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Biomedical subjects

X H Huang

Publications and source records attributed to X H Huang.

At least 37 records · Page 2Linked to original sources
PubMed1998

Mutation C677T of methylenetetrahydrofolate reductase gene is not associated with coronary artery disease, but possibly with albuminuria, in type 2 diabetic patients.

The missense mutation in the 677th nucleotide (C677T) of methylenetetrahydrofolate reductase gene causes substitution of valine (V) for alanine (A) resulting in three genotypes VV, VA and AA. The VV genotype causes hyperhomocysteinemia and may be a risk factor for coronary artery disease. We determined genotypes by polymerase chain reaction and subsequent restriction fragment length analysis and compared them in 84 patients with type 2 diabetes and in 115 non-diabetic subjects with and without coronary disease. Fractional urinary excretion rate of albumin was assessed by nephelometry. The VV, VA, and AA frequencies in the diabetic and in the control groups were 0.095, 0.357, 0.548 and 0.061, 0.417, 0.522, respectively (p = NS, diabetic vs. controls, chi2 test). Genotype frequencies did not differ in either diabetic or control subjects between those with or those without coronary disease (chi2 test). The fractional urinary excretion rate of albumin (mean +/-SD) in diabetic patients with the VV genotype i.e. 1.59 +/-0.71 was lower (Kruskall-Wallis test p = 0.002) than in the other genotypes i.e. VA 5.98 +/-9.75 and AA 3.75 +/-4.77, respectively (post-hoc Mann-Whitney test VV vs. VA p = 0.005 and VV vs. AA p = 0.054, respectively). We found that in patients with type 2 diabetes the methylenetetrahydrofolate reductase VV genotype was associated with a low urinary albumin excretion but not with coronary artery disease or diabetes per se.

Adult↗
PubMed1997

Fast 5-hour determination of angiotensin-converting enzyme genotypes from blood by SDS-PAGE using PhastSystem.

Polymorphism in the angiotensin-converting enzyme (ACE) gene has been shown to correlate with circulating ACE concentrations in plasma, and also to be an independent risk factor in the development of many cardiovascular diseases. However, methods available today for ACE genotyping are still laborious and time-consuming. Here we report a sensitive, simple and non-isotopic procedure with commercially available gels for the identification of ACE insertion/ deletion polymorphism. This technique includes rapid extraction of the DNA by the QIAamp Blood Kit prior to polymerase chain reaction, followed by sodium dodecylsulphate polyacrylamide gel electrophoresis using the PhastSystem (Pharmacia). The procedure can be accomplished in five-hours from drawing the blood samples to the final result.

Aged↗
PubMed1997

[The study on the epidemiological features and risk factor of Chlamydia trachomatis reproductive tract infection (CTRTI) among 664 married women at reproductive age in rural areas].

The infection rate and risk factors of CTRTI among 664 married women at reporductive age in rural areas in Chengjiang county, Yunnan province were investigated. Specimens of cervical swab were detected for CT antigen using Chlamydia EIA(Syva co., USA). The selection of major risk factors was through Logistic regression and chi 2-test. The results showed that the overall infection rate of CTRTI was 5.0%, with 60.6% of these cases asymptomatic. The major risk factors include: spouse who has symptoms of RTIs; history of others vaginitis; divorce; habit of washing face, feet and vulva with the same wash basin; age of menarche; amenorrhoea during lactation; low education and living in poverty.

Adult↗
PubMed1995

Terpendoles, novel ACAT inhibitors produced by Albophoma yamanashiensis. I. Production, isolation and biological properties.

A series of new acyl-CoA: cholesterol acyltransferase (ACAT) inhibitors termed terpendoles were isolated from the culture broth of a fungal strain FO-2546 which was proposed to belong to a new genus designated as Albophoma yamanashiensis. Among four structurally related terpendoles, terpendole C showed the most potent ACAT inhibitory activity with an IC50 value of 2.1 microM in an in vitro enzyme assay, followed by terpendoles D (IC50: 3.2 microM), A (15.1 microM) and B (26.8 microM). Evaluation of their ACAT inhibition in the cell assay using J774 macrophages indicated that terpendole D exhibited the highest specificity (cytotoxicity vs. ACAT inhibition) among microbial ACAT inhibitors we discovered so far.

Animals↗
PubMed1995

Terpendoles, novel ACAT inhibitors produced by Albophoma yamanashiensis. II. Structure elucidation of terpendoles A, B, C and D.

Structures of terpendoles A, B, C and D, novel acyl-CoA: cholesterol acyltransferase (ACAT) inhibitors, were determined by spectroscopic studies. All terpendoles consist of diterpene and indole moieties in common. Terpendoles A, C and D possess an additional isoprenyl unit via oxygen atom(s) of their diterpene moieties. The relative stereochemistries of terpendoles C and D were confirmed by NOE experiments and X-ray crystallographic analysis.

Anti-Bacterial Agents↗
PubMed1994

[Relation between cesarean section rate and perinatal mortality rate].

In order to evaluate the effect of cesarean section on reduction of perinatal mortality rate (PMR). All cases delivered in Beijing Obstetrics and Gynecology Hospital from Jan. 1, 1980 to Dec. 31, 1992 were analysed. The total number of delivery in study period was 74,199. Number of cesarean section delivery was 18,557. The cesarean section rate was 25.0%. The total number of perinatal baby was 74,914. The number of perinatal death was 1,140. PMR was 15.2%. The 13 years were divided into three periods to compare the cesarean section rate and PMR. The 1st period was from 1980 to 1984. The 2nd period was from 1985 to 1988. Years from 1989 to 1992 were 3rd period. The cesarean section rate was increasing year by year. It was significant since 1985. On the other hand, the PMR was marked decreasing since 1983. But there was no continually decrease in 2nd period. In 3rd period trend of decreasing PMR was shown. There was no significant difference in PMR especially in neonatal mortality rate (NMR) between cesarean section group and vaginal delivery group in 1st period. In 2nd period the PMR and NMR of cesarean section group was higher than that of vaginal delivery group (P < 0.01). In 3rd period it was lower in cesarean section group (P < 0.05). Main causes of neonatal deaths in cesarean section group were congenital abnormalities and respiratory diseases. In 2nd period pulmonary hemorrhage, hyaline membrane disease were marked higher in cesarean section group. It was related to more premature and low birth weight babies in this group.(ABSTRACT TRUNCATED AT 250 WORDS)

Cesarean Section↗
PubMed1994

[Puerperal infection of ureaplasma urealyticum].

Using indirect agglutination test (IAT), ELISA and metabolic inhibition test (MI), serum specific IgM antibodies to ureaplasma urealyticum (Un) was investigated in 160 women of late pregnancy during the period of 1991-1992. 81 cases (50.6%) were found positive. 65 out of 81 positive mothers had umbilical blood samples examined and placenta tissues cultured. Specific IgM was detected in the umbilical blood of 7 women (10.8%), and ureaplasma urealyticum was isolated from 6 placenta (9.2%). Among Un IgM positive women, 28 (34.6%) were complicated with puerperal fever, and 30 newborns suffered from neonatal pneumonia and fever, which were significantly higher than that in the Un IgM negative group (P < 0.05). This study indicated that ureaplasma urealyticum might be one of the important pathogens for puerperal and neonatal infection.

Adult↗
PubMed1993

[Functional changes of hypothalamic-pituitary target gland axes and their clinical significance].

We examined the function of hypothalamic-pituitary target gland axes in 88 cases with epidemic hemorrhagic fever (EHF). It was found that all the three endocrine axes i.e. hypothalamic-pituitary-adrenocortical axis, hypothalamic-pituitary-thyroid axis and hypothalamic- pituitary-gonadal axis showed some functional impairment. In acute phase there were increased plasma ACTH, FSH and LH levels, which might be attributed to stress reaction in EHF patients. Most cases showed low or weak response of TSH to TRH and delayed response of LH to LHRH; some of the cases did not respond to 1mg of glucagon. These manifestations might be related with poor reserve of anterior pituitary or to disordered regulation of endocrine axes. The increased plasma cortisol and estradiol and the decreased plasma T3, T4 as well as testosterone during acute phase may have important clinical significance. Hence we suggest that in any case glucocorticosteroids should not be abused and that in severe patients thyroxine and durabolin should be administered to improve immunity and hepato-renal function.

Adult↗
PubMed1992

Glisoprenins, new inhibitors of acyl-CoA: cholesterol acyltransferase produced by Gliocladium sp. FO-1513. I. Production, isolation and physico-chemical and biological properties.

Gliocladium sp. FO-1513 was found to produce novel inhibitors of acyl-CoA: cholesterol acyltransferase (ACAT). Two active compounds, designated glisoprenins A and B, were isolated from the culture broth of the producing strain by a conventional method. The IC50 values of glisoprenins A and B for ACAT activity were 46 and 61 microM in an enzyme assay using rat liver microsomes, and 1.2 and 0.57 microM in a J774 macrophage assay, respectively.

Animals↗
PubMed1992

New cyclodepsipeptides, enniatins D, E and F produced by Fusarium sp. FO-1305.

New cyclodepsipeptides named enniatins D, E and F were isolated from the culture broth of Fusarium sp. FO-1305 as inhibitors of acyl-CoA:cholesterol acyltransferase (ACAT). The respective structures of enniatins D, E and F were determined to be cyclo[D-alpha-hydroxyisovaleryl(D-Hiv)-L-N-methylleucinyl(L- Me-Leu)-D-Hiv-L-N-methylvalinyl(L-Me-Val)-D-Hiv-L-Me-Val], a mixture of cyclo-[D-Hiv-L-Me-Leu-D-Hiv-L-N-methylisoleucinyl(L-Me-Ile)-D-Hiv- L-Me-Val] and cyclo(D-Hiv-L-Me-Ile-D-Hiv-L-Me-Leu-D-Hiv-L-Me-Val), and cyclo(D-Hiv-L-Me-Leu-D-Hiv-L-Me-Ile-D-Hiv-L-Me-Ile) by spectral analyses and chemical degradation. The IC50 values of enniatins D, E and F for ACAT activity in an enzyme assay using rat liver microsomes were calculated to be 87, 57 and 40 microM, respectively.

Animals↗
PubMed1992

Inhibition of acyl-CoA: cholesterol acyltransferase activity by cyclodepsipeptide antibiotics.

The effect was studied of the fungal cyclodepsipeptide antibiotics beauvericin and seven distinct enniatins on acyl-CoA: cholesterol acyltransferase (ACAT) activity. In an enzyme assay using rat liver microsomes, all the compounds were found to inhibit ACAT activity. The drug concentration that caused 50% inhibition (IC50 value) of the enzyme activity was determined to be 3.0 microM for beauvericin, indicating that the compound is one of the most potent ACAT inhibitors of microbial origin. Enniatins exhibited much higher IC50 values of 22 to 110 microM. More hydrophobic enniatins showed more potent inhibitory activity. Furthermore, the ACAT inhibitory activity was evaluated as inhibition of cholesteryl ester formation in a cell assay using J774 macrophages. Calculation of the ratio, CD50 value (the drug concentration causing 50% cell damage)/IC50 value of cholesteryl ester formation, indicated that beauvericin shows the highest specificity. These data indicate that beauvericin is one of the most potent and specific ACAT inhibitors of microbial origin.

Animals↗
PubMed1991

[Early diagnosis and prevention of hypoxic and ischemic encephalopathy and intracranial hemorrhage].

161 cases of newborn with history of perinatal hypoxic and clinical neural symptoms were intensively monitored by real-time ultrasound and clinical examination. Hypoxic and ischemic encephalopathy (HIE) and intracranial hemorrhage (ICH) were diagnosed in 44 cases. The incidences of HIE and ICH were 2.39 per 1,000 livebirths and 42.67 per 1000 cases of neonatal asphyxia or 12.7 per 1000 cases of fetal distress. The high risk factors contributing to HIE and ICH were investigated along with maternal complications during pregnancy and labor, neonatal complications and types of anoxia. The value of real-time ultrasound in the diagnosis of HIE and ICH was discussed. Early diagnosis and prediction of HIE and ICH were speculated upon.

Brain Ischemia↗
PubMed1991

Filariasis and its control in Fujian, China.

Epidemiological survey of filariasis in Fujian Province, China showed that malayan filariasis, transmitted by Anopheles lesteri anthropophagus was mainly distributed in the northwest part and bancroftian filariasis with Culex quinquefasciatus as vector, in middle and south coastal regions. Both species of filariae showed typical nocturnal periodicity. Involvement of the extremities was not uncommon in malayan filariasis. In contrast, hydrocele was often present in bancroftian filariasis, in which limb impairment did not appear so frequently as in the former. Hetrazan treatment was administered to the microfilaremia cases identified during blood examination surveys, which were integrated with indoor residual spraying of insecticides in endemic areas of malayan filariasis when the vector mosquito was discovered and with mass treatment with hetrazan medicated salt in endemic areas of bancroftian filariasis. At the same time the habitation condition was improved. These factors facilitated the decrease in incidence. As a result malayan and bancroftian filariasis were proclaimed to have reached the criterion of basic elimination in 1985 and 1987 respectively. Surveillance was pursued thereafter and no signs of resurgence appeared.

Animals↗
PubMed1990

Continuous sample collection in capillary zone electrophoresis by coupling the outlet of a capillary to a moving surface.

Use of an on-column frit structure, constructed by sintering a mixture of glass powders, makes it possible to ground a fused-silica capillary on its side prior to its outlet. Electroosmotic pressure permits convenient sample collection. We illustrate the use of this device by depositing the eluent in a continuous manner on a moving surface. This provides a permanent record of the separated species in a mixture.

Chromatography, Thin Layer↗