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Biomedical subjects

X L Yang

Publications and source records attributed to X L Yang.

At least 19 recordsLinked to original sources
PubMed2006

End-stage renal disease risk equations for Hong Kong Chinese patients with type 2 diabetes: Hong Kong Diabetes Registry.

AIMS/HYPOTHESIS: The objective of the study was to investigate risk factors and develop risk equations for end-stage renal disease (ESRD) in Chinese patients with type 2 diabetes. SUBJECTS AND METHODS: A prospective cohort of 4,438 patients with type 2 diabetes mellitus and without ESRD (median observation period 2.9 years, interquartile range 1.6-4.1 years) was included in the analysis. The end-point (ESRD) was defined by: (1) death due to diabetes with renal manifestations or renal failure; (2) hospitalisation due to renal failure; (3) estimated GFR (eGFR) <15 ml min(-1) 1.73 m(-2). Cox proportional hazards regression was used to develop risk equations. The data were randomly and evenly divided into the training data for development of the risk equations and the test data for validation. The validation was performed using the area under the receiver operating characteristic curve (aROC), which takes into account follow-up time and censoring. RESULTS: During the observation period, 159 patients or 12.45 per 1,000 person-years (95% CI 10.52-14.37 per 1,000 person-years) developed ESRD. Known duration of diabetes, systolic blood pressure, log(10) total cholesterol:HDL cholesterol ratio and retinopathy were significant predictors of ESRD. After further adjusting for eGFR, log(10) spot albumin:creatinine ratio (ACR) and haematocrit, only eGFR, haematocrit and log(10) ACR remained as independent predictors of ESRD. The risk equation derived from these three independent predictors had good discrimination, with an aROC of 0.97. CONCLUSIONS/INTERPRETATION: Estimated GFR, haematocrit and ACR were independent predictors of ESRD and the derived risk equation performed well in Chinese patients with type 2 diabetes.

Asian People↗
PubMed2006

Two-step phase-shifting interferometry and its application in image encryption.

Conventional phase-shifting interferometry (PSI) needs at least three interferograms. A novel algorithm of two-step PSI, with an arbitrary known phase step, by which a complex object field can be reconstructed with only two interferograms is proposed. This algorithm is then applied to an information security system based on double random-phase encoding in the Fresnel domain. The feasibility of this method and its robustness against occlusion and additional noise attacks are verified by computer simulations. This approach can considerably improve the efficiency of data transmission and is very suitable for Internet use.

Journal Article↗
PubMed2006

Preparation of bioelectret collagen and its influence on cell culture in vitro.

Collagen extracted from pigskin was corona-charged negatively by a specifically designed device. Different charging voltages, temperatures and times were applied to prepare collagen bioelectret. The decline of the surface potential of the bioelectret under different treatment was then determined. The data showed that the surface potential was markedly varied with the charging conditions. The optimal values of three parameters for charging collagen coatings were defined as follows: voltage, 8 KV; temperature, 40 degrees Celsius; time, 25 min. Treatment of the bioelectret with distillated water, or saline solution (0.9%) or culture medium induced a sharp decrease of the surface potential. In addition, we investigated the effects of the charged collagen on cell growth and intracellular calcium level of three types of cultured mammalian cell lines, including Chinese hamster ovary CHO cells, human cervix uteri tumor HeLa cells and human promyelocytic HL-60 cells. Cell growth and the intracellular calcium level were determined by MTT reagent-based assay and a fluorescent probe Fura-2, respectively. The results showed that negatively charged collagen stimulated the growth of CHO or HL-60 cell line but inhibited the growth of HeLa cell line. Furthermore, after attaching to the charged collagen, the intracellular calcium level of CHO cells increased, while that of HeLa cells decreased. Thus we proposed for the first time that collagen bioelectret could differentially modulate the growth of different cells, by an unknown mechanism that probably involves a role of intracellular calcium.

Animals↗
PubMed2005

Respiratory flow in obstructed airways.

Chronic obstructive pulmonary disease (COPD) is one of the most common diseases in human community. The COPD always results in inflammation that leads to narrowing and obstruction of the airways. The obstructive airways have significant effect on respiratory flow. In order to understand the flow phenomenon in such obstructive airways, four three-dimensional four-generation lung models based on the 23-generation model of Weibel [1963. Morphometry of the Human Lung. Springer, Academic Press, Berlin, New York] are generated. The fully three-dimensional incompressible laminar Navier-Stokes equations are solved using computational fluid dynamics (CFD) solver on unstructured tetrahedral meshes. Therein, a symmetric four-generation airway model is served as the reference, the other three models are considered to be obstructed at each generation, respectively. The calculation results show that the obstructive airway has significant influence on the air flow in both up- and down-stream airways and it even results in flow separation in the conjunction region. The re-circulation cell blocks the air from entering the downstream branches. This may be the reason why COPD patients should breathe gently, and this also provides some valuable information for medicine powder deposition.

Airway Resistance↗
PubMed2004

The effect of inlet velocity profile on the bifurcation COPD airway flow.

The effect of inlet velocity profile on the flow features in obstructed airways is investigated in this study. In reality, the inlet velocity distributions on such models, which are extracted from medial branches of natural human lung, should be neither uniform, nor symmetric parabolic, but skewed-parabolic due to having been skewed by the upper carina ridges. Four different three-dimensional three-generation models based on the 23 generations model of Weibel have been considered, respectively. The fully three-dimensional incompressible laminar Navier-Stokes equations and continuity equation have been solved using CFD solver on unstructured tetrahedral meshes. To reduce the complexity of the simulations, only one Reynolds number of 900 was used in this calculation. Four types of inlet boundary conditions, namely uniform, parabolic, positive-skewed parabolic (skewed to the positive x-direction), and negative-skewed parabolic, were imposed on the obstructed airway models, which were considered to be obstructed at either the second generation or the third generation airways, respectively. The results show that the inlet velocity profile has significant influence on the flow patterns, mass distributions, and pressure drops in either the symmetric model, or the three obstructed models. The three generation airways may not be enough to study the bifurcation flow in chronic obstructive pulmonary disease (COPD) airways, and a four-generation or more airway model is necessary to get better predictive results.

Computer Simulation↗
PubMed2004

Generalized phase-shifting interferometry with arbitrary unknown phase steps for diffraction objects.

A general method of extracting the arbitrary unknown and unequal phase steps in phase-shift interferometry from interferograms recorded on the diffraction field of an object and then reconstructing the object wave front digitally with our derived formulas is proposed. The phase steps are first calculated based on the statistical nature of the diffraction field and are further improved by an iterative approach. This method is simple, highly accurate, and usable for any frame number N (N > or = 3) and for both smooth and diffusing objects, as is verified by a series of computer simulations.

Journal Article↗
PubMed2003

Phase-shift extraction and wave-front reconstruction in phase-shifting interferometry with arbitrary phase steps.

A new approach to reconstructing the object wave front in phase-shifting interferometry with arbitrary unknown phase steps is proposed. With this method the actual phase steps are first determined from measured intensities with an algorithm based on the statistic property of the object phase distribution in the recording plane. Then the original object field is calculated digitally with a derived formula. This method is simple, accurate, and capable of retrieving the original object field, including its amplitude and phase distributions simultaneously, with arbitrary and unequal phase steps in a three- or four-frame method. The effectiveness and correctness of this approach are verified by a series of computer simulations for both smooth and diffusing surfaces.

Journal Article↗
PubMed2003

Interference of four umbrellalike beams by a diffractive beam splitter for fabrication of two-dimensional square and trigonal lattices.

A simple optical interference method for fabricating two-dimensional square and trigonal lattices is demonstrated. A general formula for the interference contrast formed by two arbitrary polarized elliptical waves is deduced, the relation between wave vectors of incident light and the resultant pattern is analyzed, and polarization optimization of all beams to ensure uniform contrast is given.

Journal Article↗
PubMed2003

Cholinergic and dopaminergic amacrine cells differentially express calcium channel subunits in the rat retina.

Immunofluorescence labeling was performed to study the expression of high voltage-activated Ca(2+) channel subunits on rat retinal cholinergic and dopaminergic amacrine cells, which were double labeled with antibodies against choline acetyltransferase and tyrosine hydroxylase, respectively. The alpha(1A) subunit was predominantly expressed on the processes but not on the somata of cholinergic amacrine cells, whereas staining for alpha(1B) and alpha(1E) was observed in both structures of the cells. Immunoreactivity of alpha(1C) and alpha(1D) was not found in the cholinergic amacrine cells. Dopaminergic amacrine cells, on the other hand, exhibited a differential expression pattern of the Ca(2+) channel subunits, with alpha(1A), alpha(1C) and alpha(1E) being expressed on both somata and processes and alpha(1B) predominantly on the processes of the cells. No alpha(1D) labeling was seen. These results suggest that Ca(2+) channel subunits differentially expressed on the cholinergic and dopaminergic amacrine cells may endow these two cell types with different physiological properties.

Acetylcholine↗
PubMed2002

Glycinergic synaptic transmission to bullfrog retinal bipolar cells is input-specific.

Glycinergic inhibitory postsynaptic currents (IPSCs) focally elicited at the dendrites and axon terminals were recorded from bipolar cells in the bullfrog retinal slice, using the whole-cell clamp technique. IPSCs driven by input from interplexiform cells at bipolar cell dendrites (ipc-IPSCs) had a much slower decay time constant (25.2 +/- 7.8 ms) than IPSCs driven by input from amacrine cells at bipolar cell axon terminals (ac-IPSCs) (14.7 +/- 5.5 ms). Furthermore, peak-scaled non-stationary noise analysis revealed that the weighted mean single-channel conductance of the glycine receptors underlying bipolar cell dendritic ipc-IPSCs (20.8 +/- 6.6 pS) was significantly larger than that of those underlying bipolar cell axon terminal ac-IPSCs (12.9 +/- 2.9 pS). These results demonstrate that glycinergic synaptic transmission with different properties at bipolar cell dendrites and axon terminals differentially mediates intraretinal centrofugal signal transfer from the inner retina to the outer retina provided by interplexiform cells and lateral inhibition offered by amacrine cells in the inner retina.

Animals↗
PubMed2002

Photo-induced cytotoxicity of malonic acid [C(60)]fullerene derivatives and its mechanism.

The biological activities of fullerenes have attracted extensive attention in recent years. The aim of this paper is to study the relation of the photo-induced cytotoxicity of fullerene derivatives to their chemical structures as well as the possible cellular mechanism involved in the photocytotoxicity. Three C(60) derivatives with two to four malonic acid groups (DMA C(60), TMA C(60) and QMA C(60)) were prepared and the cytotoxicity of these compounds against HeLa cells was determined by MTT. Cell cycle was measured by flow cytometry. The results showed that the cytotoxicity of the malonic acid C(60) derivatives was irradiation- and dose-dependent. The sequence of their photo-induced growth inhibition was DMA C(60)>TMA C(60)>QMA C(60). Hydroxyl radical quencher mannitol (10mM) was not able to prevent cells from the damage induced by irradiated DMA C(60). DMA C(60), together with irradiation, was found to have an ability of inducing a decrease in the number of G(1) cells from 63 to 42% and a rise in that of G(2)+M cells from 6 to 26%. These data indicated that the number of malonic acid molecules added to C(60) played an important role in the phototoxicity, and the blockage of cell cycle might be a mechanism of this activity.

Carbon↗
PubMed2002

Formation of three-dimensional periodic microstructures by interference of four noncoplanar beams.

A newly reported method of making three-dimensional microstructures or photonic crystals by holographic lithography has some obvious advantages over other techniques with the same purpose. A systematic and comprehensive analysis of interference of four noncoplanar beams (IFNB) is provided. It shows that all 14 Bravais lattices can be formed by means of IFNB and gives explicit relationships between each lattice and the corresponding recording geometry. The concept of pattern contrast is extended to the case of IFNB, and it is indicated that a uniform contrast for each interference term can be obtained by properly choosing the beam ratio and polarization. A calculation algorithm is then developed to optimize the direction of polarization of each beam to ensure maximum uniform contrast. These results, verified by computer simulations, may lay a theoretical foundation for fabrication of photonic crystals with the approach of IFNB.

Journal Article↗
PubMed2001

Glutamate transporter EAAC1 is expressed on Müller cells of lower vertebrate retinas.

The expression of glutamate transporter EAAC1 was investigated in carp and bullfrog retinas using Western blotting, immunofluorescence double labeling and confocal laser scanning microscopic techniques. In addition to a variety of retinal neurons, radially oriented elements spanning the whole neural retinas of carp and bullfrog were also EAAC1-immunoreactive, and EAAC1 was found to be predominantly on the cell membrane. Virtually all EAAC1-labeled radial elements were immunopositive to glial fibrillary acidic protein (GFAP), a specific marker for retinal Müller cells of carp and bullfrog, indicating that they were Müller cells. This finding suggests that EAAC1, which has been thought to be an exclusively neuronal type, may be a glial transporter as well. EAAC1 of Müller cells may play an important modulatory role in the retina by making contributions to glutamate homeostasis.

Amino Acid Transport System X-AG↗
PubMed2001

Release of hydrogen chloride from combustibles in municipal solid waste.

Study of the inorganic chlorides in municipal solid waste (MSW) shows that the main source of inorganic chlorides in MSW is food. The main organic source of HCl emission from MSW is plastic. But wood, textiles, and food also produce a large amount of HCl when they are combusted. Each combustible shows a different HCl releasing temperature range. At 973 K, there are 30-70% of the total chlorine left in the char of each combustibles in MSW.

Air Pollutants↗
PubMed2001

Zn2+ differentially modulates signals from red- and short wavelength-sensitive cones to horizontal cells in carp retina.

The effects of Zn2+ were studied while recording intracellularly from L-type horizontal cells (LHCs) in the isolated, superfused carp retina. In darkness, 25 microM Zn2+ hyperpolarized LHCs and potentiated responses of these cells to 500 nm flashes, but decreased those to 680 nm flashes. Zn2+ did not change photopic electroretinographic P III responses. The differential modulation by Zn2+ persisted when the Zn2+-induced membrane hyperpolarization was compensated by lowering Ca2+ concentration in the perfusate, but it was abolished in the presence of background illumination. Furthermore, the differential modulation no longer existed in the presence of bicuculline, suggesting the involvement of gamma-aminobutyric acid(A) (GABA(A)) receptors. We speculate that the differential modulation may be a consequence of multiple changes caused by Zn2+. Decreased glutamate release from the cone terminal by Zn2+ results in a reduction of cone signals. Zn2+ antagonizes GABA receptors on LHCs, leading to cone signal reduction. On the other hand, Zn2+ may reduce the strength of the negative feedback from LHCs to cones by downregulating the activity of GABA receptors on the cone terminal, which causes a potentiation of LHC light responses. Cone- or wavelength-relevance of the Zn2+-induced feedback strength change may account for the differential modulation.

Animals↗