Biomedical subjects

X Wu

Publications and source records attributed to X Wu.

At least 217 records · Page 12Linked to original sources

PubMed2001

[Percutaneous transluminal septal myocardial ablation in patients with hypertrophic obstructive cardiomyopathy: immediately and middle-term follow-up results].

OBJECTIVE: To evaluate the immediate and follow-up results of percutaneous transluminal septal myocardial ablation (PTSMA) in patients with hypertrophic obstructive cardiomyopathy (HOCM). METHODS: 15 patients with HOCM refractory to medication successfully underwent PTSMA under myocardial contrast echocardiography. RESULTS: The left ventricular outflow gradients (LVOTG) were reduced significantly (77.93 +/- 22.00) mm Hg to (14.8 +/- 15.0) mm Hg (P < 0.0001) immediately with a mean decrease of (5.75 +/- 2.87) mm Hg (P < 0.001) in left ventricular end diastolic pressure. The scores of SAM and mitral regurgition During a mean of 8.6 +/- 3.8(6-22) months follow-up, the patients heart function (NYHA) were greatly improved(3.4 +/- 0.5 vs 1.1 +/- 0.4, P < 0.001), and disappearance of angina pectoris, and exercise endurance improvement with steady hemodynamic improvement. CONCLUSION: PTSMA is effectively non-surgical treatment method for drug-refractory patients with HOCM.

Adolescent↗

PubMed2001

Chimera formation of platelet GP II b Bak a/b by intrauterine transplantation of fetal liver stem cells.

OBJECTIVE: To investigate whether artificial heterozygous chimeras of platelets can be established by intrauterine transplantation of fetal liver stem cells and evaluate its potential use for the treatment of Glanzmann thrombasthenia. METHODS: Platelet glycoprotein (GP) II b Bak a/b (or GP II b I le843Ser) was used as a genetic marker. A homozygous 16-week-old Bak a/a fetus (as donor) and a homozygous 16.5-week-old Bak b/b fetus (as recipient) were screened from 42 pregnant women hospitalized for abortion. PCR with allele specific primers and FOK I digestion based on PCR products were used. Aborted donor fetal liver cell suspensions were prepared and intrauterine transplantation was carried out by infusion of 4 ml fetal liver cells (22 x 10(5)) into the recipient umbilical vein under ultrasonic visualization. RESULTS: At gestation termination (abortion), 21 days after transplantation, chimera GP II b Bak a/b of the recipient were detected by FOK 1 digestion based on PCR from DNA and RT-PCR from platelet RNA. CONCLUSION: Intrauterine transplantation of fetal liver cell may provide an effective way for curing GT or other inherited diseases.

Adult↗

PubMed2001

[Experience and evaluation of extended resection and lymph nodes dissection in stage T3-4N2M0 lung cancer].

OBJECTIVE: To report our experience of surgical treatment for stage T3-4N2M0 lung cancer. METHODS: One hundred thirty one patients with lung cancer in stage T3-4N2M0 were surgically treated by extended tumor resection, lymph nodes dissection and resection of ipsilateral mediastinal pleura and fatty tissue. RESULTS: The 3-year survival rate of patients in stage T3N2M0 with squamous-cell carcinoma and adenocarcinoma was 34% and 18%, respectively. That for stage T4N2M0 patients was only 13% and 5%, respectively. The survival rate was not improved in patients who received combination treatment in addition to surgery. CONCLUSION: The indication of extended resection with lymph node dissection in the treatment of T3-4N2M0 lung cancer is limited.

Adenocarcinoma↗

PubMed2001

[A contour map segmentation for laser scanning confocal microscopic biomedical images].

We propose a contour map segmentation method for laser scanning confocal microscopic (LSCM) biomedical images. In this method, an image is first segmented into sub-regions by contour map. Then the sub-regions are merged from top to bottom or from bottom to top, according to a segmentation criterion of intensity homogeneity. The merging procedures continue until no region needs to be merged. The advantage of this method is that the objects with wide intensity ranges and different intensity homogeneity can be segmented properly. In this paper, the region segmentation by contour map is first addressed. Next a region merging according to a segmentation criterion of intensity homogeneity is presented, and then the design of a linear intensity homogeneity segmentation criterion is introduced. Two examples of image segmentation by this method are given at the end of this paper.

Algorithms↗

PubMed2001

[Renal protective effects of vitamin E in diabetic rats].

OBJECTIVE: To investigate the renal protective effect of vitamin E on streptozotocin induced diabetic rats, as well as the possible mechanism involved in. METHODS: Vitamin E 20 mg x kg(-1) x d(-1) was given to diabetic rats for 8 weeks. Urinary albumin excretion (UAE) was measured by radioimmunoassay. The changes of creatinine clearance rate (Ccr), nitric oxide(NO), nitric oxide synthase (NOS) and endothelin(ET) in the plasma and renal cortical tissue, membrane protein kinase C(PKC) in the renal glomeruli were observed. RESULTS: At two weeks, diabetic rats had supernormal Ccr[(6.47 +/- 1.51) ml x min(-1) x kg(-1)], UAE[(15.60 +/- 1.64) microg/24h], NO[(37.30 +/- 3.77) micromol/L], NOS[(34.89 +/- 3.83) U/L] and PKC[(86.85 +/- 11.37) pmol x min(-1) x mg protein(-1)] of diabetic rats were increase but ET was decreased. At 8 weeks, PKC[(84.18 +/- 12.14 ) pmol x min(-1) x mg protein(-1)] and ET of diabetic rats was increased significantly, but NO[(22.75 +/- 2.89) micromol/L] and NOS[(21.34 +/- 1.92) U/L] of diabetic rats was lowered. After treated with vitamin E, Ccr[(4.46 +/- 0.49) ml x min(-1) x kg(-1)], UAE[(16.31 +/- 1.12) microg/24h], PKC[(65.19 +/- 8.83) pmol x min(-1) x mg protein(-1)] at 8 weeks were decreased significantly. Both NO[(33.13 +/- 3.77) micromol/L] and NOS[(30.16 +/- 2.89) U/L] in plasma and kidney tissue were lowered as compared with untreated diabetic group in two weeks. Vitamin E also delayed the NO and NOS lowering in 8 weeks. CONCLUSION: Vitamin E could ameliorate early diabetic renal hemodynamic abnormality via inhibiting PKC, in which inhibition of NO production might be involved. It is important to reduced the PKC activity so as to prevent or delay the development of diabetic nephropathy.

Animals↗

PubMed2001

[A 10-year longitudinal study of the progression of destructive periodontal disease in adult and elderly].

OBJECTIVE: To study the progression of destructive periodontal disease in adult and elderly aged 20 to 80. The examinees were followed 10 years to determine whether the rates for progression of periodontal disease were different than the populations in the more developed countries. METHODS: In 1984, at baseline, 587 subjects had been examined and by 1994, 440 of them still available for follow-up study, and 398 persons remained dentate. Tooth mobility, plaque, calculus, gingival conditions, attachment levels, and probing depths on 4 sites of each tooth present were checked and recorded by the same two examiners. RESULTS: All subjects had > or = 2 mm attachment loss over 10-year period. Some teeth, such as mandibular incisors and maxillary molars, had higher progression rate than others. The mean attachment loss rate did not differ significantly between age groups, and were similar to those reported for populations in the more developed countries. CONCLUSIONS: The rate for progression of periodontal disease in this study were not significantly different between the examinees and the populations in more developed countries; and also were not significantly different among the different age groups. The causes of destructive periodontal disease in humans are more likely intrinsic factors.

Adult↗

PubMed2001

[Effect of fly ash-filtered mud mixture on soil properties and radish yield and quality].

Based on pot culture experiment, the effect of fly ash-filtered mud mixture on soil biochemical properties, radish yield and its quality, and heavy metal accumulation in both soil and radish was examined. The mixture was made by mixing fly ash and filtered mud in proportion 1:1(w/w) and adding small amount of inorganic fertilizer. Its Cd, Pb, Cr, As and Hg contents were much lower than those requested by state control criteria. After applying this mixture in a definite amount, no significant accumulation of heavy metals was found in both soil and radish, the pollution index of heavy metals was < 1, the quantities of soil bacteria increased notably, and the activities of soil urease, phosphates and cellulosase also raised significantly. The application of the mixture promoted radish growth and its development and metabolism. The reducing sugar and vitamin C in radish also increased markedly. The results indicated that the use of adequate amounts of the mixture did not cause any obvious heavy metal pollution in both soil and radish, but could improve soil fertility, and raise radish yield and its quality remarkably.

Agriculture↗

PubMed2001

Different expression of estrogen receptors alpha and beta in human myometrium and leiomyoma during the proliferative phase of the menstrual cycle and after GnRHa treatment.

Uterine leiomyomas (uterine fibroids) are sex-steroid dependent benign tumors. Limited knowledge is available regarding the role of estrogen and their receptors in the regulation of fibroids in premenopausal women, and in their shrinkage after treatment with a gonadotropin-releasing hormone analogue (GnRHa). The expression of the two subtypes of the estrogen receptor (ER), ER alpha and ER beta, was studied in leiomyoma and homologous myometrium from women in the proliferative phase of the menstrual cycle and from women treated with GnRHa. The mRNA levels of ER alpha and ER beta were monitored by solution hybridization and in situ hybridization, and receptor proteins were detected and localized by immunohistochemistry. Both ER alpha and ER beta were present in the leiomyomas and homologous myometrium. The ER alpha mRNA level in the leiomyomas was higher than in the surrounding myometrium. The ER beta mRNA level was lower than that of ER alpha in both groups. ER beta immunoreactivity was lower in leiomyomas when compared with the myometrium after GnRHa treatment, while ER alpha was higher in the leiomyomas. The present results imply that the increased ratio of ER alpha/ER beta observed in the fibroids after GnRHa treatment could reflect or be the cause of the shrinkage of the leiomyoma, which is the clinical outcome of this treatment.

Adult↗

PubMed2001

Retrospective study of mechanical ventilation in treating multiple trauma accompanied with craniocerebral injury.

OBJECTIVE: To explore the importance of mechanic al ventilation (MV) in treating multiple trauma mainly accompanied with cerebral injury and improve the treatment level. METHODS: All 116 patients who suffered from multiple trauma accompanied with mainly cerebral injury were analyzed. Rank correlation and Chi-square test were made between MV with GCS, ISS, and whether shock or not. RESULTS: The MV was correlated with GCS, ISS scores, and whether shock or not markedly. Furthermore, when GCS or=35, the ratio of MV was about 85%. CONCLUSIONS: During the treatment of multiple trauma accompanied with cerebral injury, the principle of "early MV, early withdraw" and "individualism" should be insisted on. For patients accompanied with shock, the optimum PEEP is very important. Proper and active MV is of great importance and can improve the treatment level markedly.

Adolescent↗

PubMed2001

[Keratoprosthesis design and biological response to a synthetic cornea].

OBJECTIVE: To evaluate the biological response to three different designs of synthetic cornea in vivo. METHODS: All devices consist of a transparent center and a porous blown periphery. Thirty-five devices were implanted into 35 rabbits' corneas and followed up to more than 6 months. Rabbit corneal stromal cells were inoculated onto the anterior surface of the synthetic cornea and cultured in vitro for one week before the operation. The anterior surface of the optic center hydrogel was modified with radio frequency (rf)-argon plasma treatment. RESULTS: Fibroplasia occurred 2 weeks after surgery and collagen was detected by 28 days. The anterior chamber was normal without detectable leakage of aqueous humor. Basic fibroblast growth factor (bFGF) was detected at the interface between the device and the tissue by 42 days. CONCLUSIONS: It is demonstrated that rabbit limbal epithelial cells can migrate onto the synthetic cornea in vivo.

Animals↗

PubMed2001

[Evaluation of protective efficacy of two tuberculosis DNA vaccines by lung histopathological analysis].

OBJECTIVE: To evaluate the protective efficacy of MPT64 and ESAT6 DNA vaccines from M. tuberculosis. METHODS: BALB/c mice were randomly divided into five groups and subjected to the following treatments respectively, i.e. immunized with saline (A), plasmid vector (B), M. bovis BCG (C), MPT64 DNA vaccine (D) or ESAT6 DNA vaccine (E); and then infected by intraperitoneal injection with M. tuberculosis H37Rv. The lung histopathological changes were observed 5 or 10 weeks after infection by microscopy. RESULTS: At 5 weeks after infection, the lung lesions in the mice of group A and B had inflammatory infiltration with epithelial cell granulomas. In the mice of group C, main pathological changes were epithelial cell granulomas with moderate granulate hyperplasia in alveolar walls. The lung lesions of 3 mice in group D and 1 mice in group E were similar to those seen in the mice in group A and B. The lung lesions of 2 mice in group D and 4 mice in group E were similar to those seen in the mice in group C. At 10 weeks after infection, their tuberculous pneumonia tended to recovery. For the mice in groups A, B and D, their lung pathology exhibited tuberculous granulomas consisted of numerous macrophages, lymphocytes and a few epithelial cells with moderate granulate hyperplasia in alveolar walls. For the mice in groups of C and E, their lung developed epithelial cell and lymphocytic granulomas with moderate to severe granulate hyperplasia in alveolar walls. Lung tissue necrosis was not observed in any mouse. CONCLUSIONS: MPT64 and ESAT6 DNA vaccines from M. tuberculosis could enhance immunity against M. tuberculosis. The protective efficacy of ESAT6 DNA vaccine is stronger than that of MPT64 DNA vaccine, but not stronger than that of Mycobacterium bovis BCG.

Animals↗

PubMed2001

[Evaluation of p53, p21waf1, and PCNA in the diagnosis on lung cancer].

OBJECTIVE: To evaluate the diagnostic value of p53, p21waf1 and PCNA on diagnosis of lung cancer. METHODS: One hundred and fourteen lung cancer patients and 89 cases with benign pulmonary diseases were studied on the expression of p53, p21waf1 and PCNA by immunohistochemical staining. The differences of p53, p21waf1 and PCNA expression status between these two groups were compared and the diagnostic value of these three markers was evaluated. RESULTS: (1) The positive rates of p53, p21waf1 and PCNA expression in lung cancer were 47.37%, 75.44% and 80.70%, all significantly higher than that in benign pulmonary diseases (P < 0.001), with odds ratio 39.15, 5.75, 6.76 respectively. (2) The positive likelihood ratio of p53 was 21.08, higher than that of p21waf1 and PCNA. Specificity and Youden index were also higher than those of p21waf1 and PCNA, but the sensitivity of p53 was low. (3) The sensitivity and specificity of p53 and PCNA parallel test were 89.47% and 60.67% respectively. CONCLUSIONS: (1) The expression of p53, p21waf1 and PCNA in lung cancer cases were all upregulated. These three markers all seemed to have strong links with lung cancer. (2) p53 was shown a good marker for lung cancer diagnosis. (3) The diagnostic value of p53 and PCNA parallel test was rather high.

Biomarkers, Tumor↗

PubMed2001

[Study on the reversion of drug resistance in human cervical cancer cell lines].

OBJECTIVE: To determine the resistance reversion of mitomycin (MMC) by 3'-Keto-bmt1-val2-cyclosporin (SDZ PSC 833) in human cervical cancer in vitro and in vivo. METHEDS: A xenografted mitomycin resistant mice model of cervical cancer was devolped. The reversion of mitomycin resistance by SDZ PSC 833 (1 or 3 mg/L) was detected from human cervical cancer cell (Hela) and its resistant subline Hela/MMC in vitro and in vivo. Studies in vitro include drug resistance reversion experiment and the changes of morphology. Studies in vivo including tumor volume and tumor related histopathological changes in the autopsied specimen were evaluated by comparing random sections of each group. RESULTS: Nontoxic doses of SDZ PSC 833 could result in almost partial reversion of MMC-resistance of Hela/MMC. In vivo studies also showed SDZ PSC 833 augmented the growth inhibitory effect of mitomycin on Hela/MMC xenografted in nude mice. CONCLUSION: SDZ PSC 833 can overcome mitomycin resistance of Hela/MMC in vitro and in vivo, so SDZ PSC 833 will be a better candidate clinically for reversing multidrug resistance.

Animals↗

PubMed2001

[Comparing the two neurobehavioral toxicological methods on the toxic effect of methyl mercury].

To compare the two experimental methods for testing neurobehavioral toxicological effects, methyl mercury chloride at the dosage of 0.00, 0.01, 0.05 and 2.00 mg/(kg.d) was force-fed to pregnant Wistar rats during the 6-9th day of conception. The offspring of the 4 groups were randomly selected for Y maze or operant behavior test at the age of 7 or 10 weeks, respectively. The reinforcements of the performance were electric shock for Y maze and food pellets for operant behavioral test. The results showed that no sex-dependent effects were observed both in maze and operant behavior tests. The performances of offspring in 3 exposure groups were worse than that of control group both in Y maze and operant behavior tests(P < 0.05). It was demonstrated that a clear dose-effect relationship in maze test on error time(rs = 0.257, P < 0.05), arrival time(rs = 0.216, P < 0.01); in operant behavior test on differential reinforcement of high rate (rs = -0.7273, P < 0.01), but not on differential reinforcement of low rate(rs = -0.2238, P > 0.05). It is concluded that prenatal methyl mercury exposure caused some negative effects on the development of nervous system. But the negative reinforcement by human operation in Y maze test could affect the results. The positive reinforcement and automatic monitoring in operant behavioral test gave more reliable results.

Animals↗

PubMed2001

Relation between alkaline phosphatase in gingigval crevicular fluid of implant teeth and the curing result.

OBJECTIVE: To discover the relation between alkaline phosphatase (ALP) in gingival crevicular fluid (GCF) of implant teeth and the curing results. METHODS: We measured the ALP level in GCF among 56 cases of implant teeth which included 2 failed cases, 5 cases with bad oral hygiene and gingivitis, and compared it with that in the normal group composed of 10 persons. RESULTS: The ALP levels in normal group and success implant group showed no difference. The ALP levels in normal group and success with gingivitis group showed obvious difference. The ALP levels of the 2 failed cases are the highest of all. CONCLUSIONS: The ALP level in GCF is an important index in evaluating the curing result of the implant teeth.

Adult↗

PubMed2001

[Raman spectra of Cu/Zn superoxide dismutase and L-cysteine used as an electron transfer promoter].

In author's laboratory, it has been demonstrated with electrochemical techniques that L-cysteine can be used as an effective electron transfer promoter to accelerate the electron transfer process of Cu/Zn superoxide dismutase on gold electrode. In order to reveal the mechanism of this promotion, confocal microscopic Raman spectroscopic technique is employed here to obtain Raman spectra of solid state SOD and L-cysteine modified gold electrode. Moreover, Raman spectra of L-cysteine modified gold electrode with two different approaches are also obtained. Analysis and discussion for these Raman spectra are reported.

Cysteine↗

PubMed2001

[Determination of Avermectin B1 content by first-order derivative spectrum analysis].

After acquiring the ultraviolet absorption spectrum and its first order derivative spectrum distributions of Avermectin B1 by using UV-2401 PC spectrophotometer and spectrum-measuring system, we found that coordinates of the peak A252.2 and the valley A256.8 are not disturbed by the different forms of preparations. We considered that delta A = A252.2-A256.8 is the reliable ground for quantitative analysis of Avermectin B1 content in order to reduce error. The standard regression equation c = 272.7 delta A-0.161 and the related coefficient r = 0.9996 are obtained through measurements of the standard preparations. Then the Avermectin B1 content and the average recovery rate of different preparations are determined and the result indicates that this method is especially suitable for determining the Avermectin B1 content.

Anthelmintics↗

PubMed2001

[FTIR studies of compounds of 2-D networks].

Recently, there is increasing interest in the study of 2-D square network materials which have inner cavities as zeolites, expected to find applications in catalysis, sensor and absorbent. Here, the synthesis of two compounds of [[Cd(4,4'-bpy)2.2H2O](NO3)2.4H2O] and [[Cd(4,4'-bpy)2NO3H2O].NO3.(C13H11NO)2] have been reported. The FTIR spectra of those clusters and the structure-sensitive bands are studied.

English Abstract↗