SEM observations of ascaris eggs by freezing cracking technic.
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Biomedical subjects
Publications and source records attributed to X Q Meng.
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The shell of Taenia eggs are easily ruptured and lost if the eggs are prepared according to routine methods for SEM observation. As a result, only the vitelline layer is normally seen. When the uterus is fractured while frozen, the eggs located in the lateral uterine branches are exposed and do contain intact shells. In the fully developed eggs of T. solium, six layers surround the oncosphore. Starting from the outermost structure, they are the egg shell, the vitelline layer, the outer embryonic membrane, the middle embryophoric block layer, the inner basal membrane and the oncosphoral membrane, Numerous lacunae and capillaries from a fine tubular system in he embryophore. The functional significance of these structures is discussed. In addition two structures, which can be used to distinguish eggs of T. solium from those of T. saginatus are presented.
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The fine-structure of the egg shell and the oocyte of A. lumbricoides were examined with SEM. DMSO freeze fracture was used to obtain images of the eggs. Numerous filamentary fibers occur on the outer surface of the protein layer. These fibers are arranged in a network-like structure between the external projections of adjacent eggs. Twelve to 16 mammillated projections appear around the external periphery of each freeze fractured egg. Beneath the projection is a chitinous shell consisting of three layers; the outerlimiting membrane, the middle chitinous zone and the inner limiting membrane. Because the lipoid layer is dissolved by alcohol, it is not revealed with the freeze fracture technique. The oocyte is spherical or subspherical and possesses a continuous unit membrane. Particles, such as dense granules and refringent bodies, occur within the cytoplasm. Occasionally one or two first polar bodies, which will extrude into the perivitelline space, can be seen. The oocyte is frequently surrounded with a layer of deutoplasm.