased in the patients treated with thymosin. CONCLUSION: The proposal of subclinical SS has theoretical value for understanding the entity and reclassification of chronic suppurative parotitis, and has guide value for diagnosis and treatment of SS in the early stage. Injection of thymosin is considered an effective therapy to reduce RPS in subclinical SS.
Adolescent↗
[Clinical observation of splenectomy on chronic idiopathic thrombocytopenic purpura in 63 patients].
OBJECTIVES: To investigate the effectiveness of splenectomy in patients with idiopathic thrombocytopenic purpura who failed to respond to conservative management. METHOD: Sixty-three patients were treated with splenectomy and followed up for 2 months to 16 years (average 4.7 years). RESULT: The patients had symptoms of bleeding and their platelet count on average was 24 x 10(9)/L. The first, 3rd, 5th and 7th day after operation, the average platelet count was 110 x 10(9)/L, 202 x 10(9)/L, 251 x 10(9)/L and 246 x 10(9)/L. Their platelet recovered to normal during a week in 51 cases (80.9%). The total effective rate was 86.9% after follow-up for 2 months. The recurrence rate was 17.9% in 39 cases after follow-up for longtime. CONCLUSIONS: The curative effect of splenectomy is better in children than in adults. In patients with increased platelet have a favourable prognosis. Splenectomy is safe and effective in the treatment of idiopathic thrombocytopenic purpura.
Adolescent↗
[An ultrasound biomicroscopic study on changes of the structure of ocular anterior segment after topical application of cycloplegia].
OBJECTIVE: To observe the changes of ocular anterior segment and the relation between the intraocular pressure (IOP) and these changes after the topical application of cycloplegia using ultrasound biomicroscope (UBM), especially to observe the changes of ciliary body thickness and ciliary body-lens distance. METHODS: The quantitative measurement of UBM and the measurement of IOP had been done in 48 normal eyes before and after the topical use of 2% homatropine solution. The results were statistically analyzed. RESULTS: In the parameter reflecting the change of the anterior chamber angle after using 2% homatropine, the trabecular iris angle decreased, the angle open distance 250 decreased, the iris thickness 1 increased, the anterior chamber became deeper and the iris-lens contact distance became shorter, and all these differences were statistically significant (P < 0.05). In the parameter reflecting the changes of ciliary body, the ciliary body thickness and the ciliary process thickness decreased, the scleral ciliary body angle increased, the iris-zonule distance decreased and the ciliary body-lens distance increased, and all these differences were significant statistically (P < 0.05). The IOP increased from 2.35 +/- 0.46 kPa to 2.43 +/- 0.47 kPa after the topical use of 2% homatropine, but the differences were not statistically significant (P > 0.05). CONCLUSIONS: After the topical application of cycloplegia, the anterior chamber becomes deeper, the anterior chamber angle becomes narrower, the ciliary body becomes thinner and moves backward and the ciliary body-lens distance increases. By using UBM the structure of ocular anterior segment in its moving state can be observed and these structures can be measured quantitatively. The ultrasound biomicroscopic imaging has its advantages in the morphological study of ocular anterior segment.
Adult↗
[Traumatic retina detachment and forensic medical identification].
It is reported the result of the examination and identification of 6 cases with retina detachment. The reasons, characteristics of the traumatic retina detachment and the problems about forensic medical identification were discussed.
Adolescent↗
[Effect of melatonin on mean blood pressure and heart rate in morphine withdrawal rats].
The model of morphine dependent rats was established by gradually increasing the doses of morphine by subcutaneous injection(from 5 mg.kg-1 to 50 mg.kg-1, 5 days). The effects of melatonin(MT) on the mean arterial blood pressure(mABP) and heart rate(HR) were studied in naloxone-precipitated rats. MT was found to inhibit the hypertention and tachycardiac syndromes in morphine withdrawal rats in dose-dependent manner. The concentration of serum norepinephrine(NE) was shown to be decreased by MT. Moreover, regitine was shown to inhibit the increase of mABP in morphine withdrawal rats, but can not inhibit the increase of HR. These results suggest that the inhibitory effect of MT on hypertension and tachycardiac syndromes in morphine withdrawal rats may be mediated by the inhibitory effect of the drug on the sympathetic system.
Animals↗
[Effects of leukotrienes on production of interleukin 6 from mouse peritoneal macrophages].
In the present study, effects of leukotrienes on IL-6 production by mouse resident peritoneal macrophages were explored with a bioassay method involving IL-6 dependent murine hybridoma B9 cell line. The results showed that LTB4, LTC4 and LTD4 can enhance the production of IL-6 from cultured peritoneal macrophages of mice. The optimal concentractions for LTB4, LTC4 and LTD4 were found to be 1.45 x 10(-6), 6.9 x 10(-8) and 8.05 x 10(-8) mol.L-1, respectively. The results suggest that peptide leukotrienes might play important role on IL-6 production in the local milieu of inflammation.
Animals↗
[Sensitivity and specificity of nested polymerase chain reaction in detecting Plasmodium vivax in infected mosquitoes].
AIM: To study the sensitivity and specificity of nested polymerase chain reaction for detection of Plasmodium vivax in infected mosquitoes. METHODS: Two pairs of primers specific to small subunit ribosomal DNA of P. vivax were used to amplify the specific SSUrDNA 121 bp fragment of P. vivax for detecting P. vivax-infected mosquitoes with nested PCR. RESULTS: Nested PCR could detect as few as 3 sporozoites in one mosquito or 1 infected mosquito mixed with a group of 99 normal ones. In contrast, no such specific 121 bp DNA band was detected in P. falciparum, P. cynomolgi, P. yoelii yoelii infected samples, nor in normal mosquito. CONCLUSION: The nested PCR technique we established showed high sensitivity and specificity.
Animals↗