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Biomedical subjects

X Y Lu

Publications and source records attributed to X Y Lu.

53 records · Page 3Linked to original sources
PubMed1990

[Studies on elimination of mycoplasmas from cell line cultures].

Mycoplasmas were effectively eliminated from contaminated LTK and CHO cell lines by means of the antibiotic Tiamutin (10 micrograms/ml), while Kanamycin and Gentamycin were less potential. An elimination procedure which involved the consecutive treatment of the cell cloning over a periods of 21-28 days was adopted. This procedure was effective when applied to cell lines contaminated with unidentified and partially non-cultivable strains of mycoplasmas which could be detected by electronic microscope.

Animals↗
PubMed1988

The interaction between native serum albumin and hepatitis B virus.

Purified hepatitis B virus particles were obtained from HBeAg positive serum by sucrose gradient ultracentrifugation and sephadex G-200 gel filtration. These virions formed a precipitation line in counterimmune electrophoresis with anti-albumin antibody, but the reaction could be inhibited by anti-HBs. After two months at 4 degrees C, another precipitating line was formed under the same condition which could not be inhibited by anti-HBs and was, thus, due to free albumin. When that sample was incubated at 37 degrees C overnight, the line of free albumin disappeared. The virion bound albumin was monomeric in non-denaturing gel electrophoresis. These results suggest that a reversible binding between virion and albumin may occur in vivo and does not require chemical modification or cross-linking.

Cold Temperature↗
PubMed1988

Study of descending inhibitory and facilitory effect of the orbital cortex on the electrical discharges of medial geniculate body (MGB) neurons in rabbits.

Experiments were performed on 16 rabbits immobilized with flaxedil. The recording of spontaneous discharges and the discharges of single MGB neurons caused by clicks showed that when the cerebral-orbital cortex was stimulated, of 92 MGB neurons responding to clicks, 25 units (27.2%) were inhibited, 14 units (15.2%) were facilitated, and 15 units with spontaneous discharges were completely inhibited. The topographical distribution of stimulated points with such inhibitory and facilitory effect is quite concentrated on the orbital cortex. Stimulation of two adjacent points of the orbital cortex could respectively result in different kinds of inhibitory and facilitory effect on discharges of a same MGB neuron. Due to the existence of this descending effect with a short latent period (2-3 ms), the authors have reason to believe that there might be an "orbital cortex-MGB" direct pathway. Moreover, application of 2% procaine to block the auditory cortex behind the rhinal sulcus (ACBRS) could abolish the facilitory effect, with a long latency, of the orbital cortex. Therefore, the influence of the orbital cortex on MGB might be also transmitted via the "orbital-ACBRS-MGB" circuit.

Animals↗
PubMed1983

Study of inhibitory effect of amygdaloid stimulation on auditory response of medial geniculate body (MGB) and analysis of transmissive pathway of the said effect.

Experiments performed on 103 rabbits immobilized with Flaxedil, and the recording of the electrical discharges of single MGB neuron caused by clicks, burst or sustained tone showed marked inhibition of the reaction of 47 out of 75 (62.7%) MGB units responding to sound stimuli, when the amygdaloid complex was stimulated. The latent period of such an inhibitory effect was 5-12 msec and its duration about 2-13 msec. Evoked responses with the latency of 1-2 msec for the stimulation of medial, central and lateral nuclei of amygdala could be recorded from the temporal and parietal cortex. The largest response was recorded from Woolsey's AI area of the temporal cortex. Stimulation of the Woolsey's AI auditory area could also result in a distinct inhibition of the spontaneous as well as the evoked discharges of MGB neurons in response to sound stimulation. Moreover, application of 1% procaine to the surface of auditory cortex could block the inhibitory effect of the amygdala on the MGB neurons. Based on the facts mentioned above, the authors have reason to believe that the inhibitory effect of the amygdaloid complex on the activity of MGB units might be transmitted via the amygdala-auditory cortex-MGB circuit. The biological significance of such an suppressive effect has been discussed.

Acoustic Stimulation↗