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Biomedical subjects

X Liu

Publications and source records attributed to X Liu.

At least 901 records · Page 50Linked to original sources
PubMed1999

[Partial splenic embolotherapy in hypersplenism with portal hypertension].

Twenty-one cases of hypersplenism with portal hypertension were treated with partial splenic embolization. The results were that spleens were shrink and the condition of white blood cells, platelets, and prothrombin time of the patients were obviously improved. We conclude that the appropriate antibiotics, superselective catheterization, and the management of postoperation are necessary; the volume of embolism is limited from 50% to 75% of the spleen. This technique has the features of steady therapeutic effect, safety, and microtrauma.

Embolization, Therapeutic↗
PubMed1999

[Preliminary study on apoptosis and expression of apoptosis-related genes in peripheral blood lymphocytes of patients with systemic lupus erythematosus].

This study was conducted to explore the clinical implication of the relationship between systemic lupus erythematosus (SLE) and peripheral blood lymphocytes (PBL) apoptosis and apoptosis-related gene expression. Terminal deoxynucleotidyl transferase mediated nick end labelling technique and flow cytometry were used to detect the apoptotic cell; immunohistochemistry ABC was used to detect the expressions of bcl-2 and Fas in 29 cases of SLE. The results showed that the rate of apoptosis of PBL in SLE patients was significantly higher than that in normal control (P < 0.001), and that in patients with rheumatoid arthritis (P < 0.001). After 48 hour's culture, the apoptotic rate further increased in SLE patients (33.44%). There was a significant correlation between the SLE activity and the rate of apoptosis in vitro (r = 0.86, P < 0.001). The rate of apoptosis of PBL in SLE-nephritis patients was higher than that in non-SLE-nephritis patients (P < 0.01). Before and after the culture, the expressions of bcl-2 and Fas in PBL of patients with SLE were higher than those in normal control (P < 0.01, P < 0.001). So the authors suggest that increased rate of apoptosis may be used as an index for evaluating the severity of SLE cases.

Adolescent↗
PubMed1999

[Effect of arginine on deep partial-thickness burn in rats].

To investigate the therapeutic effect of arginine on burn, a randomized controlled trial in 20% TBSA deep partial-thickness burned rats was designed. The rats' wound healing, cellular immunity and nitrogen balance were evaluated at appointed postburn days. The results showed that the arginine group got better wound healing and faster recovery of cellular immunity, and achieved positive nitrogen balance. These suggest that supplement of exogenous arginine after burn may be beneficial to the victim in promoting would healing, reducing postburn immune suppression and enhancing anabolism of protein.

Animals↗
PubMed1999

[Mesothelioma in rats following-intrapleural-injection of crocidolite].

In order to investigate the potential of pleural mesothelioma induced by crocidolite, author collected samples from four county areas (Dayao, Yaoan, Muding and Yanyuan) in China. A suspension of 1 ml containing 20 mg crocidolite fiber was injected into the right pleural cavity of each rat in various test groups and UICC crocidolite group, totally 40 mg. The negative control was given saline. The results showed that the incidence of mesothelioma was 56.0%-68.8% (in which the rate of induction in Yanyuan group was the highest), and the survival time of the first case of mesothelioma was 273-347 days in the test groups, the Yanyuan group had shorter latency, and the mean survival days of the rats with mesothelioma for four groups were 560, 490, 593 and 498 days respectively; the shortest mean survival time was that for Yanyuan group. The major histological type of mesothelioma induced by crocidolite was the fibrous type. The degree of differentiation in all mesotheliomas was mainly intermediate and low. The results suggested that when rats were intrapleurally inoculated with samples of crocidolite and saline, an appreciable proportion of animals developed mesothelioma except those of the saline group. Compared with other groups, the Yanyuan group had stronger potential of crocidolite-induced mesothelioma.

Animals↗
PubMed1999

[Role of sera from peptic ulcer patients in cell proliferation of human gastric carcinoma].

This study aimed at the relationship of helicobacter pylori infection to gastric carcinoma pathogenesis. Forty-nine serum samples from patients with gastric and duodenal ulcer and 20 serum sample from normal subjects were collected for the assessment of serum gastrin by radioimmunoassay (RIA) and serum IgG, IgA and IgM antibodies against H. pylori by ELISA respectively. The patients, serum samples were divided into four groups for observations on whether the sera would affect the cell proliferation of HAC803 derived from human gastric carcinoma and of HT29 from human colonic carcinoma cultured in vitro. The cell proliferation was determined by MIT assay. The results showed that the level of serogastrin in gastric and duodenal ulcer was significantly higher than that in normal control (139.6 +/- 38.17 pg/ml vs 63.12 +/- 28.7 pg/ml, P < 0.01). As for the sera of ulcer subjects, the highest anti-HpIgG group (group A) and the highest anti-Hp IgA group (group B) displayed a significantly enhanced cell proliferation of human gastric carcinoma (P < 0.05), especially group B. But all the four groups had less significant increase in cell proliferation of human colonic carcinoma (P > 0.05). These findings indicate that, in the highest anti-HpIgG group and the highest IgA group of the peptic ulcer subjects infected by H. pylori, the sera can enhance cell proliferation of gastric carcinoma, however the role is less associated with serum gastrin.

Antibodies, Bacterial↗
PubMed1999

[Biological characteristics and damaging regularities of grubs endangering the growth of Ophiopogen japonicus (Thunb.) Ker-Gawl].

OBJECTIVE: To provide a scientific basis for prevention and control of the damage done to Ophiopogen japonicus by grubs. METHOD: Conducting laboratory observation along with field investigation. RESULT: The grubs endangering the growth of O. japonicus are mainly Hoplosternus incanus, Holotrichina paraullela and Anomala corpulenta, H. incanus takes two years to make a generation and overwinters in the form of second or third-instar larvae. H. parallela and A. corpulenta generate yearly and overwinter in the form of third-instar larvae. The extent of the damage done to O. japonicus by grubs is related to such factors as the number of sequential cropping years, the model of intercropping and the type of soil. CONCLUSION: Prevention and control of the pest grub should be based primarily on proper agricultural (cultivating) measures.

Animals↗
PubMed1999

[Biological characters of adenoid cystic carcinoma cells transduced with HSV-tk gene].

OBJECTIVE: To study the effects of herpes simplex virus thymidine kinase(HSV-tk) gene transduction on adenoid cystic carcinoma(ACC) cells. METHODS: Gene transduced cells were detected with growth rate, scanning electronic microscope, transmission electronic microscope, cell cycle analysis and the expression of major histocompatibility complex(MHC) class I or II molecules. RESULTS: The parameters detected in gene transduced cells and parental tumor cells were similar. CONCLUSION: The HSV-tk gene transduction would not change the biological characters of tumor cells greatly.

Carcinoma, Adenoid Cystic↗
PubMed1999

[Rat submandibular gland tumor induced by 3-methylcholanthrene].

OBJECTIVE: To study the genesis and the development of salivary gland tumor (SGT). METHODS: SGT animal models were established by injection of 3-methylcholanthrene (3-MCA) oil solution into 40 submandibular glands of Sprague-Dawley (SD) rats, then histopathology and ultrastructure of induced tumors were observed under microscope, and immunohistochemical detection of keratin and actin was carried out. RESULTS: 32 models of submandibular gland tumors were induced successfully, with 4 rats of squamous cell carcinoma and 28 leiomyosarcoma, and the carcinogenesis of 3-MCA and tissue genesis of induced tumors were discussed. CONCLUSION: Submandibular gland tumor can be induced by 3-MCA, and sarcoma was of high incidence.

Animals↗
PubMed1999

[Preparation and application of a new chloromethylstyrene encapsulated silica single anion column].

The silanized silica was firstly encapsulated with chloromethylstyrene and then quaternized by N, N-dimethylbenzylamine. Thus a new kind of strong anion stationary phase was prepared. This strong anion silica column has superior separation ability for the anions by using indirect ultraviolet detection with wavelength at 254 nm and the mobile phase of V(methanol):V(0.125 mol/L NaH2PO4) = 60:40. Moreover, separation of mono- and di-petroleum sulfonates in Yumen sample could also be good with gradient elution. The sample was first eluted with 100% A [V(methanol):V(water) = 60:40] for 1 min, then the eluent was changed linearly to 100% B [V(0.125 mol/L sodium phosphate monobasic):V(methanol) = 60:40] within 1 min, then the sample was continuously eluted with 100% B for 6 min.

Chromatography, High Pressure Liquid↗
PubMed1999

[Differentiation of cultural endothelial cells].

The disparity of function and ultrastructural characteristics between cells in vivo and cultural cells in vitro has been attributed to the differentiation of cells, so differentiation is the key to detecting the differences between endothelial cells (ECs). The ECs are widely distributed over the entire inner surface of blood vessels in the body and exhibit noticeable heterogeneity. The cultural ECs also show heterogeneity. This is a literature review focusing on the recent advance in researches on the embryonic origin of ECs, the heterogeneity of ECs and the factors that influence differentiation of ECs. Also described is the prospect of studies aimed at elucidating the molecular mechanisms involved in the differentiation of ECs. It is hoped that future studies will enhance the realization of oriented differentiation of ECs.

Cell Differentiation↗
PubMed1999

[Simulation on the hydrodynamics of a flow chamber system].

The distributions of the velocity and the shear stress of flow chamber are achieved by using the nondimensional technique and the method of Bessel function of imaginary argument according to Navier-Stokes' equations. When reduced to the representation of steady flows, the formulae obtained in this paper are consistent with Cao's theoretical formulae and their experimental results.

Computer Simulation↗
PubMed1999

[Study on correlation between in vivo and in vitro tests of biomaterials--correlation between leukocyte chemotaxis test and muscular implant test in rabbits].

The purpose of this study was to investigate the correlation between the results of in vivo and in vitro tests of biomaterials. With muscular implant test and leukocyte chemotaxis test, the levels of tissue toxicity of seven biomaterials were evaluated. The samples with high-level toxicity showed weak leukocyte chemotaxis, which would influence the anti-inflammatory reaction to foreign bodies. By the method of Spearman rank correlation, the coefficient was between 0.89286-0.98198 indicating that the correlation of the two tests is excellent.

Animals↗
PubMed1999

[The advances of research for problems of developing flow in blood vessel].

The development and the tendency of the problems of developing flow in the vessel are reviewed in this paper. It is pointed out that the problems of developing flow of viscoelastic blood vessel are front subjects, and that consideration shouldbe be give to three aspects, namely the blood flow, the blood vessel's wall motion and the coupling between the blood and the wall.

Blood Flow Velocity↗
PubMed1999

[Cloning of glycoprotein B gene from strain Rispens of Marek's disease virus and construction of recombinant fowlpox virus].

Purified DNAs from Chicken Embryo Fibroblast (CEF) cultures infected with MDV strain Rispens were used as templates. Specific fragment with the size of about 2.9 kb was successfully amplified through Polymerase Chain Reaction(PCR) and identified to be gB gene of MDV by dot blot hybridization with a digoxigenin-labelled MDV gB specific oligonucleotide probe. The gB gene from strain Rispens was cloned into pUC19 and FPV insertion vector pFG1175-1 to construct plasmid pMGB and pFGBR1775-1 respectively. DOSPER liposome-mediated transfection with insertion vector DNA pFGBR1175-1 was performed on CEF monolayers infected with FPV 3-4 h earlier. Recombinant FPV was clone purified. Immunofluorescence Assay(IFA) showed that MDV gB gene had been expressed in FPV.

Animals↗
PubMed1999

[The outer membrane protein (OMP) patterns of Escherichia coli isolates of predominant O serogroups originated from chichens in different regions in China].

The purpose of this study is to determine the outer membrane protein (OMP) patterns of avian Escherichia coli isolates with predominant serogroups originated from 18 provinces, autonomous regions and municipal cities in China. Total of 204 isolates belonging to O18, O78, O2, O88, O11 and O26 serogroups respectively, were tested. The outer membrane proteins of these isolates were isolated with the improved N-lauroylsarcosine method and analyzed by SDS-PAGE. 4 different OMP patterns were identified with these isolates of predominant serogroups. 3 different OMP patterns with 56 isolates of O18, 4 with 54 isolates of O78, 2 with 28 isolates O2, 1 with 26 isolates of O88, 3 with 22 isolates of O11 and 1 with 18 isolates of O26 respectively, were found. Isolates with OMP pattern 1 were discovered in 6 different serogroups, and OMP-3 pattern was also shared by O18, O78 and O11 serogroups isolates. These results indicated that the OMP patterns of avian pathogenic Escherichia coli isolates of O18, O78, O2, O11 serogroups which were isolated from different regions in China were heterogeneous, and all of O88 and O26 serogroups isolates just belonged to OMP pattern 1. Moreover, the OMP pattern 1 was presented in isolates of six different predominant O serogroups.

Animals↗
PubMed1999

[The isolation and characterization of type 1 pili from pathogenic Escherichia coli of chicken origin].

The pili from pathogenic Escherichia coli isolates 566, 1794 and TK3 of chicken and turkey origin were purified. After mechanic detachment from the bacterial cells, the pili were concentrated by precipitation with ammonium sulfate, dialyzed, and solubilized in buffer containing deoxycholate. The fraction containing the pilus was purified further by ultracentrifugation in a sucrose gradient. After ultracentrifugation, the pili at the density of 1.10 to 1.15 g.cm-3 (between 10%-20% of sucrose gradients) were collected, and the purified pili from strain 566, 1794 and TK3 had an apparent molecular weight of 17,500, 17,000 and 17,000 respectively, which retained their ability to bind the erythrocyte in a mannose-inhibitable fashion. Hyperimmunesera raised in BALB/C mice against the purified pili from strain 1794 reacted positively with type 1 pili from both isolates 566 and TK3 by immuno blot. These results revealed that the three strains either Chinese or north american isolates expressed type 1 pili which had molecular weights from 17,000 to 17,500, and they have common antigenic epitopes.

Animals↗