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Biomedical subjects

X Peng

Publications and source records attributed to X Peng.

At least 127 records · Page 7Linked to original sources
PubMed1998

[Investigation of an Legionnaires' disease outbreak associated with contaminated air-conditioning].

An outbreak of upper respiratory infection (influenza-like syndrome) took place among the staff members of a large office building in Beijing, June, 1997. To understand the cause of the outbreak, with a case-control study with serological antibodies detection against serogroup 1 to 14 of Legionnaires' disease bacteria (LDB) and bacteriological test and culture on samples collected from the air-conditioning system of the office building was performed. The findings showed that the attack rates of tonsillitis and upper respiratory infection were 61.86% and 34.62% respectively. The relation was statistically significant between the symptoms of fever and tonsillitis in the cases (chi 2 = 77.88, P < 0.01). The positive rates of LDB antibodies on serogroups 9, 10, 12, 14 were 45.94% in the cases of upper respiratory infection and 3/15, 6/17, 0/15, 1/16 respectively in the four controls groups. Condensied water samples from an air-conditioning system showed that Legionella Pneumophila was positive. Evidence showed that it was an outbreak resulting from LDB serogroup 9 and 12, accompanied by upper respiratory infection related to air-conditioning system contaminated by LDB. This was the first report Lp infection associated with an air-conditioning system in China.

Air Conditioning↗
PubMed1998

[Effects of early enteral feeding on nitric oxide synthase activity in small intestine in burned rat].

OBJECTIVE: Our previous studies have proved that early enteral feeding could improve intestine blood flow after burn injury. But the mechanism was far from being clarified. This study was attempted to explore the effects of early enteral feeding on nitric oxide synthase (NOS) activity in burned rat small intestine and the relationship between the intestine mucosa blood flow (IMBF) and the activity of NOS. METHODS: The rats were randomly divided into three groups: burned control (B), burned and early enteral feeding (EF), and normal control (C). The activity of NOS including constitute NOS(cNOS) and inducible NOS(iNOS), and the IMBF were determined at postburn 0, 3, 6, 12, 24, 48 hours. RESULTS: It was found that cNOS activity and IMBF were decreased markedly postburn, and there was positive correlation between cNOS and IMBF (r = 0.97, P < 0.01). But the activity of iNOS, total NOS were increased significantly postburn, they had no correlation to the IMBF. In EF group cNOS activity and the IMBF were significantly higher, the iNOS was obviously lower than that of B group and there was no significant difference of total activity of NOS between two groups. CONCLUSION: Our results suggested that NOS which is catalyzed from cNOS may play main role in adjusting IMBF. By using early enteral feeding the activity of cNOS is increased and the ischemic state in small intestine is improved after burn injury.

Animals↗
PubMed1998

[Morphologic changes of red blood cells in various conditions under scanning electron microscope].

To study the changes of RBC in various conditions, blood clots elapsed 6 days in water and air and bloodstains stored in variant time were observed under Scanning Electron Microscope (SEM). The results showed that the morphology of RBC elapsed 6 days in water and air seemed to retain their integrity. They were measured 5, 48-5, 88 microns in diameter, whereas the RBC in the bloodstains stored 14 years were obviously disfigured and some appeared membrane cracking. However, the structure of RBC membrane was well recognized under the SEM. The morphological changes under the SEM and the forensic significance of the RBC were discussed.

Blood Stains↗
PubMed1998

[Observations on rat's muscle at various postmortem intervals by scanning electron microscopy].

The aim of this study was to observe the morphological changes of muscle in the process of rigor mortis. The quadriceps of 40 rats at various postmortem intervals were observed under the scanning electron microscope (SEM) and the light microscope by phosphtungstic acid-haematoxylin staining. The results showed that the striations of muscle were blurred within 4 hours, but they became apparent from 6 hours to 24 hours after death. The authors suggest that this phenomenon be associated with the increased resistance of muscle against the postmortal changes. The observations by scanning electron microscopy and light microscopy have revealed that the muscles do contract in the process of rigor mortis because the distance between two Z lines shortens and the I band narrows, compared with those in anaesthetised animals. The basic biochemical process for the formation of rigor mortis is the same as that of muscle contraction except that the former happens postmortem and the latter antemortem.

Animals↗
PubMed1998

[Polymerase chain reaction associated with dig-labelled DNA hybridization of mip gene to identify new isolated Legionella pneumophila strains].

OBJECTIVE: By testing mip gene, one highly conservative virulence gene of Legionella pneumophila (Lp), as targets of polymerase chain reaction (PCR) and Digoxin-labelled probe hybridization to establish a rapid, specific and sensitive gene analysis method that can identify new isolated suspected bacteria strains as Lp. METHOD: Abstracting all the CDC reference Lp strains' and none-Lp Legionella strains' DNA, as well as the domestic isolated Lp strains' and some non-Legionella control bacteria's DNA as templates, then were processed. All the positive PCR products(if no PCR amplicons were available, the original whole DNA should be detected) were hybridized with Digoxingen-labelled mip gene probe in dot-blot procedure. RESULT: All the tested Lp strains had positive PCR and hybridization results, at the same time, all none-Legionella bacteria and none-Lp Legionella strains got negative results. 6 of 26 isolates were identified as Lp strains by this method. CONCLUSION: Such a Lp strain identification procedure shows high specificity and sensitivity (nearly 100%, in this study), and mean-while can be completed in a relative short time. Surely, this method can be largely available and has a potential value in diagnosis of clinical Legionellosis cases and pursuing the pathogen during Legionnaires' disease outbreaks.

Bacterial Proteins↗
PubMed1998

[Analysis of the major components in cannabis by capillary gas chromatography].

A capillary GC method for the simultaneous determination of cannabidiol (CBD), tetrahydrocannabinol (THC), and cannabinol (CBN) in cannabis is described. The major components in cannabis were extracted with chloroform. After being concentrated by evaporation, the residue was dissolved in methanol. The resulting solution was used for analysis. The conditions for determination were: FID detector, HP-5 capillary column, 10 m x 0.53 mm x 2.65 microns film, column temperature 220 degrees C. The recoveries of the three major components were 97.3%-104.0%(CBD), 97.3%-106.6%(THC) and 95.3%-102.4% (CBN). The coefficients of variation are less than 3%. The limit of detection for the three components was 0.2 mg/L, and their linearity was in the range of 20-120 mg/L. Identification of each component was achieved by comparison of retention times with the standards. This method has the advantages of simplicity, rapidity, sensitivity and accuracy.

Cannabidiol↗
PubMed1998

A brief history of diagnostic cytology.

Being a discipline specialized for diagnosis of tumors, diagnostic cytology has a history of over 100 years. By exploring the origin, development and its formative process, valuable and special information which are helpful to the general investigation of tumor cases and screening of such patients can be provided.

Cell Biology↗
PubMed1998

[Application of diode laser in the operation of retinal diseases].

OBJECTIVES: To evaluate the effects of diode laser photocoagulation applied on 43 cases of various kinds of fundus diseases with media opacity and on 40 cases during vitreo-retinal surgery. METHODS: Indirect ophthalmoscope laser photocoagulator was used for fundus diseases, and endolaser used during surgery. RESULTS: Vision was improved in 24.7% of the eyes, and was unchanged in 62.9% of the eyes. The neovascularization in proliferative diabetic retinopathy regressed in 78.3% of the eyes. All the retinal holes were closed. CONCLUSIONS: Diode laser can be used for treatment of some retinal diseases and in the operation. Good results can be obtained in the eyes with some extent of media opacity. No obvious side-effect was observed.

Adolescent↗
PubMed1998

[Cross-protection against Schistosoma japonicum infection in mice immunized with Trichinella spiralis muscle larva antigen].

AIM: To explore the cross-protection against S. japonicum cercaria challenge in mice immunized with T. spiralis larva antigen. METHODS: Groups of BALB/c mice were immunized with 4 preparations of T. spiralis larva antigen, respectively. Vaccinated mice were then challenged with 30 and 100 S. japonicum cercariae, respectively. Forty-five days later, all mice were sacrificed and examined for adult worm burden, liver and stool egg count. RESULTS: All groups of mice immunized with 4 preparations of T. spiralis larvae antigen without adjuvant showed significant protection against S. japonicum. Among these antigen preparations, the soluble antigen (TsLSA) presented highest protection with reductions in worm burden and liver and stool egg counts of 21.3%, 48.0% and 58.5%, respectively. When mice were immunized with TsLSA plus Freunds complete adjuvant (FCA) or with double-dose of the antigen, the worm reduction rate was increased to 29.3% and 39.6%, respectively. CONCLUSION: Different preparations of T. spiralis muscle larva antigen could induce protection against S. japonicum in mice.

Animals↗
PubMed1997

Ligation of CD2 provides a strong helper signal for the production of the type 2 cytokines interleukin-4 and -5 by memory T cells.

In this study, we investigated the efficiency of costimulatory signaling provided by anti-CD2 monoclonal antibodies (mAbs) for the production of type 1 (IL-2 and IFN-gamma) and type 2 (IL-4, IL-5, and IL-10) cytokines by human T cells. We cultured purified human T cells (freshly isolated from blood) with immobilized anti-CD3 mAb, either alone or in combination with anti-CD28 or anti-CD2 mAbs. When compared with the standard costimulatory signal anti-CD28, anti-CD2 mAbs (9-1 plus 9.6) were shown to be more potent costimulators of IL-4 production and to have similar activity for IL-5 production, but to be less potent for costimulation of IL-2, IL-10, and IFN-gamma production. IL-4 production was completely inhibited by cyclosporin A or by blocking IL-2 activity and its receptor. IL-4 and IL-5 were produced by CD45RO+ T cells but not by CD45RA+ T cells, indicating that anti-CD2 in this system costimulated type 2 cytokine production by differentiated memory cells. In the presence of IL-12, the cytokine profile was shifted to high IFN-gamma and IL-10 production and IL-4 and IL-5 production were slightly inhibited. Our data thus suggest that CD2 ligation plays an important role in the upregulation of Th2-like T cell activity (especially IL-4 production), but they also show that this effect is strongly modulated by IL-12, resulting in predominant IL-10 and IFN-gamma production instead.

Adult↗
PubMed1997

Preparation and Organized Assembly of Nanoparticulate TiO2-Stearate Alternating Langmuir-Blodgett Films

Nanoparticulate TiO2-stearate Langmuir-Blodgett-type monolayers and multilayers were directly obtained by using TiO2 hydrosol as the subphase. The surface pressure-versus-surface area isotherms showed that the monolayer could be compressed to a mean molecular area of 0.25 nm2. The monolayer was transferred onto a CaF2 or Si substrate at a dipping speed of 18 cm/min and surface pressure of 25 mN/m. It exhibited Y-type multilayer films over the range investigated (1-30 layers) with a transfer ratio of 1.0 &plusmn; 0.1. FTIR transmission spectra and linear infrared dichroic spectra provided evidence of the formation of a stable organic/inorganic alternating multilayer with nanoparticulate TiO2 between the organic monolayers. There is only slight absorption in the range of the C;equals;O stretching vibration band of the carboxylic group with the 3- and 6-nm TiO2 nanoparticles. This means that TiO2 particles are packed closely; i.e., the surface coverage is very high. A possible structure consists of layers of nanoparticles in close-packed form with two stearate ion layers inbetween. But it can be seen that there are some carboxylic groups (1700 cm-1) not connected to the 20-nm TiO2 nanoparticle. Electron microscopic (TEM) images of TiO2-stearate monolayers show that high-coverage monolayers were obtained when the surface pressure increased to 25 mN/m and the dipping speed to 18 cm/min. Copyright 1997Academic Press

Journal Article↗
PubMed1997

Quantitative description of analyte migration behavior based on dynamic complexation in capillary electrophoresis with one or more additives.

A comprehensive theory is proposed to describe the migration behavior of analytes in capillary electrophoresis (CE) when one or more additives are present in the buffer solution. This theory amalgamates and extends the previous work done by others. The capacity factor (k') in this theory is defined as the product of the equilibrium constant and the additive concentration, thus, k' changes linearly with additive concentration. The net electrophoretic mobility of an analyte is a function of k', therefore, it can be changed by varying the additive concentration. Three parameters are needed to predict the mobility of an analyte in a one-additive CE system: the mobility of the free analyte, the mobility of the complex, and the equilibrium constant for the analyte-additive interaction (which determines the fraction of the free analyte at different additive concentrations). When additives are used, the change in viscosity obscures this relationship, therefore, a viscosity correction factor is required to convert all mobilities to an ideal state where the viscosity remains constant. The migration behavior of an analyte in a solution with multiple additives can be predicted and controlled, once the equilibrium constants of the interactions between the analyte and each of the additives are obtained separately. beta-Cyclodextrin and hydroxypropyl-beta-cyclodextrin are used as additives and the migration behavior of phenol, p-nitrophenol, and benzoic acid are studied as a model system to verify this theory. When the necessary viscosity correction factor is included, the net electrophoretic mobilities of the analytes obtained from experimental results agree with the values predicted by the theory based on dynamic complexation. Although only experiments with one and two additives were carried out to verify the theory, the equations apply to situations when more than two additives are used. The relationship between the theories of electrophoresis and chromatography is clarified.

2-Hydroxypropyl-beta-cyclodextrin↗
PubMed1997

Redefining the separation factor: a potential pathway to a unified separation science.

Understanding the separation process in capillary electrophoresis (CE) leads to the unification of the theories for separation science. While the separation of analytes is governed by equilibria in chromatography, and by (centrifugal) field in ultracentrifugation, the separation in CE is governed by both equilibria and (electric) field. Therefore, a comprehensive separation theory that describes the separation process of analytes in CE should be able to describe the separation processes in both chromatography and ultracentrifugation. In this paper, we propose that individual capacity factors for each analyte species be used to describe the migration behavior of an analyte. The effect of field on each analyte species, as well as the effect of equilibria are considered in deriving a generalized equation that is applicable for all separation techniques. The separation factor defined at present does not directly relate to the migration rates of the analytes, and therefore can not be used in a generalized theory. We propose that the ratio of the migration rates of a pair of analytes (gamma) should be used as the separation factor, instead of the ratio of the two capacity factors. When gamma is used to describe the separation of two closely migrating analytes, all separation techniques have the same resolution equation.

Chromatography↗
PubMed1997

Interleukin 12 and B7/CD28 interaction synergistically upregulate interleukin 10 production by human T cells.

Interleukin 12 (IL-12) is a heterodimeric cytokine which promotes the development of Th1 cells and their interferon gamma (IFN-gamma) production after TCR/CD3 triggering. Previous reports indicate that IL-12 synergizes with accessory signalling through B7/CD28 interaction in inducing proliferation and IFN-gamma production by human T cells. In this study, we investigated the capacity of IL-12 to modify cytokine synthesis by freshly purified human peripheral blood T cells stimulated with anti-CD3 as the primary signal and with CD80 on transfected mouse cells as an accessory signal. Our data demonstrate that IL-12 indeed synergizes with B7/CD28 interaction, not only in inducing IFN-gamma production, but also in enhancing IL-10 synthesis in a dose-dependent manner. In contrast, IL-4 and IL-5 production were slightly inhibited by IL-12. The effect of IL-12 on the secretion of IL-10 was confirmed by stimulating T cells in the absence of accessory cells with immobilized anti-CD3 mAb and soluble anti-CD28 mAb. CD80 and IL-12 mainly costimulated CD4+CD45RO+ T cells but not CD8+ or CD45RA+ T cells to produce IL-10. Cyclosporin A (CsA) partially inhibited, and a neutralizing anti-IL-2 mAb in combination with anti-IL-2R mAbs (anti-Tac and Mik beta1) strongly reduced IL-10 production. On the other hand, IL-12 did not affect IL-2 production. The data thus suggest a model in which optimal IL-10 production by stimulated peripheral blood T cells results from the co-operation of IL-12, B7/CD28 interaction and the ensuing IL-2 activity.

Adult↗
PubMed1997

Life events and anxiety in Chinese medical students.

The authors studied a sample of 537 Chinese medical students aged 15-21 years using Zung's Self-Rating Anxiety Scale (SAS) and a life events checklist. The results showed that test pressure, less free time, peer competition, failure in a test, and financial problems were the most common stressful experiences for medical students during the previous 12 months. Social and personal problems were rated the highest on scores of perceived stress. Of all students, 12.5% scored over the cut-off point on the SAS previously established to indicate risk of psychiatric disorder. Using a stepwise regression analysis, it was shown that poor health status, little physical exercise, financial problems in the family, test pressure, conflict with classmates, the personality trait of introversion, getting up late in the morning, and freshman status were independently associated with the presence of anxiety.

Adolescent↗
PubMed1997

Chronic nicotine treatment up-regulates alpha3 and alpha7 acetylcholine receptor subtypes expressed by the human neuroblastoma cell line SH-SY5Y.

Chronic exposure to nicotine has been reported to increase the number of nicotinic acetylcholine receptors (AChRs) in brain. The mechanism of up-regulation for the alpha4beta2 AChR subtype, which accounts for the majority of high affinity nicotine binding in mammalian brain, has previously been shown to involve a decrease in the rate of alpha4beta2 AChR turnover. Here, we report an investigation of the extent and mechanism of nicotine-induced up-regulation of alpha3 AChRs and alpha7 AChR subtypes expressed in the human neuroblastoma cell line SH-SY5Y. Up-regulation of human alpha3 AChRs and alpha7 AChRs, unlike alpha4beta2 AChRs, requires much higher nicotine concentrations than are encountered in smokers; the extent of increase of surface AChRs is much less; and the mechanisms of up-regulation are different than with alpha4beta2 AChRs. The mechanisms of up-regulation may be different for alpha3 AChRs or alpha7 AChRs. Chronic treatment with nicotine or carbamylcholine, but not d-tubocurarine, mecamylamine, or dihydro-beta-erythroidine, induced a 500-600% increase in the number of alpha3 AChRs but only a 30% increase in alpha7 AChRs. Chronic nicotine treatment did not increase affinity for nicotine or increase the amount of RNA for alpha3 or alpha7 subunits. The effect of nicotine on up-regulation of alpha7 AChRs was partially blocked by either d-tubocurarine or mecamylamine. The effect of nicotine treatment on the number of alpha3 AChRs was only slightly blocked by the antagonists d-tubocurarine, mecamylamine, or dihydro-beta-erythroidine at concentrations that efficiently block alpha3 AChR function. Most of the nicotine-induced increase in alpha3 AChRs was found to be intracellular. The alpha3 AChRs, which accumulate intracellularly, were shown to have been previously exposed on the cell surface by their susceptibility to antigenic modulation. The data suggest that chronic exposure to nicotine may induce a conformation of cell surface alpha3 AChRs that at least in this cell line are consequently internalized but not immediately destroyed.

Endocytosis↗
PubMed1997

[Cross reaction of Trichinella spiralis larva antigens with sera of schistosomiasis japonica].

Cross reactions of T. spiralis muscular larva antigens (TsMLA) against several sera from patients with other parasitic diseases by ELIB (enzyme-linked immunoblotting technique) were studied. The proteins of 31-100 KDa were recognized by sera of two cases of acute schistosomiasis, while proteins of 44/45, 51/53, 62/64 and 100 KDa were recognized by sera of 11 cases of chronic schistosomiasis. The proteins of 60 KDa showed varied cross reactions against sera of five kinds of patients with other parasitic diseases (filariasis, hookworm diseases, paragonimiasis, ascariasis and clonorchiasis). Only the proteins of 45 KDa were recognized by six of fifteen cases of normal human sera. All of > 29 KDa proteins were recognized by sera of rabbit infected by T. spiralis. It is suggested that there were many antigens in Trichinellen muscular larva which shared in common with several other parasites. The cross reactions of TsMLA against Schistosoma japonicum may be differentiated on the positive reaction bands of 44/45, 51/53, 62/64 and 100 KDa proteins which was specific to the chronic schistosomiasis using the TsMLA by means of ELIB.

Animals↗