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Biomedical subjects

X Yu

Publications and source records attributed to X Yu.

At least 343 records · Page 19Linked to original sources
PubMed1995

Peripheral GABAA receptor-mediated effects of sodium valproate on dural plasma protein extravasation to substance P and trigeminal stimulation.

1. The GABA transaminase inhibitor and activator of glutamic acid decarboxylase, valproic acid is being used for the treatment of migraine. Its mechanism of action is unknown. We tested the effects of sodium valproate and GABAA-agonist muscimol on dural plasma protein ([125I]-bovine serum albumin) extravasation evoked by either unilateral trigeminal ganglion stimulation (0.6 mA, 5 ms, 5 Hz, 5 min) or substance P (SP) administration (1 nmol kg-1,i.v.) in anaesthetized Sprague-Dawley rats. 2. Intraperitoneal (i.p.) injection of sodium valproate or muscimol, but not baclofen (< or = 10 mg kg-1, i.p.) dose-dependently reduced dural plasma protein extravasation caused either by electrical trigeminal stimulation (ED50: 6.6 +/- 1.4 mg kg-1, i.p., and 58 +/- 18 micrograms kg-1, i.p. for valproate or muscimol, respectively) or by intravenous substance P administration (ED50: 3.2 +/- 1.4 mg kg-1, i.p. and 385 +/- 190 micrograms kg-1, i.p. for valproate or muscimol, respectively). 3. Valproate (6.6 mg kg-1, i.p.) or muscimol (58 micrograms kg-1, i.p.) had no effect on mean arterial blood pressure or heart rate when measured for 30 min after i.p. administration. 4. The GABAA-antagonist bicuculline (0.01 mg kg-1, i.p.) completely reversed the effect of valproate and muscimol on plasma extravasation following electrical stimulation or substance P administration, whereas the GABAB-receptor antagonist, phaclofen (0.01-1 mg kg-1, i.p.) did not. Bicuculline or phaclofen, given alone, did not alter the plasma extravasation response after either electrical stimulation or SP administration. 5. Valproate decreased plasma extravasation following substance P administration in adult animals, neonatally treated with capsaicin by a bicuculline-reversible mechanism. This suggests that GABAA receptors are not found primarily on those afferent neurones or fibres which are sensitive to capsaicin treatment in neonatal rats.6. We conclude that sodium valproate blocks plasma extravasation in the meninges through GABAA mediated postjunctional receptors probably within the meninges. The dosages required are comparable to those used clinically. Agonists and modulators at the GABAA receptor may become useful for the development of selective therapeutic agents for migraine and cluster headache.

Animals↗
PubMed1995

Cardiac responses to induced lactate oxidation: NMR analysis of metabolic equilibria.

The role of lactate as a source of pyruvate oxidation in supporting cardiac work, energetics, and formation of oxidative metabolites was examined in normal myocardium. 13C- and 31P-nuclear magnetic resonance (NMR) spectra were acquired from isolated rabbit hearts supplied 2.5 mM [3-13C]lactate or [3-13C]pyruvate with or without stimulation of pyruvate dehydrogenase (PDH) by dichloroacetate (DCA). Similar workloads determined by rate-pressure products were noted with pyruvate (21,700 +/- 2,400; mean +/- SE) and lactate (18,970 +/- 1,510). Oxygen consumption was similar in all four groups with means between 19.0 and 22.2 mumol.min-1.g dry weight-1 (SE = 1.6-2.0) as was the ratio of phosphocreatine to ATP with means between 1.8 and 2.1 (SE = 0.1-0.6). Intracellular pH, determined from 31P-NMR spectra, was essentially the same with pyruvate (7.06 +/- 0.02) and lactate (7.05 +/- 0.04). 13C enrichment of glutamate was higher with lactate (92%) than with pyruvate (70%). Pyruvate plus DCA induced no change in glutamate content at 9-10 mumol/g, but 13C enrichment increased to 83%, while lactate plus DCA maintained enrichment at 90%. Levels of alpha-ketoglutarate were lower with lactate (1.81 mumol/g) than with pyruvate (2.36 mumol/g). Lactate plus DCA elevated glutamate by 60% with a proportional increase in alpha-ketoglutarate. Thus the balance between glutamate and alpha-ketoglutarate was affected by substrate supply only and not by PDH activation. The results suggest that the equilibrium between alpha-ketoglutarate and glutamate is sensitive to cytosolic redox state, an important consideration for 13C-NMR analyses that rely on glutamate.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1995

Fibroblasts, mononuclear phagocytes, and endothelial cells express monocyte chemoattractant protein-1 (MCP-1) in inflamed human gingiva.

Gingival inflammation is initiated by bacterial colonization of the tooth surface. It is characterized by infiltration of mononuclear cells, a feature of many forms of chronic inflammation. Monocyte chemoattractant protein-1 (MCP-1) is the predominant monocyte chemoattractant secreted by a variety of cells in vitro. We examined MCP-1 expression in chronic gingival inflammation by double antibody immunohistochemistry that utilized rabbit anti-MCP-1 antibody simultaneously with mouse monoclonal antibodies to specific cellular markers. MCP-1 mRNA expression by fibroblasts in inflamed gingival tissues was examined by in situ hybridization. We report here that in human chronic gingival inflammation the principal cell type expressing MCP-1 in dense inflammatory infiltrates is the mononuclear phagocyte. The cells expressing MCP-1 in moderately inflamed areas and in adjacent areas to inflammatory infiltrates are mononuclear phagocytes and fibroblasts, while in areas of fibroblastic hyperplasia, MCP-1 positive cells are predominantly fibroblasts. We also demonstrate that in moderately and highly inflamed areas, the extent of MCP-1 expression is greater than that in adjacent normal/mildly inflamed areas. As the degree of inflammation increased, there is also a concomitant increase in the number of mononuclear phagocytes. Furthermore, it is apparent that most of the infiltrating monocytes/macrophages are positive for MCP-1 in vivo. Our finding that MCP-1 expression is unambiguously identified in fibroblasts suggests that they can play a role in host defense by initiating the recruitment of monocytes. In addition, the expression of chemokines such as MCP-1 may represent a mechanism for amplification of inflammatory signals in gingival inflammation.

Animals↗
PubMed1995

Gene therapy against retroviral diseases.

Eventually, gene therapy may be a valid option for chronic viral infections, including retroviral infections. Human retroviral diseases fit two categories: (1) those that result from a monoclonal outgrowth of a human T-cell leukemia virus type I (HTLV-I)-infected cell, as in the case of adult T cell leukemia (ATL); and (2) those that appear to result directly from virus load rather than monoclonal outgrowth--such as tropical spastic paraparesis/HTLV-I associated myelopathy (TSP/HAM) and human immunodeficiency virus (HIV)-associated acquired immune deficiency syndrome (AIDS). For ATL gene therapy, corrective mechanisms directed at regulatory sequences rather than viral sequences may be most important, though perhaps anti-tax therapy would be useful. For TSP/HAM and AIDS, gene therapy directed to control virus replication may be most useful. For anti-retroviral therapy, one may use dominant negative mutants and a variety of other approaches that direct toxins or compete out viral regulatory gene signal sequences. For maximum benefit, such therapy should be directed to different essential genes (eg gag, pol, env, tat or rev) involved in the virus replication cycle and utilize different toxic approaches. A major impediment to the use of gene therapy for AIDS is our inability to transfect a significant fraction of target cells in vivo. Except for reconstituted mice, retroviral systems of animals have been under-utilized as models for gene therapy. Naturally occurring retroviral diseases of cats, goats, horses, and other species provide models for future development.

Acquired Immunodeficiency Syndrome↗
PubMed1995

[Physicochemical properties of a beta-glucan from Sclerotinia sclerotiorum].

Sclerotan (SSG) was an extracellular polysaccharide from Sclerotinia sclerotiorum by submerged fermentation. It had potential immunomodulating and antitumor activity. The SSG was a glucan composed of beta-linked D-glucoses. It was hard to dissolve in water under normal condition, but its aqueous solution had fine rheological properties. Its intrinsic viscosity [eta] hardly changed with ionic strenth. Change of its [eta] value was not remarkable between pH 1.88-12.36. Nevertheless, when the pH came to 13.32, the [eta] value decreased rapidly due to change of molecules conformation. Effect of temperature < or = 90 degrees C and heat treatment on apparent viscosity of SSG solution was minor.

Adjuvants, Immunologic↗
PubMed1995

Studies on kinetics of substrate utilization of hydrogen production from wastewater with immobilized cells of photosynthetic bacteria.

The kinetic characteristics of substrate utilization by immobilized cells of Rhodopseudomonas capsulata 386 and Rhodopseudomonas sp. D for H2 production was investigated. The results showed that substrate utilization did not proceed simultaneously with H2 evolution, H2 producing capacity of immobilized cells with agar was higher than that of alginate immobilized cells, but the appearance of maximum H2 producing activity was later than the last one. The kinetics of substrate utilization (glucose) by immobilized cells of strain D followed the first-order reaction, rate constant k value was 1.2 x 10(-2) L/h, analysis of macrokinetics indicated that substrate utilization by immobilized cells in H2 evolution process was governed by biochemical reaction other than diffusion transfer limitation, because of the Thiele modulus were 0.125 and 0.154 for immobilized cells with D = 3.6 mm and 4.4 mm particles, the corresponding effective factor were determined as 0.998 and 0.988, respectively. An immobilized cell bioreactor, fed by glucose and lactate, was employed for continuous H2 evolving system. It was found that hydrogen production were 0.659 L/d and 0.477 L/d in immobilized cells system of strain 386 and D when lactate used as H2 evolving substrate, while the volumetric H2 rate were all up to 1.0 L/L.d.

Diffusion Chambers, Culture↗
PubMed1995

Lipopolysaccharide induces exposure of fibrinogen receptors on human platelets.

The effect of lipopolysaccharide (LPS) on the exposure of platelet fibrinogen receptors was investigated. The results showed that: 1) LPS increased the binding of fibrinogen-gold complexes to platelets and the labels were primarily limited to shape-changed platelets; 2) LPS caused a dose-dependent rise in intracellular Ca2+ concentration in platelets; 3) LPS induced the activation of platelet protein kinase C (PKC) and the phosphorylation of glycoprotein llla (GPllla) which was inhibited by H-7. All these results suggest that stimulation of platelets with LPS causes a conformational change in glycoprotein llb/llla (GPllb/llla) through platelet shape change and/or phosphorylation of GPllla via PKC, which serves to expose the fibrinogen binding sites of GPllb/llla on human platelets.

Biological Transport, Active↗
PubMed1995

[The effect of retinoic acid on cell membrane and metastatic ability of mouse forestomach carcinoma cells].

We studied the effect of all-trans-retinoic acid (RA) on the expression of several surface lectin receptors and cell membrane fluidity of mouse forestomach carcinoma cell line (MFC) in vitro. The results showed that cells treated with RA manifested decreased expression of lectin receptors, increased membrane fludity and reduced spontanous metastasis. These rusults suggest that the effect of RA on tumor cell membrane may be one of the mechanisms involved in the alternation of cell metastatic phenotype.

Animals↗
PubMed1995

Prevalence of transformation zone Chlamydia trachomatis DNA and serum antibodies in Tanzanian gynaecological in-patients.

Chlamydia trachomatis DNA was detected in cervical transformation zone swabs of gynaecological in-patients from Tanzania by two different polymerase chain reactions (PCR), one targetting the endogenous Chlamydia trachomatis plasmid (pCTT1) and the other a chlamydia genus specific rRNA gene. In only 7/131 (5.3%) cervical samples specific amplification products were obtained, in 6 cases with both PCRs, and in one with the plasmid-PCR alone. A 255 nt sequence was determined from the two plasmid-PCR fragments and revealed only one mismatch against the prototype sequence. Antibodies against genus specific chlamydia antigens were detectable by indirect immunofluorescence with titres > or = 1:256 in 29.0% (38/131) of all patients and in 50.0% (11/22) of patients with pelvic inflammatory disease (P = 0.17). No statistically significant association of either chlamydia antibodies or chlamydial DNA with any clinical condition was observable.

Adolescent↗
PubMed1995

HBV surface antigen proteins with deletions in the preS region.

Four pairs of HBV surface antigen genes, in which the preS region was partially deleted, were constructed by the polymerase chain reaction (PCR). The comparison of the levels of the expression in mammalian cells of these genes and the ones constructed before, and the properties of these gene products showed that the missing of a part of the preS region did not affect the overall spatial structure of the S region and the surface localization of the preS region. The removal of the preS1 retention sequence (a. a. 2-19) alleviated significantly the shelter of the major antigenic determinants in the S region by the preS sequence. It was found that the long preS region seriously impaired the secretion of the surface antigen proteins from mammalian cells. In addition to the previously reported preS1 retention sequence, the preS1 sequence (a.a. 48-65) may also inhibit the secretion of the surface antigen proteins, whereas the preS2 region exerts no major influence on the retention of the large surface antigen protein. One of the expressed surface antigen proteins, in which the preS1 sequence (a.a. 21-47) and the S region were directly fused, deserves further study and may be developed into a new HBV vaccine which contains the preS1 binding site for hepatocyte receptors due to its stability, fine secretability and strong preS1 antigenicity.

Animals↗
PubMed1995

Diazepam, given postischemia, protects selectively vulnerable neurons in the rat hippocampus and striatum.

Following cerebral ischemia, certain populations of neurons degenerate. Excessive accumulation of excitatory amino acids in the synaptic cleft, activation of excitatory amino acid receptors, and influx of calcium into neurons play a key role in the development of ischemia-induced neuronal death. We hypothesized that neuroprotection may be achieved by enhancing inhibitory (i.e., gamma-aminobutyric acid, GABA) neurotransmission to offset excitation. Diazepam, a drug that increases GABA-induced chloride channel opening, was administered (10 mg/kg, i.p.) to rats 1 and 2 hr following 15 min of transient global ischemia, when hippocampal GABA levels, increased during ischemia, returned to basal. Rats were maintained normothermic during ischemia and became hypothermic following the injections of diazepam. Four days later, rats were sacrificed and the brains were examined for neuronal degeneration and the presence of GABAA receptors labeled by 35S-t-butylbicyclophosphorothionate (35S-TBPS). There was substantial neuroprotection of striatal neurons and pyramidal neurons in the CA1 area of the hippocampus. In addition, diazepam prevented the loss of 35S-TBPS binding sites in the striatum and in the dendritic fields of the CA1 hippocampus following ischemia. Since hypothermia, itself, is neuroprotective, we determined if hypothermia was required for the ability of diazepam to produce neuroprotection. Diazepam was microinjected into the CA1 hippocampus 1 and 2 hr following ischemia, and rats remained normothermic. Four days later, diazepam still produced substantial protection of hippocampal neurons. Thus, postischemic hypothermia may have contributed to the neuroprotection by diazepam when it was administered systemically, but the neuroprotective effect of diazepam did not require hypothermia. We conclude that delayed enhancement of GABAergic neurotransmission directly at the site of vulnerability following an ischemic event protects the vulnerable neurons from death.

Animals↗
PubMed1995

Effect of restorative materials on cuspal flexure.

The purposes of this study were (1) to establish a methodology for determining surface strains in two locations of the same tooth under intact, prepared, and restored conditions and (2) to compare the effects on stiffness of different restorative materials in a tooth subjected to cuspal loading. Two linear strain gauges were mounted on each of 30 extracted maxillary premolar teeth. Teeth were mounted in poly(methyl methacrylate) resin and randomly assigned to one of three study groups according to the restorative material and application technique to be used. Statistical analysis indicated a statistically significant interaction between restorative material and tooth condition at both proximal and buccal sites and a statistically significant difference in stiffness between teeth restored with Tenure/Marathon V and those restored with either amalgam or Scotchbond 2/P-50 at the proximal site. Results suggest that the methods employed provide a useful, nondestructive means of testing the same tooth under various conditions.

Analysis of Variance↗
PubMed1995

[The effect of intra-aortic balloon pumping in case of left main coronary artery stenosis].

In order to improve perfusion of coronary artery in case of coronary artery stenosis, effect of intra-aortic balloon pumping (IABP) on coronary blood flow (CBF) was investigated. To this end hemodynamic parameters of heart functions with and without stenosis were analysed under IABP. Hemodynamic effect of IABP was obviously influenced by the coronary artery stenosis.

Animals↗
PubMed1994

Killing of primary CD4+ T cells by non-syncytium-inducing macrophage-tropic human immunodeficiency virus type 1.

Understanding the mechanism by which human immunodeficiency virus type 1 (HIV-1) kills CD4+ T lymphocytes is important to the development of therapeutic and prophylactic strategies. Recent studies have indicated that, in some cases, progression to AIDS is associated with the appearance of syncytium-inducing, T cell line-tropic HIV-1 variants. Nevertheless, approximately 50% of subjects with AIDS harbor only non-syncytium-inducing, macrophage-tropic (NSI-M) variants of HIV-1. In most asymptomatic patients, NSI-M HIV-1 isolates are the predominant virus type found. We report here that cytopathicity of NSI-M HIV-1 for primary CD4+ T lymphocytes can be directly detected in vitro. The extent of CD4+ T-cell killing was not completely correlated with the rate of viral replication, suggesting that other characteristics of HIV-1 contribute to its cytopathicity. Our findings suggest that: (i) direct killing by NSI-M HIV-1 may contribute to CD4+ T-lymphocyte depletion in vivo, and (ii) the determinants of HIV-1 cytopathicity for CD4+ T lymphocytes and cell tropism or syncytia-forming ability are not necessarily tightly linked.

Acquired Immunodeficiency Syndrome↗
PubMed1994

The Escherichia coli RuvB branch migration protein forms double hexameric rings around DNA.

The RuvB protein is induced in Escherichia coli as part of the SOS response to DNA damage. It is required for genetic recombination and the postreplication repair of DNA. In vitro, the RuvB protein promotes the branch migration of Holliday junctions and has a DNA helicase activity in reactions that require ATP hydrolysis. We have used electron microscopy, image analysis, and three-dimensional reconstruction to show that the RuvB protein, in the presence of ATP, forms a dodecamer on double-stranded DNA in which two stacked hexameric rings encircle the DNA and are oriented in opposite directions with D6 symmetry. Although helicases are ubiquitous and essential for many aspects of DNA repair, replication, and transcription, three-dimensional reconstruction of a helicase has not yet been reported, to our knowledge. The structural arrangement that is seen may be common to other helicases, such as the simian virus 40 large tumor antigen.

Algorithms↗
PubMed1994

Mouse hepatitis virus gene 5b protein is a new virion envelope protein.

Highly purified radiolabeled mouse hepatitis virus (MHV) A59 contained a previously overlooked protein which coelectrophoreses with the gene 5b product immunoprecipitated from infected cells. The gene 5b protein is post-translationally acylated. Rabbit antibody raised against a recombinant gene 5b protein expressed in Escherichia coli neutralized viral infectivity in the presence of complement, although not in the absence of complement. Immunofluorescent staining of MHV-infected cells with two anti-peptide antibodies revealed that the gene 5b product is membrane-associated and is transported to the cell surface, findings consistent with the prediction of a membrane-spanning segment in the gene 5b polypeptide. These results suggest strongly that the gene 5b polypeptide represents a new MHV virion envelope protein which is homologous to the TGEV ORF 4 and IBV 3c proteins.

Antigens, Viral↗