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Biomedical subjects

X Deng

Publications and source records attributed to X Deng.

At least 145 records · Page 8Linked to original sources
PubMed1997

The mouse Spam1 maps to proximal chromosome 6 and is a candidate for the sperm dysfunction in Rb(6.16)24Lub and Rb(6.15)1Ald heterozygotes.

We have determined the chromosomal localization of the murine gene encoding the 68-kDa sperm adhesion molecule 1, Spam1 or Ph-20. Using two independent approaches, fluorescence in situ hybridization (FISH) and interspecific backcross analysis we show the Spam1 maps to proximal mouse Chromosome (Chr) 6. This map position is within the conserved linkage group corresponding to human Chr 7q, where the human homolog, SPAM 1, has been shown to map previously. Genetic mapping shows the gene to be very closely linked to Met, one of the most proximal loci on MMU 6. It thus places the gene near the centromere and the junction of the Rb(6.16)24Lub and Rb(6.15)1Ald translocations. The essential role of the Spam1 sperm antigen in mouse sperm-egg interactions and its gene location provide strong support for its candidacy as the gene involved in the dysfunction of mouse sperm bearing the Rb(6.16)24Lub or Rb(6.15)1Ald translocation.

Animals↗
PubMed1997

The Dialine II graft: a new collagen-impregnated warp-knitted polyester arterial prosthesis.

The Dialine graft, a new prototype of knitted vascular prosthesis that uses a different brand of polyester fibers as an alternative to Dacron fibers, has been shown to offer excellent in vitro physical performance and in vivo healing. Although it still requires preclotting, the Dialine prosthesis was made impervious by impregnation of bovine type I collagen cross-linked with vapors of formalin. The purpose of the present investigation was to compare the in vitro physical characteristics of the Dialine II graft with those of the collagen-impregnated Hemashield graft. In addition, we studied the healing performance as a thoracoabdominal bypass in dogs for prescheduled periods of implantation ranging from 4 hours to 6 months. In vitro, the bursting strength, resistance to dilatation, and suture retention strength properties of the Dialine II prosthesis were all shown to exceed those of the Hemashield control graft. In the first weeks after implantation, the Dialine II grafts induced a discrete inflammatory response, as shown by the constant leukocyte counts observed both before implantation and when the animals were killed, as well as by the histologic observation of a few inflammatory cells in contact with the collagen. Consequently, the Dialine II grafts showed a slow rate of bioresorption of cross-linked collagen. At 1 month, a thin internal collagenous capsule was present at both anastomoses, laying over the original collagen coating. At 3 and 6 months, areas of thrombotic deposits and endothelialized areas were observed on the luminal surface. Because results of early clinical trials have been highly satisfactory, this prosthesis may be recommended for use without restriction as a medium- and large-diameter blood conduit.

Animals↗
PubMed1997

Are intraaortic balloons suitable for reuse? A survey study of 112 used intraaortic balloons.

To assess the safety of reusing single-use intraaortic balloon devices (IABs), 112 used devices were investigated in terms of physical integrity, gas leakage inspection, mechanical performance, surface chemistry and morphology, and physical stability. These IABs were all used clinically only once, and the duration of the IABs in vivo ranged from 6 to 312 h. Macroscopic examination of the balloons and the outer catheters revealed no obvious change in either shape or color. No discernible abrasions or cracks were observed on the balloons. However, 61% of the balloons were creased, and 40% of the central lumens and 21% of the sheaths showed visible bending flaws. Moreover, 65% of the balloons and 38% of the central lumens were contaminated by visible residual organic debris. The physical integrity of each device was verified in a specially designed leakage-fatigue tester for 72 h. Ninety-seven percent of the devices passed the leakage inspection. Stress-strain testing, differential scanning calorimetry, attenuated total reflection-Fourier transform infrared, and scanning electron microscopy analyses clearly indicated that there were no significant differences in the mechanical properties, bulk material morphology, surface chemistry, and external surface morphology between the used balloons and virgin controls. Although some surface modifications occurred on the internal side of the balloons, the external surfaces of most balloons suffered no trauma. Most of the used IABs examined in this study maintained physical and mechanical properties similar to those of the virgin devices. The chemistry of the balloon material was stable after short-term in vivo use. However, it does not seem possible to establish a rigorous protocol of cleaning, sterilization, and inspection to guarantee a safer reuse of these devices. The presence of residual organic debris that cannot be eliminated results in an imperative preclusion not to reuse the IABs.

Calibration↗
PubMed1997

[Role of endothelial-derived nitric oxide and its synthase in the development of hypoxic pulmonary hypertension in rat].

To clarify the role of endothelial-derived nitric oxide (EDNO) and its synthase (NOS) in the normal and hypertensive pulmonary vasculature, activity of endothelial NOS in the lungs, ENDO-dependent vasodilating response induced by bradykinin (BK), and cGMP content of lung tissue in normoxic and hypoxic rats were investigated. We also studied the effects of NOS inhibitor-L-NAME on the activity of NOS, cGMP content, mean pulmonary arterial pressure (mPAP) and carotid systolic arterial pressure (CAPs) in both rats. The results were as follows (1) In normoxic rats there was no NOS activity in the endothelium of small vessels (phi < or = 80 microns) and no relaxing response to BK. Long-term administration of L-NAME obviously inhibited the activity of ecNOS and cGMP content in the lungs of normoxic rats, therefore it led to the increment of CAPs but failed to elevate mPAP. (2) After hypoxic exposure for 10 days, NADPH-diaphorase (NADPH-d and ecNOS immunoreactivity turned to be positive in the endothelium of small vessels with diameter less than 80 microns. BK-induced EDNO-dependent vasodilation, the enzyme activity of cNOS and cGMP content in the lungs of hypoxic rats were significantly enhanced as compared with normoxic rats. Long-term administration of L-NAME in hypoxic rats markedly inhibited the enhancement of cNOS enzyme activity, the production of EDNO and cGMP content in rat lungs, consequently it significantly decreased mPAP but elevated CAPs obviously. These results suggest that the role of EDNO in maintaining the low basal tone of normal adult pulmonary circulation remain to be studied more precisely. The increased activity of ecNOS and the enhancement of EDNO synthesis might act to moderate the hypertension. The excess synthesis of EDNO might be toxic to the endothelium of pulmonary vessels, therefore potentiating the development of pulmonary hypertension.

Animals↗
PubMed1997

Arterial wall mechanical characteristics after treatment in collagenase: an in vitro aneurysm model.

OBJECTIVE: To investigate the mechanical characteristics of canine aortas treated with buffered collagenase as a first step in developing an animal model of aortic aneurysm for the validation of stent-grafts. DESIGN: In vitro study of canine aortas. INTERVENTIONS: Canine thoraco-abdominal arteries were divided into the descending thoracic aorta the suprarenal artery and the infrarenal artery; these segments were incubated separately in a buffered collagenase solution for 1 to 6 hours. Some segments were left untreated as controls. OUTCOME MEASURES: Mean arterial wall thickness, measured with Vernier callipers and computerized histomorphometric methods and longitudinal tensile strength of control and treated vessel segments. RESULTS: The arterial wall thickness decreased with incubation time. After 1 hour of incubation the reduction was approximately 15% for the descending thoracic aorta, 16% for the suprarenal artery and 18% for the infrarenal artery. After 6 hours the total reduction in wall thickness was 32%, 41% and 44% respectively for the 3 segments. The tensile strength of the treated arterial segments also decreased with the incubation period. Initially, the infrarenal segment displayed the greatest strength; however, this was reversed as the period of incubation increased. The inelastic limit of the descending thoracic aorta control segment was reached at 100% elongation, whereas that of the suprarenal artery was reached at 80% and that of the infrarenal artery was reached at 60% elongation. All of the arterial segments became weaker as the period of incubation in buffered collagenase increased. CONCLUSION: This in vitro incubation technique successfully altered the structure of the collagen fibre network within the arterial wall. This method may be an option in developing an aneurysm model to test stent-grafts in vivo.

Aneurysm↗
PubMed1997

Highly efficient induction of sugar beet plant regeneration.

A method for high-frequency in vitro regeneration of adventitious shoots from petiole explants of a tetraploid breeding line 405 of Beta vulgaris L. was established. Plant regeneration was obtained by direct bud formation from petioles. Explants were precultured in a medium containing MS inorganic salts supplemented with Gamborg's vitamins, named MSB. Adventitious shoots were developed when petiole explants excised from 20-30-day-old seedlings grown on MSB containing 0.3 mg/L naphthalene acetic acid (NAA), 1.0 mg/L 6-benzylaminopurine (BA), and were then cultured on MS with 0.3 mg/L NAA and 1.0 mg/L BA. Shoots rooted on half-strength MS medium containing 0.5-1.0 mg/L NAA. This procedure of the culture method may be very useful both for multiplication of sugar beet and for the introduction of foreign genes into Beta vulgaris. The proliferation rate of induced plantlets is as high as 51.3%.

Chenopodiaceae↗
PubMed1997

[Localization and expression of dopamine receptors in stomach and duodenum in rats].

UNLABELLED: Dopamine is an important neurotransmitter which may contribute to the regulation of gastrointestinal function, but its mechanism remains elusive. OBJECTIVE: The existence, localization and expression of dopamine receptor mRNAs in rat stomach and duodenum were examined by in situ hybridization. METHODS: The oligonucleotide probes were selected and synthesized according to the previous work. All the probes were labelled with alpha S35dATP using the terminal deoxynucleotide transferase, then were hybridized with the frozen, fixed preparation of rat stomach and duodenum. The density of silver granules were counted and analyzed by the image analysis computer system. RESULTS: It was found that all five subtypes of dopamine receptor mRNAs existed in the stomach and duodenum with greatly different expression. The distribution of dopamine mRNAs in the stomach were mainly localized in the lamina propria near the lamina muscularis mucosae and parallel to the longitudinal axis of the tract. Whereas, all five MRNAs were spread over the entire layer of the duodenal mucosa. With the image analysis system, abundant expressing levels of D5 and D4 mRNAs were documented unexpectedly. CONCLUSION: All these results suggest that dopamine produced its effect via dopamine receptors especially D5 subtype and D4 receptor might be act as a modulator.

Animals↗
PubMed1997

Fusion expression of green fluorescent protein and HCV capsid antigene in Escherichia coli cells.

A chimeric gene of the green fluorescent protein (GFP) and hepatitis C virus (HCV) core antigene were constructed and expressed in E. coli cells. The expressed fusion protein was examined by Dot-ELISA and Western blot and the three antigenic determinants were detected. The GFP-Core fusion protein showed not only the striking green fluorescence under natural light but also the HCV antigenic activity. A new method of immunological diagnosis is greatly anticipated in the light of this fusion protein which can be seen as the HCV antigen tagged with the green fluorescent protein.

Animals↗
PubMed1997

[The relations between mutations of p53 anti-oncogene in atherosclerosis and levels of lipids in serum].

OBJECTIVE: To further understanding the pathogenesis of human atherosclerosis, we studied the mutations of p53 anti-oncogene in Chinese atherosclerosis lesions and the relations between p53 gene mutations with the levels of serum lipoprotein and the contents of lipids in AS tissues. METHODS: p53 gene mutations were detected by a-32P-dCTP labelled radiative PCR-SSCP and the biochemical analyses of lipids in serum and AS tissues. RESULTS: In 89 cases studied, 9 were found the p53 gene multi-hotsports exons mutations. The TC, TG and CE contents in vascular AS tissues in p53 gene mutation group were higher than those in non-mutations group (ANOV: F = 4.56-9.97, P < 0.05-0.01, no differences in PL contents). But there were no statistic differences in serum levels of TC, TG, CE and apoAI between the two groups (P > 0.05-0.25). All the levels of the serum lipoproteins studied were almost in normal ranges. CONCLUSION: The DNA structural mutations of p53 anti-oncogene in Chinese adults' AS tissues have remarkable relations with the severity of AS lesions. The mutations of p53 gene may play some important roles in the formation of human atherosclerosis. In addition to serum lipid disorders, this may be a new mechanism in the pathogenesis of human atherosclerosis.

Adult↗
PubMed1997

[No point mutation of the 2.8 kb EcORI fragment of the nasopharyngeal carcinoma transforming gene TX in nasopharyngeal carcinoma].

We have previously cloned a nasopharyngeal carcinoma transforming gene from human nasopharyngeal carcinoma cell line CNE-2 using mouse JB6 cell line as the recipient cells. This transforming gene, designated Tx, exhibits moderate transforming activity upon transfection into JB6 cells. Several lines of evidence indicate that the 2.8 kb EcoRI fragment which lies in the middle of the Tx gene is responsible for the transforming activity. The sequence of the 2.8 kb EcoRI fragment was determined, which showed striking homology with the human immunoglobulin light chain C region gene. Using the modified polymerase chain reaction-single strand conformation polymorphism analysis (PCR-SSCP) silver staining technique, we studied the possible point mutations of the nasopharyngeal carcinoma gene Tx in nasopharyngeal carcinoma. No mobility shift was detected in eleven paired cases of nasopharyngeal carcinoma biopsies suggesting that in most cases of nasopharyngeal carcinoma, the Tx gene is not activated via a mode of point mutation. Taken together with the previously obtained results we have found that the Tx gene is not activated by gene rearrangement or gene amplification. Therefore, we consider that the Tx gene may have been activated by its interaction with other regulatory factors.

Carcinoma, Squamous Cell↗
PubMed1997

[Evaluation of optimal measuring site and index by QDR4500A for postmenopausal bone loss].

As the function of most bone mineral measuring machine is limited, either by single photon absorptiometry (SPA), dual photon absorptiometry (DPA), speed of sound (SOS), or dual energy x-ray absorptiometry (DEXA), it is hard to evaluate the best place and index of postmenopausal bone loss at many positions by one machine at the same time. So we used QDR4500A, one of the best advanced DEXA which has a good repeatability, to search the best place and index for postmenopausal bone loss. The results showed that (1) bone mineral density (BMD) was better than bone mineral content (BMC) and Area to reflect this kind of bone loss; (2) lumber BMD was a better place than the forearm BMD, total spine BMD and other places; and (3) midlateral L3, 4 BMD was a best index for postmenopausal bone loss.

Absorptiometry, Photon↗
PubMed1996

Alterations in endothelial barrier permeability in multiple organs during overactivation of macrophages in rats.

Macrophage hyperactivity has been suggested to play an important role in septic complications and the development of multiple organ failure. Intraperitoneal administration of macrophage stimulants, e.g., zymosan, induce a systemic inflammatory response, with concomitant gut origin sepsis, and organ dysfunction. However, little is known about alterations in endothelial permeability during macrophage hyperactivation. In the present study, the effect of macrophage hyperactivation on endothelial permeability, assessed by 125I-labeled HSA and 51Cr-labeled EDTA, and the difference between cytolytic and noncytolytic inflammatory macrophages induced by i.p. injection of .25 or .50 mg/g of zymosan, concanavalin A (Con A) or thioglycollate medium (TM) diluted in 4 mL of paraffin, as well as the potential relationship with the doses used, were evaluated in the rat. Overactivation of cytolytic inflammatory macrophages induced a pronounced alteration in endothelial barrier permeability, characterized by a decrease in whole body plasma volume and an increase in whole body interstitial fluid volume, while overactivation of noncytolytic inflammatory macrophages only induced leakage of proteins and plasma to several of the organs studied. Macrophage activators, like zymosan, Con A and TM, exhibited varying effects on endothelial permeability related to the dose used. The results in the present study imply that overactivation of cytolytic inflammatory macrophages may play an important role in endothelial barrier injury and that zymosan possesses a more potent effect as compared to Con A when administered at the same dose.

Animals↗
PubMed1996

Efficiency of an external support to reduce lipid infiltration into venous grafts: in vitro evaluation.

Excessive distension of venous grafts due to arterial pressure enhances the convective water transport (filtration flow) through the vessel wall, and thus might affect the infiltration of macromolecules such as lipoproteins. In this paired experimental study, filtration velocities were measured at 100 mm Hg for canine jugular veins with or without external supports of expanded polytetrafluoroethylene (ePTFE) arterial prostheses. In addition, to assess the effect of filtration velocity on lipid infiltration or uptake, canine jugular veins were wrapped over half of their lengths with ePTFE arterial prostheses and perfused with dog serum containing 3H-cholesterol at a pressure of 100 mm Hg. At 100 mm Hg, the average filtration velocity of the wrapped jugular veins was 7.9 +/- 1.3 x 10(-6) cm/s whereas the average filtration velocity of the unwrapped veins was 27.3 +/- 2.7 x 10(-6) cm/s (p < 0.005). Moreover, the unwrapped veins had a significantly higher uptake rate of labeled cholesterol than the wrapped veins (10.9 +/- 7.3 x 10(-4) cm/h and 5.0 +/- 1.6 x 10(-4) cm/h, respectively, p < 0.005). In conclusion, under arterial pressure, veins experience excessive distention, which leads to significant increases in both filtration flow and cholesterol uptake. An external wrap or support of ePTFE material protects veins from excessive distension and thus may prevent atherosclerosis in venous grafts by reducing cholesterol uptake.

Animals↗
PubMed1996

Role of nitric oxide in short-term and prolonged effects of angiotensin II on renal hemodynamics.

Short-term infusions of angiotensin II (Ang II) increase renal vascular resistance and thereby endothelial shear stress and nitric oxide (NO) release. Prolonged stimulation of Ang II can decrease the expression of NO synthase isoforms in the macula densa, but prolonged increases in shear stress can increase transcription of endothelial NO synthase. Therefore, we designed these studies to test the hypothesis that Ang II exerts time-dependent effects on renal NO generation as assessed from renal excretion of nitrate and nitrite, percent increases in renal vascular resistance during inhibition of NO synthase with intravenous NG -nitro-L-arginine methyl ester (L-NAME), or decreases in renal vascular resistance during stimulation of endothelial NO synthase with intravenous acetylcholine. Rats were tested during graded short-term (30 to 90 minutes intravenous) or prolonged (5 to 6 days subcutaneous) Ang II infusions that led to dose-dependent increases in blood pressure and renal vascular resistance and reductions in renal blood flow. Captopril was administered for 3 to 4 days to suppress Ang II generation. The renal excretion of nitrate and nitrite was increased during short-term Ang II infusions (from 205 +/- 22 to 331 +/- 58 pmol.min-1, P < .05) but was unchanged during prolonged Ang II infusion (control group, 197 +/- 33 versus Ang II, 245 +/- 42 pmol.min-1, P=NS). The percent increase in renal vascular resistance with L-NAME was potentiated dose dependently by short-term but not long-term Ang II infusions. The increase in renal vascular resistance with L-NAME in control rats without Ang II infusions was +150 +/- 13%. At an Ang II infusion of 200 ng.kg-1.min-1, the L-NAME-induced percent increase in renal vascular resistance was significantly (P < .01) increased compared with controls in short-term Ang II-infused rats (+369 +/- 70%) but was not significantly different in prolonged infused rats (+190 +/- 33%). Intravenous acetylcholine caused dose-dependent renal vasodilation that was not significantly changed in rats receiving short-term intravenous Ang II but was significantly (P < .005) potentiated in those receiving prolonged Ang II infusions (change in renal vascular resistance with acetylcholine at 10 micrograms.kg-1.min-1 versus control, -21.5 +/- 5.0%; with short-term Ang II, -24.9 +/- 4.5%; with long-term Ang II, -52.1 +/- 7.2%). In conclusion, short- and long-term Ang II infusions caused equivalent changes in blood pressure and renal blood flow and hence presumably equivalent increases in endothelial shear stress. However, only short-term Ang II infusions increased NO generation and the dependence of the renal circulation on NO, whereas acetylcholine-induced NO release was enhanced selectively during long-term Ang II infusions. This suggests that during long-term Ang II, renal NO release may become uncoupled from shear stress yet remains highly responsive to receptor-mediated stimulation.

Acetylcholine↗
PubMed1996

Gross, histological, and microvascular anatomy and biomechanical testing of the spring ligament complex.

In recent years there has been an increased interest in the treatment of acquired pes planus. The breakdown of the medial longitudinal arch is most often seen at the talonaviculocalcaneal articulation. This suggests a relationship between the ligamentous complex at this articulation and acquired pes planus. This study was undertaken to gain a better understanding of the gross, histologic, and microvascular anatomy, as well as the biomechanics of the ligamentous structures surrounding the talonaviculocalcaneal articulation. Cadaver dissections of 38 fresh-frozen feet were performed. Detailed descriptions of the gross anatomy of the superomedial calcaneonavicular ligament, inferior calcaneonavicular ligament, and the superficial deltoid ligament were recorded. Their relationships to the posterior tibialis tendon and to the bones of the talonaviculocalcaneal articulation are described. The histology and microvascularity of these structures were also studied. Preliminary biomechanical testing was performed. It was found there are two definitive anatomic structures that are commonly called the spring ligament: the superomedial calcaneonavicular ligament (SMCN) and the inferior calcaneonavicular ligament (ICN). The SMCN ligament was found to have histologic properties that suggest significant load bearing. The histology of the ICN ligament suggests a pure tensile load function. The deltoid ligament and the posterior tibialis tendon had direct attachments to the SMCN ligament in all specimens. An articular facet composed of fibrocartilage was found in each SMCN ligament specimen. The microvascular structures showed an avascular articular facet present in the ligament. The biomechanical testing showed that the SMCN ligament and ICN ligament had strength similar to ankle ligaments. This study suggests this "spring ligament complex" has more of a "sling" function for the talar head. It is hoped that the better understanding of this region will add to our understanding of the etiology of pes planus and possible treatment alternatives.

Biomechanical Phenomena↗
PubMed1996

Theoretical modelling of the release rate of low-density lipoproteins and their breakdown products at arterial stenoses.

Arterial stenoses and luminal-surface irregularities at anastomoses cause blood-flow disturbances with slow recirculation. The authors created a computer simulation to study the rates of the release into blood of atherogenic substances such as low-density lipoproteins and their breakdown products from within the arterial walls at stenoses. Finite-difference methods were used to solve the Navier-Stokes equations (in the form of stream function and vorticity function) and the steady-state mass transfer equation for bell-shaped stenoses with two different degrees of constriction. This simulation indicated that the efflux rates of lipids and their breakdown products from the vessel walls were suppressed in the region of disturbed flow, with slow circulation distal to stenoses. The lowest efflux rate was found at the point of flow separation, and this rate was much lower than rates in regions of undisturbed flow. Therefore, this mathematical model predicts that locally disturbed blood flow at arterial stenoses and arterial anastomoses is responsible for two distinct phenomena: first, it provides favourable conditions for lipid infiltration into vessel walls; and, second, it impairs the release into the blood of atherogenic substances accumulated in the vessel wall. Such mass transfer abnormalities may account for atherogenesis and the late failures of arterial reconstructions at these sites.

Animals↗