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Biomedical subjects

X Xu

Publications and source records attributed to X Xu.

At least 397 records · Page 22Linked to original sources
PubMed2000

[Antiphospholipid thrombosis syndrome and acquired activated protein C resistance].

OBJECTIVE: To explore the correlation between antiphospholipid antibodies (APA) activated protein C resistance (APCR) and antiphospholipid thrombosis (APL-T) syndrome and investigate the mechanism of thrombosis in APL-T syndrome. METHODS: ELISA, APTT-LA and APTT+/-APC methods were used to detect anticardiolipin antibodies (ACA), lupus anticoagulants (LA) and APCR in 20 APL-T syndrome patients respectively. RESULTS: Twenty patients were diagnosed as APL-T. The patients were classified in three ways. According to etiology, 14 of 100 systemic lupus erythematosus (SLE) patients were diagnosed as "secondary" APL-T, and 6 of 16 "unexplained" thrombosis and spontaneous abortion as "Primary" APL-T syndrome. According to antibodies, 12 of 20 patients with positive LA were diagnosed as lupus anticoagulant thrombosis syndrome (LA-T), 12 of 20 patients with positive ACA as anticardiolipin antibodies thrombosis syndrome (ACA-T). According to clinical presentation: 7 of 20 patients with deep vein thrombosis were classified as type I APL-T, 3 of 20 patients with cerebrovascular thrombosis as type II, 1 of 20 patients with vein cerebrovascular thrombosis and spontaneous abortion as type IV (mixing), and 9 of 20 patients with spontaneous abortion as type V (fetal wastage type). The ACAs and LA are strongly associated with APL-T. There is close relationship between APCR and APL-T. CONCLUSION: The acquired APCR might be an important reason for thrombosis in APL-T caused by APA and LA.

Activated Protein C Resistance↗
PubMed2000

[Rapid detection of alpha-thalassemia of Southeast Asian deletion by polymerase chain reaction and its application to prenatal diagnosis].

OBJECTIVE: To establish a rapid and simple polymerase chain reaction (PCR) method for detecting alpha-thalassemia of Southeast Asia deletion, and apply it to the prenatal diagnosis for high risk fetuses. METHODS: Two pairs of primers were designed: one pair bridging the breakpoints to identify the specific deletion, the other located in the common deletion region of --(SEA), -alpha(3.7) and -alpha(4.2) gene to detect the normal chromosomes. In this system, the two amplifications ran in the same PCR tube under identical condition. RESULTS: A 740 bp fragment was amplified in chromosomes with --(SEA) determinant and a 1,052 bp fragment in normal chromosomes. For prenatal diagnosis, 3 of 8 at-risk cases were diagnosed as normal, 3 as heterozygotes, and 2 as homozygotes of --(SEA) deletion. CONCLUSION: This detection method is rapid and accurate and can be used as a routine method for carrier detection and prenatal diagnosis.

Female↗
PubMed2000

[The toxicity of busulphan and cyclophosphamide as the preparative regimen for allogeneic peripheral blood stem cell transplantation].

OBJECTIVE: To analyse the treatment-related complications of busulphan and cyclophosphamide (BU-CY) as the conditioning regimen for allogeneic peripheral blood stem cell transplantation (allo-PBSCT). METHODS: The clinical data of 40 leukemia patients undergoing allo-PBSCT between June 1997 and May 1999 in our BMT center were retrospectively analysed. RESULTS: Recovery of neutrophil and platelet was achieved at a median of day +13 (9 - 28) and day +12 (7 - 60) respectively. Acute GVHD occurred in 17 of 40 patients (42.5%) with grade II-IV in 10 patients (25%). Chronic GVHD developed in 21 out of 30 evaluable patients (70%). Mild to severe mucositis occurred in 30 patients (75%), and 4 of them had severe esophagitis with bleeding. Haemorrhagic cystitis developed in 8/40 (20%) patients, the median time of its onset was day +100 (+7 to +165). Six of 40 patients (15%) developed interstitial pneumonia (IP), 5 of them were due to cytomegalovirus infection, and the remaining one due to pneumocystis carinii infection. No hepatic veno-occlusive disease was observed and no seizure occurred. During the median follow-up of 480 (300 - 1000) days, 4 (10%) patients relapsed and 8 (20%) patients died of the transplant-related complications. The 3 year leukemia-free survival rate was 70%. CONCLUSION: BU (domestic busulfan)-CY regimen is relatively easy to administer and well tolerated, with low extramedullary toxicities.

Acute Disease↗
PubMed2000

[Detection of soluble endothelial protein C receptor (sEPCR) in patients with CHD, DM and SLE].

OBJECTIVE: To evaluate the clinical significance of plasma soluble endothelial protein C receptor (sEPCR) level in patients with coronary heart disease (CHD), diabetes mellitus (DM) and systemic lupus erythematosus (SLE). METHODS: ELISA of antibody-sandwiched principle was used to detect sEPCR in 34 patients with CHD, 42 with DM-II, 63 with SLE and 20 normal controls (NC). RESULTS: The plasma level of sEPCR in patients with DM was higher than that in NC group (P < 0.05). The sEPCR level in SLE patients with thrombosis and non-thrombosis are higher than that in NC group (P < 0.005 and P < 0.001, respectively). There is no significant difference between the two groups of SLE (P > 0.05), and between the CHD and NC groups. CONCLUSION: sEPCR level increased in DM and SLE, it seems related to the process of inflammatory reaction and damage of endothelial cell.

Adolescent↗
PubMed2000

[Expression of ET of early ischemic myocardium in rats].

To study the expression of ET of early ischemic myocardium, we designed a model of early myocardial ischemia (EMI) in rats. The study was conducted with SABC method and HE staining. Cardiac myocytes of ischemia showed a little ET-positive at 60-min. Cardiac myocytes, endothelium and smooth muscle of vessel showed ET-positive staining in the area of EMI after 120-min. Staining was negative for Ischemia myocardium at 30-min and for the control group. No change was seen with HE staining in all groups. The data indicates that ET-SABC method may reveal the injury of myocardial ischemia 60-min and longer and may provide a new marker to diagnose EMI in forensic pathology.

Animals↗
PubMed2000

[Expression and implication of c-myb and bcl-2 proteins in C6 gliomas].

This study was aimed at the level and implication of expression of c-myb and bcl-2 proteins in C6 glioma. The therapeutic effect of oncogene c-myb antisense oligodeoxynucleotide on C6 glioma in nude mice was studied. C6 glioma cells were implanted into the cutis of 36 nude mice. Ten days later, the nude mice were randomly divided into three groups and were treated with SON, AON and normal saline respectively. Three mice of every group were killed at 4 days, 8 days, 12 days and 16 days after treatment, respectively. The levels of c-myb and Bcl-2 proteins expression in the three groups were observed by the S-P immunohistochemical method. The results showed the expression of c-myb and bcl-2 proteins was significantly decreased in the AON group, compared with that in the SON and control groups (P < 0.05). These data suggest that c-myb and bcl-2 proteins may play an important role in malignant glioma, and c-myb gene and bcl-2 gene may be involved in the tumorigenesis and development of glioma.

Animals↗
PubMed2000

[The influence of external stimulation on content and quality of volatile oil in Lignun Santali albi].

The authors analyzed the quality of Ligmum Santali Albi formed by the external stimulation of hormone and windburn by GC-MS-DS. The results showed that the content of volatile oil is 2.34% in the heart wood formed in 10 years tree age of Santalum album (SA) after 2 years stimulation continuously with a definite concentration of hormone, which is near to the 25 years tree age of SA in the same place. The GC-MS analysis showed that the content of santalol and other chemical components in volatile oil are similar to the 25 years tree age of SA. It is indicated that a definite concentration of hormone stimulated the SA may shorten the formation of the heart wood. The heart wood can be also formed by the broken branches after 2 years windburn, but its content of volatile oil is only 1/2 of the heart wood formed by hormone stimulation.

Age Factors↗
PubMed2000

[Simultaneously corneal laceration repair, traumatic cataract removal and intraocular lens implantation].

OBJECTIVE: The study aimed to evaluate the outcome of patients who undrwent simultaneously corneal laceration repair, cataract extraction and posterior chamber lens implantation. METHODS: Traumatic cataract extraction and posterior chamber intraocular lens (PC IOL) implantation were performed in 36 eyes of 36 cases with corneal laceration. YAG laser was the postoperative procedure for embrane before the lens and after cataract. Penetrating keratoplasty was performed for central cicatrice of cornea. All the patients have been followed up for 3-12 months. RESULTS: The postoperative visual acuity s.c. was 0.1-0.3 in 8 cases(22.8%), 0.4-0.5 in 14 cases (40%) 0.6-0.8 in 13 cases (37.1%) on following up. There were no serious complication attributable to PC IOL implantation. CONCLUSION: As long as the range of surgical indication was known well, PC IOL implantation can get good clinical effects at the time of primary repair.

Adolescent↗
PubMed2000

[Assay and L1 gene sequence analysis of human papillomavirus type 6 and 11 in condylomata acuminata].

OBJECTIVE: To determinate the prevalence of HPV type 6, 11 in condylomata acuminata, and analyze the sequence of L1 gene and its deduced L1 protein. METHODS: PCR was used to amplify DNA sequences between E6 and E7 of HPV type 6 and 11. The PCR products were identified by the fragment length cleaved with restriction enzyme RsaI. L1 genes were amplified with high fidelity Taq, and cloned DNA sequences were detected by the dideoxynucleotide method. RESULTS: In 34 examples of condylomata acuminata, HPV11 was found in 25 examples (73.5%), HPV6 in 15(44.1%), including HPV6 + HPV11 in 6(17.6%). In comparison with the prototypes there were 7-8 point mutations in HPV11 L1 gene and 3 in HPV6L1 gene. L1 encoded protein changes showed up only in 1-3 amino acids in the former cases. CONCLUSION: Most of patients tested with condylomata acuminata are mainly infected by HPV11, the rest of them by HPV6 or by co-infection of two mixex types. Sequence analysis of L1 genes indicated that mutations were found in both HPV6 and 11, whereas deduced amino acid mutations in L1 protein were limited in HPV11 only.

Adult↗
PubMed2000

[The diagnostic and prognostic values of assay of serum vascular endothelial growth factor in epithelial ovarian cancer].

OBJECTIVE: To detect the level of the preoperative serum vascular endothelial growth factor (VEGF) in patients with epithelial ovarian cancer, and to evaluate the correlation between preoperative serum VEGF level and prognosis of epithelial ovarian cancer. METHODS: Forty-one patients with epithelial ovarian cancer (EOC), 10 patients with borderline ovarian tumor (BOT), and 25 women with benign ovarian tumor (BET) were included as three study groups, while 20 healthy women were selected as a control group. Enzyme-linked immunosorbent assay (ELISA) was utilized to measure the serum VEGF level. RESULTS: The serum VEGF levels in patients with EOC were higher than those in patients with BOT, or with BET, or those in healthy women. The median and ranges of serum VEGF levels were as follows: EOC: median 415.0 pg/ml, range 110-2,120 pg/ml; BOT: median 312.5 pg/ml, range 100-1,250 pg/ml; BET: median 170 pg/ml, range 70-925 pg/ml; healthy women: median 165 pg/ml, range 100-735 pg/ml. When median preoperative serum VEGF levels were grouped by tumor stages, histological grade of tumor cells, histological type of the tumor, lymph node involvement, and omentum involvement, significant correlations between serum levels of VEGF and histological grade, lymph node involvement and omentum involvement were found. The patients with high serum VEGF appeared to be accompanied with poor 3-year survival. CONCLUSIONS: Preoperative serum VEGF levels seemed to be correlated with the malignant behavior of ovarian cancer, which might provide some prognostic value.

Adult↗
PubMed2000

[Changes of handling of calcium in mesenteric vascular smooth muscle cells of calcium overload rat].

OBJECTIVE: To investigate if there existed abnormal handling of calcium in vascular smooth muscle cells (VSMC) in calcium overload rat (CaOMV). METHODS: Cytosolic and nuclear calcium ion handling in mesenteric VSMC both in calcium overload rat and in Wistar rat were studied by using laser scanning confocal microscopy together with calcium-sensitive-dye Fluo-3/AM. RESULTS: The results showed that both nuclear and cytosolic calcium level ([Ca2+]n, [Ca2+]i) in mesenteric VSMC in calcium overload rat under resting condition was not higher than that in normal rat. However, [Ca2+]n and [Ca2+]i in CaOMV increased more significantly than that in normal rat when induced by drugs such as KCl, Bay-k8644, Ang-II, IP3, CHCl3, and CPA. CONCLUSIONS: (1) There exists abnormal handling of calcium in CaOMV because of it's overreaction to stimulators, which indicate that intracellular and extracellular calcium overload coexist in age-related arteriosclerosis; (2) Not only Ca2+ gating system of plasma membrane but also calcium pool in CaOMV are irregular; (3) Cytosolic calcium as well as nuclear calcium are handled improperly in CaOMV.

Animals↗
PubMed2000

[A quantum chemical study of pi-back-donation bond and Raman intensity of 1 sigma + electronic state of Pt-CO molecule].

The Raman spectroscopic properties of Pt-CO molecule have been investigated based on the electronic state 1 sigma + determined by the HF and B3LYP methods. The result shows that the calculated stretching vibrational frequencies of the Pt-C and C-O bonds depend on the method and the basis sets used. It indicates that it is important to adopt an appropriate method to describe pi-donation and pi-back-donation bond. The result of the differential Raman scattering cross section for the stretching vibrations of the Pt-C and C-O bond shows that the latter is significantly larger value compared to the former.

Adsorption↗
PubMed2000

[Conformity of cancerous diagnosis of serum-fluorimetry under the influence of carotenoid fluorescence].

We observed that carotenoid had sensitization for fluorescence of porphyrin as cancerous diagnostic technology was applied in clinic by serum-fluorimetry. The Forster theory was used in order to analyse sensitized fluorescence between carotenoid and porphyrin in this paper. Transfer characteristic between energy donor and energy acceptor was calculated and discussed its influence on cancerous diagnosis.

Biomarkers, Tumor↗
PubMed2000

[Raman spectra and IR spectra of 3-triphenylgermyl-1,1-diphenyl-1-butanol and its derivatives].

The Raman spectra and FTIR spectra of 3-triphenylgermyl-1,1-diphenyl-1-propanol (A), 3-triphenylgermyl-1,1-diphenyl-1-butanol (B) and 3-triphenylgermyl-2-methyl-1,1-diphenyl-1-propanol (C) have been measured and analyzed. In Raman spectra of these compounds, the Ge--Ph stretching vibrations are near 1,000 cm-1, and the Ge--C stretching vibrations are 594.1 (for A), 578.7 (for B), 599.2 cm-1 (for C). The spectra show that the three compounds have similar molecular vibration characteristics.

English Abstract↗
PubMed2000

[Study on the photoinduced-color changing about novel azobenzocrown ether derivatives 16ABC-OH and 16ABC-NH2 in water solution and Langmuir-Blodgett film].

Two kinds of azobenzocrown ether derivation 16ABC-OH and 16ABC-NH2 have been synthesized, and their photoinduced color changing phenomena has been investigated in water solution, the reaction of 16ABC-OH is the photochemical Cis-trans-isomerization, and it is a first-order reaction, but the reaction of 16ABC-NH2 photochemical Cis-trans-isomeriation is very complex; and it is not a first-order reaction. In Langmuir-Blodgett film of 16ABC-OH, there is not molecular self-assembly phenomenon, the part of Cis-16ABC-OH become to iso-16ABC-NH2, but in the film, 16ABC-NH2 form H-Polymer by self-assembly, and the photochromic Cis-trans-isomerization is forbidded.

English Abstract↗
PubMed2000

[Experimental study on using mGTR and silicone gel tube connection to repair facial nerve defect in rabbits].

OBJECTIVE: To observe and compare the effects of using mGTR or Silicone tube to repair the defect of facial nerve. METHODS: mGTR and Silicone tube methods were performed on three groups of rabbits. Then physical and histological examination was carried out to get the results of these two repair methods. RESULTS: Both the two methods can induce regeneration of facial nerve. The mGTR was more effective and faster in repairing the nerve defect. Three months after operation, the distance of NCV between the two sides of facial nerve in mGTR group was 2.33+/-1.2 m/s, 10.21+/-1.5 m/s in Silicone tube group, and 2.16+/-1.6 m/s in autogeneous nerve transplantation group. CONCLUSION: The mGTR is more effective than Silicone tube method in repair facial nerve. mGTR has some other advantages, it is likely to be a new method of nerve repair on clinical domain.

English Abstract↗
PubMed1999

Transcriptional regulation of Fas gene expression by GA-binding protein and AP-1 in T cell antigen receptor.CD3 complex-stimulated T cells.

Fas (CD95 or APO-1), a transmembrane cell surface receptor of the tumor necrosis factor receptor family, is up-regulated in activated T lymphocytes. Our present study identified an upstream enhancer element (between nucleotide positions -862 and -682) containing a GA-binding protein (GABP) site and a low affinity activating protein-1 (AP-1)-binding site. T cell activation increased the DNA binding of GABP and AP-1 to this enhancer site. The specificity of GABP and AP-1 binding was demonstrated by competition electrophoretic mobility shift assay and supershift electrophoretic mobility shift assay with antibodies against GABP and AP-1, respectively. Mutational analysis of Fas promoter revealed that both GABP- and AP-1-binding sites were required for initiating Fas gene transcription. We further show that anti-CD3 mAb, phorbol 12-myristate 13-acetate, and phorbol 12-myristate 13-acetate/ionomycin strongly activated promoters carrying multiple copies of the Fas enhancer, and mutation of either the GABP or AP-1 binding site severely reduced transcriptional activity. Taken together, these results suggest that the transcription factors GABP and AP-1 play a critical role in the induction of Fas gene expression in T cell antigen receptor.CD3-stimulated Jurkat cells.

Base Sequence↗