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Biomedical subjects

X Liang

Publications and source records attributed to X Liang.

At least 253 records · Page 14Linked to original sources
PubMed1991

Detection of HPV-16 DNA in cervical carcinoma by paraffin section in situ hybridization.

Thirty-eight cervical carcinomas and one cervical condyloma were examined for the presence of human papillomavirus type 16 (HPV-16) DNA using 3H-dcTP-labelled HPV-16 DNA probe in paraffin section in situ hybridization and dot blot hybridization (Tm-17 degrees C). The results showed that HPV-16 DNA positive rate in our series was about 74.4% (29/39) as determined by paraffin section in situ hybridization and about 71.8% (28/29) by dot blot hybridization. There was no significant difference between the two methods. We thus confirmed that paraffin section in situ hybridization is an informative, reliable, and sensitive method for the diagnosis of cervical HPV infection.

Condylomata Acuminata↗
PubMed1991

The association between cervical carcinoma and human papilloma virus (HPV) in Xiangyuan County.

A mass survey was conducted to investigate the association between cervical carcinoma and human papilloma virus (HPV) infection in a high-risk area, Xiangyuan County, Shanxi Province. Fifty-four cases of cervical cancer in situ (CIS), including severe dysplasia (CIN III), 14 cases of invasive cervical carcinoma, 28 cases of mild cervical dysplasia (CIN I), and 13 cases of moderate cervical dysplasia (CIN II) were identified among 6710 women examined. One hundred and sixty-nine punch biopsies from abnormal cervix and genital tract were examined for the presence of HPV 6B/11, 16 and 18 DNA sequences by dot blot hybridization: The positive rates of HPV infection in cervicitis, CIN I, II, III, CIS and invasive cervical carcinoma specimens were 35.06% (27/77), 25% (2/8), 33.33% (2/6), 40% (2/5), 70.58% (24/34) and 40% (4/10), respectively. Among these groups, there was no regular distribution of HPV types except for HPV 16, the positive rate of which was increased from 25% in CIN I to 46.15% in CIS: HPV 16 was also the only type seen in the invasive cervical cancer group. Distribution analysis of HPV types in HPV-positive cervical cancer tissues uncovered an HPV 16 positive rate of 59.4%; 3.7 and 2.4 times higher than those of HPV 6B/11 and HPV 18, respectively. These data confirm the close association between cervical cancer and HPV 16 infection.

Adult↗
PubMed1991

[Isolation of bilirubin oxidase from Myrothecium verrucaria and the optimum conditions of enzyme production].

A bilirubin oxidase (EC 1.3.3.5) producing strain, Mv 2.1089, was isolated from several strains of Myrothecium verrucaria by dilution method. The optimum conditions of enzyme production were investigated and the results were as follows: the suitable medium was cultured at 25 degrees C on a rotating shaker glucose and peptone, at pH 6.0. The strain was cultured at 25 degrees C on a rotating shaker (150 r/min) for 96 h. Bilirubin oxidase with 0.5-1.5 u/ml was obtained in the culture medium.

Mitosporic Fungi↗
PubMed1991

[Research on the relationship between central acetylcholine and acupuncture analgesia].

The article systematically reported our physiological and biochemical work on the relationship between central acetylcholine and acupuncture analgesia. The results indicate: 1. Acupuncture on acupoints could produce analgesia. 2. Ach contents in cerebrospinal fluids and brain increased under acupuncture analgesia. 3. AChE activities iu brain elevated under acupuncture analgesia. 4. ChE inhibitor reinforced the effects of acupuncture and inhibitor of ACh synthesis could inhibit the effects of acupuncture, which could be reversed by administration of ACh and chlorocholine. M-AChR antagonists could also inhibit the effects of acupuncture. 5. The turnover rate of ACh in diencephalon, caudate nucleus and spinal dorsal horn accelerated when acupuncture analgesia. The discussion was given on the variation of metabolic dynamics in combination with related literature.

Acetylcholine↗
PubMed1990

Effects of trans-Cinnamic Acid on Expression of the Bean Phenylalanine Ammonia-Lyase Gene Family.

Using DNA probes specific for the three members of the bean (Phaseolus vulgaris L.) phenylalanine ammonia-lyase (PAL) gene family, we have studied the effects of the product of the PAL reaction, trans-cinnamic acid (CA), on the appearance of individual PAL transcripts in suspension cultured bean cells. Concentrations of CA in excess of 10(-4) molar inhibited appearance of elicitor-induced transcripts encoding PAL1, PAL2, and PAL3 when added to the cells at the same time as fungal elicitor. Addition of CA 4 hours postelicitation caused a major reduction in the levels of all three PAL transcripts, but with different kinetics and subsequent rates of recovery. The inhibition of accumulation of PAL1, PAL2, or PAL3 transcripts, measured 3 hours after exposure to elicitor, as a function of the time of addition of CA postelicitation reflected the different rates of appearance of the three PAL transcripts in the presence of elicitor alone. The inhibitory effects of CA as seen on PAL transcripts were not observed for the constitutively expressed transcript H1, or the elicitor-inducible 1,3-beta-D-glucanase. Analysis of in vitro translated polypeptides showed that some elicitor-induced mRNA activities were not down-regulated by CA, and that a number of other mRNA activities were induced by CA, thus providing further evidence for specificity in the action of CA on bean cells. Treatment of elicited cells with L-alpha-aminooxy-beta-phenylpropionic acid, a potent and specific inhibitor of PAL activity, resulted in maintenance of elevated PAL transcript levels beyond 12 hours postelicitation, this effect being greatest for PAL transcripts 2 and 3. Our results are consistent with a model in which CA, or a derivative, could act as a component in a regulatory feedback system operating at the level of phenylpropanoid gene transcription.

Journal Article↗
PubMed1990

Variability in the functional activity of vaccine-induced antibody to Haemophilus influenzae type b.

Sera from 3 of 30 adults vaccinated with Haemophilus influenzae type b polysaccharide vaccine (Hib PS) had poor complement-mediated bacterial activity despite the presence of anti-Hib PS antibody concentrations of 8.6 to 20.5 micrograms/mL. These "nonkiller" antibodies killed less than 0.4 log cfu/mL compared to greater than 3 logs with all but one of the other sera. To investigate the basis of this poor functional activity, we characterized in detail the IgG antibodies to Hib PS present in two of the nonkiller sera, and compared the results with two of the "killer" sera. The latter were selected based on comparable levels of total antibody to Hib PS. No consistent differences were found between the relative proportions of IgG or IgA antibody to total anti-Hib PS antibody, or the respective ratios of IgG1 to IgG2 antibody in the nonkiller and killer sera. IgG fractions, and IgG affinity purified antibody to Hib PS were prepared. When tested at 2 micrograms/mL of antibody, the IgG fractions from the two nonkiller sera had much lower bactericidal activity than the corresponding fractions from the killer sera (3 logs less killing), and the former also had lower complement-mediated opsonic activity (20 and 13% uptake by human PMN compared to 62 and 93%). These data show the striking variability in the functional activity of vaccine-induced antibody to Hib PS. Antibody functional activity is likely to be affected by a number of factors but one important variable appears to be avidity since the IgG anti-Hib PS antibody from the two nonkiller sera had 2- to 5-fold lower avidity than the IgG antibody from the two killer sera.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1990

Detection of HPV-16 DNA in cervical carcinoma by paraffin section in situ hybridization.

Thirty-eight cervical carcinomas and 1 cervical condyloma were examined for the presence of human papillomavirus (HPV) type 16 DNA using 3H-dCTP-labelled HPV-16 DNA probe in paraffin section in situ hybridization and 32P-dCTP-labelled probe in dot blot hybridization (Tm-17). The results showed an HPV-16 DNA positive rate in our series of about 74.4% (29/39) as determined by paraffin section in situ hybridization and about 71.8% (28/29) by dot blot hybridization. There was no significant difference between the two methods. We thus confirmed that paraffin section in situ hybridization is an informative, reliable, and sensitive method for the diagnosis of cervical HPV infection.

Condylomata Acuminata↗
PubMed1990

[The effect of extradural injection of capsaicin on pain threshold and electroacupuncture analgesia of rat].

The present paper is designed to investigate the variation of pain threshold and electroacupuncture analgesia of rat, and to observe the immunohistochemical changes in spinal ganglion and dorsal horn of lumber and sacral segment of spinal cord after extradural administration of capsaicin (100 micrograms/rat). It was found: 1. Obvious decrease of pain threshold 60 mins after injection of capsaicin, and the pain threshold restored nearly to the level of pre-treatment at 90 mins of post-treatment of capsaicin. 2. Apparent elevation of pain threshold on 1st-15th day after injection of capsaicin. 3. The efficiency of electroacupuncture analgesia 7th day after injection of capsaicin was obviously weaker than that of pre-treatment of drug and that of solvents treatment in the animals of control group. 4. The SP-LI, ENK-LI and FRAP were all weakened in dorsal horn of spinal cord 1h after capsaicin treatment. However, on the 7th day, with the exception of decrease of SP-LI and FRAP, the ENK-LI in dorsal horn was strengthened and the activity of FRAP in spinal ganglionic cells of B type disappeared. The foregoing results indicated that the metabolism and function of C fibers of primary afferent especially the SP containing nerve and those of ENK-containing neurons could be influenced by the extradural injection of capsaicin. And the primary afferent C fiber (including the SP containing nerve) may play important roles in the input of the informations of pain, and needling, as well as in the acupuncture analgesia.

Acupuncture Analgesia↗
PubMed1990

[Acetylcholine and the primary input of acupuncture sensation--influence of peripheral acetylcholine on the role of electroacupuncture analgesia].

In order to investigate the correlation between the peripheral ACh and the primary input of acupuncture sensation, in the paper the cholinesterase inhibitor--Neostigmine and the ACh synthesis blocker--Hemicholine, which are unable to pass through blood brain barrier, and ACh were used as tools to increase or decrease the level of ACh in peripheral nerve system of rats. The results indicated that: 1) The effect of electroacupuncture analgesia can be enhanced by subcutaneous injection of neostigmine which related to the dosage used. 2) The influence of electroacupuncture analgesia can be markedly inhibited by intraperitoneal injection of Hemicholine. 3) This influence of suppression by Hemicholine can be reversed at once when acetylcholine in combination with neostigmine was injected. But could not reverse by neostigmine alone. It suggested that the effect of electroacupuncture analgesia and the primary input of acupuncture sensation were significantly related to the level and content of ACh in periphery.

Acetylcholine↗
PubMed1990

[Obliterative peripheral retinal vascular disease in children].

Obliterative peripheral retinal vascular disease in children is a rare disease. Only 4 cases had been reported abroad. No case of this disease was reported in China before. This paper reported a 13-year-old girl suffering from this disease in both eyes. Neovascular glaucoma of her left eye has developed and that of her right eye was still in early stage. Fluorescein angiography changes, diagnosis and differential diagnosis, pathogenesis and treatment of the disease were discussed.

Adolescent↗
PubMed1989

Environmental factors and Parkinson's disease: a case-control study in China.

We studied the role of environment in the development of Parkinson's disease (PD) in China, where industrialization is relatively recent and the population geographically stable. Using a case-control method, we investigated the relationship between PD and exposure to the following factors before disease onset: place of residence, source of drinking water, environmental and occupational exposure to various agricultural and industrial processes. Occupational or residential exposure to industrial chemicals, printing plants, or quarries was associated with an increased risk of developing PD. In contrast, living in villages and exposure to the common accompaniments of village life, wheat growing and pig raising, were associated with a decreased risk for PD. PD cases and controls did not differ with respect to other factors investigated. These findings are consistent with the hypothesis that environmental exposure to certain industrial chemicals may be related to the development of PD.

Adult↗