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X Jin

Publications and source records attributed to X Jin.

11 recordsLinked to original sources
PubMed1992

[Solid-phase extraction and RP-HPLC screening procedure for diuretics, probenecid, caffeine and pemoline in urine].

A solid-phase extraction and reversed phase high performance liquid chromatographic method (RP-HPLC) was developed for the rapid determination of 13 diuretics (belonging to five different pharmacological groups), probenecid, caffeine and pemoline in urine. Two ml urine sample was first adsorbed on a XAD-2 column, then eluted with ether-ethyl acetate (1:1). The eluate was evaporated to dryness and reconstituted in methanol. The methanolic solution was injected into a HP LiChrosorb RP-18 column, using phosphate buffer (pH 3) and acetonitrile as the mobile phase and monitored at 216 nm, 230 nm, and 275 nm on a diode array ultraviolet detector. The extraction recoveries of 16 drugs were above 75%. The limits of detection ranged from 0.3-3.0 micrograms/ml of urine. All drugs were separately administered to healthy volunteers, positive urine samples were collected, and urinary excretion-time curves of some drugs were reported.

Adult↗
PubMed1991

[Thrombosis test in vitro of 107 cases with arteriosclerosis obliterans].

Thrombosis test in vitro of 107 cases with arteriosclerosis obliterans were measured and controlled by 36 normal persons. Of 107 cases, 13 cases belonged to ischemic phase, 47 cases nutritional disturbance phase, 47 necrosis (24 cases were first degree, 9 cases second degree, 14 third degree). Types distribution were: simple blood stasis 65 cases, blood and damp stasis 34, and 8 others. 46 patients were treated systematically by TCM-WM. The result proved that the indexes of thrombosis test in vitro of patients were much higher than that of normal persons (P less than 0.001). The relevant indexes were different in varied periods, degrees and types (P less than 0.05-0.01) and between before and after treatment (P less than 0.001). Of treated 46 cases, total effective rate was 86.96%, amputation rate was 13.04%. Formation of thrombus in vitro lowered 88.03%. The authors suggested that measurement of thrombosis test might be a accurate way to reflect the course of the disease and a basis for treatment and differentiation of symptoms.

Adult↗
PubMed1990

[Clinical analysis of 200 cases of necrotic thromboangiitis obliterans].

UNLABELLED: Two hundred cases of necrosis thromboangiitis obliterans (199 males, 1 females; age 17 approximately 63 years) were observed. The illness course averaged more than 5 years. Focuses of upper limbs were 6 cases and those of lower limbs 194 cases. 98 cases and 102 cases suffered from ulcer and gangrene respectively. 100 cases belonged to the first degree, 62 cases to the second degree, 38 cases to the third degree of III stage. 171 cases were treated by the combination of TCM and WM except 3 cases which were actively discharged and 26 cases which were to be given amputation. RESULTS: 90 cases within 171 cases were cured, 52 cases were improved obviously, 7 cases improved, 5 cases ineffective and 17 cases to be given amputation. The writers regard three principles should be followed in the treatment of necrosis thromboangiitis obliterans by the combination of TCM and WM. (1) The main importance was to improve blood circulation to remove blood stasis. (2) Controlling infection was the key point, effective antibiotics and hormone should be added besides these herbal medicines for clearing away heat and toxic materials. (3) General protecting therapy, performing various local operations on right period on the basis of improvement of blood circulation of the involved limbs, the wound could heal successfully. The writers suggest that the amputation should not be given above the level of the main artery obliterans.

Adolescent↗
PubMed1990

[A mathematical model of age distribution of malignant tumours mortality].

There was a tendency that the mortality of malignant tumours increased with age. In order to explore the law of this tendency, the data of malignant tumours mortality from disease monitoring points in Shandong Province were mathematically analogized, and the mathematical model of age distribution of malignant tumours mortality was established by using the exponential curve, y = 10a + bx. The model was also tested and verified by using national data, to observe the universal significance of the model. The model gave a theoretical account of the law of age distribution of malignant tumours mortality from a population, and provided an initial method to predict malignant tumours mortality. The increment quantity of malignant tumours mortality in various age groups when age increased one year could be calculated by using the differential equation from the exponential curve equation, y = 10a + bx. Further, "an increment multiple constant" of the increment quantity of malignant tumours mortality could be calculated. The constant could be used as an Index for comparison in risk degree and age distribution law of malignant tumours mortality among various age groups and the same age group in different periods, and provided leads for further research of the causes of malignant tumours.

Age Factors↗
PubMed1990

[Formation of extrinsic blood thrombosis in 102 cases of thromboangiitis obliterans].

The formation of extrinsic blood thrombosis in 102 cases of thromboangiitis obliterans and 54 healthy persons were observed with chandler method and their clinical manifestations. All the 102 patients were male, of which 13 cases were in ischemic stage, 45 cases in dystrophic stage, and 44 cases in necrotic stage. According to the differentiation of TCM, 8 belong to Yin-cold type, 50 belong to downward flowing of damp-heat type, 42 belong to blood stasis type, and 2 others. Combining method of TCM-WM was adopted to give 45 cases systemic treatment, that is, taking the promotion of blood flow to remove its stasis as the dominant factor, accompanied by intravenous drip and operation. Results showed that the length, wet weight and dry weight of extrinsic blood thrombosis were higher apparently in the cases of thromboangiitis obliterans than those in healthy persons (P less than 0.001), and there were significant differences between the values of extrinsic blood thrombosis in different stages and different degrees. After systemic treatment with TCM-WM, the values of extrinsic blood thrombosis decreased remarkably, and their symptoms and signs improved or disappeared. The clinical total effective rate was 88.89%, and the decreasing rate of the values of extrinsic blood thrombosis was 98.9%. This suggested that the high blood coagulation and extrinsic thrombosis should draw special attention and may act as an approach reflecting the severity of the disease and evaluating the effects of treatment.

Adolescent↗
PubMed1988

Significance of seizures in very low-birthweight infants.

Of 506 very low-birthweight (VLBW) infants, the authors review data on 67 identified as having seizures. 29 survivors with seizures and 305 without were followed up at the ages of one, two and five years: statistical significance of psychological and mental impairment was evaluated. Impairment was higher in the seizure group at 66 per cent (15 per cent in non-seizure group) and 43 per cent of these were considered to be severely impaired. Infants with seizures occurring for more than seven days and lasting for more than five minutes had the poorest outcome, and infants with late onset had the best outcome. Analysis showed that 40 per cent of seizures were caused by birth asphyxia, and these were strongly prognostic, correlating with mortality and impairment rates. 60 per cent of the infants who died had the same cause for both their seizures and death, and for 57 per cent of these the cause was perinatal asphyxia. The poor outlook for the VLBW infants suggests that the seizures themselves are a serious neurological insult.

Female↗
PubMedDate not supplied

Signal transduction in activated natural killer cells and natural killer cells inactivated with sensitive targets.

We have recently demonstrated that the activation of NK cells involves a protein-kinase-C (PKC)-dependent step as well as a phosphatidylinositol (PI)-linked signal transduction system. We have also shown that when NK cells are incubated with sensitive targets for up to 6 h they lose their lytic potential and require IL-2 to regain their lytic activity. PKC is involved in many cell processes such as the transduction of hormonal signals and the machinery of cellular secretion and is activated by diacylglycerol and by a number of phorbol esters, including phorbol myristic acid (PMA). To reinforce the role of PKC in NK-cell-mediated cytotoxicity, we first showed that NK-CMC is inhibited by two agents that inhibit PKC activation, staurosporine and sphingosine. Next, we showed that antibody-dependent cellular cytotoxicity of NK-resistant targets was PKC dependent and Ca2+ dependent. Further, we showed that PMA plus ionophore alone could induce NK killing of resistant targets and that this killing was PKC and Ca2+ dependent. Finally, we contrasted the role of PKC in these activated cells to the role of PKC in cells that have been inactivated with a sensitive target, K562. We showed that PKC is not required for the IL-2-dependent reactivation of NK cells but that Ca2+ is required. To further determine what event in signal transduction is inactivated by K562, we showed that inactivated NK cells do not turnover PI in response to K562 stimulation.

1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine↗