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Biomedical subjects

X Jiao

Publications and source records attributed to X Jiao.

At least 37 records · Page 2Linked to original sources
PubMed1998

[The estrogenic activities of 2-[3-estrone-N-ethyl-piperazine-methyl]tetracycline(XW630)--a new compound with anti-osteoporosis activity].

2-[3-Estrone-N-ethyl-piperazine-methyl] tetracycline (XW630) showing anti-osteoporosis activity is a new compound with piperazine-tetracycline in the steroid structure of estrone. The affinity of XW630 with uterine estrogen receptor and its estrogenic activity were studied in this paper. The relative affinities of estrone, estradiol and XW630 with estrogen receptor (ER) of the uterine myoma tissues were found to be 0.325, 1.000 and 0.011, respectively, by the competitive assay. The estrogenic activity index of estrone, estradiol and XW630 were shown to be 9.70, 10.30 and 6.90, respectively, by the uterine weight assay using immature mice. These indexes indicate that: the estrogenic activity of XW630 is about 1/1960 that of estrone and 1/7742 that of estradiol. The vaginal smear assay of oophorectomized mice also showed that the estrogenic activity of XW630 is less than that of estrone and estradiol.

Animals↗
PubMed1997

The value of cervical mediastinoscopy combined with anterior mediastinotomy in the peroperative evaluation of bronchogenic carcinoma of the left upper lobe.

METHODS: From January 1990 to July 1994, 85 patients who were otherwise thought to have an operable tumour within the left upper lobe underwent left anterior mediastinotomy supplemented by cervical mediastinoscopy in 75 cases. This combined approach allowed assessment of nodal involvement within the superior and anterior mediastinal areas, the detection of direct tumour invasion into the mediastinum and the determination of resectability by bidigital examination of the area around the aortic arch and sub-aortic fossa. RESULTS: It was found that 27 (31.8%) patients were inoperable, either because of nodal involvement at cervical mediastinoscopy (4 patients) or because of extension into the mediastinum at left anterior mediastinotomy (14 patients), or because of positive results from both methods (9 patients). The inoperability determined by this examination for patients with adenocarcinoma (8/18, 44.4%) is higher than for patients with squamous carcinoma (12/52, 23.1%). All of the 58 patients with negative findings proceeded to thoracotomy and complete resection was possible in 54 patients (93.1%). CONCLUSION: We conclude that this combined approach is better than using either technique alone in the preoperative staging and the evaluation of resectability of left upper lobe tumours.

Adenocarcinoma↗
PubMed1997

Effect of sedative and hypnotic doses of propofol on the EEG activity of patients with or without a history of seizure disorders.

Propofol is alleged to possess both pro- and anticonvulsant properties, leading to controversy regarding its use in patients with a history of seizures. Since propofol is administered for both sedation and hypnosis, it is important to understand the effects of low (0.5-1.0 mg/kg) and high (2-2.5 mg/kg) doses of propofol on the electroencephalogram (EEG). In this study, the hemodynamic and EEG effects of cumulative doses of propofol from 0.5 to 2.5 mg/kg i.v. were studied in 30 neurosurgical patients with or without a history of seizure disorders. While continuously recording from scalp EEG electrodes (F3, F4, C3, C4, P3, P4, O1, and O2), propofol 0.5 mg/kg was infused intravenously over 20 s. The same dose of propofol was reinjected four times at 2-min intervals, until a total dose of 2.5 mg/kg had been administered. The number and average amplitude of the EEG waves were counted and measured manually, respectively, from 80 to 90 s after beginning the injection of each dose of propofol. After lower propofol doses (0.5-1 mg/kg), the number of beta-waves increased, while alpha- and theta-waves decreased significantly in all patients. However, with larger doses of propofol (total dose of 2-2.5 mg/kg), the number of beta-waves decreased and delta-waves appeared. The amplitudes of all EEG waves increased and were maintained at a higher level after administration of propofol. Spike (or sharp) waves appeared in 33% of the control patients and in 40% of the epileptic group after propofol 0.5 mg/kg and in 73% of the control and 67% of the epileptic patients after the 1.5-mg/kg dose. In the majority of patients, the spike waves disappeared when additional doses of propofol were administered. One patient in the epileptic group had an EEG-recorded and clinical grand mal seizure after propofol 1 mg/kg, but the seizure disappeared after an additional 0.5-mg/kg bolus dose was administered. The propofol-induced EEG changes appeared initially at the frontal and central EEG electrodes and subsequently at the other EEG electrodes. Overall, there were no significant differences in the spectrum of EEG changes between the two patient populations. It is concluded that propofol produces similar dose-dependent effects on EEG activity in patients with or without a history of seizure disorders. While induction of anesthesia with higher doses of propofol (> 1.5 mg/kg) in neurosurgical patients with well controlled seizure disorder is safe, smaller sedative doses should be administered with caution to epileptic patients.

Adult↗
PubMed1997

[Study of the relationship between human quality and reliability].

To clarify the relationship between human quality and reliability, 1925 experiments in 20 subjects were carried out to study the relationship between disposition character, digital memory, graphic memory, multi-reaction time and education level and simulated aircraft operation. Meanwhile, effects of task difficulty and enviromental factor on human reliability were also studied. The results showed that human quality can be predicted and evaluated through experimental methods. The better the human quality, the higher the human reliability.

Aviation↗
PubMed1997

Phase II trial in China of a new, rapidly-acting and effective oral antimalarial, CGP 56697, for the treatment of Plasmodium falciparum malaria.

One hundred and two Chinese out-patients with naturally acquired, previously untreated, falciparum malaria were selected to evaluate the efficacy of a new combination anti-malaria therapy, CGP 56697 (artemether plus benflumetol). In this open non-comparative trial each patient received a combination of 80 mg artemether and 480 mg benflumetol given orally at 0, 8, 24 and 48 hours (total: 320 mg artemether, 1,920 mg benflumetol). Patients were kept for 28 days in a transmission-free hospital in an area with chloroquine resistant falciparum malaria to prevent reinfection and to aid diagnosis of recrudescence. Progress and possible adverse effects were monitored by blood film parasitology, blood biochemistry assays, urinalysis, ECG and X-ray. Ninety-eight of the 102 patients were shown to be free of infection at 28 days, a 96.1% cure rate. Parasite reduction at 24 hours was 99.4%. Time to effect complete parasite clearance ranged from 24 to 54 hours (median 30 hours). Time for fever clearance ranged from 6 to 78 hours (median 18 hours). Recrudescence was low (3.9%). No significant adverse side-effects were encountered. It is concluded that CGP 56697, a combination anti-malaria therapy of artemether with benflumetol, offered a rapid and highly effective treatment for acute uncomplicated falciparum malaria in an area of chloroquine-resistant malaria in China.

Administration, Oral↗
PubMed1997

[A study on neurotoxicity of acrylamide and glycidamide].

Glycidamide (GA) is an epoxide metabolite of acrylamide (AA). Neurotoxicity of both GA and AA was compared by their performance in behavioral, neuro-biochemical and neuropathological studies. Male rats were injected peritoneally with AA (25 or 50 mg/kg) or GA (50 or 100 mg/kg) once daily for eight days. Results showed that toxic effects of AA were greater than those of GA in wheel rotation test, hind limb splay test, and activities of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in peripheral nerves in rats. Results of neuropathological examinations in experimental rats also provided evidence that AA could cause damage to peripheral nerves, but GA did not. It indicated that nerve tissue was more vulnerable to AA than to GA. Also, it suggested that neurotoxic effects of AA was caused by itself, and influenced less by its metabolite GA.

Acrylamides↗
PubMed1996

Noninvasive measurement of protein concentration.

At selected magnetic field strengths, protein and water proton spin-lattice relaxation rates are sensitive to the concentration of rotationally immobilized peptide nitrogen because of field dependent heteronuclear cross relaxation coupling between protein proton and nitrogen-14 spins that is carried to the water by proton homonuclear cross-relaxation. Measurement of the water proton spin-lattice relaxation time, or a signal amplitude proportional to it, may provide a noninvasive measure of peptide bond concentration, which provides a direct measure of immobilized protein content in most tissues. The approach using protein gels in two magnetic field strengths is demonstrated. At 66.7 mT; the proton Zeeman energy matches one of the peptide nitrogen transitions dominated by the unaveraged nuclear electric quadrupole interaction; cross-relaxation between the protons and nitrogen-14 is efficient. At 77.5 mT, the proton Zeeman energy is not matched with the nitrogen energy and proton-nitrogen cross-relaxation is not efficient. It is shown that the difference in the water proton spin-lattice relaxation rates on and off the energy level match condition is a linear function of the rotationally immobilized protein concentration.

Animals↗
PubMed1996

Effect of huoxuequyu recipe on erythrocyte ultrastructure and membrane ATPase activity in rats with passive smoking.

The effect of "Huoxuequyu" recipe on erythrocyte membrane Ca(2+)-ATPase activity, Na(+)-K(+)-ATPase activity and erythrocyte ultrastructure in rats exposed to passive smoking were observed. The erythrocyte membrane Ca(2+)-ATPase activity and Na(+)-K(+)-ATPase activity were found to be decreased in the model group as compared with the control group (P < 0.01, P < 0.01), while no significant difference existed between the treated group and the control group (P > 0.05). Our results also showed that there were more deformed red blood cells in model group than in control group under TCM and SEM (P < 0.0001). No significant difference was found between control group and treated group (P > 0.05). Furthermore, these findings indicated that "Huoxuequyu" recipe, a prepared natural herbal medicine, may be involved in modulation of the activity of erythrocyte membrane enzymes, thereby protecting erythrocyte membrane, so as to improve the erythrocyte function.

Adult↗
PubMed1996

Pericardial repair after extensive resection: another use for the pedicled diaphragmatic flap.

BACKGROUND: Extended resection for pulmonary malignancy frequently leaves a large pericardial defect, sometimes associated with resection of the phrenic nerve. On the left the defect does not require repair; as long as the defect is sufficiently large to avoid constriction, the heart can herniate freely. On the right such herniation is associated with venous inflow occlusion and death. The pedicled diaphragmatic flap has been used in other situations in thoracic surgery. We have modified this to allow closure of the pericardial defect and concurrent plication of the denervated diaphragm. It may also be used to cover the bronchial stump or a bronchial anastomosis. METHODS: The flap has been used in 13 patients over an 11-year period. RESULTS: Secure closure of the pericardial defect has been achieved in all patients with satisfactory plication of the diaphragm. Reoperation for bleeding was necessary in 3 patients, but in only 1 was the diaphragm shown to be the site of bleeding. Patients otherwise made an uneventful recovery. CONCLUSIONS: A large pedicled flap of redundant diaphragm provides secure closure for large pericardial defects after extended right pneumonectomy.

Adult↗
PubMed1996

Interaction of electron leak and proton leak in respiratory chain of mitochondria--proton leak induced by superoxide from an electron leak pathway of univalent reduction of oxygen.

By incubating the isolated rat myocardial mitochondria with xanthine-xanthine oxidase, an exogenous superoxide (O2.-) generating system, and by ischemia-reperfusion procedure of isolated rat heart as an endogenous O2.- generating system, it was found that both sources O2.- showed the same injurious effects on mitochondrial function resulting in (i) increasing proton leak rate, lowering proton pumping activity and H+/2e ratio of respiratory chain, and (ii) decreasing transmembrane potential of energized mitochondrial inner membrane by succinate oxidation. The injurious effects of O2.- on these mitochondrial bioenergitical parameters mentioned above exhibited a dosage- or reaction time-dependent mode. O2.- has no effects on the electron transfer activity and transmembrane potential of nonenergized mitochondria. Being a superoxide scavenger, 3,4-dihydroxylphenyl lactate showed obvious protection effects against damage of both exogenous superoxide sources from xanthine-xanthine oxidase system and endogenous O2.- source from ischemia-reperfused heart on proton pumping activity, H+/2e ratio and transmembrane potential of energized mitochondria. The experimental results presented here clearly showed that O2.- radicals induce an increase of proton leak in mitochondria directly related to the molecular mechanism of early phase damage of ischemia-reperfused heart, and also provide evidence to support our recent proposed hypothesis that electron leakage to oxygen to form O2.- in respiratory chain is one of the mechanical cause of proton leakage in mitochondria.

Animals↗
PubMed1996

[Cloning and identification of fliCi gene of Salmonella typhimurium].

Purified Salmonella typhimurium 8705 chromosomal DNA was digested with EcoRI. DNA fragments above 400 bp were obtained by Sepharcyl S-400 chromatography and cloned into vector pGEM-3Zf(-), then transformed into host cell LC2a (hag- is recA-). One out of 6013 transformants was found to be motile and this clone was named pGI4015. Miniprep proved that pGI4015 contained an inserted fragment about 15.3 kb. Motility/inhibition tests as well as Southern blot hybridization showed that pGI4015 bear the H-1i gene of S. typhimurium. With the aid of BamHI and SalI site, sequences unrelated to flagellin in the cloned DNA fragment was removed, and a 3.8kb fragment containing fliC gene was subcloned.

Cloning, Molecular↗
PubMed1995

The effect of mannitol and anisodamin on the prevention of free radical injury to post-ischaemia flaps: an experimental study.

Rat abdominal skin flaps were subjected to total venous occlusion for 8 h. Five minutes before release of the vascular occlusion, mannitol, mannitol plus anisodamin, anisodamin or placebo (0.9% normal saline) was administered intravenously. Drug treated flaps showed a statistically significant increase in the proportion of area surviving (P < 0.001). The combination of mannitol and anisodamin was not more effective than either agent alone in increasing the proportion of area surviving. The results of biochemical analyses indicated that neither mannitol nor anisodamin affected xanthine oxidase activity (p > 0.05) but that both agents significantly reduced the increase of malondialdehyde (MDA) concentration caused by ischaemia-reperfusion (p < 0.01). Treatment with mannitol or anisodamin also prevented the increase of lactate and water content and the decrease in glucose content in the island skin flap tissue which occurred on reperfusion. The data indicate that mannitol and/or anisodamin have the potential to salvage anticipated flap necrosis. It is possible that the mechanism of action is inhibition of damage caused by toxic oxygen species and improvement in capillary reperfusion.

Animals↗
PubMed1995

Soluble-binding proteins for docosahexaenoic acid are present in neural retina.

PURPOSE: To determine if soluble binding proteins (BP) for fatty acids (FA), particularly docosahexaenoic acid (DHA), could be identified in the cytosol of rat and bovine retinas under in vitro and in vivo conditions. METHODS: In vitro, cytosol fractions from normal bovine and rat retinas were delipidated and incubated with [14C]-DHA with or without a number of competing fatty acids. After crosslinking bound FA to BP, the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and radioactivity determined in each fraction. For in vivo experiments, rats received [14C]-DHA by gavage. At selected periods after ingestion, retinas were collected and cytosolic fractions were prepared from each. These were crosslinked and subjected to SDS-PAGE, and radioactivity was determined in each fraction. RESULTS: In vitro, several peaks of radioactivity (approximately 13, 20, 32, 43-45, 50, 63, and 94-105 kd) were found that exhibited [14C]-DHA binding. Relative specificity of binding was assessed by blocking of the [14C]-DHA binding with unlabeled DHA and by competition experiments with other FAs. After in vivo ingestion of [14C]-DHA, a large peak of radioactivity was observed at 43 kd by 4 hours. At 6 hours, this peak decreased and, after 24 hours, it approached baseline. CONCLUSIONS: These findings demonstrate that there is a discrete grouping of proteins in retinal cytosol capable of binding DHA and other FA. Although the identities of these proteins have yet to be determined, the group may include a member of the 12- to 15-kd group of small fatty acid binding proteins (FABP). In particular, a unique 43-kd binding peak could play a major role in the uptake, binding, or both of DHA by the retina in vivo.

Animals↗
PubMed1994

[Anastomosis of small artery using ZT medical adhesive and soluble stent].

The conventional technique of interrupted suture in microvascular anastomosis is difficult to perform and timeconsuming. A new method with ZT medical adhesive and soluble intravascular stent is introduced. Using this method, end-to-end anastomosis of small arteries could be performed easily and quickly. Both the patency rate and histopathologic changes at the anastomosis sites were examined. In comparison with suture technique, similar patency rate was obtained. Inflammatory reaction in mid and outer layers of the vessel was observable and reendothelialization of the intima was identical.

Anastomosis, Surgical↗
PubMed1991

[Applied anatomy of lymph drainage of lower lip mucosa].

The lymph drainage of lower lip mucosa was studied in 30 fresh cadavers of fetus and neonatus by submucosa injection of 30% Prussian blue of chloroform solution. The collecting lymphatics in lateral third of both the right and left sides of the lower lip mucosa ran laterally to the angles of the mouth, and then back- and downward between mandible and depressor muscle of angle of mouth, and finally, accompanying the facial vessels, emptied into submandibular lymph nodes. The efferent lymphatics of the latter emptied into angular lymph nodes and jugulo-omohyoid lymph nodes. The collecting lymphatics in the middle third of the lower lip mucosa went superficially to the mental muscle and downward into submental lymph nodes. The efferent lymphatic of the latter emptied, anteriorly to the body of the hyoid bone, latter- and downward into right and left jugulo-omohyoid lymph nodes. All collecting lymphatics lie in the loose connective tissue between the muscles.

Humans↗