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Biomedical subjects

X J Wu

Publications and source records attributed to X J Wu.

28 records · Page 2Linked to original sources
PubMed1990

[Surgical treatment of chronic constrictive pericarditis].

From April, 1969 to December, 1988, partial pericardiectomy was performed in 78 patients with chronic constrictive pericarditis. The long-term follow-up study in 60 patients showed a satisfactory functional result. The authors considers that the median sternotomy is a better approach, the effectiveness of operation should be gauzed by dropping the vena cave pressure below 140 mmH2O, resection of pericardium from left to right is logical, and rational use of cardiotonic agents is important.

Adolescent↗
PubMed1990

[Development of a new system for selection of A. foetidus transformed with foreign genes by using thymidine kinase gene as a marker and expression of HBsAg gene in A. foetidus].

A new system for selection of transformed Aspergillus foetidus was reported. In this system, TK- A. foetidus which were constructed by homologous recombination of mutated TK gene of vaccinia virus with TK gene of A. foetidus were screened by adding BUdR in agar plates. Conditions for screen of TK+ A. foetidus strain, transformation of A. foetidus and selection of transformed TK- A. foetidus have been studied. By using this system, several transformed A. foetidus which contained HBsAg gene derived bf a promoter H8 cloned from genomic DNA of A. foetidus were isolated. It was demonstrated that HBsAg gene was integrated into the chromosome DNA of A. foetidus by Southern blot after many passages of spores. ELISA showed that HBsAg was positive in the growth medium (p/n = 20). The 22 nm particles which were very similar to the HBsAg particles in human serum were found in the growth medium by immunoelectromicroscope. Western blot also gave the specific bands. All these data showed that HBsAg gene was expressed in A. foetidus and the products were secreted into the growth medium. The selection system using TK gene as marker could generally be used to study the expression of foreign gene in A. foetidus.

Aspergillus↗
PubMed1990

[An experimental study of the basic properties of drug microspheres and target treatment of rats with liver tumor].

This experimental hepatic artery embolization with Camptothecin (CPT) albumin microspheres was performed in normal rats and rats with liver tumor. The basic properties of the CPT albumin microspheres and the effects of target treatment on rats with liver tumor were studied. The results showed that the concentration of the drug released into hepatic vein was almost stable for over 3 hours after hepatic arterial infusion of CPT albumin microspheres, the partial and complete recovery of hepatic artery inflow occurred within 50-70 days after embolization. Pathological examination revealed many irreversible damages of the liver, and the tumor vessels and stains disappeared after hepatic arterial embolization, some of the tumor were decreased in size and had complete necrosis. Therefore, CPT albumin microspheres are peripheral biodegradable embolizatic substances with the property of slowly drug-releasing. It can exert the effect of chemoembolization in treating the hepatic malignant tumor, alleviating the toxic reaction of CPT and increasing the patient's tolerance to CPT.

Animals↗
PubMed1989

[Use of a narrow pedicled cross-finger flap to repair defects of the fingertip].

A narrow pedicled cross-finger flap procedure for repair of fingertip defect is described. Its shape resembles that of the so-called flag flap. This technique was applied to 15 cases (17 fingers) and satisfactory results were obtained. The advantages include easy to rotate, comfortable positioning and avoidance of joint contracture.

Adolescent↗
PubMed1989

[The effect of drug-microsphere embolization of hepatic artery on normal liver and experimental hepatic tumor in rats].

Experimental hepatic artery embolization with camptothecin (CPT) albumin microspheres was performed in normal livers of 18 rats and experimental hepatic tumors of 8 rats. The effects of drug microsphere embolization were evaluated by selective celiac angiography and pathologic examination. The results showed that hepatic artery and peripheral vessels totally disappeared on angiogram after intra-hepatic-arterial infusion of CPT microspheres. Plentiful collaterals were demonstrable on follow up selective celiac angiogram 20 days postoperative, while partial and complete reconstitution of hepatic artery occurred within 50 approximately 70 days after embolization. Pathologic examination revealed that the drug microspheres were entrapped in the arterioles of liver and scattered areas of infarction were observed in the peripheral parts of liver. Therefore, CPT albumin microsphere is a peripheral type of biodegradable embolizing agent.

Animals↗
PubMed1989

Effects of ginseng root saponins on brain monoamines and serum corticosterone in heat-stressed mice.

The rectal temperature and serum corticosterone increased in mice exposed to 45 degrees C for 15 min; at the same time, the contents of brain 5-HT and NE reduced, brain DA unchanged. Ginseng root saponins (GRS) ip 200 mg/kg inhibited the increase of serum corticosterone and the decrease of brain 5-HT and NE in heat-stressed mice, but did not affect brain DA. GRS lowered mice body temperature at room temperature and inhibited the rise of body temperature under heat environmental conditions in mice. Reserpine eliminated the hypothermia of GRS at room temperature and its inhibitory effect on hyperthermia under heat-stress conditions. PCPA eliminated only the inhibition of GRS on hyperthermia under heat-stress, but had no significant effect on hypothermia at room temperature.

Animals↗