Search PubMed⌕ Search

Biomedical subjects

X Yang

Publications and source records attributed to X Yang.

At least 235 records · Page 13Linked to original sources
PubMed2001

[Studies of a new adsorbent cross-linked agar beads entrapped attapulgite clay for hemoperfusion].

The preparation and properties of a new adsorbent cross-linked agar beads entrapped attapulgite clay (CAA) are reported in detail. Medicinal attapulgite clay made in China was encapsulated with agar, shaped in organic solvent, and cross-linked by epichlorohydrin. The products withstood autoclaving at 121 degrees C for half an hour with no breakage, and had a good selective adsorption ability for some model compounds and medicaments such as methylene blue and phenothiazine drugs. Preliminary investigations and SEM photographs indicate that the CAA as an adsorbent is relatively hemocompatible for hemoperfusion.

Agar↗
PubMed2001

[Overexpression of p27(KIP1) induced by Bak gene leads to the arrest in G(1) phase of HCC-9204 cell line].

OBJECTIVE: To explore whether p27(KIP1) plays an important role in prolonging cell cycle in G(1) phase and leading to apoptosis of HCC-9204 cells. METHODS: A model of Bak-induced cell cycle arrest in G(1) phase and subsequent apoptosis was established. p27(KIP1) was obtained from the model and sequenced afterwards. A zinc inducible p27(KIP1) stable transfectant was constructed. The effects of inducible p27(KIP1) on cell growth and cell cycle arrest were examined in control pMD and pMD-KIP1 transfected HCC-9204 cells. Western blot was performed to evaluate the expression of p27(KIP1). RESULTS: The cell growth was reduced by 35% upon 48h of p27(KIP1) induction with zinc treatment as determined by trypan blue exclusion assay. p27(KIP1) caused cell cycle arrest at 24h after induction, with 40% increase in G(1) population. CONCLUSIONS: Bak may induce cell cycle arrest in G(1) phase through up-regulating expression of p27(KIP1). The inducible p27(KIP1)-expressing cells provide a model to assess p27(KIP1) function.

Apoptosis↗
PubMed2001

[Trihalomethane formation potential (THMFP) of soils in Guangdong Province].

Trihalomethane formation potentials were analyzed with the lixivia of typical soil samples in Guangdong Province. The results showed that the bulk THMFP contents of soil lixivia (b-THMFP) range from 0.7 to 36.8 micrograms/g with a median value of 10.6 micrograms/g, and the THMFP contents of 0.45 micron filtered soil lixivia, representing the THMFP contents of dissolved organic matter (d-THMFP), from 0.5 to 21.2 micrograms/g with a median value of 3.9 micrograms/g. Sample 19 (Calcareous soil) had the highest b-THMFP and d-THMFP while sample 20 (Purple soil) had the lowest b-THMFP and sample 5 (Latored soil) had the lowest d-THMFP. In general, suspended organic matter had great contribution to the THMFP. The physico-chemical properties, such as organic matter contents and contents of various oxides, as well as soil genetic horizon and vegetation are main factors dominating the THMFP levels in the soil.

Soil Pollutants↗
PubMed2001

Association of the ADH2 genotypes with skin responses after ethanol exposure in Japanese male university students.

BACKGROUND: A contribution of the alcohol dehydrogenase-2 (ADH2) polymorphism to alcohol sensitivity and alcohol drinking behavior is still controversial. In this study, we examined the effects of the ADH2 genotypes on skin reactions to ethanol and habitual alcohol intake among Japanese male university students, controlling for the effects of the low Km aldehyde dehydrogenase (ALDH2) genotype, as an extension of our previous study. METHODS: The study subjects were 357 Japanese male students [average age (mean +/- SD) was 23.7 +/- 3.0 years] in a medical university. The subjects completed a questionnaire regarding self-reported alcohol-associated symptoms and alcohol-drinking behavior. The ADH2 and ALDH2 genotypes were determined through digestion of polymerase chain reaction products by restriction enzymes. All subjects participated in the ethanol patch test. We observed skin responses at 0, 5, 15, and 20 min after removal of the tape. RESULTS: Among the ALDH2*1/*1 genotypes, only some subjects with ADH2*1/*2 or ADH2*2/*2 exhibited a positive response, which increased with increasing time after the removal. However, none of comparisons between the different ADH2 genotypes reached statistical significance. Among the ALDH2*1/*2 genotypes, those with ADH2*1/*2 or ADH2*2/*2 showed a significant increase in response with increasing time after the removal and revealed a significantly higher positivity rate at 15 min than those with ADH2*1/*1. In those with the ALDH2*1/*2 genotype, the positive rate of facial flushing with one glass of beer was higher in those with ADH2*1/*2 and ADH2*2/*2 than those with ADH2*1/*1, although this was not significant. However, the ADH2 genotype did not seem to influence drinking frequency or amounts of alcohol intake in each ALDH2 genotype. CONCLUSIONS: This study finds further evidence for a contribution of the ADH2 polymorphism to skin reactions after either local or systemic ethanol exposure in Asian people. However, the effects of the ADH2 polymorphism may be mild because this polymorphism does not seem to influence alcohol drinking behavior in these study subjects.

Adult↗
PubMed2001

[A study on quantitative assessment of osteoporosis based on texture analysis].

A quantitative assessment on osteoporosis by using texture analysis has been developed on the basis of coherence mapping, two-dimensional power spectral estimation with modulated transfer function correction and fractal features analysis on trabecular pattern of bone X-ray images. Three objective features of trabecular pattern are selected to form a feature set that can be used in automatic classification for two grades of bone: normal bone and the bone with a medium degree of osteoporosis. This set of features is extracted from each of 16 sample bone X-ray images. With the use of Euclidean norm in the feature space, a center of cluster of each grade of bone is defined as the prototype. For each bone X-ray image, the distance from its features to the two prototypes in feature space is computed to provide an objective judgement on the degree of osteoporosis. The machine classification of 10 bone X-ray images based on the developed prototype is given, and the result indicates that the proposed method is potentially applicable to the objective diagnosis of osteoporosis.

Femur↗
PubMed2001

[Clinical analysis of intracranial metastases in gestational trophoblastic tumour].

OBJECTIVE: To evaluate characteristics of patients with intracranial metastases of gestational trophoblastic tumour (GTT) and determine the prognostic factors and therapeutic modality. METHODS: We retrospectively reviewed the records of 814 GTT patients treated at Peking Union Medical College Hospital from 1984 to 1998. Of them, 382 were choriocarcinoma and 61 developed brain metastases (16.0%); 432 were invasive mole and 8 of them presented brain metastases (1.9%). Patients with brain metastases were divided into three categories: Group A, individuals with no prior chemotherapy (30 cases); Group B, patients who had received chemotherapy before transferred to our hospital (31 cases); Group C, individuals who developed brain metastases during therapy in our hospital (8 cases). Apart from 12 patients died before or during the first cycle of chemotherapy, the remaining 57 patients were treated with 5-FU combined chemotherapy or etopside, methotrexate, kengshengmycin, /vincristine, cyclophosphamide (EMA/CO) regimen, the number of courses varied from 3 to 17 cycles. The median number of chemotherapy for each patient was 8.2. Intrathecal methotrexate chemotherapy was utilized for all patients. Emergency surgical decompression was performed in 4 cases who had symptoms of highly increased intracranial pressure. RESULTS: Apart from 12 patients died before they received regular therapy in our hospital, remission rate of other 57 patients was 71.9%. The cumulative survival rate for these 57 patients at 5 years was 45.8%. Women with no prior chemotherapy (group A) had outcomes significantly better than those who had been treated before transfer to our hospital (group B) and there were no survivors among the patients who developed brain metastases during active chemotherapy (group C) [P < 0.05 (A Vs B); P < 0.01 (A or B Vs C)]. CONCLUSIONS: Multiagent systemic chemotherapy combined with intrathecal methotrexate chemotherapy still play the key role in the management of brain metastatic GTT patients; Surgical decompression should be performed if significant neurologic symptoms are present.

Adult↗
PubMed2001

Eye irritation caused by formaldehyde as an indoor air pollution--a controlled human exposure experiment.

OBJECTIVE: The present study focuses on health assessment of wood based panels which are widely used in interior decoration practices over the recent years in China. Formaldehyde has been identified as chemical indicator of (IAO) and an indoor air pollutant. To test its health effects experiment was undertaken. METHOD: A small environmental test chamber (60/L) was used as the generator of emission gas from new panels, and was operating at a temperature of 22.7 +/- 0.6 degrees C and a humidity of 44.4 +/- 2.5% with an air exchange rate of 1.0 +/- 0.15 h-1. On the three experimental days the values of product loading in chamber were 4, 2 and 6 m2/m3, respectively. Eight people were selected randomly from the students and employees of Wuhan Health and Anti-epidemic Station as subjects, with an average age of 21.9 +/- 5.9 years, and a gender ratio of 1:1, and two of them were smokers (one male and one female). The subjects' eyes were exposed to formaldehyde through a pair of goggles. Each goggle had its flow inlet and outlet, and connected to chamber exhaust of emission gas and to an exhaust from the room. The exposure time was very short, just 5 minutes and the formaldehyde doses were at 1.65 +/- 0.01, 2.99 +/- 0.07 and 4.31 +/- 0.02 ppm. A 60-mm linear visual analogue rating scales was used to measure the intensity of sensory eye irritation and a video tape recorder was used to record eye blinking frequency. RESULTS: The results demonstrated that tests of sensory eye irritation and eye blinking can be used for materials testing, and that a dose-effect as well as a time-variance of the effect can be measured. CONCLUSION: The tests showed that eye irritation was perceived at all of the three levels.

Adolescent↗
PubMed2001

Nationality differences in distributions of serum lipids, lipoproteins and apolipoproteins levels in Xinjiang China.

OBJECTIVE: To reveal the distribution characteristics of serum lipids, lipoproteins, and apolipoproteins levels in different nationalities. METHODS: Quantitative levels of those traits mentioned above were determined and body height (H), weight (W) and body mass index (BMI = W/H2) were assessed in 773 Kazaks (men 360 and women 413) and 911 Han nationality (men 466 and women 445) from the Xinjiang Autonomous Region of China. RESULTS: Kazaks men and women, respectively, had significantly higher serum levels of HDL-c and ApoA1 (all P < 0.001) and significantly lower serum levels of TG (men P < 0.01, women P < 0.001), Lp(a) (all P < 0.005), ApoB (men P < 0.005, women P < 0.001) and ApoB/A1 (men P < 0.01, women P < 0.001) than their Han nationality controls. The threshold points of ApoA1 (< 1.2 g/L) and ApoB (> 1.2 g/L) are higher in Hans than in Kazaks for ApoA1 (20.7 vs 9.8%) and ApoB (18.6 vs 14.3%); the serum levels of TG, HDL-c, Lp(a), ApoA1 and ApoB/A1 are all closely correlated with nationality (P < 0.001, P = 0.001, and P < 0.05, respectively). CONCLUSION: The results suggest that the nationality differences exist in serum levels of lipids, lipoproteins, and apolipoproteins, and Kazaks have a superior serum lipid pedigree to Hans. This differences may come from genetic differences, which affect the serum levels of lipids, lipoproteins, and apolipoproteins by controlling lipid metabolism patterns. Future study will be needed to dissect to the role of genetic factors on serum lipids.

Apolipoproteins↗
PubMed2001

[Species composition and diversity of soil mesofauna in the 'Holy Hills' fragmentary tropical rain forest of Xishuangbanna, China].

The species composition and diversity of soil mesofauna were examined in fragmented dry tropical seasonal rainforest of tow 'Holy Hills' of Dai nationality, compared with the continuous moist tropical seasonal rain forest of Nature Reserve in Xishuangbanna area. 5 sample quadrats were selected along the diagonal of 20 m x 20 m sampling plot, and the samples of litterfall and 0-3 cm soil were collected from each 50 cm x 10 cm sample quadrat. Animals in soil sample were collected by using dry-funnel(Tullgren's), were identified to their groups according to the order. The H' index, D.G index and the pattern of relative abundance of species were used to compare the diversity of soil mesofauna. The results showed that the disturbance of vegetation and soil resulted by tropical rainforest fragmentation was the major factor affecting the diversity of soil mesofauna. Because the fragmented forest was intruded by some pioneer tree species and the "dry and warm" effect operated, this forest had more litterfall on the floor and more humus in the soil than the continuous moist rain forest. The soil condition with more soil organic matter, total N and P, higher pH value and lower soil bulk density became more favorable to the soil mesofauna. Therefore, the species richness, abundance and diversity of soil mesofauna in fragmented forests were higher than those in continuous forest, but the similarity of species composition in fragmented forest to the continuous forest was minimal. Soil mesofauna diversity in fragmented forests did not change with decreasing fragmented area, indicating that there was no species-area effect operation in this forest. The pattern of relative abundance of species in these forest soils was logarithmic series distribution.

Animals↗
PubMed2001

[Effect of HCO3- on root growth and nutrient absorption of different rice genotypes].

Solution culture experiments with Zn-inefficient cultivar (IR26) and the Zn-efficient cultivar (IR8192-31-2) were conducted to study the effect of HCO3- on the root growth and nutrient absorption of different rice cultivars. The results showed that HCO3- strongly inhibited the root growth of Zn-inefficient cultivar especially at low Zn concentration. In contrast, that of Zn-efficient cultivar was slightly stimulated by HCO3- at low Zn concentration. HCO3- not only inhibited Zn absorption of Zn-inefficient cultivar, but also inhibited its absorption of Fe, Mn, Cu, implying that there was no specific inhibition of HCO3- on Zn absorption. These results demonstrated that the inhibition of root growth by HCO3- was likely to be the initial action of HCO3- in inducing Zn deficiency in lowland rice. With treatment of bicarbonate, Zn concentrations in upper and lower leaves of the Zn-efficient cultivar and their ratios were higher than those of the Zn-inefficient cultivar. The results showed that the Zn-efficient cultivar could transport more zinc from lower leaves to upper leaves, which might be-one of mechanisms that the Zn-efficient cultivar adapted to zinc deficiency in calcareous soil.

Absorption↗
PubMed2001

[Cytomeglovirus infection after liver transplantation].

OBJECTIVE: To study the prophylaxis, diagnosis and treatment of cytomegalovirus (CMV) infection after liver transplantation. METHODS: The clinical data of 10 patients receiving liver transplantation from July 1999 to October 2000 in our hospital were retrospectively analyzed along with literature review. RESULTS: CMV infection occurred asymptomatic cally in 6 patients, of whom, 5 were cured. CONCLUSIONS: Fluorogenic quantitative PCR (FQ-PCR) is effective, sensitive and highly specific for detecting CMV infection. It is important to monitor the status of CMV infection in patients receiving liver transplantation preoperatively and postoperatively. The key point for prophylaxis and treatment of CMV infection is early prevention and treatment after operation. Ganciclovir can exert significant therapeutic effects on CMV infection.

Adult↗
PubMed2001

[Establishment of the drug resistant cell line of choriocarcinoma and the reversal of drug resistance by transfection of human interleukin 2 gene].

OBJECTIVE: To establish the drug-resistant cell line of choriocarcinoma and to study the transfection of the human interleukin 2 (hIL-2) gene into the established drug resistant cell line and investigate the reversal of the multidrug resistance. METHODS: The resistant cell line was established by pulse exposed choriocarcinoma cell line JEG-3 to etopside (VP-16) for ten months. The recombinant plasmid containing pcDNA3.1(+)-hIL-2 gene was constructed. The drug resistant cell line was transfected with the constructed plasmid by lipofectin, and the tumor cell colonies containing the IL-2 sequence were selected by genetin. The expression of hIL-2 and drug resistant-related genes was detected by reverse transcript polymerase chain reaction. The chemosensitivity of the gene-transfected tumor cells and the non transfected cell lines to methetraxate, VP-16, kengshengmycine, paclitaxol and 5-fluorouracil was determined by the methyl thiazolyl tetrazolium cytotoxicity assay. RESULTS: The transfected cells expressed human hIL-2 gene, and showed the reversal of multidrug resistance by methyl thiazolyl tetrazolium assay. The transfected cells expressed no multidrug resistance gene-1 (MDR1) on mRNA level. Drug resistance index to VP-16 decreased from 38.7 to 6.0 and 6.1, the index to methetraxate decreased from 14.5 to 2.6 and 2.5, to methetraxate from 13.0 to 2.0. CONCLUSION: The transfection of hIL-2 gene into the drug resistance cell line of choriocarcinoma can modulate the MDR1 expression on the mRNA level, and reverse the drug resistance.

Choriocarcinoma↗
PubMed2001

[Presynaptic 5-hydroxytryptamine receptors enhance cholinergic neurosecretion in the human iris-ciliary body].

OBJECTIVE: To characterize presynaptic 5-hydroxytryptamine (5-HT) heteroreceptors which modulate 3H-acetylcholine (3H-ACh) release in isolated human iris-ciliary bodies (ICBs). METHODS: ICB tissue segments were perfused and incubated with 3H-choline, and electrically stimulated four times (S1, S2, S3 and S4) at 10 Hz for 1 min to elicit 3H-ACh secretion. Test agents, 5-HT agonists and antagonists, were added before S2, S3 and S4 and their effects were determined by the stimulation ratio (Sx/S1) of evoked 3H-ACh release. 3H-ACh in perfusate fractions was isolated by ion exchange chromatography and analyzed for radioactivity by liquid scintillation spectrometry. RESULTS: The evoked 3H-ACh release was enhanced in a concentration-dependent manner by 5-HT [10(-9)-10(-5) mol/L, 50% effective concentration values (EC50) = 3.36 x 10(-8) mol/L] as well as 5-methoaxytryptamine [5-MOT (agonist), 10(-8)-10(-5) mol/L, EC50 = 6.59 x 10(-7) mol/L]. The selective 5-HT4 antagonist, GR113808A (10(-8) mol/L) inhibited the 5-HT-inducing increase of the cholinergic response, producing parallel right shifts of the 5-HT concentration-response curves (t = 4.012, P < 0.01). The selective 5-HT3 antagonist ondersetron (5 x 10(-7) mol/L) and tropisetron (10(-9) mol/L) did not affect 5-HT inducing 3H-Ach release (t = 2.215, P > 0.05). CONCLUSION: Our results indicate that cholinergic terminals in the human ICB contain 5-HT heteroreceptors that may promote the release of 5-HT and belong to the 5-HT4 subtype.

5-Methoxytryptamine↗
PubMed2001

Gene-CYP11B2 expression in rat liver in hepatic fibrogenesis induced by CCl4.

OBJECTIVE: To identify aldosterone synthase gene-CYP11B2 mRNA expression in normal and fibrotic liver in rats and evaluate the curative effect of antisterone. METHODS: 160 Wistar rats weighing about 250 g were divided into 4 groups. In the model group (n = 40), the rats were injected with 40% CCl4 (0.25 ml/100 g) subcutaneously three times a week. In the antisterone group (n = 40), the rats were injected with 40% CCl4 (0.25 ml/100 g) subcutaneously three times a week. Antisterone equivalent to 20 mg.kg-1.d-1 was given intragastrically (ig). In the malotilate group (n = 40), the rats were injected with 40% CCl4 (0.25 ml/100 g) subcutaneously three times a week. Malotilate equivalent to 50 mg/kg-1.d-1 was given ig. In the control group (n = 40), the rats were injected with olive oil only. After 2, 4, 6, 8 and 10 weeks, animals were sacrificed, and morphological examination was carried out. The area of collagen was examined with an Image Analyse System. Expression of the aldosterone synthase gene, CYP11B2 mRNA, in fibrotic and normal liver was detected by means of reverse transcriptase polymerase chain reaction (RT-PCR) and in situ hybridization. RESULTS: In situ hybridization and RT-PCR showed that the expression of CYP11B2 mRNA, which localized in the endoplasm of hepatic stellate cells (HSCs), was up regulated when fibrogenesis occurred. Histological observation indicated that the grade of fibrosis and the area of collagen in the antisterone group were less than those in model group before 6 weeks (P < 0.05). There was no significant difference between the antisterone and malotilate groups (P > 0.05). After that, however, the grade of fibrosis and the area of collagen in the antisterone group were higher than those in the malotilate group (P < 0.05). There was no significant difference between the antisterone and model groups (P > 0.05). CONCLUSIONS: The expression of CYP11B2 mRNA is up regulated in fibrotic liver. Antisterone can have a partial fibrogenesis-inhibiting effect in the early stages.

Animals↗
PubMed2001

ATP sensitive K+ channel may be involved in the protective effects of preconditioning in isolated guinea pig cardiomyocytes.

OBJECTIVE: To develop a cellular model of preconditioning by a brief period of hypoxia in isolated guinea pig cardiomyocytes and to determine whether or not an ATP sensitive K+ (KATP) channel is involved in ischemic preconditioning. METHODS: Single myocytes were isolated from the ventricle of adult guinea pigs. The experimental chamber allowed the cells to be exposed to low O2 pressure. During hypoxic preconditioning, the cells were equilibrated with normaxic solution for 10 minutes and then exposed to hypoxia for 5 minutes, followed by 10 minutes of reoxygenation. The cells were then subjected to 20-180 minutes of hypoxia and reoxygenation. Ionic currents were studied with the patch clamp technique in whole-cell and cell-attached configurations. RESULTS: A 5-minute hypoxic preconditioning offered a significant protection from cell injury in subsequent hypoxia-reoxygenation. After a latency of more than 15 minutes, hypoxia induced a time-independent outward K+ current which could be blocked by 5 mumol/L glibenclamide. At 10 mV, the current increased from 78 +/- 15 pA to 1581 +/- 153 pA (P < 0.01, n = 18). However, the latency to develop KATP channel currents (IKATP) was greatly shortened in preconditioned cells, and the current was increased acceleratively. At 10 mV, the current more than 4 nA was recorded in preconditioning cells. In the single channel recordings, the time interval from the first channel opening to maximum opening was also markedly abbreviated in preconditioned cells. CONCLUSION: Isolated guinea pig cardiomyocytes can be preconditioned with a brief period of hypoxia. This hypoxic preconditioning may modify the KATP channel, and make the channel open more readily during the second hypoxia.

Adenosine Triphosphate↗
PubMed2001

Reversing drug resistance in the ovarian carcinoma cell line SKOV3/mdr1 in vitro by antisense oligodeoxynucleotides.

OBJECTIVE: To investigate the effect of multidrug resistance gene 1 (mdr1) antisense oligodeoxynucleotides (ODNs) on reversing multidrug resistance in the drug resistant ovarian carcinoma cell line SKOV3/mdr1. METHODS: The ovarian carcinoma cell line SKOV3 transducted with a human multidrug resistance gene (mdr1) served as the drug resistant model (SKOV3/mdr1). The mdr1 antisense ODNs was transfected into SKOV3/mdr1 cells while mediated by lipofectamine. Reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the expression and the amount of the mdr1 mRNA in the cells. The positive rate and function of the mdr1 gene product P-glycoprotein (Pgp) in the mdr1 antisense ODNs treated SKOV3/mdr1 cells were determined by flow cytometry and rhodamine 123 efflux. Drug resistance in the SKOV3/mdr1 cell line was observed by MTT assay and cell colony culture. RESULTS: The mdr1 mRNA level was decreased to about 60% of that of beta-actin after mdr1 antisense ODNs treatment. The Pgp positive rate of mdr1 antisense ODNs treated SKOV3/mdr1 cells decreased from 100% to 52.6% (P < 0.01). The intracellular rhodamine 123 retention was increased from 9.1% to 33.8% (P < 0.01). The chemoresistance to taxol decreased to 58% of SKOV3/mdr1 with mdr1 antisense ODN treatment. Compared with SKOV3/mdr1 cells in the control group, under a certain range of drug concentrations, the number of drug resistance colonies in mdr1 antisense ODNs treated SKOV3/mdr1 cells for taxol and doxorubicin decreased by 8.6 +/- 0.8 fold and 3.1 +/- 0.6 fold, respectively. Some non-specific functions during oligodeoxyncleotide treatment was also detected. CONCLUSION: mdr1 expression in the SKOV1/mdr1 cell line was partially inhibited after mdr1 antisense ODNs treatment at the mRNA and protein level, increasing the chemotherapy sensitivity of this drug resistant ovarian carcinoma cell line.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed2001

Gene expression of glucose transporters and its regulation by glucose in mesothelial cells.

OBJECTIVE: To observe the influence of glucose on the expression of glucose transporters (GluTs) in peritoneal tissues. METHODS: Mesothelial cells (MsCs) from Sprague-Dawley (SD) rats were cultured in medium with glucose 214.4 mmol/L or 75.5 mmol/L. The normal medium with glucose 17.5 mmol/L was used as control. Total RNA was extracted from each sample after 24 hours incubation. Reverse transcript polymerase chain reaction (RT-PCR) was performed with primers corresponding to sodium-glucose transporter (SGIT1) and GluT1-GluT4. mRNA expression of the above GluTs from each sample was measured with quantitative PCR. RESULTS: GluT1 and GluT2 mRNA can be detected in MsCs from SD rats, while no positive bands can be found specifically for GluT3, GluT4 and SGiT1. Quantitating the amount of PCR products indicated that the levels of GluT1 mRNA in MsCs cultured 24 h in both 214.4 mmol/L glucose and 75.5 mmol/L glucose medium decreased dramatically compared with that in normal medium (P < or = 0.01). While under the same conditions, the levels of GluT2 mRNA in MsCs cultured 24 h in 214.4 mmol/L and 75.5 mmol/L glucose medium both increased significantly (P < 0.01). CONCLUSIONS: GluT1 is strongly expressed in MsCs under normal glucose levels and decreased dramatically under high glucose conditions, while GluT2 expressed at a low level in normal medium and increased greatly after incubation in high glucose conditions. This may play a great role in glucose absorption during peritoneal dialysis and have some connection with ultrafiltration failure due to the alteration of glucose absorption after long-term dialysis.

Animals↗