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Biomedical subjects

X Chen

Publications and source records attributed to X Chen.

At least 991 records · Page 55Linked to original sources
PubMed1998

[Clinical study on effect of Bushen Tiaogan Lianru recipe on hyperprolactinemia].

OBJECTIVE: To observe the therapeutic effect of Bushen Tiaogan Lianru Recipe (BTLR) on patients with hyperprolactinemia (HPRL). METHODS: Patients were divided into three groups: 38 cases in the BTLR group, 22 in the Bromocriptine group and 12 in the control group. RESULTS: BTLR could reduce the serum prolactin level of patient markedly. It had a good regulatory action on the relevant hormones with an ideal clinical therapeutic effect, which was similar to the effect of bromocriptine. No effect had revealed in the control group treated with placebo. CONCLUSION: BTLR is effective in the treatment for HPRL and has a good prospect of development and application.

Adult↗
PubMed1998

[Effect of Tripterine on mRNA expression of c-myc and platelet derived growth factor of vascular smooth muscle cell in rats].

OBJECTIVE: To observe the effect of Tripterine on mRNA expression of c-myc and platelet derived growth factor (PDGF) in vascular smooth muscle cell (VSMC) of rats. METHODS: 5-10th passage culture of VSMC was used. Tripterine and 20% fetal calf serum were added into the medium of cultured VSMC in concentration of 0.1, 0.2 and 0.3 mg/L after free-serum cultivation for 24 hours. The general RNA was isolated from VSMC at 6 and 12 hours after the drug addition for detection of mRNA expression of c-myc and PDGF respectively by dot blot hybridization. The cDNA probes were labeled by digoxin. RESULTS: Tripterine inhibited the PDGF mRNA expression of VSMC and decreased c-myc mRNA expression in a dose-dependent manner, either vs. control. CONCLUSION: Tripterine could inhibit mRNA expression of c-myc and PDGF in VSMC, therefore, it would inhibit overproliferation of VSMC.

Animals↗
PubMed1998

[Effect of hypoxia on proliferation and alpha 1 (I) procollagen gene expression by human fetal lung fibroblasts].

OBJECTIVE: The effect of 2% hypoxia on the proliferation and alpha 1(I) procollagen gene expression by human fetal lung fibroblast was studied. METHODS: Cell proliferation was determined by cell counting and nonradioactive cell proliferation assay method. alpha 1(I) procollagen gene expression was detected by Northern blot hybridization analysis. RESULTS: Cell number and 490 nm OD expressing low oxygen values were markedly increased by the 24, 48 and 72 h exposure to 2% hypoxia respectively. An obvious increase of the alpha 1(I) procollagen mRNA expression was detected after 24 h exposure to 2% hypoxia. The increase of gene expression could be inhibited by Ligustrazin (30 micrograms/ml, 10 micrograms/ml). CONCLUSIONS: 2% hypoxia plays an important role in the outcome of wound healing and pulmonary vascular remodeling by affecting the function of human fetal lung fibroblasts.

Cell Division↗
PubMed1998

[Salvia Miltiorrhizae Composita in preventing PaO2 lowering induced by controlled hypotension with nitroglycerin].

OBJECTIVE: To observe the effects of Salvia Miltiorrhizae Composita (SMC) on blood gas variations of nitroglycerin (NTG) controlled hypotension. METHODS: Sixteen patients who were arranged to undergo operation under general anesthesia in controlled hypotension condition were divided randomly into control group (n = 8) and SMC group (treated group, n = 8), NTG were used to create controlled hypotension in both groups and blood pressure decrease about 30% comparing with the control values. Patients in the treated group were administered intravenously with SMC (16 ml) before the utilization of NTG. RESULTS: Significant PaO2 decrease and Pa-etCO2 increase were observed in the control group during the controlled hypotension period. However, blood gas values in the treated group did not evidently change. CONCLUSION: It suggested that SMC can prevent the side effects of NTG on blood gas.

Adult↗
PubMed1998

[Application of capillary electrophoresis in speciation analysis].

Each species has different chemical and biological behaviors. Toxicity is often dependent on it. So element speciation is important in a variety of environmental, biological, geological, and medical applications. Recently, capillary electrophoresis(CE) has been applied to speciation analysis owing to its high efficiency and short analysis time. This paper reviews the applications of CE-UV and CE-ICP-MS in speciation analysis and discusses some related problems.

Arsenic↗
PubMed1998

[A study on the nutrient physiology of Ophiopogon japonicus (Thunb.) Ker-Gawl].

By means of observing at regular intervals and on tixed plants, the growth stages, organogenetic habits, content of nutrient elements and its changing regularity, storage habit of dry matter, relationship between aerial and subterraneous parts, and fertilizer requirements have been studied for Ophiopogen japonicus.

Drugs, Chinese Herbal↗
PubMed1998

[Influence of processing methods on alkaloid, toxicity and effect of Strychnos nux-vomica L].

Determination has been made on the contents of strychnine, brucine and ephedrin in different processed products of Strychnos nux-vomica. The acute toxicity, analgesic and antiphlogistic actions of these products have also been detected. The result shows that the product processed with Ephedra sinica can reduce toxicity and promote curative effect. Among the different processing methods the preparation with Ephedra and Liqorice root and the preparation with Ephedra and alcohol appear better and thus useful in practical application.

Animals↗
PubMed1998

[Experimental study of dahuang zhechong pills in the treatment of immunological liver fibrosis in rats].

OBJECTIVE: To study the effect of DHZC on liver fibrosis. METHOD: Immuno-injured fibrosis in rats was induced by bovine serum albumin and DHZC was given during and after the model production respectively. Hepatic collagen contents, pathology of liver and serum hyaluronic acid were measured. Colchicine was used as control. RESULT: It shows that the liver fibrosis rates of the DHZC treated group during and after the model production were 72.7% and 71.2% respectively, while those of the untreated and colchicine group were 92.3%-100%. Compared with the untreated group, there was a remarkable decrease of hepatic collagen contents in the DHZC-treated rats. CONCLUSION: DHZC have thus been proved helpful in reversing liver fibrosis to some extent.

Animals↗
PubMed1998

[Production, identification and redirected cytotoxicity of CD3/CD19 bispecific monoclonal antibody].

OBJECTIVE: To prepare CD3/CD19 bispecific monoclonal antibody (BsAb) and study its biological effects. METHODS: The CD3/CD19 BsAb was prepared by using hybri-hybridoma technique. The presence of BsAb was demonstrated by double-isotype ELISA and cell conjugate assay. RESULTS: We obtained three strains of CD3/CD19 quadromas, including a stable one. 51Cr release assay showed that CD3/CD19 supernatants, CD3/CD19 purified antibody and CD3/CD19 chemical heterocongjugate greatly enhanced the cytotoxic activity of CD3 AK cells against Nalm-6 cells (P < 0.05-0.001). CONCLUSIONS: The CD3/CD19 BsAb we prepared was efficient in redirecting activated T cells against target tumor cells. This indicates that the CD3/CD19 BsAb has potential value of clinical use.

Animals↗
PubMed1998

[Clinical study and denominate of traumatic herniation of corpus adiposum buccae].

OBJECTIVE: To deepen the recognition, and provide the evidence for rational name of traumatic herniation of corpus adiposum buccae. METHODS: 10 clinical cases of traumatic herniation of corpus adiposum buccae were studied. Simulat experiment on 6 fresh corpses (12 sides) were performed dissected immediately after bump (hitting) type and stab (thrusting) type of herniation of corpus adiposum buccae. RESULTS: In one side bump type and 5 sides stab type successful experiments, the buccal protuberance of masticatory fat pads (namely corpus adiposum beccae) were herniated into the mouth, with the shape, size, and position simulated with the herniated tissue of the clinical case. CONCLUSION: The herniated tissue of clinical case is the corpus adiposum buccae but not the buccal fat pad, and the rational name should be traumatic herniation of corpus adiposum buccae.

Cheek↗
PubMed1998

[Evaluation in the relationship of disc displacement of temporomandibular joint to osteoarthrosis in the rabbit].

OBJECTIVE: The purpose of this study, using an animal model, was to evaluate the relationship between disc displacement of temporomandibular joint (TMJ) and osteoarthrosis. METHODS: The disc was surgically displaced anteriorly in the right TMJ in each of 25 rabbits. The TMJs were removed after 1, 2, 4, 8, and 12 weeks and stained with H&E. RESULTS: 11 joints had partial anterior disc displacement, 8 joints with complete anterior disc displacement and 6 joints with perforation. The partial anterior disc displacement joints showed early degenerative or physiologic remodeling changes, the complete anterior disc displacement and perforation joints showed degenerative or severe osteoarthritic changes. CONCLUSION: The degree of anterior disc displacement of TMJ relates to the osteoarthrosis. The farther anteriorly, the more easily disc displacement can cause the osteoarthrosis.

Animals↗
PubMed1998

[Cervical spondylotic myelopathy: early diagnosis and timing of operation].

OBJECTIVES: To study early clinical characteristics and diagnosis of cervical spondylotic myelopathy and to search the timing of surgery. METHOD: Seventy-four cases of early diagnosed cervical spondylotic myelopathy (CSM) were treated with anterior cervical decompression and autologous ilium graft fusion. RESULT: The early stage of CSM was indistinct, and symptoms were mild. Neck pain, abnormality of motion and sensation of limbs, and hand and arm symptoms were shown frequently. Changes in neurological function could found during physical examination. Characteristic changes in imaging were helpful in early diagnosis of the disease. CONCLUSION: Early diagnosis and surgery of CSM are important in improving the therapeutic results.

Adult↗
PubMed1998

[Clinicopathologic factors influencing the serum of interleukin-12 level of patients with PHC: multivariate statistical analysis].

OBJECTIVE: To investigate clinicopathologic factors influencing the serum of IL-12 level of patients with PHC. METHOD: Serum interleukin-12 (IL-12) level was determined by ELISA in 16 patients with primary hepatic carcinoma (PHC). The following clinicopathologic factors were considered for the serum IL-12 effect: age, serum albumin and serum total bilirubin, size of tumor, number of tumor, tumor thrombus of portal vessels (present or absent), time of operation, tumor resected (yes or no), histological grade of tumor, and the amount of lost blood during operation. Dependent variables were serum IL-12 level at 1 day before operation and at 1 and 7 days after operation. The multivariate results were confirmed using multiple regression analysis. RESULT: Size of tumor and serum albumin greatly affected serum IL-12 level before operation. Serum total bilirubin and age affected serum IL-12 level. It was significantly different before and after operation, because of the amount of lost blood. CONCLUSION: The main effective factors of serum IL-12 level were size of tumor and the hepatic function of patients. Operative trauma was the major factor reducing IL-12 level after operation.

Adult↗
PubMed1998

[Cellular apoptosis, proliferation and bcl-2 Bax expression in colorectal carcinoma and their association with tumor prognosis].

OBJECTIVE: To investigate the relationship between cellular apoptosis, proliferation and bcl-2/bax expression in the tissue of colorectal carcinoma and biological behavior of the tumor. METHOD: The apoptotic cells index (AI) in situ were identified by the terminal deoxynucleotidyl transfer-mediated dUTP-biotin nick end labeling (TUNEL) and immunohistologic staining for Ki-67 proliferation index (KI), bcl-2/bax protein expression was performed on archival material from 77 adenocarcinomas. RESULT: The mean AI and KI were 5.3/45.4 in early stage cancer, 5.6/48.7 in non-metastatic cancer, 6.6/55.3 in advanced cancer and 8.9/60.1 in cancer with distant metastasis respectively. There was a positive relationship between the AI and KI in each specimen. bcl-2 and bax was expressed as 54.5%, 74% in carcinoma and highly differentiated carcinoma. In the univariate analysis, significantly longer survival time was observed in the subgroup of bcl-2 positive carcinoma with low AI. In the multivariate analysis, tumor stage, tumor type, KI, and bcl-2 expression were independent risk factors for prognosis. CONCLUSION: The data indicate that abnormally exchanged AI/KI, bcl-2/bax expression appears to be an event associated with tumor progression and apoptosis might also reflect the proliferative activity of human colorectal carcinoma.

Apoptosis↗
PubMed1998

[Clinical significance of early diagnosis of cervical spondylotic myelopathy].

OBJECTIVE: To probe into the possibility of early diagnosis of cervical spondylotic myelopathy (CSM) prospectively and to screen the premorbid signs of CSM clinically, radiologically and electro-physiologically. METHOD: Twenty-nine items related to the occurring of CSM were chosen as common characteristics of CSM and considered as the criteria of prospective study. 81 patients who met the criteria were studied. All patients were followed up and observed for 2 - 6.5 years (mean 3.7 years). RESULT: Twenty-nine patients showed CSM during investigation. Thirteen items of the criteria were related to the occurring of CSM. The significant items include upper limb pain and abnormal sensation limb numbness, positive dynamic Hoffmann's sign, cervical canal stenosis, lower cervical instability, cervical intervertebral disc herniation, and delay of central motor conduct time. CONCLUSION: CSM can be early diagnosed. The patients who meet the established criteria should be followed up and observed closely. Early operation results in good effect.

Adult↗
PubMed1998

[Six year follow-up of suspects of primary angle-closure glaucoma].

OBJECTIVE: To understand the natural history and the risk factors of primary angleclosure glaucoma and obtain experiences that might help screening for the disease. METHODS: Four hundred and eighty-five suspects were screened from 6 548 population aged over 40 years. The suspects were then followed up within six years. The following examinations were performed: visual acuity, axial anterior chamber depth, peripheral anterior chamber depth, intraocular pressure and cup/disc ratio of optic nerve papilla. The suspects with high risk were further examined. RESULTS: During the follow-up period, twenty patients (4.1%) in the suspects developed angle-closure glaucoma, 14 cases had natural attack (Acute onset developed in 6 cases and 8 cases were in chronic stage) and 6 cases were found at early stage. The anterior chamber depth shallowed progressively in 28% of suspects. CONCLUSION: Anterior chamber depth is an important index for screening of primary angle-closure glaucoma, and the regular follow-up is of significance for the suspects.

Anterior Chamber↗
PubMed1998

[Observation of anterior chamber angle with ultrasonic biomicroscopy after posterior chamber implantation of intraocular lens].

OBJECTIVE: To observe the changes of anterior chamber angle after extracapsular cataract extraction (ECCE) with posterior chamber implantation of intraocular lens (IOL) and discuss the associated factors. METHODS: The changes of anterior chamber angle in 31 cases (41 eyes) with senile cataract were observed with ultrasonic biomicroscopy after ECCE and posterior chamber implantation of IOL. RESULTS: (1) There were 6 eyes with peripheral anterior synechia of the iris, 12 eyes with abnormal pupillary configuration, which were related to the position of IOL haptics. (2) The IOL haptics fixed out of the capsular bag were not all fixed at the ciliary grooves. (3) After postoperative 3 months, the residual cortex of the lens still existed. Too much residual cortex in the eye caused postoperative peripheral iris bombe and narrowing of chamber angle. CONCLUSION: The changes of anterior chamber angle after posterior chamber implantation of IOL are related to the position of IOL haptics and much residual cortex must be avoided.

Adult↗