Search PubMed⌕ Search

Biomedical subjects

X Qi

Publications and source records attributed to X Qi.

82 records · Page 5Linked to original sources
PubMed1993

Protein tyrosine kinase inhibitors decrease induction of nitric oxide synthase activity in lipopolysaccharide-responsive and lipopolysaccharide-nonresponsive murine macrophages.

We investigated tumoricidal activation and induction of nitric oxide synthase (NOS) activity in macrophages from LPS-responsive (C3H/HeN) and -unresponsive mice (C3H/HeJ). Macrophages were incubated in vitro with a synthetic lipopeptide or with LPS and IFN-gamma. LPS and IFN-gamma activated C3H/HeN but not C3H/HeJ macrophages to lyse B16 melanoma cells. In contrast, lipopeptide and IFN-gamma activated macrophages from both strains of mice. Genistein, a specific inhibitor of protein tyrosine kinase, significantly blocked tumoricidal activation of macrophages from both strains of mice. Genistein did not affect tumor cell binding but significantly inhibited the production of nitric oxide. Genistein, herbimycin A, and tyrphostin inhibited the induction of NOS activity in macrophages from both strains of mice. These data suggest that protein tyrosine kinase activity is involved in the signal transduction pathway of LPS and other synthetic bacterial-related immunomodulators at a point preceding triggering of macrophage tumoricidal activation and expression of inducible NOS activity.

Amino Acid Oxidoreductases↗
PubMed1993

Evidence of L-arginine/nitric oxide pathway in endothelium and smooth muscle of human internal mammary artery.

UNLABELLED: The L-arginine-nitric oxide (NO) pathway was investigated in human internal mammary artery (HIMA) in vitro. HIMA rings were mounted in organ bath, and then incubated in Krebs buffer for 1 to 8 hrs, relaxing agents were tested. Under these conditions, L-arginine (0.1 microM - 1 mM) elicited only minor relaxation after 2 hr incubation, whereas with increased incubation time (4, 6, 8 hrs), the concentration-dependent relaxation to L-arginine increased significantly in endothelium-intact and -denuded vessels. NG-nitro-L-arginine (100 microM) or NG-monomethyl-L-arginine (100 microM) or methylene blue (2.7 microM) partially inhibited L-arginine relaxation. In endothelium-intact HIMA and in both types of rings A23187 (10 microM) and L-arginine (100 microM), respectively, increased the concentration of NO in medium and cGMP content of vascular tissues. These increases were partially inhibited by NG-nitro-L-arginine (100 microM) or methylene blue (2.7 microM). CONCLUSION: in smooth muscle of HIMA L-arginine-NO conversion is calcium independent, which is different from that in endothelium.

Acetylcholine↗
PubMed1993

Tyrosine phosphorylation of mitogen-activated protein kinases is necessary for activation of murine macrophages by natural and synthetic bacterial products.

The purpose of these studies was to determine the intracellular signal transduction pathways of bacterial products in murine macrophages from lipopolysaccharide (LPS)-responder C3H/HeN and LPS-nonresponder C3H/HeJ mice. Both LPS and synthetic lipopeptide CGP 31362 (LPP) induced production of tumor necrosis factor alpha (TNF-alpha) in C3H/HeN macrophages. In C3H/HeJ macrophages, however, TNF-alpha was induced only by incubation with LPP. Both LPS and LPP induced tyrosine phosphorylation on proteins with apparent molecular masses of 39, 41, and 45 kD (p35, p41, and p45) in C3H/HeN macrophages, whereas in C3H/HeJ macrophages, tyrosine phosphorylation was induced only by LPP. 20-h incubation with LPS or LPP downregulated TNF-alpha production/secretion and tyrosine phosphorylation in C3H/HeN macrophages induced by additional LPS or LPP. In C3H/HeJ macrophages, however, the downregulation of TNF-alpha production and tyrosine phosphorylation were observed only with LPP. Protein kinase assays, Western blotting analyses, phenyl-Sepharose chromatography, and immunocomplex kinase assay suggested that p45 and p39 were similar or identical to mitogen-activated protein (MAP) kinase 1 and 2, respectively. Pretreatment of macrophages with LPS or LPP did not change the amount of kinase proteins but inhibited the stimulation of kinase activity by the agents. These data suggest that MAP kinases are among target proteins involved in the transduction of LPS and LPP signals that lead to activation of murine macrophages to produce/secrete TNF.

Amino Acid Sequence↗
PubMed1993

Influence of pulmonary blood flow on gas exchange in piglets.

We previously reported that O2 diffusion was limited in piglets. To test the hypothesis of an inadequacy between diffusion and perfusion in piglets (< 4 wk) vs. older pigs (> 8 wk), we compared in these two age groups the effect of an increase (by opening an arteriovenous fistula) or a decrease (by inflating a balloon in the inferior vena cava) in cardiac output (Q) on gas exchange and on the O2 equilibration coefficient D/Q beta [ratio of the diffusion capacity of O2 (D) to the product of Q and the capacitance coefficient of blood (beta)]. In piglets but not in older pigs, a decrease in Q improved the alveolar-arterial Po2 difference (P < 0.05) and D/Q beta (P < 0.05), whereas an increase in Q had the opposite effect. Changes in the alveolar-arterial O2 difference and D/Q beta were linearly correlated with Q (r = 0.75, P < 0.01 and r = 0.88, P < 0.01, respectively). We suggest that the impaired O2 diffusion in piglets was due to inadequate diffusion-perfusion equilibrium of O2.

Aging↗
PubMed1992

Characterization of the Major Protease Involved in the Soybean beta-Conglycinin Storage Protein Mobilization.

Protease C1, the protease responsible for the initial degradation of the alpha' and alpha subunits of the soybean beta-conglycinin storage protein (Glycine max [L.] Merrill), has been purified. The enzyme was found by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to have a molecular weight of 70,000 and a pH optimum of 3.5 to 4.5. Susceptibility to protease inhibitors indicates that protease C1 is a serine protease. Study of the proteolytic intermediates generated suggests that the cleavage of the alpha' and alpha subunits of beta-conglycinin by protease C1 results in intermediates that are 1 or 2 kilodaltons smaller than the native alpha' and alpha subunits. Following that, a succession of intermediates exhibiting molecular masses of 70.0 and 58.0 kilodaltons, then 63.0, 61.0, 55.0, and 53.5 kilodaltons, are observed. A 50.0- and a 48.0- kilodalton intermediate are the final products of protease C1 action. Comparison of these intermediates with the prominent anti-beta-conglycinin cross-reacting bands that increase during the first few days of germination and early growth show that protease C1 plays an important physiological role, but not an exclusive one, in the living plant.

Journal Article↗
PubMedDate not supplied

Modeling neuronal dynamic coding in primary visual cortex.

The visual system can be considered as a multi-layered and dynamic image processing system. According to experimental evidence, the receptive field (RF) organization is characterized by spatio-temporal properties. The modified extended Gabor (MEG) function model was proposed to describe the main spatio-temporal properties of RF at different levels of visual pathway. Based on the MEG model, a three-layered dynamic coding model was constructed for a complex cell. The responses of the complex cell depend on synaptic events from a simple cell assembly within a time window. The membrane potential evolution equation was applied to the analysis of the length of a time window. The simulation results demonstrated that a complex cell plays as a coincidence detector in encoding synaptic events within the time window.

Mathematics↗