Biomedical subjects

X Huang

Publications and source records attributed to X Huang.

At least 163 records · Page 9Linked to original sources

PubMed2001

MR and cine-MR imaging in the diagnosis of infantile congenital heart disease.

OBJECTIVE: To assess the effectiveness of magnetic resonance imaging (MRI) and cine magnetic resonance imaging (cine-MRI) in the diagnosis of infantile congenital heart disease. METHODS: A total of 34 cases were studied with MRI and cine-MRI. The data were analyzed and compared with those of two-dimensional echocardiography (2DE), cardioangiography (CAG) and surgery. RESULTS: The size of the defect or its caliber obtained from MRI in 6 patients with left to right shunt congenital heart disease was compatible with that observed in surgery (P = 0.924). Comparison of cine-MRI and CAG in 28 patients with complicated congenital heart disease showed that the diagnosis of 27 cases by cine-MRI was the same as that by CAG. CONCLUSION: Both MRI and cine-MRI play an important role in diagnosing infantile congenital heart disease.

Adolescent↗

PubMed2001

[Immunoelectron microscopic analysis of P-glycoprotein, p53, and Bcl-2 proteins expressions in lung cancer].

OBJECTIVE: To determine the ultrastructural localization of P-gp, p53 protein, and Bcl-2 protein in lung cancer cells, their relation to multidrug resistance and possible mechanisms of action. METHODS: Expression of P-gp, p53, and Bcl-2 proteins was examined in 8 NSCLC surgical specimens and 7 SCLC bronchoscopically biopsied specimens using postembedding PAG immunolabelling technique for electron microscopy. RESULTS: P-gp was detected on the cell membrane and the periphery of endoplasmic reticulum (ER). P53 protein was observed not only in the nuclei associated with heterochromatin but also in the cytosol. Bcl-2 protein immunoreactivity was associated with mitochondria and ER. P-gp, p53, and Bcl-2 were detected in 5(33%), 9(60%), and 4 (26.7%) out of the 15 samples examined, respectively. In 8 normal lung tissues, these three proteins were detected. Of 5 P-gp positive samples 4 were NSCLC, only 1 was SCLC after chemotherapy. CONCLUSION: P-gp, p53, and Bcl-2 proteins are detectable immuno-electron microscopically in lung cancer cells. No correlation in expression existed between of p53 and P-gp, nor did that between p53 and Bcl-2. The plasma membrane localization of P-gp supports its action as a transmembrane drug efflux pump. P-gp may play a role in MDR in lung cancer.

ATP Binding Cassette Transporter, Subfamily B, Mem↗

PubMed2001

[Mechanisms of arsenic trioxide-induced apoptosis in myeloma cells].

OBJECTIVE: To investigate the possible mechanisms of arsenic trioxide (As2O3)-induced apoptosis in multiple myeloma (MM) cell line, and the interactions between As2O3 and all-trans retinoic acid (ATRA) or interferon(IFN)-alpha. METHODS: Multiple myeloma cell lines RPMI 8226 and U266 were treated with As2O3 in combination with dithiothreitol (DTT), buthionine sulfoximine (BSO), ATRA and IFN-alpha. Cell viability was counted by trypan-blue exclusion. Apoptosis was assessed by cell morphology and flow cytometry. Mitochondrial transmembrane potentials (delta psi m) were measured by cellular rhodamine 123 staining intensity on flow cytometry. RESULTS: Glutathione depleting agent BSO at 1.0 mmol/L enhanced, while disulfide bond-reducing agent DTT at 0.2 mmol/L partially antagonized As2O3-induced decline of delta psi m and apoptosis in MM cells. ATRA also induced RPMI 8226 cell apoptosis, but it could not synergize with As2O3. On the other hand, As2O3-induced apoptosis did not occur in U266 cells. In addition, IFN-alpha itself did not inhibit the growth and viability of MM cells, nor did it influence the effects of As2O3 on MM cells. CONCLUSION: As2O3-induced apoptosis of RPMI 8226 multiple myeloma cells involves sulphyhydryl groups. ATRA and IFN-alpha do not synergize with As2O3 in the induction of apoptosis of MM cells.

Antineoplastic Agents↗

PubMed2001

[Estimation of insulin secretion rate in multiple sampled intravenous glucose tolerance test].

OBJECTIVE: To measure and calculate insulin secretion rate(ISR) in multiple sampled intravenous glucose tolerance test (FSIVGTT) according to the two compartment model and standard parameters of the kinetics of connecting peptide (C-P) in vivo. METHODS: 15 subjects with normal glucose tolerance and 11 type 2 diabetic patients took part in the study. Oral glucose tolerance test (OGTT) and FSIVGTT were performed. Glucose concentrations were measured by hexokinase method. Insulin (INS) and C-P concentrations were measured by radioimmunoassay. A computer procedure devised by our laboratory was used to calculate the value of ISR at each time point. RESULTS: In the control group, serum INS and C-P concentrations reached the peak at 4 min, the second peak was not obvious. In type 2 diabetes, only 5 subjects had a small peak within 4 min, two curves reached its peak between 60 and 80 min. In the control group, ISR reached its peak 2 min after injection of glucose, that was earlier than INS and C-P curves in time sequence (P = 0.000), the second obvious peak was shown at 22 min. In type 2 diabetes, an obvious but small peak of ISR was shown at 2 min and all the patients had this peak. CONCLUSIONS: It is necessary to take the process of metabolic clearance of INS and C-P into consideration, when blood concentration of INS and C-P were used as indexes to evaluate the secretion function of beta-cells. The ISR profile is more sensitive for investigation of the second phase of insulin secretion in the control group and the first phase in type 2 diabetes group.

Blood Glucose↗

PubMed2001

[Determination of ferulic acid absorbed into human serum from oral decoction of rhizoma chuanxiong and pharmacokinetic study of ferulic acid].

OBJECTIVE: To improve the HPLC method for determining ferulic acid(FA) in the human serum and to research FA clinical pharmacokinetics. METHOD: Serum concentrations of FA were determined by HPLC using methanol-water-acetic acid (40:59.7:0.3, v/v) as the mobile phase and the column packed with ODS (150 mm x 4.6 mm, 5 microns) as a fixed phase and the flow rate was 1.0 ml/min. FA was detected at 320 nm wave length. The internal standard was coumarin. The serum samples were treated by the water-boiling method. Peak of FA in serum was recognized by photodiode-array detection. RESULT: FA and internal standard were separated completely under the condition described as above. FA was linear in the range of (40.16-8032 ng/ml) (r = 0.9975). The CV was less than 10%, the average recovery was 100.3%, and the limit concentration in serum was 25.1 ng/ml. PK was in accordance with open bicameral model. CONCLUSION: This method can be applied to clinical pharmacokinetic study of FA.

Adult↗

PubMed2001

[Discussion of leigong 17 processing methods].

"Seventeen Methods" plays an important role in the field of processing medicinal herbs, and also makes a notable impact on modern ages. However, some problems among it are still existed. According to the origin of "17 Methods" and interrelating records, a simple description about its objections was given.

Drugs, Chinese Herbal↗

PubMed2001

[Effect of interferon-alpha on the prognosis of bone marrow transplantation in chronic myeloid leukemia].

OBJECTIVE: To investigate whether prior interferon alfa (IFN-alpha) treatment affects the outcome of allogeneic bone marrow transplantation. METHODS: The outcome of 85 patients with chronic myelogenous leukemia (CML) in first chronic phase received transplants from an HLA-identical sibling donor was analyzed. Of the 85 patients, 30 did not receive IFN-alpha, whereas 30, 15 and 10 patients received IFN-alpha 3 x 10(6) units 3-4 times per week for < 6, 6-12 and > 12 months before transplant combined with hydroxyurea and/or HA regimen (H: Harringtonine; A: Ara-C) respectively. RESULTS: Pretransplant IFN-alpha therapy for > or = 6 months was associated with an increased risk of severe (grade III-IV) acute graft-versus-host disease (GVHD) (P < 0.01) as compared to that in < 6 months or no IFN-alpha therapy. No influence of pretransplant IFN-alpha treatment on engraft, cGVHD, VOD, relapse and TRM (transplant-related mortality) was found. Survival rate of IFN-alpha therapy < 6 months group (90.0%) was higher than that of no IFN-alpha group (68.9%) or > 12 months group(60.0%) (P < 0.05). CONCLUSION: It was found that there was a trend towards an improved survival in the IFN-alpha < 6 months group. Pretransplant IFN-alpha therapy for > or = 6 months was associated with an increased risk of severe aGVHD.

Adolescent↗

PubMed2001

[Effects of calcium on biological characteristics of wheat seedlings under ultraviolet-B radiation stress].

Under simulated ultraviolet-B radiation (UV-B, 280-320 nm), effect of calcium on biological characteristics of wheat seedlings was studied with pot culture experiment. The results showed that the growth indexes of wheat seedlings on ground and underground in calcium concentration 2 mmol/L treated group were obviously reduced than that of wheat seedlings in control treated group (the descending range of 8.1%-36.1%) under UV-B stress (0.35 W/m2, 6 h per day, for 7 day). And the decreasing degree of growth indexes (3.2%-28.6%) for wheat seedlings in calcium concentration 6 mmol/L treated group were lower than in calcium concentration 2 mmol/L treated group. The experiment proved that calcium can mitigate the damage of ultraviolet-B radiation on wheat seedlings, and this effect was related to that calcium affected physiological and biochemical characteristics of wheat seedlings.

Calcium↗

PubMed2001

[Study on expression of P53mt protein in skin of patient with arseniasis caused by coal-burning].

OBJECTIVE: To study expression of P53mt protein in skin of patient with arseniasis caused by coal-burning. METHODS: According to pathological diagnosis, the patients were divided into carcinoma group (A group, 18 cases), pre-carcinoma group (B group 11 cases) and general pathological change group (C group, 39 cases). Immuno-histochemical technique of two steps was used to detect expression of P53 mt protein. RESULTS: Among A, B and C groups, the positive cell densities of P53mt protein were 38.07 +/- 29.00, 17.16 +/- 15.00 and 4.05 +/- 8.24 respectively, but no positive cell in normal group; there was statistical significance among A, B and C groups (A and C: P < 0.01, A and B: P < 0.01, B and C: P < 0.05); positive percentages of A, B and C groups were 88.89%, 72.73% and 25.64% (A and C: P < 0.01; B and C: P < 0.05), respectively, for difference of grade constitution, there was statistical significance (P < 0.01) among three groups; grade II and III made up 38.89% in A group, but 9.09% in B group and 2.56% in C group. CONCLUSIONS: P53mt gene play a certain role in the development of skin cancer from arseniasis caused by coal-burning; the detection of P53mt protein can show whether pathological changes of skin due to the disease is malignant or benign.

Adolescent↗

PubMed2001

[Vitreous surgery for proliferative maculopathy].

OBJECTIVE: To explore the indication of vitrectomy for the treatment of pre-macular proliferative disease, the surgical technique, results and the prevention and treatment of complications. METHODS: Forty-one eyes of 41 cases with different kinds of macular traction or macular pucker, including 12 cases of idiopathic epimacular membrane, 10 cases of macular hole complicated with epimacular membrane and 19 cases of macular pucker after reattachment surgery (no diabetic retinopathy or retinal detachment) were treated with vitreous surgery. The issue of this operation is to find out underlying fissures (an under-bridge) or weakly adherent sites between a membrane and the retina. Using flute-needle, intraocular pick, spade and vitreoretinal forceps, we can separate and peel the proliferative membrane and the posterior cortical vitreous from the retinal surface completely. RESULTS: In 41 cases, epimacular membrane was peeled successfully, and the normal anatomic structure of the macula was restored. Four cases had small amount of hemorrhage in the superficial layer of the retina in and after the operation, and all the other cases did not have any complications, such as retinal tear or detachment. Thirty-eight cases obtained higher visual acuity, and in 3 cases the visual acuity maintained at their pre-operative level. Metamorphopsia was significantly improved, even disappeared. The follow-up period was 3 months to 4 years. CONCLUSIONS: The therapeutic effects of vitreous surgery for treatment of various kinds of proliferative maculopathy are reliable. After the surgery, the visual acuity can be elevated, the symptoms can be ameliorated and the complications can be effectively controlled.

Adult↗

PubMed2001

[Relationship between soluble Fas ligand levels and complications after allogeneic bone marrow transplantation].

OBJECTIVE: To evaluate the implications of soluble Fas ligand (sFasL) in acute graft-versus-host disease (aGVHD) and discriminating symptoms of aGVHD from those of infection. METHODS: Plasma levels of sFasL were assessed in 84 plasma samples from 13 patients after allogeneic BMT using a sandwich enzyme-linked immunological assay (ELISA). Plasma sFasL levels of the patients before BMT and at different time points in the post-BMT period were measured. The results were analysed for correlation with aGVHD and infections. RESULTS: Plasma sFasL levels were significantly higher in patients with grade II - IV aGVHD than that in those with grade 0 - I aGVHD (P = 0.02). There was no statistic difference in plasma sFasL levels between the infectious and non-infectious patients. In the seven grade II - IV aGVHD patients, the plasma sFasL levels pre-BMT were much lower than that in the six grade 0 - I aGVHD patients. CONCLUSIONS: sFasL may be useful for the diagnosis of aGVHD and for differentiating aGVHD from other BMT related complications. The high level of plasma sFasL pre-BMT may be of importance in decreasing the occurrence of aGVHD after BMT.

Adolescent↗

PubMed2001

[Clinical significance of hemostatic molecular markers in acute leukemia].

OBJECTIVE: To study the changes of hemostatic molecular markers in acute leukemia (AL) for elucidating their clinical significance. METHODS: A series of hemostatic molecular markers, including TF, TFPI, TAT, PAP, u-PA and u-PAR were measured in 82 acute leukemia. RESULTS: The plasma levels of TF, TAT, u-PA and u-PAR were elevated significantly at diagnosis in all AL patients tested, while TFPI, PAP did only in AML. After treatment, TF and TAT remained high in AML, u-PA and u-PAR were still high in un-remission patients. PAP and u-PA remarkably elevated in patients with severe hemorrhage. CONCLUSIONS: There existed hemostatic abnormality as well as hyperfibrinolysis, which varied with leukemia types and was ameliorated with clinical improvement. The measurement of TF, TAT and PAP may provide useful information for the diagnosis of DIC. u-PA and u-PAR may be considered as a useful indicator for prognosis. Patient with severe hemorrhage should be treated with antifibrinolysis drugs. The prevention of hypercoagulability after treatment should be kept in mind.

Acute Disease↗

PubMed2001

Mutagenesis of N-terminal Amino Acid Residues in beta-subunit of Glutaryl-7-amino-cephalosporanic Acid Acylase C130.

In many GL-7ACA acylases, the first Ser residue at the N-terminal of beta-subunit is the catalytic center. In order to investigate relationship between the N-terminal structure and catalytic activities, peptide replacement and site-directed mutagenesis were performed at the N-terminal of beta-subunit of GL-7ACA acylase C130. When the N-terminal 8 amino acid residues of C130 were replaced by the corresponding sequence of penicillin acylases PAC and PGA, respectively, the first mutant B8PAC lost the activity of the acylase, and the second mutant B8PGA had lower activity with the K(m) value increasing from 0.44x10(-3)mol.L(-1) to 0.55x10(-3) mol.L(-1), and the k(cat) decreasing from 4.92 s(-1) to 1.64 s(-1). Although the substitution of Trp (beta4) by Tyr did not change the K(m) value, the k (cat) decreased to 2.29 s(-1). When the Trp was substitued by Leu, both the K ( m ) and k ( cat ) values decreased. Compared with the wild type, mutations of Ser (beta3) to Met, Ala and Cys caused decrease of K(m) values by 52.27%, 43.18% and 38.64%, respectively. Mutation of Asn (beta2) to Gln caused the K ( m ) value being increased by 5-fold, and k ( cat ) decreased by 10-fold. These results suggested that the N-terminal amino acid residues of beta-subunit in GL-7ACA acylase C130 are important for enzyme function.

Journal Article↗

PubMed2001

[An experimental study on bone marrow purging in vitro for patients with chronic myelogenous leukemia].

OBJECTIVE: To investigate the effect of IL-2, IFN and bcr-abl AS-ODN on bone marrow purging in vitro for patients with chronic myelogenous leukemia (CML). METHODS: By amplifying in vitro clone forming culture, and purging the bone marrow of 5 CML patients through different combined use of IL-2, IFN and bcr-abl AS-ODN, we compared the effects of 4 combined regimens. RESULTS: It was found that the suppressive effects of the 4th regimen combining IL-2 (800 U/ml), IFN (800 u/ml) and bcr-abl AS-ODN (30 micrograms/ml) on leukemia cell clone forming unit (CFU) were significantly different from the suppressive effects of the other 3 regimens (P < 0.01). With the use of the 4th regimen, the GM-CFU and L-CFU survival rates were 26.91% and 3.49% respectively (P < 0.01). CONCLUSION: The combined regimen of IL-2 (800 U/ml), IFN (800 u/ml) and bcr-abl AS-ODN (30 micrograms/ml) has good effect on selective CML bone marrow purging in vitro and can be used in clinical purging of CML bone marrow.

Bone Marrow Cells↗

PubMed2001

[Clindamycin soluble suppository-production and its efficacy in treating bacterial vaginosis].

OBJECTIVE: To make the soluble suppository of clindamycin which is an effective drug for treatment of Bacterial vaginosis and to treat patients in clinical trials. METHODS: Outpatients who had vaginal infections from Sept. 1998 to Jan. 1999 were investigated. The diagnoses of bacterial vaginosis were made according to Amsel's criteria and 80 patients were included in this study. They were divided into two groups to be treated respectively by metronidanole suppository and clindamycin suppository which were made by our hospital pharmacy. RESULTS: The cure rate of metronidanole was 87.5% and that of clindamycin 90.3%; No significant difference was noted between the two groups(P > 0.05). The new clindamycin suppository was not irritant and it was easy to dissolve in vagina. There was no discontinued treatment because of side-effect. CONCLUSION: The clindamycin suppository does not irritate the mucosa of vagina, it is an efficacious remedy for bacterial vaginosis.

Administration, Intravaginal↗

PubMed2001

[Study of bacteriology on local application of Tinidazole stilus in treatment for periodontitis].

OBJECTIVE: The study was designed to evaluate the antiseptic efficacy of Tinidazole stilus against periodontal pathogen. METHODS: A total of 41 patients with periodontitis were included in this study. 26 of them were treated by Tindazole stilus and other 15 cases were treated by metronidazole stilus as controls. The prevalence of subgingival bacteria from both group were examined. RESULTS: The eliminate rates for Black pigmented anaerobic rod (Porphyromonas gingivalis, Prevotella intermedius, Prevotella melaninogenicus), Fusobacteriums, Prevotella oris, Prevotella oralis, Eubacterium and Actinomyces were 95.8% 94.1%, 83.3% and 76.5%. The anti-bacterial efficiency of Tinidazole against periodontal pathogen was significantly higher than that of metronidazole (P < 0.05). CONCLUSION: It appears to be effective to use Tinidazole stilus as local application against periodontal pathogens.

Administration, Topical↗

PubMed2001

[Clinical analysis of 62 cases laryngeal papilloma in children].

OBJECTIVE: To investigate the clinical features of laryngeal papilloma in children. METHOD: In a group of 62 patients with laryngeal papilloma in children, the tumors of 28 patients were cut under the direct laryngoscope, 34 patients were treated with laryngeal micro-laser operation. RESULT: The post-operation followup ranged from 2 to 5 years, the cure rate of 2 years was 51.61%. CONCLUSION: The clinical features of this disease include rapid development, a large lesion, and it is often found in the infraglottic cavity. Furthermore, we believe that in order to eliminate the tumors more accurately and decrease recurrence, micro-laser surgery and the use of interferon is very necessary.

Adolescent↗

PubMed2001

[Observation on the changes in nasopharyngeal carcinoma patients' olfactory before and after radiotherapy].

OBJECTIVE: Detecting nasopharyngeal carcinoma (NPC) patients' olfactory before and after radiotherapy for investigating the infection of radiotherapy on these patients' olfactory. METHOD: 100 NPC patients' olfactory were detected before the start of radiotherapy and after the termination of therapy in 3, 6, 12, 24 and 36 months. RESULT: Patients' smell acuity decreased sharply 3 months after therapy, but varying degrees of recovery were noted in 6 and 12 months. However their smell acuity became worse in the following time. CONCLUSION: NPC patients' smell acuity can be profoundly affected by radiotherapy. And after radiotherapy, their smell acuity will decrease wave-likely and cannot fully recover.

Adult↗