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Biomedical subjects

X Weng

Publications and source records attributed to X Weng.

At least 37 records · Page 2Linked to original sources
PubMed1999

The theory of "expelling pathogens regardless of hard stool", a significant contribution of Wu Youke.

Though there are famous theories of "assorted Qi", "membranous origin", the contribution of Wu Youke's Wen yi lun (On Warm Pestilence) of the theory of " expelling pathogens regardless of hard stool" (EPRHS) is even more important. The main points of this theory includes: it is essential to expel pathogens at an early stage, while for attacking therapy, expelling pathogens with rhubarb is essential. EPRHS elucidates the significance of expelling pathogens in attacking therapy theoretically, and elevates the efficacy clinically with profound influence in later ages. In the past 2 decades, the early application of rhubarb to elevate the therapeutic effect for the treatment of pneumonia, epidemic meningitis, and Bencephalitis even in the absence of hard constipated stool marks the significance of Wu's such theory.

Catharsis↗
PubMed1999

[Linked marker analysis of bronchial asthma in China].

OBJECTIVE: To study the clinical application value of genetic linkage analysis for bronchial asthma with two STRs-D5S436 and D5S658. METHODS: Polymorphism of the two STRs were amplified by polymerase chain reaction, and the alleles identification were performed with denaturing polyacrylamide gel electrophoresis and silver-staining techniques. RESULTS: In 92 unrelated Chinese individuals, 8 alleles and 22 phenotypes in D5S436, 9 alleles and 28 phenotypes in D5S658 were observed. The heterozygosities were 78% and 73% respectively. The polymorphism information contents (PIC) were 0.76 and 0.80 respectively. Linkage analysis showed linkage of asthma related phenotypes (including asthma) with D5S436 on chromosome 5q (lod = 2.490, theta = 0.1). CONCLUSIONS: D5S436 and D5S658 were good genetic markers. Linkage analysis demonstrates that locus on chromosome 5q31-33 maybe was linked to the development of asthma in Chinese population.

Adolescent↗
PubMed1999

[A study of airway reactivity and nature of chronic asthmatic bronchitis].

OBJECTIVE: To observe the airway reactivity with methacholine provocation and exercise challenge of chronic asthmatic bronchitis and to assess preliminarily the nature of chronic asthmatic bronchitis. METHODS: 42 patients with bronchial asthma, 29 with simple chronic bronchitis and 27 with chronic asthmatic bronchitis were selected. The study protocol consisted of inhalation challenge with methacholine and exercise challenge. Count of blood eosinophil cells and level of blood total IgE were studied. Serum eosinophil cationic protein (ECP) concentration was measured in the asthmatic bronchitis group. RESULTS: The positive ratio to methacholine response was 77.8% in chronic asthmatic bronchitis group, whereas the ratios were 100.0% and 41.4% in bronchial asthma and simple chronic bronchitis groups respectively. According to the response to exercise challenge, patients with chronic asthmatic bronchitis were divided into positive and negative groups. The cumulative dose of methacholine producing a 20% fall in FEV(1) (PD(20)-FEV(1)) was different between the two groups. In the positive group (9 patients), none was negative with methacholine response and PD(20)-FEV(1) being similar to the bronchial asthma group. The blood total IgE and ECP levels were significantly higher in the positive than in the negative group. CONCLUSION: The characteristics of airway reactivity in chronic asthmatic bronchitis are midway between bronchial asthma and simple chronic bronchitis. The nature of chronic asthmatic bronchitis may be different in different patients.

Adult↗
PubMed1999

[A pathological study on pulmonary fibrosis caused by chronic obstructive pulmonary disease].

OBJECTIVE: To estimate the morphologic characteristics of chronic obstructive pulmonary disease (COPD) induced pulmonary fibrosis. METHODS: 41 autopsy cases of COPD were chosen as the study group and other 10 autopsy cases died from non-respiratory diseases as control group. Histochemical stain and image quantitative analysis were used to identify the type of collagen. RESULTS: In 36 cases (88%) of study group, fibrosis proliferation could be seen in peri-bronchial area, especially in the bronchi under grade VI. Respiratory bronchiole and alveoli were also involved in 11 cases. The main component of collagen in fibrosis area was identified as type III collagen by sirius red stain, reticular fiber could also be seen by Gomori stain. The type III collagen in alveoli distributed interruptedly. Image quantitative analysis showed: the integral optical density (A) of type III collagen was much higher than that of type I collagen in peri-bronchial area (P < 0.01); Both A of type III and type I collagen in peri-bronchial area were higher than that in alveolar area (P < 0.01). Mucous purulent plugs, interrupted elastic fibers in bronchial wall and chronic inflammation accompanied with lymphocytes infiltration in fibrosis area could also be seen. CONCLUSIONS: COPD induced pulmonary fibrosis distributed mainly in peri-bronchial area, especially in the bronchi under grade VI. The main component of proliferative collagen was type III collagen. The genesis of pulmonary fibrosis might be associated with chronic inflammation accompanied with lymphocytes. Peri-bronchial fibrosis, interrupt of elastic fibers in bronchial wall caused the bronchial lumen narrowed or collapsed making both airway obstruction and ventilation insufficiency deteriorated.

Aged↗
PubMed1999

[The tissue oxygenation status and the effects of mechanical ventilation on oxygen dynamics in COPD patients with hypercapnia].

OBJECTIVE: To assess the tissue oxygenation status and the effects of mechanical ventilation (MV) on oxygen dynamics in COPD patient with hypercapnia. METHODS: Gastric intramucosal pH (pHi) measured by gastric tonometer was examined in 19 patients before and after MV. RESULTS: pHi calculated before MV was 7.18 +/- 0.06; After receiving MV, pHi increased gradually until above 7.32 in 4 days; during weaning, there were no significant changes in pHi before and after discontinuation. The correlation analysis showed that there was no correlation between pHi and pHa (r = 0.352, P > 0.05), PgCO(2) and PaCO(2) (r = 0.421, P > 0.05), suggesting that pHi measurement would not be affected by hypercapnia. CONCLUSIONS: Our study showed that tissue oxygenation insufficiency existed in COPD exacerbation stage. Gastric pHi may be used as the parameter to estimate tissue oxygenation status and a predictor of weaning from mechanical ventilation.

Aged↗
PubMed1999

[Studies on the screening high yield acid protease producing strain L336 by combining microwave irradiation with chemical inducing].

A high yield acid protease producing strain Aspergillus usamii L336 was screened from its parent strain B1 by combining microwave irradiation with inducing of nitrosoguanidine and Li2SO4. By incubation of L336 in shaking flask at 31 degrees C for 88 h, its enzyme activity was 5500 u/mL, and its properties for high yield producing acid protease remained stable after many times of subculture and storage for two months.

Aspartic Acid Endopeptidases↗
PubMed1999

[Dynamic changes in the expression of type VI collagen in mice with schistosomal liver fibrosis].

AIM: To clarify the dynamic changes in type VI procollagen mRNA expression and collagen content in mice with schistosomal liver fibrosis. METHODS: Northern blot hybridization and immunohistochemical technique. RESULTS: The expression of alpha 1(VI) procollagen mRNA measured by Northern blot hybridization in the liver of schistosome infected mice was elevated significantly at 8 wk post infection, peaked at 10 wk pi, slightly decreased at 12-16 wk pi, and sustained at high levels until 20 wk pi. Immunohistochemical assay revealed that the staining pattern of type VI collagen first appeared in the walls of central veins and liver sinusoids at 8 wk pi, then extended gradually to the egg granuloma and the portal tract, within and surrounding the egg granuloma to form dense reticular septum at the 12, 16 and 20 wk pi, respectively. The VI collagen content as indicated by the intensity of stain peaked at 16 wk pi. CONCLUSION: Both the expression of alpha 1(VI) procollagen and the content of collagen VI were significantly increased in the liver of mice with early schistosomal fibrosis, suggesting that the type VI collagen detection might be of importance for evaluating the intensity of schistosomal liver fibrosis.

Animals↗
PubMed1999

[An experimental study of viscoelastic properties of articular-cartilage of patella].

OBJECTIVE: To study the viscoelastic properties of human articular cartilages of patella in 8 cadavers, which were obtained from patients ranged in age from nineteen to fifty-one years old and died of acute head injury. METHODS: The creep and stress relaxation of articular cartilage of patella were abserved in the condition of articular cartilage loaded of 500 Pa, just as the physiological load of articular cartilage of patella in keeping human body standing position with the knee joint flexed 30 degrees, during the experiment. The deformation-time curve under certain load, the load-time curve under certain deformity and related data were obtained. RESULTS: Creep and stress relaxation showed that obvious changes occured in the first 10-15 minutes during the whole process. CONCLUSIONS: The data and curve will not only be helpful to understand the pathogenesis of chondromalacia, but also propose a feasible method to study it from biomechanical view, meanwhile provide reference data for designing the prosthesis of patella and material choosing.

Adult↗
PubMed1999

[The surgical treatment of congenital kyphosis].

OBJECTIVE: To summarize the treatment experience of congenital kyphosis, compare the results of several surgical procedures and conclude the lessons of failure cases with surgical treatment. METHODS: Retrospective analysis of 33 patients with surgical treatment of congenital kyphosis. These patients had a follow-up of 6 months to 14 years with an average follow-up of 5 years and 6 months. RESULTS: Among the 33 cases, the average age at surgery was 16.5 years old and the average kyphosis was 90.2 degrees. Four had anterior fusion, and 7 had posterior fusion only, and 22 had combined anterior and posterior fusion. The percentage of correction of the 3 procedures above mentioned was 32.7%, 34.4% and 41.2% respectively. All of the patients with incomplete paraplegia, but 2 had been recovered completely. CONCLUSIONS: The combined anterior and posterior fusion had a better correction than the anterior group or posterior group alone.

Adolescent↗
PubMed1998

Effects of immunotherapy on experimental immunodeficiency-related lymphoproliferative disease.

BACKGROUND: Human lymphokine-activated cells (LAK cells) and interferon alpha (IFN-alpha) have been used clinically in the therapy of posttransplant lymphoproliferative disease (PTLD). However, the efficacy of such therapy has not been extensively tested under controlled experimental conditions. METHODS: A B-cell line, derived from PTLD tissue and clonally related to the parent lesion, was tested for its response to IFN-alpha in vitro. The effects of LAK cells and IFN-alpha therapy were examined in a severe combined immunodeficiency disease (SCID) mouse model in vivo. RESULTS: The PTLD cell line studied showed a 30% decrease in the rate of growth upon incubation with 500 U/ml of IFN-alpha. This in vitro response was also reproduced in vivo, in tumor therapy studies conducted in SCID mice. The magnitude of this inhibitory effect in vivo was a function of tumor burden and dose of IFN-alpha. In parallel experiments, LAK cells reduced the tumorigenicity of a lymphoblastoid cell line derived from the peripheral blood of a patient with PTLD, and prolonged the survival of SCID-beige mice with established lymphoproliferative disease. In contrast with two prior studies, in which the use of autologous cytotoxic T cells was found to be necessary, we found the administration of third-party non-HLA-matched LAK cells also to be effective in reducing tumor burden. CONCLUSIONS: These observations demonstrate the efficacy of immunotherapy for lymphoproliferative disease under controlled experimental conditions, and validate currently ongoing efforts exploring the utility of such therapy in the clinical setting.

Animals↗
PubMed1998

[Human cytomegalovirus infection and congenital malformation].

OBJECTIVE: To study the relationship between intrauterine cytomegalovirus (HCMV) infection and congenital malformation, and to determine the distribution of tissues infected. METHODS: Autopsy samples of 41 infants with congenital malformation and 19 infants with normal appearances were studied. Using polymerase chain reaction (PCR) technique the paraffin embedded specimens of main organs were examined for HCMV infection. In-situ hybridization (ISH) was performed in some of the PCR positive tissues in order to define the distribution of HCMV DNA. RESULTS: 19 of the 41 infants (46.34%) with congenital defects were HCMV positive, while 1 in 19 (5.26%) were positive in the control group, and there was significant difference between the 2 groups (P < 0.05), 20.46% (35/171) of the fetal organ samples were HCMV DNA positive in the malformation group, but only 1 out of 78 samples (1.28%%) was positive in the pulmonary tissue of the control group. More malformations of the digestive system were presented in HCMV infected babies but no statistical significant difference when compared with other systems. Brain tissue had the highest HCMV infection rates (41.37%, 12/29), which was significantly higher than other organs. By ISH technique HCMV DNA was found only in 6 out of 17 PCR positive samples, and they were located at neurons, neurogliocytes, epithelium and interstitial cells of the kidney, and epithelial cells of pulmonary alveolar. CONCLUSION: There are strong correlation between HCMV infection and congenital malformation, and brain is more susceptible to HCMV. By combining PCR and ISH, both sensitivity and distribution of HCMV could be obtained.

Abnormalities, Multiple↗
PubMed1998

A pathological study of in situ thrombosis of small pulmonary arteries and arterioles in autopsy cases of chronic cor pulmonale.

OBJECTIVE: To investigate the incidence of in situ thrombosis of small pulmonary arteries and arterioles during the exacerbation stage of chronic cor pulmonale. METHODS: 49 autopsy cases died from the exacerbation of chronic cor pulmonale were chosen as the study group, while 103 other autopsy cases without chronic cor pulmonale and disseminated intravascular coagulation (DIC) as control group. Morphologic and morphometric studies were taken on lung tissues and other organs with focus on the thrombi in small pulmonary arteries and arterioles. RESULTS: 44 cases of the study group had multiple thrombi in small pulmonary arteries and arterioles, the incidence is 89.8%, and in 9 of them, thrombi in proximal pulmonary arteries co-existed, the incidence is 18.4%. 80% of the thrombi existed in pulmonary arterioles. In the control group, only 3 cases had thrombi in small pulmonary arteries and arterioles, the incidence is 2.9%. All thrombi adhered to endangium, implying that they were in situ thrombi. No intravascular thrombosis was found in other organs. chi 2 test showed that the incidence of thrombosis and the number of thrombi in small pulmonary arteries and arterioles in cases with chronic cor pulmonale were significantly higher than those of the control group (P < 0.01). CONCLUSIONS: Multiple in situ thrombosis in small pulmonary arteries and arterioles is a prominent and common pathological change during the exacerbation stage of chronic cor pulmonale. The study suggests a new diagnostic and therapeutic concept and gives a morphological and theoretical basis for the clinical application of anticoagulants or even the thrombolytic agents for the cases of chronic cor pulmonale in the exacerbation stage.

Aged↗
PubMed1998

[Detection of telomerase activity and its clinical significance in colonic cancers by PCR-ELISA].

Using the recently developed telomerase PCR-ELISA detection kit, the authors detected the telomerase activity in 12 paired colonic cancer biopsies. It was found that strong telomerase activity was detected in all of the 12 colonic cancer tissues but not in adjacent non-cancerous tissues. These results suggest that the detection of telomerase activity by the PCR-ELISA assay could be used as a supplementary means for the diagnosis of colonic cancer.

Biopsy↗
PubMed1998

[The study of platelet function in patients with chronic pulmonary heart disease].

OBJECTIVE: To study the platelet function in patients with chronic pulmonary heart disease. METHOD: Subjects were divided into three groups (cor pulmonale exacerbation, cor pulmonale remission stage and normal control). Platelet adhesion rate (PAdT), platelet aggregation rate(PAgT), alpha-granule membrane protein (GMP-140) and glycoproteinII were measured by radioimmunoassay, flow cytometry and other methods. RESULT: In cor pulmonale exacerbation, the values of PAdT, PAgT, GMP-140 and GPII were31% +/- 16%, 14% +/- 8%, 190 +/- 49 molecule/platelet and 145% +/- 36% respectively. In cor pulmonale remission stage, the values were 33% +/- 13%, 38% +/- 16%, 178 +/- 33 molecule/platelet and 137% +/- 48% respectively. These findings showed that the values of PAdT, GMP-140 and GPII in cor pulmonale exacerbation were markedly increased, whereas PAgT was significantly reduced. However, PAdT, PAgT, GMP-140 and GPII in remission stage were all higher than that of normal group. CONCLUSION: In vivo platelet activation is indeed present in patients with chronic pulmonary heart disease.

Aged↗
PubMed1998

[The correlative study on nitric oxide and cotinine nicotine metabolite in smokers' urine].

OBJECTIVE: In order to observe the changes of nitric oxide (NO) affected by smoking and their relationship in vivo. METHOD: 60 male nonsmoker as the control group, 192 male smoked as the study group which was divided into 4 subgroups (1-10, 11-20, 21-30 and > 30) according to the number of cigarettes smoked per day. The metabolite of nicotine-cotinine in urine was assayed by means of barbituric acid. Cotinine was used as an objective indicator to reflect the smoking quantity. The urinary NO (NO2.-) was determined with colorimetric estimation. RESULT: The urinary cotinine level in the 4 subgroups were 6.6 +/- 1.6, 25.1 +/- 6.2, 37.8 +/- 8.5, 53.6 +/- 8.3 (mumol/L) respectively. The urinary NO of nonsmokers were 4.43 +/- 0.97 (mumol/L), in the 4 subgroups it was 3.04 +/- 0.07, 2.75 +/- 0.05, 2.18 +/- 0.01, 1.60 +/- 0.01 (mumol/L) respectively. The results of statistical analysis between the 4 subgroups were t = 4.2894, P < 0.01; t = 2.7446, P < 0.01; t = 2.7667, P < 0.01 accordingly. As compared between the 4 subgroups of smokers and the nonsmokers, the statistical results were t = 2.7118, P < 0.01; t = 3.1826, P < 0.01; t = 3.2100, P < 0.01; t = 4.6600, P < 0.01; respectively. The statistical differences between the 4 subgroups were t = 2.9286, P < 0.01; t = 3.0357, P < 0.01; t = 3.8181, P < 0.01; t = 2.2160, P < 0.05 respectively. There was a good positive correlation between the number of cigarette smoked and urinary cotinine content (r = 0.8626, P < 0.01), also a significant negative correlation between it and urinary NO level (r = -0.6596, P < 0.05). A negative correlation could be observed between urinary cotinine and NO (r = -0.6026, P < 0.05). CONCLUSION: With the increase of quantity and duration of smoking, the NO content in vivo will decrease, suggested that the vascular endothelial cells could be damaged severely.

Adult↗