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Biomedical subjects

X Chardonnens

Publications and source records attributed to X Chardonnens.

13 recordsLinked to original sources
PubMed1983

Human T cell clones allospecific for HLA-DR5 antigen with the OKT8 phenotype.

Human allospecific T-lymphocyte clones reactive in the primed lymphocyte (PLT) and/or the CML assays were established and grown using T cell growth factor and weekly stimulation with a pool of allogeneic feeder cells. Specificity of selected clones was determined by their reactivity with a panel of HLA-typed lymphocytes. The phenotype of the clones was identified by monoclonal antibodies and complement lysis. Two weakly cytolytic clones, which specifically proliferated in response to DR5 bearing lymphocytes in PLT, possessed the OKT8 marker, suggesting that this determinant is not exclusively involved in the recognition of class I antigens.

Antibodies, Monoclonal↗
PubMed1980

Cloned primed-lymphocyte-test reagents in the dissection of HLA-D.

Human T lymphocytes obtained as blasts on day 4 from a primary mixed leukocyte culture (MLC) were cloned in the presence of T cell growth factor (TCGF) and feeder cells. Parameters important in producing higher-specific-activity TCGF were evaluated; irradiation of the responding cells as well as removal of adherent cells or inclusion of indomethacin in the culture was important. In addition, the presence of an irradiated lymphoblastoid cell line (LCL) cell in the TCGF-producing system enhanced activity in the supernate. The long-term maintenance of progeny from clones was achieved by utilizing the LCL autologous with either the responding or sensitizing cells from the initial MLC as feeder cells. Under those conditions, clones could be expanded for 7 or more wk with the maintenance of PLT reactivity. Had all the cells in each clone been maintained for the full 7 wk, more than 1 X 10(10) cells could have been developed in each clone. The cloned reagents provide a higher degree of antigen-specific reactivity than do normal PLT cells. It is to be anticipated that as the requirements for cloning are made more stringent, including the recloning of the cells, these reagents will aid greatly in the dissection of the complexity attendant to HLA-D.

Clone Cells↗
PubMed1980

Lymphocyte-mediated cytotoxicity and humoral antibodies in human pregnancy.

Lymphocyte-mediated cytotoxicity, antibody-dependent cell cytotoxicity and complement-dependent antibody assays were used to study maternal immune response to fetal antigens present on cord lymphocytes at delivery. Among 24 cell-mediated lympholysis tests, 4 were found slightly positive and accompanied by a complement-dependent antibody and/or an antibody-dependent cell cytotoxicity response. The humoral immune response was found more often positive than the cellular response.

Antibodies↗
PubMed1980

Immunobiology of pregnancy: evidence for a fetal immune response against the mother.

In a study of 11 pregnancy sera, four retroplacental sera were found to contain antibodies directed against the current pregnancy. In two cases, cord sera were also positive. Surprisingly, the first serum contained antibodies apparently directed against the mother but not the father, thus suggesting an immune response of the fetus. Interestingly, the second was also positive despite the fact that it was a primigravida.

Antibodies↗
PubMed1979

A screening program for anti-DR typing reagents.

A total of 694 sera have been tested in a screening program aimed at identifying monospecific reagents for HLA--DR typing. All sera were first tested on a panel of enriched B and T cells without absorption on platelets. Only sera reacting more frequently on B than on T lymphocytes were absorbed on platelets and retested on the panel. This procedure saved a considerable amount of platelets and proved to be reasonably efficient. One-hundred-and-fifty sera were found to contain an anti-B cell antibody. Significantly less frequent B cell reactivity was found when HLA--A, --B, --C antibodies could not be detected. Twenty-five sera were demonstrated to be specific for well-defined DR antigens.

Antibodies, Anti-Idiotypic↗
PubMed1977

[HL-A D antigens from B-lymphocytes and susceptibility to certain diseases].

The discovery of many associations between HLA and human diseases has emphasized the biologic importance of the main histocompatibility system in man. The recent findings from specific immune response genes (Ir locus) mapping within the H2 region of the mouse have led to systematic study of the similar D locus mapping within the HLA region in man. In this study the frequency of a number of HLA-D antigens has been determined in normal individuals and in patients with four diseases selected in view of their genetic background: juvenile diabetes, multiple sclerosis, grass pollinosis and acute leukemia. In each a significant association has been found with a specific HLA-D antigen: DW3 in juvenile diabetes and grass pollinosis, DW2 in multiple sclerosis, and DW7 in acute lymphoblastic leukemia.

B-Lymphocytes↗