Biomedical subjects

X Huang

Publications and source records attributed to X Huang.

At least 343 records · Page 19Linked to original sources

PubMed1999

[Observation of the long-term serum insulin level in women with gestational diabetes history].

OBJECTIVE: To determine beta-cell's function by measuring blood glucose level and serum insulin level in women who had history of gestational diabetes mellitus (GDM), but had no long-term diabetes. METHODS: 30 cases in GDM history group, 29 cases in abnormal oral glucose tolerance test(OGTT) history group, and 38 cases in control Group were followed up to undergo fasting blood sugar test and 75 g glucose load test, and to measure their insulin levels. All the cases had no long-term diabetes. RESULT: (1) the blood glucose levels at 2 hour in GDM history group and abnormal OGTT history group were higher than that in control group especially the former, which was very significantly higher (P < 0.001). (2) The insulin at 2 hours in GDM history group was higher than that in control group (P = 0.00067). CONCLUSION: Though women with GDM history do not have long-term diabetes, insulin resistance still exists, postings a possible sign for future diabetes attack.

Adult↗

PubMed1999

[A study of plasma terminal SC5b-9 complement complex in patients with pregnancy induced hypertension].

OBJECTIVE: To study the relationship between complement SC5b-9 complex and damage of vascular endothelial in pregnancy induced hypertension (PIH). METHODS: Plasma SC5b-9, Serum circulate immunocomplex (CIC), fibronectin (Fn) levels were determined with ELISA in 47 cases of PIH (study group 1) and 15 cases with potential risk factors of PIH (study group 2) and 40 cases of normal pregnancies (control group). RESULTS: SC5b-9 level increased and CIC level decreased significantly in study group 1 and study group 2 than that in control group (P < 0.01). The Fn level increased in moderate and severe PIH cases than that of mild PIH and control group (P < 0.01). The positive rates of SC5b-9 in study group 1 and study group 2 were significantly higher than those of the control group (P < 0.01). The positive rate of Fn in moderate and severe PIH cases significantly higher than those of mild PIH cases and control group (P < 0.01). CONCLUSION: Complement was activated by classical pathway. Terminal SC5b-9 complement complex play an important roles in the pathogenesis of PIH and has close relationship with vascular endthelial damage.

Adult↗

PubMed1999

Immunological studies on the cellular phenotype involved in corneal allograft rejection.

OBJECTIVE: To investigate the cellular phenotype involved in corneal allograft rejection using wholemounts analysis. METHODS: Corneal transplantation was performed between Sprague Dawley (SD) and Wistar rats. Corneal wholemounts were prepared from control rats and those after corneal transplantation on day 7 and 12. Immunohistochemical stain was performed on these wholemounts using monoclonal antibodies to transforming growth factor beta 1(TGF-beta 1), CD3, CD4, CD8, B lymphocytes, macrophages, dendritic cells and major histocompatibility complex (MHC) class II antigen. RESULTS: Corneal allograft rejection started on day 7 and reached its maximum from 10 to 14 days after corneal transplantation. Presence of TGF-beta 1-, CD3-, CD4-, CD8-, MHC class II-positive cells, macrophages and dendritic cells were noted at the limbus of both SD rats and Wistar rats. No positive cell was present in the central cornea of normal rats. All positive cells but B lymphocyte were noted in large numbers in the cornea after corneal allograft transplantation. Marked staining for TGF-beta 1 was noted during graft rejection. CONCLUSION: The corneal wholemounts technique provides a good visualization for the cellular phenotype involved in corneal allograft rejection. A variety of cells including TGF-beta 1, CD3, CD4, CD8, MHC class II antigen positive cells, macrophages and dendritic cells are involved in corneal allograft rejection. TGF-beta 1-positive cell might be an important immunosuppressive factor after corneal transplantation and also involved in the induction of fibrosis.

Animals↗

PubMed1999

[Regulation of arsenic trioxide-inducing apoptosis].

OBJECTIVE: To explore the relationship among intercellular -SH, caspase, retinoic acid (RA) and arsenic trioxide(As2O3)-induced apoptosis. METHODS: The in vitro effect of different thiols compounds, RA and caspase inhibitors on As2O3-induced apoptosis in NB4 and HL-60 cells was studied. RESULTS: 1. NAC completely blocked, BSO potentiated while MTG, BAL had no effect on As2O3-induced apoptosis. 2. Z-VAD.fmk blocked while Y-VAD.fmk had no effect on As2O3-induced apoptosis. 3. RA and As2O3 showed synergism in HL-60 cells, while showed antagonism in NB4 cell. CONCLUSIONS: 1. As2O3 binds with intracellular -SH, changes signal transduction, selectively activates caspase and causes apoptosis. 2. The regulating effect of RA on As2O3-induced apoptosis depends on cell types.

Acute Disease↗

PubMed1999

Angiotensin I-converting enzyme gene polymorphism in Chinese patients with obstructive sleep apnea syndrome.

OBJECTIVE: To investigate the relationship of an insertion/deletion (I/D) polymorphism of the angiotension-converting enzyme (ACE) gene to obstructive sleep apnea syndrome (OSAS) patients and the control subjects. METHODS: Genomic DNA was extracted from blood samples and amplified by polymerase chain reaction (PCR). PCR primers flanked the polymorphic region in intro 16 of the ACE gene. RESULTS: OSAS patients had significantly higher frequencies of I/I genotype and insertion allele of the ACE gene as compared with the control subjects in Chinese population. The OSAS patients with I/I genotype had significantly longer apnea time, lower minimum SaO2 and greater AHI than the OSAS patients with I/D genotype. CONCLUSION: These results indicate that the I/I genotype and I allele are a risk factor for OSAS in Chinese.

Adult↗

PubMed1999

External valvuloplasty technique in deep venous valve insufficiency of the lower limbs.

OBJECTIVE: To evaluate the therapeutic effects of external valvuloplasty technique in deep venous valve insufficiency of the lower limbs. METHODS: External valvuloplasty of the femoral vein valve was performed in 30 patients(41 limbs) with deep venous valve insufficiency of the lower limbs. The patients ranged in age from 16 to 69 years (mean +/- s, 50.8 +/- 9.2). External valvuloplasty of the first pair of superficial femoral vein valve was carried out in all limbs. External valvuloplasty of the second pair of superficial femoral vein valve was added in 7 limbs and valvuloplasty of the common femoral vein valve in 6 limbs. RESULTS: Venous claudication, swelling, aching disappeared in 78.0% limbs (32), obvious improvement was seen in 22.0% (9), and varicosis disappeared in all limbs. Ulcer healing was observed in 80% (8/10) limbs. Deep venous valve sufficiency was shown in 90.2% limbs, and reflux I degree-II degree in 4 by color Doppler. CONCLUSION: External valvuloplasty is an effective method to treat deep venous valve insufficiency of the lower limbs.

Adolescent↗

PubMed1999

Classification and digital subtraction angiography evaluation of carotid cavernous fistulas.

OBJECTIVE: To investigate the role of digital subtraction angiography (DSA) in evaluation of carotid cavernous fistulas, and to initiate a new classification for carotid cavernous fistula. METHODS: Cerebral angiography and selective external and internal carotid angiography were carried out in 56 patients who were classified into 5 types according to their hemodynamic features. RESULTS: The types were A (38 patients), B (4), C (10), D (2), and E (2). One of the 38 direct fistulas was located at C1 of the cavernous segment. Fistulas at C2 were observed in 10 patients, at C3 in 4, at C4 in 10 and at C5 in 5. The anterior communicating arteries were patent and well compensated in 33 of the 56 patients. The posterior communicating arteries were patent and well compensated in 39 patients. Both communicating arteries were well compensated in 31 patients. Fifty of the 56 patients were drained by ophthalmic veins, 47 by inferior petrous sinuses, 18 by cortical veins, and 8 to the posterior fossa region. CONCLUSIONS: Digital subtraction angiography was helpful in localization of the rents in carotid cavernous fistulas, in evaluation of function of Willis circles and provided valuable information for treatment of carotid cavernous fistulas. Carotid cavernous fistulas were divided into 5 categories: type A, B, C, D and E, among which type E was not previously described elsewhere.

Adolescent↗

PubMed1999

A brief history of antibiotic science.

As the greatest achievement in the history of microbiology, the story of antibiotic is an extremely inspired chapter in its history. Like the developmental process of other sciences, antibiotic reveals a history of first accumulating experience, followed by the stage of interpretation of the phenomena as other experimental study. Meantime, there is no end to the discovery of new antibiotics. Currently, the scope and province of antibiotic research is expanding both in its profundity and coverage.

Anti-Bacterial Agents↗

PubMed1999

Zhang Binglin and traditional Chinese medicine.

As an important thinker and scholar as well as a famous physician in modern Chinese history, Zhang Binglin extensively read medical books, ancient and modern, and absorbed modern western medical knowledge. He achieved great attainments in the studies on Shang han lun and in the confluence of Chinese and western medicine. His achievements include the "hypothesis of Shang han in a broad sense", "Conception of Six - channels - Six - Departments", questioning on spreading the shang han disease by channels, assessment of commentaries on shang han in successive ages, theory on "Triple - jiao - lymphatic system", "elimination of five - element theory" and discourse on damp - warmness diseases. As the "teacher of reform in modern TCM", Zhang's unique idea and achievements established his position in the history of modern Chinese medicine.

China↗

PubMed1999

[Development of the diagnosis and treatment of glaucoma in China].

Diagnosis and treatment of glaucoma is one of the greatest achievements in the development ophthalmology. The process of achieving is also a very inspiring chapter in its history. As histories of other sciences, the development of diagnosis and treatment of glaucoma is achieved through the following process: After accumulation for many years, then it entered the stage of explaining the phenomenon with the development of other sciences. Finally, its situation is determined by experiment and being widely applied in clinical practice. Meanwhile, there is no end for the findings of new diagnostic and therapeutic methods. More achievements will surely be made for developments in relevant sciences and increasing needs in clinical practice.

China↗

PubMed1999

[Chronic obstructive pulmonary disease and pulmonary structural remodeling].

OBJECTIVE: To understand the pathological characteristics of the lungs in 20 autopsy cases of chronic obstructive pulmonary disease(COPD) complicated with cor pulmonale and its relationship with structural remodeling of the lungs. METHODS: The lungs of 20 autopsy cases of COPD complicated with cor pulmonale were fixed in 10% formalin or by low pressure 18.4-33.4 mm Hg perfusion with 10% formalin. The lungs were cut sagitally into large slices with 2 cm in thickness, and the percentage of the emphysematous areas was calculated by point counting. The pathological characteristics of bronchioles and the stromal fibrosis area were examined microscopically and quantitatively using a latticed micrometer (Zeiss). The distribution and the ratio of type I to type III collagen in the lung tissue were determined using a sirius red-polarizing microscopy-morphmetry method. RESULTS: Of the 20 cases of COPD, irregular and saccular emphysema were the most frequent (17/20). 10 cases had moderate and high degree of emphysema, and 6 cases showed hypertrophy of the right ventricle (heart weight > 400 g). The mean area of stromal fibrosis auounted for total area of the examined lungs was (27.1 +/- 2.9)% and control group (12.0 +/- 1.8)%. The ratio of the area of the type I collagen to type III collagen was 5.6:1 (control group 1.3:1). All 1504 bronchioles showed chronic inflammation, 38% of which revealed acute exacerbation, and 53% of which showed lumen stenosis and fibrotic obstruction. CONCLUSIONS: Acute and chronic bronchiolitis and peri-bronchiolitis are the essential pathological changes in COPD, and also are the key link of the structural remodeling of the pulmonary tissue and vessels.

Bronchi↗

PubMed1999

[Direct determination of serum content of ferulic acid treated with boiling water in HPLC in volunteers after oral Administration of Coronary Heart No. II decoction].

OBJECTIVE: To develop a new method for clinical pharmacokinetic detection of chemical component of Coronary Heart NO. II in serum after oral administration in healthy person. METHODS: Content of ferulic acid (FA) in serum, which had been treated with boiling water bath, was determined directly by high performance liquid chromotography (HPLC) and spectrophotometer. After qualitative detection performed by using Bi- and tri-dimensional HPLC and ultraviolet spectrophotometer, the quantitative detection was determined with the internal standard (coumarin). RESULTS: When eluted by mobile phase of a mixture of methanol, acetic acid and water (38:0.3:61.7 in ratio), and stationary phase of C18(ODS2), column (150 mm x 4.6 mm, 5 microns), the minimal detectable limit of free methanol FA was 6 ng (N/S = 3), and the minimal detectable serum FA concentration, after 10 min of boiling water bath, was 20.2 micrograms/L, with a linearity of 33.7-2156.8 micrograms/L, r = 0.9993. The mean recovery of serum FA was (93.59 +/- 2.36) micrograms/L. The RSD within day and day-to-day were all less than 8.44%. CONCLUSION: In comparing with sample Preparation of acetonitrile, this method is simple, rapid, sensitive, cheap, accurate, specific, reproducible and no toxic.

Adolescent↗

PubMed1999

[The relationship between obstructive sleep apnea and gastroesophageal reflux and the effect of antireflux therapy].

OBJECTIVE: To evaluate the relationship between gastroesophageal reflux (GER) and obstructive sleep apnea (OSA) and the effect of combined therapy with cisapride and omeprazole. METHODS: 24-hour esophageal pH and manometric monitoring and polysomnography (PSG) were performed in 18 patients with snoring, daytime sleepiness and acid reflux, heartburn and regurgitation. Cisapride 10 mg qid and omeprazole 20 mg q12h were given to patients with GER and OSA. these tests were repeated on the 7th day. RESULTS: 7 of the 18 patients with OSA had severe GER. All except one had daytime GER as well as nocturnal GER. Nocturnal recordings were analyzed and it was found that esophageal pH below 4.0 was frequently followed by swallow action (58.6%, control = 20.6%, P < 0.05), gross body movement (18.6%, control = 4.8%, P < 0.05) and arousal (26.8%, control = 16.8%, P < 0.05). The mean lowest esophageal pressure [(-15.2 +/- 9.6) mmHg] before and [(-18.6 +/- 10.1) mmHg] after the drops of pH were significantly lower than that during control periods [(-10.6 +/- 8.1) mmHg, P < 0.005]. After antireflux therapy, there was dramatically improvement in total apnea time [from (52.7 +/- 27.1) to (21.6 +/- 21.2) minutes, P < 0.002)] and apnea hypopnea index (AHI) [from (38.9 +/- 17.6) to (15.2 +/- 12.3) No./h, P < 0.002]. CONCLUSION: GER is a predisposing factor for OSA, they may coexist and aggravate each other. Combined therapy with cisapride and omeprazole is a new, simple and effective method for GER with OSA.

Adult↗

PubMed1999

[Extensive HLA class II studies in Chinese narcoleptic patients].

OBJECTIVE: Narcolepsy is a debilitating, lifelong sleep disorder. Its familial occurrence suggests that genetic factors may be of importance in the etiology. Narcolepsy is a very rare disease among Chinese, thus it was of interest to study the association of narcolepsy with the HLA system in Chinese narcoleptic patients. METHODS: To explore the role of HLA-DRB genes in the development of narcolepsy, we studied 10 narcoleptic patients and 50 race matched controls in whom HLA-DR typing was performed by the method of DNA amplification with sequence-specific primers (PCR-SSP). RESULTS: All narcoleptic patients were found to be of DRB1 * 1501 and DRB5 * 0101 genotype. CONCLUSION: These results indicate that HLA-DRB1 * 1501 is a better primary candidate susceptibility gene for narcolepsy in Chinese.

Adult↗

PubMed1999

[External valvuloplasty for deep venous valve insufficiency of legs].

OBJECTIVE: To study and evaluate the therapeutic effects of external valvuloplasty for deep venous valve insufficiency of the legs. METHODS: External valvuloplasty of the femoral vein valve was performed in 26 patients (37 legs) with deep venous valve insufficiency of the legs. Among them, 12 were male and 14 were female. These patients ranged in age from 16 to 69 years (x +/- s, 50.8 +/- 10.0). External valvuloplasty of the first pair of the superficial femoral vein valve was carried out in all the legs. External valvuloplasty of the second pair of the superficial femoral vein valve was added in 7 legs and valvuloplasty of the common femoral vein valve in 6 legs. RESULTS: Venous claudication, swelling, aching disappeared in 81.1% patients (30/37), obviously improved in 18.9% (7/37) and varicose veins disappeared in all legs. Ulcer healing was seen in 7/9 patients. Deep venous valve sufficiency was demonstrated in 89.2% patients (33/37), and reflux I degrees - II degrees in 4 legs by color Doppler. CONCLUSIONS: External valvuloplasty is effective in treating deep venous valve insufficiency of the legs.

Adolescent↗

PubMed1999

[Slow transit constipation treated by surgery: analysis of 17 cases].

OBJECTIVE: To explore the operative indications, prognosis, and possible pathogenic factors of slow transit constipation (STC). METHODS: 17 cases with STC who had undergone colectomy were analysed. RESULTS: Bowel movement frequency was once per 4 to 14 days in all of the patients, who had had long-term laxative abuse. Delayed colonic transit was demonstrated in all patients, 82.4% of them were accompanied by outlet obstructive constipation (OOC) and 50% showed abnormal sex hormones. Reduced numbers of argyrophilic neurons and vacuolar degeneration of axons were demonstrated at the myenteric plexus pathologically. Three patients had adhesive ileus postoperatively and one patient suffered from difficult evacuation again 5 years after the operation. CONCLUSIONS: Colectomy is highly effective in alleviating symptoms in patients with STC, and pelvic floor disorder of OOC should be treated simultaneously.

Adolescent↗

PubMed1999

[Liver metastasis in colorectal cancer and tumor angiogenesis].

OBJECTIVE: To investigate the association between tumor angiogenetic activity and metastatic potential of colorectal cancer (CRC) retrospectively. METHODS: The microvessel density (MVD) and the level of vascular endothelial growth factor (VEGF) expression were examined immunohistochemically from 61 specimens obtained by surgical resection of CRC. RESULTS: MVD was (27.0 +/- 8.4) and the expression of VEGF was (59.75 +/- 12.36)%. Both were significantly higher in the group of liver metastasis, and in the group of lymph node metastasis (P < 0.01, P < 0.05). Survival analysis indicated a significant difference between increasing MVD group and the control group (P < 0.01). CONCLUSIONS: VEGF-mediated angiogenesis plays an important role in liver metastasis of CRC, and MVD appears to be a new prognostic factor for the prediction of outcome in patients with CRC.

Colorectal Neoplasms↗

PubMed1999

[Lymphoscintigraphy: evaluating lymphatic spread of rectal cancer].

OBJECTIVE: To investigate lymphatic drainage of rectal and lymphatic spread of rectal cancer. METHODS: Rectal endoscopic lymphoscintigraphy was performed in 37 volunteers and 16 patients. One millilitre dextran marked with (99m)Tc was injected bilaterally into submucosa of the rectum through rectal endoscopy. 1, 2, 3 hours after the injection, rectal lymphoscintigraphy was registered with a computerized gamma camera (TOSHIBA GCA901A). RESULTS: Lymphoscintigraphy in control subjects showed upward epirectal and pararectal nodes. The average number of the nodes was 12 three hours after the injection. Compared with histological node examination in all patients operated upon for rectal cancer, rectal endoscopic lymphoscintigraphy showed a sensitivity of 97.1%, a specificity of 75.0%, an overall accuracy of 87.5%, a false positivity of 25.0%, and false negativity of 8.3%. CONCLUSION: The method is better for evaluating lymphatic spread of rectal cancer before operation.

Adult↗