Biomedical subjects

X Xu

Publications and source records attributed to X Xu.

At least 253 records · Page 14Linked to original sources

PubMed2001

Family-based association test method: age of onset traits and covariates.

We apply different family-based association test (FBAT) statistics for age of onset traits to the Genetics Analysis Workshop 12, problem 2 data. To evaluate different FBAT statistics we used the software package FBAT, which allows one to evaluate any test statistic that can be expressed as the sum of products between an arbitrary function of an offspring's genotype with an arbitrary function of the offspring's phenotype even if there are missing parental information. For single nucleotide polymorphisms (SNPs) in gene 1, our age-of-onset FBAT test based on the exponential model is significantly more powerful than the test by Mokliatchouk et al. [Hum Hered 51:46-53, 2000], which is based on the Cox model. We suggest incorporating covariates into FBAT statistics by replacing the trait values by their regression residuals. For the age of onset trait statistics we find that deviance residuals have much more power than "plain" martingale residuals. We discuss why for SNPs in gene 1, the usual affectation status trait, which underlies the transmission disequilibrium test (TDT), has higher power than the age-of-onset trait. We find only weak evidence (p = 0.0002) that marker D06G032 is associated with the affectation status.

Adult↗

PubMed2001

The association between PPP1R3 gene polymorphisms and type 2 diabetes mellitus.

OBJECTIVE: To detect the relationship between the polymorphism of the glycogen-targeting regulatory subunit of the skeletal muscle glycogen-associated protein phosphatase 1 (PPP1R3) gene and type 2 diabetes by case-control study. METHODS: We genotyped the PPP1R3 gene Asp905Tyr polymorphism and a common 3'-untranslated region AT (AU)-rich element (ARE) polymorphism in 101 type 2 diabetic patients and 101 controls by oligonucleotide ligation assay (OLA) and polyacrylamide gel elecrophoresis, respectively. RESULTS: Subjects with Tyr/Tyr genotypes whose body mass index (BMI) < 25 were used as the reference group. Those whose BMI > or = 25 with Asp905 had a 3.66-fold increase (95% CI: 1.48-9.06, P = 0.005) in type 2 diabetes risk. No association was found between 3'UTR ARE polymorphism and type 2 diabetes mellitus (OR = 1.15; 95% CI: 0.62-2.14, P = 0.65). CONCLUSION: A joint effect between the Asp905 and BMI increases the risk of type 2 diabetes, and Asp905Tyr and ARE polymorphism of PPP1R3 gene are not the major diabetogenic gene variants in Chinese population.

AT Rich Sequence↗

PubMed2001

[Allogeneic peripheral blood stem cell transplantation for treatment of leukemia].

OBJECTIVE: To observe engraftment kinetics, incidence and severity of graft-versus-host disease (GVHD) and clinical outcome in 40 recipients undergoing allogeneic peripheral blood stem cell transplantation (allo-PBSCT). METHODS: 40 leukemia patients with a median age of 35 years underwent allo-PBSCT from June 1997 to May 1999. PBSC were mobilized with G-CSF at a dose of 5 microg/kg S. C. every 12 hours for 5 days. A median of 7.7 (range 2.0 to 16.8) x 10(6) CD(34)(+) cells/kg was infused into the recipients. Busulfan (BU) and cyclophosphamide (CY) were used as conditioning regimen. All patients received cyclosporine A and either methotrexate (n = 34) or methylprednisolone (n = 6) for GVHD prophylaxis. RESULTS: Engraftment of neutrophils and platelets was achieved at a median of day + 13 (9 - 28) and day +12 (7 - 60) respectively. Patients receiving >or=4 x 10(6) CD(34)(+) cells/kg or given G-CSF post transplant had significantly accelerated neutrophil and platelet engraftment. Acute GVHD occurred in 17 of the 40 patients (42.5%), with grade II-IV acute GVHD in 10 patients (25.0%); chronic GVHD developed in 21 out of the 30 evaluable patients (70.0%) with a median follow up of 480 days (range 300 - 1,000). The transplant related mortality is 20.0% and the relapse rate is 10.0%. The probability of leukemia free survival at 3 years is 70.0%. CONCLUSIONS: These results indicate that allo-PBSCT can provide rapid hematopoietic reconstitution without an increased incidence of acute GVHD, but may be associated with high risk of chronic GVHD.

Adult↗

PubMed2001

[The natural history of electrophysiological and magnetic resonance changes of subacute combined degeneration].

OBJECTIVE: To observe the presentations and in the patients with subacute combined degeneration (SCD) changes of electrophysiology (EP) and magnetic resonance image (MRI) before and after treatment and to analyze their values in diagnosis. METHODS: 10 patients received a series of examinations, including clinical neurological functions, serum vitamin B12 and folic acid concentrations, spinal cord MRI, peripheral nerve conduction velocity, sensory evoked potential (SEP) and visual evoked potential(VEP). They were followed up for 3 to 36 months. The changes of spinal cord MRI and SEP before and after the treatment were studied. RESULTS: After Vitamin B12 therapy, all the patients improved,but there were still some abnormalities of SEP left. The lesions could be shown sensitively by EP. The changes of SEP and clinical symptoms correlated with each other. The spinal cord MRIs of most patients showed the lesions objectively, demonstrating primarily long-tape abnormal signals in posterior and lateral columns, iso-intense on T1 and hyper-intense on T2 without contrast. Most lesions were seen at the thoracic cord. The lesions constricted or disappeared when the clinical conditions improved. CONCLUSION: Spinal cord MRI and EP could objectively show the lesions of SCD and the change before and after the treatment. These examinations and the follow-up of disease could be helpful in the diagnosis of SCD.

Adult↗

PubMed2001

[Renal protective effects of vitamin E in diabetic rats].

OBJECTIVE: To investigate the renal protective effect of vitamin E on streptozotocin induced diabetic rats, as well as the possible mechanism involved in. METHODS: Vitamin E 20 mg x kg(-1) x d(-1) was given to diabetic rats for 8 weeks. Urinary albumin excretion (UAE) was measured by radioimmunoassay. The changes of creatinine clearance rate (Ccr), nitric oxide(NO), nitric oxide synthase (NOS) and endothelin(ET) in the plasma and renal cortical tissue, membrane protein kinase C(PKC) in the renal glomeruli were observed. RESULTS: At two weeks, diabetic rats had supernormal Ccr[(6.47 +/- 1.51) ml x min(-1) x kg(-1)], UAE[(15.60 +/- 1.64) microg/24h], NO[(37.30 +/- 3.77) micromol/L], NOS[(34.89 +/- 3.83) U/L] and PKC[(86.85 +/- 11.37) pmol x min(-1) x mg protein(-1)] of diabetic rats were increase but ET was decreased. At 8 weeks, PKC[(84.18 +/- 12.14 ) pmol x min(-1) x mg protein(-1)] and ET of diabetic rats was increased significantly, but NO[(22.75 +/- 2.89) micromol/L] and NOS[(21.34 +/- 1.92) U/L] of diabetic rats was lowered. After treated with vitamin E, Ccr[(4.46 +/- 0.49) ml x min(-1) x kg(-1)], UAE[(16.31 +/- 1.12) microg/24h], PKC[(65.19 +/- 8.83) pmol x min(-1) x mg protein(-1)] at 8 weeks were decreased significantly. Both NO[(33.13 +/- 3.77) micromol/L] and NOS[(30.16 +/- 2.89) U/L] in plasma and kidney tissue were lowered as compared with untreated diabetic group in two weeks. Vitamin E also delayed the NO and NOS lowering in 8 weeks. CONCLUSION: Vitamin E could ameliorate early diabetic renal hemodynamic abnormality via inhibiting PKC, in which inhibition of NO production might be involved. It is important to reduced the PKC activity so as to prevent or delay the development of diabetic nephropathy.

Animals↗

PubMed2001

[Relationship between circulating vascular cell adhesion molecule-1 and vascular complications in type 2 diabetes mellitus].

OBJECTIVE: To investigate the relationship between soluble vascular cell adhesion molecule-1 (sVCAM-1) and type 2 diabetes complicated with vascular diseases. METHODS: In 61 cases of type 2 diabetes with macrovascular or microvascular complications and 36 cases of healthy control, sVCAM-1 levels were measured in duplicate by enzyme-linked immunosorbent assay using the soluble VCAM-1 Kit (R& D Systems Ltd, Ablingdon, Oxfordshire, UK). RESULTS: sVCAM-1 levels in type 2 diabetes [(973.25 +/- 73.38) microg/L] were higher than these of healthy controls [(230.45 +/- 12.47) microg/L], P < 0.001. sVCAM-1 levels of type 2 diabetes with hypertension [(1263.99 +/- 87.00) microg/L] were higher than those of type 2 diabetes with microvascular complications [(726.57 +/- 95.40) microg/L], P < 0.001. CONCLUSION: There was a significant correlation between sVCAM-1 concentration and vascular complications of type 2 diabetes.

Aged↗

PubMed2001

[Evaluation on the effect of cardiovascular prevention program in Fangshan, Beijing].

OBJECTIVE: To evaluate the effect of cardiovascular prevention program in Fangshan, Beijing (BFCP) from 1991-1999. METHODS: BFCP was a comprehensive community trial covering 120,000 residents in five communities, including three as intervention communities (IC), and two as control communities (CC), in Fangshan, suburb of Beijing. The program was implemented under both population and high risk individual strategies, focusing on health education and hypertension control. The main intervention measures were as follows: (1) network setup; (2) health education and promotion; (3) health professional training; (4) detection and management of hypertensive patients. RESULTS: Baseline, midterm and final cross-sectional surveys were conducted in the year 1991, 1995 and 1999, respectively. After 9-year intervention, improvement was found in population of IC versus CC (P < 0.01 or P < 0.05) for knowledge, attitude and behavior of CVD prevention, awareness on hypertension, treatment and control rates, as well as the quantity of dietary intake of protein, potassium and calcium. Risk factors such as systolic blood pressure, diastolic blood pressure, rates of smoking and alcohol drinking declined in the population in IC versus CC (P < 0.01). The average morbidity and mortality of stroke were 18.7% (u = 2.81, P < 0.01) and 17.7% (u = 2.91, P < 0.01), significantly lower in the IC versus CC. No significant difference was identified on the morbidity and mortality of coronary heart diseases between IC and CC. CONCLUSION: The comprehensive community prevention approach was feasible and effective in the prevention and control of cardiovascular diseases in rural populations in China.

Adult↗

PubMed2001

[Application of the interaction models between the polymorphism(s) of metabolic gene(s) and environmental exposure].

OBJECTIVE: Taking GST M1 as an example to introduce analytic method of interaction models between the polymorphism(s) of metabolic gene(s) and environmental exposure in stomach cancer susceptibility. METHODS: Using community-based case-control design, combined with molecular biological techniques (PCR) and multiple variables logistic regression models, we analyzed 112 intestinal types of stomach cancer cases with endoscopy and pathology diagnosis in the Yangzhong City Hospital during January 1997 and December 1998. A total of 675 controls were selected from persons who had no history of digestive system cancers. RESULTS: After adjustment of confounding variables with both GST M1 null genotype and history of ever tobacco smoking, the results showed a significant types of 4 gene-environment interaction. Interaction index (gamma) value was 3.38 and OR(eg) value was 8.40, suggesting that a super multiplicative interaction occurred. The results also showed that GST M1 null genotype had a high exposure-gene effect interaction with tobacco smoking (pack year), while gamma values were 0.995, 2.085 and 2.157 respectively. A low exposure-gene effect interaction was found in GST M1 null genotype with the amount of (kg x year) alcohol consumption while gamma values were 1.01 and 0.97 respectively. CONCLUSION: Logistic regression model can be used to evaluate gene-environment interaction and dose-response of exposure-gene effect.

Alcohol Drinking↗

PubMed2001

[Studies of the clinicopathological changes of eight patients with lipid storage myopathy].

OBJECTIVE: The clinicopathological analysis of eight patients with lipid storage myopathy are presented. The pathogeny and therapeutic effect are probed into. METHODS: Eight cases of lipid storage myopathy diagnosed by muscle biopsies with microscopic and electron-microscopic examination are analyzed. Quadriceps or biceps were biopsied. Muscle samples were stained with routine histology and histochemical enzyme and inspected by microscopy. Thin sections were stained with uranyl acetate followed by lead citrate prior to examination in a electron microscopy. Also, the therapeutic drugs of eight patients were evaluated. RESULTS: Vacuole or crack of muscular fibers involved all eight patients. Sudan Black B and Oil Red O stains demonstrated increase of lipid droplets within muscle fibers. Ultrastructural examination revealed numerous lipid droplets dispersed throughout the residual myofilaments. Three cases with pathologic changed muscular fibers occupying less than 1/5 were belong to low-grade, two cases (between 1/5 to 1/3) were moderate, three cases (more than 1/2) were severe. There was one case accompanying glycogen storage disease. One case was concomitant with deficiency of cytochrome C oxidase. After prednisone treatment, seven cases had greatly improved and one case failed to respond to. Treatment using vitamin B(2) together with other vitamins brought about a striking effect. Carnitine was very effective on the patients with system deficiency of carnitine. CONCLUSIONS: The pathogeny of lipid storage myopathy is varied. The confirmed diagnosis is depend on pathological features of muscle biopsy. Treatment with prednisone, carnitine, vitamins and food containing carnitine rich is very effective. It should be select the special treatment method if the pathogeny is clear.

Adolescent↗

PubMed2001

[Pathogen detection in the myocardial lesion using in situ RT-PCR in mice induced by experimental CoxB(3m) virus infection].

OBJECTIVE: To detect the location of pathogens in myocardium using in situ RT-PCR technique in order to study the pathogenetic course of the myocardial lesion induced by CoxB(3m) virus infection in mice. METHODS: (1) Thirty and fifty Balb/c mice were used respectively to establish the acute and chronic CoxB(3m) infected models, with another 25 healthy mice as the controls; (2) KS400 image analysis system (Germany) was used to measure the cardiac chamber area and the left ventricular wall thickness of the chronic infected mice and the controls; (3) CoxB(3m) virus in myocardial tissue was detected using in situ RT-PCR by direct incorporated technique which employed nucleotide labeling by anti-digoxin antibody and bonded with alkaline phosphatase (anti-dig-AKP method). RESULTS: Picture analysis indicated that the left ventricular chamber area was enlarged and the left ventricular wall was thinner in the chronic repeated virus infected models than those of the controls. With in situ RT-PCR, positive signals for Coxsackie virus B(3m) RNA were detected not only in the myocardium of the acute Balb/c mice models but also in the myocardium of the chronic mice models. CONCLUSION: Coxsackie virus B(3m) is able to induce pathologic lesions by exhibiting positive CVB-RNA signals in both acute and chronic models in mice. In the chronic experimental models, the cardiac chamber is enlarged while the ventricular wall is thinned which demonstrates the association with persistent infection of Coxsackie virus B(3m) virus.

Animals↗

PubMed2001

[Primary cutaneous CD30-positive anaplastic large cell lymphoma].

OBJECTIVE: To analyse the clinical manifestations, pathological and immunohistochemical features of 9 cases of primary cutaneous CD30-positive anaplastic large cell lymphoma (ALCL) in order to improve the criteria for its early and differential diagnosis. METHODS: Histopathological method was used to study the morphological characteristics. Immunohistochemical stainings (SP or ABC method) for leukocyte common antigen (LCA), CD20, CD30, CD45RO, CD68, epithelium membrane antigen (EMA), cytokeratin (CK) and HMB45 were applied to the routine paraffin sections of all the 9 cases. RESULTS: The 9 cases of primary cutaneous CD30-positive ALCL consisting of 6 males and 3 females, aged 31 to 84 years (mean 58.2 years). All patients presented with subcutaneous masses or papular eruptions on lower trunk and extremities. Histopathologically, the lesions were composed of numerous large round, oval or pleomorphic cells with abundant cytoplasm, amphophilic or basophilic, and large nucleus with distinct nucleolus. Mitosis was often observed. Multinucleated giant cells and Reed-Sternberg cells may be seen. The neoplastic cells displayed positive antigen markers for CD30 in 9 cases, 6 positive for CD45RO and 3 cases negative for both CD45RO and CD20. Two patients died of metastasis, 2 patients experienced recurrence and 5 patients still well during follow-up. CONCLUSIONS: Primary cutaneous CD30-positive ALCL is a morphologically distinctive neoplasm with a comparatively favorable prognosis. This tumor can be differentiated from other malignant tumors on the basis of histopathologic features and positive CD30 which is of significance.

Adult↗

PubMed2001

[Retrospective clinical analysis of the emergent comprehensive repair of electrical injury].

OBJECTIVE: To summarize the experience with clinical emergent comprehensive repair of electrical injury. METHODS: One hundred fifty five cases of electrical injury during past 14 years were analyzed in terms of the emergent repair methods on the basis of medical records and photographs. RESULTS: In this group of patients the average interval between injury and hospitalization was 14.9 +/- 21.5 hours, And 108 cases (70%) were admitted to hospital within 2 hours post injury. The average time between admission and first operation was 3.74 +/- 9.89 hours, and 70% of them was within 1 hour after admission. Four hundred and fifty nine wounds were debrided and 296 strips of tendons, 19 strips of blood vessels, 196 pieces of bone or joints and 16 strips of nerves, which were exposed, devitalized or necrotic, were saved. The grafting repair of tendons, bones, nerves and blood vessels was done in 24 case times. Three hundred and ninty eight tissue flaps were employed in 459 wounds. CLS (NS + Lidocaine + amphicol) solution was employed to irrigate underneath the flaps for 24 similar 96 hours or longer. Composite dan -- shen root (scatellaira indica) solution was given to all the patients by injection. As a result, the excellent rate of function and contour was 84.8%. The unsatisfactory and amputation rate was 9.6%. The rate of extremity and/or finer amputation was 1.8%. The rate of total limb loss including those lost instantly after injury was 9%. CONCLUSION: Emergent comprehensive management of electrical injury was a feasible and effective method for decreasing extremity loss.

Adolescent↗

PubMed2001

[Analysis of bacterial resistance to antibiotics in a burn ward].

OBJECTIVE: To analyze the bacterial resistance to antibiotics in a burn ward in the past 4 years. METHODS: Six hundred and fifty strains of bacteria were isolated from the wounds and blood of burn patients admitted to our hospital in recent 4 years. Slip agar diffusion method was employed to carry out antibiotic sensitivity test, and the results were interpreted according to the NCCLS criteria. Extended spectrum beta -- lactamases (ESBLs) were detected by double slips synergic test in 45 strains of Klebsiella Pneumoniae. RESULTS: Only vancomycin was effective for staphylococcus aureus, and Imipenem was effective for E. coli, E. cloacae and K. pneumoniae. But P. aeruginosa was only susceptible to polymyxin -- B. In addition, Thirty -- eight out of 45 strains of K. pneumoniae were ESBLs positive with the incidence of 84.4%. CONCLUSION: The high resistance of the bacteria to most antibiotics in our burn ward might be related to the high production of beta -- lactamases and especially ESBLs. Imipenem remained stable to ESBLs and remained highly effective for most bacteria. But antibiotics with beta -- lactamases inhibitor became less effective in recent years. The resistance rate of P. aeruginosa to Imipenem increased gradually in recent years. This might be due to the abuse of Tienam in burn patients, which stimulated the bacteria to produce a new metallic beta -- lactamases transfected by plasmid, decreasing the production of bacterial outer membrane proteins and inducing a change in membranous permeability.

Anti-Bacterial Agents↗

PubMed2001

[A study of methicillin -- resistant staphylococcus aureus (MRSA) in a burn unit with repetitive -- DNA -- sequence -- based PCR fingerprinting].

OBJECTIVE: To investigate the distribution and spread of MRSA in a burn ward, so as to explore the measures of the prevention, surveillance and control of hospital infection in a burn ward. METHODS: Five hundred and four specimens were isolated from the wounds and nasal vestibules of burn patients, the hands and nasal vestibules of medical staffs and lay attendants and the surfaces of various equipments. From these specimens, 58 strains of MRSA and 43 methicillin -- sensitive staphylococcus aureus (MSSA) were isolated. The genome DNA of isolated MRSA strains was analyzed by repetitive DNA -- sequence -- based PCR analysis. RESULTS: MRSA strains were isolated from the burn wounds in 22 of 41 (53.7%) patients, and 5 from the nasal vestibules. Moreover, among 19 medical staffs, MRSA strains were isolated from the hands of 9 persons, but not from the nasal vestibules. From the hands in 9 of 43 lay attendants and the nasal vestibules in 2 MRSA strains were found. Thirteen MRSA strains were isolated from 193 specimens from the surrounding items. It was indicated by repetitive DNA -- sequence -- based PCR analysis of the genome DNA of isolated MRSA strains that there existed homologous strains around the patients' wounds, in the burn wounds, on the skin of medical staffs and lay attendants and also surrounding equipments. CONCLUSION: (1) There was wide spread presence of MRSA homologous strains in the burn ward. (2) It was indicated by repetitive DNA -- sequence -- based PCR analysis of the genome DNA of isolated MRSA strains that there was cross infection among burn patients. The source of the infection of MRSA in burn ward was burn patients, and the route of the infection was hands of medical staffs and lay attendants. (3) MRSA is wide -- spread. The contamination of the hands and the environment was potential risk factor of MRSA outbreak in the burn unit.

Burn Units↗

PubMed2001

[An Experimental Study on the diagnosis of high voltage electrical injury by means of biological oxygen consumption measurement].

OBJECTIVE: To investigate the degrees of the degeneration or the necrosis of muscle tissue injured by high voltage electricity as determined by biological oxygen consumption measurement. METHODS: An animal model with high voltage electricity designed in our laboratory was employed in the study. Muscle tissue sized 12 mm x 10 mm x 1 mm at electric burn produced 5 spots of the rabbit injured legs was harvested for the measurement of tissue oxygen consumption for 100s at 2, 8, 14, 24, 48 postburn hours and 5 postburn day. At the same time histological examination was made. RESULTS: One hundred and eighty two pieces of tissue samples were examined. Among them 20 samples with oxygen consumption below 8.7 kpa/100s (43.5%) showed necrosis under LM, while 157 samples with oxygen consumption of 16.9 to 8.78 kpa/100s (84% similar 44%) showed histological necrosis and different degrees of mixed degeneration and normal tissue, and 5 samples with oxygen consumption above 17 kpa/100s (85%) showed normal histological structure. CONCLUSION: The lower the tissue oxygen consumption was, the more severe the tissue degeneration and necrosis were. Oxygen consumption measurement could be valuable in the determination of the degree of tissue injury inflicted by electricity in terms of its convenience, rapidity and accuracy.

Animals↗

PubMed2001

[Changes of L-selectin expression on polymorphonuclear leukocytes and its role during polymorphonuclear leukocytes sequestration in lungs of rats with acute lung injury].

OBJECTIVE: To explore the changes of L-selectin protein expression on peripheral blood polymorphonuclear leukocytes (PMN) and their role during PMN sequestration in the lungs of rats with acute lung injury (ALI). METHODS: The model of ALI in rat was established by intravenous injection of E. Coli endotoxin (ET). The protein expression of L-selectin on peripheral blood PMNs was measured by immunofluorescence and flow cytometry. Myeloperoxidase (MPO) enzyme analysis and histology were employed to estimate the quantity of PMN sequestration in lungs of rats during ALI. RESULTS: (1) The protein expression of L-selectin on PMN after injection of ET (7.8 +/- 1.6) was all significantly lower than that in control group (10.5 +/- 2.1, P < 0.05). (2) MPO activity increased significantly after injection of ET in comparison with the control group [0.069 +/- 0.011) U/mg lung tissue, P < 0.01]. The difference was not significant between the fucodin treated 5 min group [(0.391 +/- 0.071) U/mg lung tissue] and ET-treated 5 min group [(0.359 +/- 0.074) U/mg lung tissue]. But MPO activity was significantly lower in fucodin-treated 15 min group [(0.396 +/- 0.061) U/mg lung tissue] than that in ET-treated group at same time point [(0.490 +/- 0.069) U/mg lung tissue, P < 0.05]. CONCLUSIONS: (1) In physiological state, L-selectin are consistently expressed on the surface of PMN membrane. The protein expression of L-selectin on PMNs reduces rapidly after injection of ET and is the lowest at 15 min, then gradually increases. (2) The early stage of PMN sequestration in lungs is not dependent on L-selectin, but L-selectin is still important for maintaining a continual PMN sequestration in lungs of rats with ALI.

Animals↗

PubMed2001

[Comparative study on antibody levels of vaccination group and control group after 4 years of immunized with type B inactivated vaccine against hemorrhagic fever with renal syndrome].

OBJECTIVE: To compare the antibody level of vaccine group and control group in a randomized, controlled trial of type B inactivated vaccine against hemorrhagic fever with renal syndrome(HFRS) in Jiande County of Zhejiang Province and to find out if there is enhancement of subclinical infection about type B inactivated vaccine against HFRS. METHODS: The number of sera collected (July 1994-July 1998) in vaccine group immunized type B inactivated vaccine against HFRS and control group were 305 and 283 persons in Jiande County respectively, and indirect immunofluorescent antibody method(IFAT) was used for testing the titer of HFRS IgG in sera. The cut-off values were determined on IFA titer of the second sera in control group with positive and negative IFA before vaccine was given respectively, and the subclinical infection of positive and negative IFA of the first sera in vaccine group was evaluated. RESULTS: When taking different cut-off values of subclinical infection, there were no statistical differences in HFRS IFA positive rates between vaccine group and control group of positive IFA in the first sera. In vaccine group and control group of negative IFA before vaccine given, also the IFA positive rates were not statistically different in these groups. There were no statistical differences of IFA GMT and IFA sero-negative rate between vaccine group and control group. CONCLUSIONS: The investigation showed there was no statistical difference in antibody level between vaccine group and control group, so there could not be enhancement of subclinical infection of vaccine group receiving type B inactivated vaccine against HFRS.

Adolescent↗

PubMed2001

[HIV genomic mutation on antiretroviral therapy].

BACKGROUND: To study the characteristics of HIV genomic mutation on antiretroviral therapy. METHODS: HIV DNA and HIV RNA were detected by using Taq-PCR, RT-PCR, LD-PCR, hybridization, and oligonucleotide 32P- probes against gag, tat, nef, LTRU3, etc. RESULYS: The HIV RNA load in serum declined significantly, CD4 lymphocyte count increased, copies of gag region of HIV DNA decreased, and full-length HIV DNA disappeared. Bands showing gag segments hybridized with 32P-probe appeared negative or less clear in some cases after antiretroviral therapy. However, the regions hybridized with probes Xho-nef and LTR U5 were apparently enhanced and some drawbands were seen. CONCLUSIONS: HIV gene may experiece obvious mutation on antiretroviral therapy.

Adult↗