[Changes in plasma endothelin and atrial natriuretic peptide in patients with diabetes mellitus].
Explore the source record for details and available documents.
Adult↗
Biomedical subjects
X Yu
Publications and source records attributed to X Yu.
[Study on measures to increase diagnostic accuracy of FNAC of breast masses].
OBJECTIVE: To study the measures capable of increasing the diagnostic accuracy, reducing misdiagnosis as well as giving a full play to fine needle aspiration cytology(FNAC), in the diagnosis of breast masses, and to standardize the diagnostic report form. METHODS: Totally 1629 cases of FNAC were performed and 444 cases of them were checked with the histological diagnosis. RESULTS: The sensitivity of diagnosis of malignant tumors in 307 cases, the specificity of diagnosis of benign lesions in 137 cases and the overall accuracy of diagnosis were 95.8%, 98.5% and 96.6% respectively. The false negative rate, potential false positive rate and the overall misdiagnostic rate were 4.2%, 1.5% and 3.4% respectively, while no case of false positive diagnosis was found. CONCLUSIONS: We believe that: (1) It is very important to use a fine aspirator, to make smears of high quality, to carry out an integrated working procedure and to put into effect a conscientious check over cytological appearance with histological diagnosis. (2) The diagnositc report form introduced in "the uniform approach to breast fine needle aspiration biopsy" by Bethesda specialists is worthy of reference. (3) FNAC can further play important roles in the diagnosis and treatment of breast masses.
Biopsy, Needle↗
[Experiences in management of acute necrotic pancreatitis].
To establish a treatment program for patients with acute necrotic pancreatits (ANP), we treated 211 patients with ANP from 1973 to 1995. 122 patients in group A were treated mainly by early operation before 1992 and 75 in group B, by early nonoperativemethod. The results showed that the occurrance of complications (ARDS, renal failure, heart failure, pancreatic abscess, intestinal fistula) was lower than that in the group B (P < 0.01). Also the mortality in the group B (10.67%) was decreased as compared with that (22.95%) in the group A. We suggested that the nonoperative treatment with rigid criterion for ANP might be recommended first. An intensive nonsurgical management of ANP, surgical indications during nonoperative period and complete program of management for ANP were discussed.
Adult↗
[Effects of the double chamber intra-aortic balloon pumping on the renal circulation].
The effects of the double chamber intra-aortic balloon (DIAB) and the single chamber intra-aortic balloon (SIAB) on the hemodynamic parameters in the kidney were studied. No negative effect of both cases was observed. Before and after removing the renal nerve system, the DIAB was used and the results had been compared. The pulse produced by pumping activized the renal nerve system and reduced the renal vascular resistance. Therefore the reduction of the renal vascular resistance is an important reason for increasing the renal blood flow during intra-aortic balloon pumping.
Animals↗
[Clinical application of ultrasound(US)-guided percutaneous microwave coagulation therapy for liver cancer].
OBJECTIVE: To evaluate clinical application of US-guided percutaneous microwave coagulation therapy for liver cancer. METHODS: 32 patients with 57 nodules of liver cancer were treated, 57 nodules were coagulated by 92 times at 182 different points. Output of one coagulation was 60 W last mg 240-300 sec. RESULTS: The follow-up period was 5-18 months, averaging 10.4 months. 29 patients remained alive, 3 patients died. After treatment, 85.9%(49/57) of the nodules decreased in size; color blood flow signal disappeared in 78.2(36/46) nodules on color and energy Doppler scans; and there was no enhancement in 76.4%(25/34) tumors on enhanced CT scans. In 13 patients with increased AFP level before therapy, it dropped to normal after treatment in 11 patients. Of 27 patients whoes a good general condition was good, 19 patients gained bodies weight. Repeated biopsy in 14 cases showed complete tumor necrosis with fibrosis in 12. CONCLUSION: US-guided percutaneous microwave coagulation therapy is able to induce total tumor necrosis of liver cancer in most cases. It can be expected as an important non-operative therapy of liver cancer.
Adult↗
[Temperature measuring and heating controlling in transurethral prostate thermotherapy system].
This paper introduces the system construction of Transurethral Prostate Thermotherapy Device. The temperature measuring device, I/O interface circuit and the programming principle of PID controlling system is illustrated also.
Algorithms↗
[Purification and properties of inulinase from Kluyveromyces sp. Y-85].
The crude endocellular inulinase from Kluyveromyces sp. Y-85 was purified to two components, designated as EI and EII, using PEG6000-phosphate buffer extraction, (NH4)2SO4 fractionation, DEAE chromatography and gel filtration (Protein-PAK); The crude exocellular inulinase from this strain was purified to Eexo by means of PEG6000-phosphate buffer extraction, double DEAE-Sephace chromatography, Sephadex G-150 gel filtration. EI, EII and Eexo were demonstrated to be homogeneous by Waters 650E protein purification system. Their molecular weights are 42 kD, 65 kD and 57 kD, respectively. All the inulinases were glycoproteins containing a saccharide (from 25% to 35%) and belonged to the endo-inulinase. In addition, EI, EII, Eexo were optimally reactive at pH4.6, 4.5, 4.6 and at 52 degrees C, 52 degrees C, 55 degrees C, respectively. Ag+, Hg2+ and PCMB inhibited these enzymes' activity strongly. The products of raw inulin extracted from Helianthus tuberosus hydrolyzed by these three enzymes were fructose (86.5%) and glycose (13.5%).
English Abstract↗
[Physiological effects of cabin temperature decrease on human].
To study effects of cabin temperature on human body, changes in rectal temperature, local skin temperature, local skin heat fluxes and skin infrared thermogram were observed in 5 male subjects dressed in different thermal insulation clothings under 20 degrees C, 15 degrees C and 10 degrees C cabin temperatures. The results showed that, average skin temperature and tip temperature obviously dropped, and skin heat fluxes increased with falling of cabin temperature, and changes of local skin infrared thermogram were significant. Subjective feeling were comfortable and cool at cabin temperature of 20 degrees C and 15 degrees C respectively. While subjective feeling was cold and there was a thermal debt of body 144 kJ/m2 at 10 degrees C which indicated that it was slightly cold but tolerable. The results may be valuable in determining the lowest tolerable limit of cabin temperature.
Acclimatization↗
[Comparison of evaluation methods on changes of facial microcirculation during induction of motion sickness].
In order to increase the accuracy of motion sickness grading criteria, infrared thermography and Laser Doppler microcirculation blood-flow detector were used in 60 healthy male individuals: before, immediately, and 20 min after paralleled-swing stimulation. Facial skin temperature and microvessel blood-flow were recorded. The results showed that facial skin temperature and blood flow in facial microvessels fell together with the appearance of pallor. However, the degree of pallor observed with naked eye was inconsistent with that found by thermography or facial blood flow. It suggests that the accuracy of grading might be improved with the use of thermography or measurement of facial microvessel blood flow.
Face↗
[Yu Jinghe's Chronicle].
The life and origin of medical learning from family of Yu Jinghe, renowned doctor of the Qing Dynasty, are described here in the form of chronicle. He was born in Yixing, Jiangsu. His parent died when he was a boy and he joined the Taiping Army for 5 years with difficult experiences. Later, he moved to Menghe and learned pharmacology from his elder brother. His works include Yu's Annotation on Shanghanlun Yi, Collection of Case Records on External Diseases, Collection After Practising Hour etc. Moreover, Yu's descendents and his close relationship with other modern celebrated doctors such as Ding Ganren and his son Ding Zhongying, and Yun Tieqiao are also mentioned.
China↗
[Human papillomavirus type 16 E6 oncogene and expression of P53, RB and PCNA in human cervical carcinoma].
The aim of this study was to get some insights into the relationship between human papillomavirus type 16 E6 transforming gene and the expressed products of tumor suppressor gene, the P53, RB and proliferation cell nuclear antigen (PCNA) in human cervical carcinoma. 44 formalin-fixed paraffin-embedded cervical carcinoma sections were screened for P53, RB and PCNA by immunohistochemical assay with their monoclonal antibodies, and for the presence of HPV16 E6 ORF by in situ hybridization with HPV16 E6 DNA probe we have successfully labeled. The presence of HPV16 E6 gene was detected in 27 of 44 specimens (61.3%), including 8 of P53 protein positive (29.63%), 14 of RB protein positive (52.85%), and 20 of PCNA positive (74.07%). Of 17 HPV16 E6 negative cases, there were 7 of P53 protein positive (41.17%), 9 of RB protein positive (52.94%), 12 of PCNA positive (70.58%). There were no HPV16 E6 gene in control group. In positive cases PCNA only expressed in base cells. Our date indicated that HPV16 E6 ORF and PCNA had significantly related to cervical carcinoma. We could not find the relationship between HPV16 E6 ORF and P53, RB, PCNA in cervical carcinoma. There were some HPV16 E6-positive cases, obviously with strong P53, RB immunostaining in cytoplasm but not in nuclei, PCNA immunostaining was strong at the edge of the cervical carcinoma cells than in nuclear of them.
Carcinoma↗
[Detection of human papillomavirus DNA sequence in esophageal carcinoma using polymerase chain reaction].
Human Papillomavirus (HPV) DNA sequence in 68 paraffin-embedded specimens of esophageal carcinoma collected from high-risk Shantou City was detected and typed by polymerase chain reaction (PCR) technique. The results showed a 66.18% (45/68) positive rate of HPV DNA. Among these esophageal carcinoma specimens, 44 out of the 45 positive specimens were squamous cell carcinoma and one was adenosquamous carcinoma. The HPV DNA types detected were mostly HPV-6, -11, and -16. HPV-6 DNA was found in 19 (27.94%) of the 68 specimens, HPV-11 DNA in 25 (36.76%) and HPV-16 in 19 (27.94%) specimens. Among these three types, the difference gave no statistical significance (P > 0.05). But HPV-18 DNA was detected in only 6 (8.82%) specimens and the HPV in other 6 specimens was unidentified. It should be noted that as many as 24 (53.33%) of the 45 positive cases had multi-infection (coinfection of 2 or 3 types). Our results suggested that a higher detectable rate of HPV DNA was found in Shantou City as a high-risk region and that HPVs might be closely associated with the pathogenesis of the esophageal carcinoma.
Adult↗
[A seroepidemiological survey: antibody to HTLV-1 in sera from various populations in Guangdong Province].
A seroepidemiological survey of HTLV-1 infection in Guangdong Province was reported. 2224 serum samples from various populations were collected and antibodies to HTLV-1 in sera were detected with indirect immunofluorescent assay. Total seropositive rate was 1.57% (35/2224). The antibody positive rates of HTLV-1 in sera from healthy individuals (n = 1810), blood donors (n = 248), patients with T cell leukemia (n = 109) and patients with neurological diseases (n = 57) were 1.27%, 0.40 %, 7.30% and 5.26% respectively. There was a significant difference between the patients with T cell leukemia and the healthy individuals (P<0.005).
Antibodies, Viral↗
[Detection of minimal residual disease after allogeneic bone marrow transplantation in chronic myeloid leukemia].
OBJECTIVE: To detect minimal residual disease (MRD) after allogeneic bone marrow transplantation (allo-BMT) in chronic myeloid leukemia (CML). METHODS: M-bcr/abl mRNA was assayed by reverse transcriptase polymerase chain reaction (RT-PCR) in bone marrow cells from 46 successfully sibling marrow engrafted CML patients. RESULTS: About 70% of the patients achieved genetic complete remission in 3 months post allo-BMT. Four patients were M-bcr/abc mRNA positive at 1.5 to 2 months post allo-BMT and turned to be negative at 3 to 9 months post allo-BMT. One patient was still M-bcr/abl(+) after disease-free survival(DFS) for more than 6 years, while another one was M-bcr/abl(-) after DFS for more than 4 years. CONCLUSION: RT-PCR is so far the most sensitive method for MRD detection in CML, but its limitation should not be ignored.
Adolescent↗
[Study on conversion of RARalpha/PML fusion gene in acute promyelocytic leukemia].
OBJECTIVE: To analyze the conversion of RARalpha/PML gene in acute promyelocytic leukemia (APL) patients before and after treatment with all-trans retinoic acid (ATRA) followed by intensive consolidation chemotherapy (ICC) and allogeneic bone marrow transplantation (allo-BMT). METHODS: RARalpha/ PML fusion gene was detected in 22 APL patients before and after treatment by reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: RARalpha/PML fusion gene was positive in 75% of the patients after achieving complete remission with ATRA, and turned negative in 83% of the patients after ICC. The durations of conversion to the RT-PCR negative status varied from 1 to 39 months. Ten patients received allo-BMT, and all of them were RARalpha/PML fusion gene negative in 4 months post allo-BMT. CONCLUSION: APL patients could achieve biological remission after ICC and allo-BMT, and the latter seemed to eliminate residual leukemic cells sooner in vivo than the former did.
Adolescent↗
[Determination of sulpride in human plasma by high performance liquid chromatography].
This paper describes a reliable method for the pharmacokinetic study of Sulpride in human plasma by reversed-phase high performance liquid chromatography. To compensate the loss of Sulpride during the extraction procedure we used an internal standard very similar in chemical structure and UV absorbance to those of Sulpride. The mobile phase was methanol-water-acetic acid (60:30:1) with a flow rate of 1.2 mL/min. A UV detector was used at 290 nm. The linear range was 5-100 mg/L and the detectable limit was 1.0 mg/L. The recovery and RSD were 97.95%-99.96% and 2.6%-5.1% respectively. The results showed that this method is a sensitive and accurate one which makes the pharmacokinetic study of Sulpride possible. If the concentration was too low to be detected by UV monitor, a fluorescence detector could be used with the excitation wavelength at 299 nm and emission at 342 nm. We analyzed the plasma samples from 30 day-treated psychotic patients and got the satisfactory results.
Antidepressive Agents↗
[The effect of alkalinity of methylation reagent on isomerization and degradation of polyunsaturated fatty acids with pyrolysis gas chromatography].
Simultaneous pyrolysis and methylation gas chromatography (SPM-GC) is a fast analytical method which was developed in recent years. However, the strong alkalinity of tetramethylammonium hydroxide (TMAH) may result in the isomerization and degradation of polyunsaturated fatty acids in oils and fats during analysis. This paper describes the application of SPM-GC to polyunsaturated fatty acids in soybean oil and the isomerization and degradation may be prevented by choosing the optimum proportion of TMAH to methanol which is 1:50 (pH 8.8). With this chosen optimum proportion there were no detectable isomerized and degraded peaks in the chromatograms. Pyrograms were obtained by interfacing a Curie-point pyrolyser to a Shimadzu GC-14A gas chromatograph equipped with a flame ionisation detector. A capillary column (FFAP) was used. All of the pyrolysis products were identified by mass spectrometry which was carried out with a Varian 3400 gas chromatograph interfaced to a Finnigan SSQ 710 mass spectrometer using electron impact ionisation (70 eV). This method is simpler and more convenient than both the methods "neutralization of acid" and "simultaneous injection of the sample and a short chain fatty acid methylester" reported in the previous papers.
Chromatography, Gas↗
In vitro metabolism of simvastatin in humans [SBT]identification of metabolizing enzymes and effect of the drug on hepatic P450s.
Simvastatin (SV) is a lactone prodrug used for the treatment of hypercholesterolemia. Upon incubation of SV with liver microsomal preparations from human donors, four major metabolic products were formed (3'-hydroxy SV, 6'-exomethylene SV, 3',5'-dihydrodiol SV, and the active hydroxy acid, SVA), together with several minor unidentified metabolites. The 3',5'-dihydrodiol SV, a new metabolite, was inactive as an inhibitor of HMG-CoA reductase. Kinetic studies of SV metabolism in human liver microsomes suggested that the major NADPH-dependent metabolites (3'-hydroxy SV, 6'-exomethylene SV, and 3',5'-dihydrodiol SV) were formed with relatively high intrinsic clearances, consistent with the extensive metabolism of SV observed in vivo. Based on four different in vitro approaches, namely 1) correlation analysis, 2) chemical inhibition, 3) immunoinhibition, and 4) metabolism by recombinant human P450, it is concluded that CYP3A is the major enzyme subfamily responsible for the metabolism of SV by human liver microsomes. Both CYP3A4 and CYP3A5 were capable of catalyzing the formation of 3',5'-dihydrodiol, 3'-hydroxy, and 6'-exomethylene metabolites. However, CYP3A4 exhibited higher affinity (> 3 fold) for SV than CYP3A5. Also, the studies indicated that CYP2D6, CYP2A6, CYP2C8, CYP2C9, CYP2C19, CYP1A2, and CYP2E1 did not play significant roles in the metabolism of SV in vitro. Over the concentration range of 0-40 microM, SV inhibited the activity of CYP3A, but not the activities of CYP2C8/9, CYP2C19, or CYP2D6 in human liver microsomes. The inhibition of hepatic midazolam 1'-hydroxylase, a CYP3A marker activity, by SV was competitive with a Ki value of approximately 10 microM. SV was > 30-fold less potent than ketoconazole and itraconazole as an inhibitor of CYP3A. Under the same conditions, SVA, the hydrophilic hydroxy acid form of SV, did not inhibit CYP3A, CYP2C8/9, CYP2C19, or CYP2D6 activities. The results suggested that the in vivo inhibitory effects of SV on the metabolism of CYP3A substrates likely would be less than those of ketoconazole and itraconazole at their respective therapeutic concentrations. In addition, metabolic activities mediated by the other P450 enzymes tested are unlikely to be affected by SV.
Anticholesteremic Agents↗