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Biomedical subjects

X Du

Publications and source records attributed to X Du.

175 records · Page 10Linked to original sources
PubMed1992

[Effect on anti-aging and treating yang deficiency of kidney with huanshao dan capsules. A clinical report of 309 cases].

In this study, with various dosages of huanshao dan capsules, an old Chinese medicine formula, the authors treated the patients with Yang-deficiency of kidney for 1.5-3 months and compared the effects of huanshao dan with those of watered pills and placebo. The analyses of Ridit's showed not only the scores of symptoms of the great-dose huanshao dan group (group 2), the great-dose watered pill group (group 3), the small-dose watered pill group (group 4) and but even the short-treating course group (group 5) were remarkably improved after treatment (P less than 0.001, P less than 0.01); but the group 6 have not any difference after treatment. However, the functions of liver and kidney, the level of cholesterol, the concentration of blood sugar, blood pressure and heart rate remained the same after treatment. The scores of the ability of prompt memory as well as serum lipid peroxide, serum adrenocortical hormone, cellular immunological function, index of glycoprotein and T3, T4 had similar changes. Our data suggested that both huanshao dan and watered pills were beneficial for anti-aging process and there was no noticeable difference between the two drugs.

Aged↗
PubMed1991

[Dynamic variation of saikosaponin contents].

In this paper both the cultivated and wild Bupleurum chinense and B. scorzonerifolium from northeast China of different growing periods and ages were analyzed for the contents of saikosaponins a and d by HPLC and total saikosaponins by spectrophotometry. The results indicated that the saikosaponin content of B. chinense was higher than that of B. scorzonerifolium, and that the saikosaponin contents of the cultivated species were higher than or almost equal to those of the wild ones. It was also found that both the cultivated and wild B. chinense and B. scorzonerifolium featured the highest contents of saikosaponins in the nutrition-growing period.

Anti-Inflammatory Agents, Non-Steroidal↗
PubMed1990

The reaction of singlet oxygen with proteins, with special reference to crystallins.

Photosensitized oxidation of the eye lens proteins, the crystallins, is thought to lead to protein crosslinks and high molecular weight aggregates. Such protein modifications may be important factors in the formation of lens opacities or cataracts. We focus attention here on type 2 photo-oxidation involving the reaction of singlet oxygen (1O2) with crystallins and some "control" proteins. We find that: (1) trp residues are oxidized to N-formyl kynurenine and related products, but this in itself does not lead to the production of high molecular weight protein aggregates of the protein; (2) tyr residues react with 1O2 but we do not detect dihydroxyphenylalanine or bityrosine nor are protein crosslinks formed as a result; (3) oxidation of his residues appears necessary for high molecular weight protein covalent aggregates to form. Proteins devoid of his, e.g. melittin or bovine pancreatic trypsin inhibitor, do not form high molecular weight products upon reaction with 1O2. Prior reaction and blocking of his inhibits the crosslinking reactions. (4) The oxidized protein is seen to be more acidic than the parent and has an altered tertiary structure. (5) Among the crystallins, reactivity towards 1O2 varies in the order gamma greater than beta greater than alpha and also gamma A/E greater than gamma D greater than gamma B crystallin.

Animals↗
PubMed1990

[Damage to cochleae in newborn guinea pigs by 80dB white noise].

Two groups of guinea pigs (the newborn and the adult) were exposed to 80dB SPL white noise for 72 hours. The effects were observed with E CochG, surface preparation, scanning electron microscopy and histochemical techniques. The results showed that: 1. the response threshold of the newborn was elevated after exposure; 2. the rate of OHC loss was higher in cochleas of the newborn than those of the adult; 3. OHC damage was mainly found in the fourth turn of cochlea of the newborn; 4. the LDH activity decreased in the newborn. It seems that the white noise could damage the hair cell function and structure in the newborn guinea pigs which are more susceptible to noise than the adult ones. The different susceptibility might be related to the degree of development and maturity of the cochleas.

Animals↗
PubMed1987

Glycoprotein Ib and glycoprotein IX are fully complexed in the intact platelet membrane.

Two new murine monoclonal antibodies, AK 1 and SZ 1, reactive with the human platelet glycoprotein (GP) Ib-IX complex have been produced by the hybridoma technique. Both AK 1 and SZ 1 immunoprecipitated the GP Ib-IX complex from Triton X-100-solubilized, periodate-labeled platelets. With trypsinized, labeled platelets, AK 1, SZ 1, and FMC 25 (epitope on GP IX) immunoprecipitated a membrane-bound proteolytic fragment of the GP Ib-IX complex consisting of GP IX and an congruent to 25,000 mol wt remnant of the alpha-chain of GP lb disulfide-linked to the beta-subunit. Unexpectedly, although AK 1 and SZ 1 immunoprecipitated purified GP Ib-IX complex, neither antibody immunoprecipitated the individual components of this complex, GP Ib or GP IX. When GP Ib and GP IX were recombined, however, AK 1 and SZ 1 again immunoprecipitated the reformed complex, strongly suggesting that both antibodies were recognizing an epitope present only on the intact complex. Cross-blocking studies indicated that AK 1 and SZ 1 recognized a very similar or identical epitope that was proximal to the epitope for FMC 25. Both AK 1 and SZ 1 bound to a similar number of binding sites (congruent to 25,000) on intact platelets as monoclonal antibodies directed against either GP lb or GP IX. The combined data suggests that GP lb and GP IX are fully complexed in the intact platelet membrane.

Antibodies, Monoclonal↗
PubMedDate not supplied

Analysis of Tlr4-mediated LPS signal transduction in macrophages by mutational modification of the receptor.

In mouse macrophages (RAW 264.7 cells), toll-like receptor 4 (Tlr4) is a limiting factor in lipopolysaccharide (LPS) signal transduction. The expression of only 1-2 x 10(4) copies of recombinant Tlr4 per cell enhances sensitivity to LPS, shifting the EC50 by 30-fold to the left. Expression of the Tlr4(Lps-d) isoform of Tlr4 (found in C3H/HeJ mice) shifts the EC50 2600-fold to the right, essentially abolishing LPS responses. A truncated form of Tlr4, lacking a cytoplasmic domain, exerts only a weak inhibitory effect on signal transduction. Similarly, the normal or Tlr4(Lps-d) forms of protein lacking an ectodomain [corrected], cause modest inhibition of LPS signaling. Manipulations of Tlr4 structure and expression cause changes in LPS sensitivity that range over 3 to 4 orders of magnitude. These findings support the view that Tlr4 is an integral component of a solitary pathway for LPS signal transduction in macrophages and permit inferences related to the mechanism of signaling and its blockade.

Animals↗
PubMedDate not supplied

Prevalence of Alzheimer's disease and other dementing disorders in an urban community of Beijing, China.

A door-to-door two-phase study was used to investigate the prevalence of senile dementia in an urban community of Beijing. In the study population, 5,003 individuals aged 60 years and older (>/=65 years, n = 3,728) were screened at home with the Chinese version of the MMSE. Persons who screened positive for dementia, using different cutoff scores based on degree of literacy, were further evaluated using the criteria of DSM-III-R and ICD-10. Among subjects who screened positive in phase I, 134 were diagnosed as having dementia in phase II. The prevalence ratios of dementia were 2.68% in the population aged 60 years and older, and 3.49% in the population aged 65 years and older. The prevalence rates among those aged 65 years and older were 1.85% for Alzheimer's disease, 1.37% for vascular dementia and 0.27% for other dementia (including mixed dementia). The prevalence of all dementia and AD increased steeply with advancing age and was consistently higher in women, but it was not obviously higher for VaD in women. Alzheimer's disease was the commonest type of dementia. Our prevalence figures for dementia and AD are similar to those previously reported for China.

Aged↗
PubMedDate not supplied

Interleukin-11: its biology and prospects for clinical use.

IL-11 is a multifunctional cytokine that has pleiotropic effects on a variety of tissue including the bone marrow and intestinal mucosa. IL-11 is useful in elevating the platelet count in cancer patients with chemotherapy-induced thrombocytopenia and has a cytoprotective effect on the bowel mucosa in a variety of causes of gut damage and a trophic effect on the villi after massive bowel resection.

Animals↗
PubMedDate not supplied

Phacofragmentation without perfluorocarbon liquid for dislocated crystalline lenses or lens fragments after phacoemulsification.

PURPOSE: To evaluate the clinical outcome of vitrectomy with phacofragmentation without perfluorocarbon liquid (PFCL) in the management of dislocation of the crystalline lens, caused by trauma, hereditary disorders, or hypermature cataracts, and lens fragments due to phacoemulsification. METHODS: A prospective study was conducted to evaluate 30 eyes of 29 patients who had undergone standard pars plana vitrectomy with phacofragmentation without PFCL in the vitreous cavity, for the removal of dislocated crystalline lenses or lens fragments, from January 1998 to July 2000. All cases were followed for more than six months. RESULTS: Final best corrected visual acuity of 0.5 or better increased from 0% preoperatively to 36.7% postoperatively, and 0.2 or better rose to 76.7%. The mean IOP was significant reduced, from 26.35 mmHg to 12.75 mmHg. No intraoperative complications occurred. Although two eyes (6.7%) developed retinal detachment, one (3.3%) had cystoid macular edema (CME) and one (3.3%) had a transient intraocular pressure increase (to 25 mmHg) postoperatively, all others had a favorable outcome. CONCLUSIONS: Standard pars plana vitrectomy with phacofragmentation without PFCL in the vitreous cavity is a safe, simple, and effective method for removing a dislocated crystalline lens or lens fragments, with good visual outcome.

Aphakia, Postcataract↗
PubMedDate not supplied

Measuring outcomes in care of the elderly.

This observational study was designed to assess whether routine measurement of outcomes using standard instruments is possible as part of clinical routine in care of the elderly, and to establish if such instruments are responsive to clinical change. Indices of functional status, cognitive function and subjective health status were collected routinely on admission and discharge in 540 inpatients and 340 patients attending a day hospital. Data collection became integrated into clinical routine. Response rates were generally good and yielded acceptably complete data. For inpatients, outcome was reflected by measurement of survival, physical function (Barthel index) and social status, each of these indicators showing significant change between admission and discharge. For day hospital patients, neither these, nor the Nottingham ADL scale, nor a health status indicator proved sufficiently responsive to clinical change to merit recommendation as outcome indicators for routine use in older patients. In ambulatory care in older patients, such as those attending a day hospital, new approaches are needed to measure clinical outcomes.

Activities of Daily Living↗