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Biomedical subjects

X Bian

Publications and source records attributed to X Bian.

At least 37 records · Page 2Linked to original sources
PubMed2000

[The effect of nordihydroguaiaretic acid on endothelial cell migration induced by glioma cells].

OBJECTIVE: To investigate the effect of nordihydroguaiaretic acid (NDGA) on the migration of endothelial cells (ECs) induced by glioma cells (GCs) in vitro. METHODS: Cells of human umbilical vein endothelial cell line ECV-304 and human malignant glioma cell line SHG-44 were co-cultured in the Falcon Cell Culture Insert system, and the effect of NDGA on the migration of ECs induced by GCs was investigated. The expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) was examined with immunohistochemistry. RESULTS: The expressions of VEGF and bFGF in SHG-44 cells were reduced after treatment with 100 micromol/L NDGA for 1 to 3 days. 100 micromol/L NDGA significantly inhibited not only the chemotactic migration of ECs induced by either glioma cells or glioma-conditioned media, but also the random motility of ECs (P < 0.01). NDGA at a concentration between 50 micromol/L and 200 micromol/L inhibited the chemotactic migration of ECs in a dose-dependent manner (the inhibition ratios were 12.0%, 37.4%, 86.7% and 82.2%). CONCLUSION: The results confirm the inhibitory effects of NDGA on the migration and angiogenic factor expression of ECs, which suggests that NDGA may suppress angiogenesis of glioma.

Brain Neoplasms↗
PubMed2000

[The experience with endoscopic natural ostiotomy].

OBJECTIVE: To improve the operative technique of endoscopic middle metal antrostomy. METHODS: One hundred and thirty-five patients with chronic sinusitis were divided into two groups(first stage and second stage groups). All of them had been followed up for more than 6 months. RESULTS: Middle metal antrostomy was performed for 79 patients (158 sides) in the first group. The diameter of middle meatal window ranged from 1.0 cm (anterior-posterior diameter) to 1.5 cm(superior-inferior diameter). Improved middle meatal antrostomy was performed for 56 patients (112 sides) in the second group. The anterior-posterior diameter of the middle meatal window was over 1.0 cm, and superior-inferior diameter was over 1.5 cm. In the first group, the patency rate in the first stage was 78.48% (124/158), and satisfactory patency rate was 43.0% (68/158). The patency rate was 85.4% (135/158), and satisfactory patency rate was 45.0%(71/158). In the second group, the patency rate was 92.9%(104/112) in the first stage, and 94.6%(106/112) in the second stage. The satisfactory patency rate in both stages was 79.5%(89/112). There was significant difference between two groups(P < 0.05). CONCLUSION: The patency rate and satisfactory patency rate of middle meatal antrostomy are increased by improved technique.

Adolescent↗
PubMed2000

[Insulin-like growth factor-I(IGF-I), and its binding protein-3 (IGFBP-3) correlated with fetal development].

OBJECTIVE: To study the relations between IGF-I, IGFBP-3 and human fetal growth. METHODS: The blood samples of maternal serum (MS) and umbilical cord serum (UCS) from 81 cases with singleton term pregnancy including 38 cases of normal pregnancy (NP), 20 cases of gestational diabetic mellitus (GDM), 23 cases of macrosomia of non-diabetic pregnancy (MNDP) were examined for IGF-I and IGFBP-3 levels by immunoassay kit from DSL, USA. RESULTS: IGF-I in MS of NP and MNDP showed a positive correlation with birth weight (BW) (r = 0.653 and r = 0.640 respectively, both P < 0.01). IGF-I of MS in MNDP was higher than that in NP (P < 0.05). IGF-I of MS and UCS in GDM were higher than that in NP (P < 0.05). IGF-I and IGFBP-3 of UCS in these three groups were significantly lower than that of MS (P < 0.001). CONCLUSION: Detection of IGF-I in MS can assess fetal intra-uterus growth, birth weight and has certain value of predicting macrosomia. IGF-I and IGFBP-3 play important roles for human fetal growth.

Adult↗
PubMed2000

[Papanicolaou test in pregnancy].

OBJECTIVE: To investigate the necessity, safety and acceptability of routine Papanicolaou (Pap) smear in pregnant women and cytological characteristics of the smears during pregnancy. METHODS: Pap smear were performed in 954 pregnant women, 1,053 normal controls and 1,908 gynecological out-patients. The cytological preparations were analyzed by using PAPNET method and following Bethesda (TBS-NCI) diagnostic criteria. Quality control was carried out by following TBS-NCI standard. RESULTS: Abnormal epithelial change was found in 89 women (89/954, 9.3%) of pregnant group, which was quite similar to normal controls group and gynecological group. Occurrence rate of cervical squamous intraepithelial lesion (SIL) was the lowest in pregnant group (0.94%, 9 in 954 cases), comparing with control group (2.75%) and gynecological group (6.23%). However, pregnant group had the highest infection rate (89.9%), as compared to gynecological group (64.5%), and normal controls group (69.1%) (P < 0.01). CONCLUSIONS: Cervical Pap smear test is necessary, safety and acceptable for routine prenatal tests. Any pregnant women with infections must be treated as nonpregnant one.

Adult↗
PubMed1999

Right coronary pressure modulates right ventricular systolic stiffness and oxygen consumption.

OBJECTIVE: The low transmural pressure of the thin right ventricular (RV) wall may render it responsive to right coronary (RC) pressure-induced changes in systolic stiffness and account for the dependence of RV MVO2 on RC pressure (RCP). METHODS: In eight dogs anesthetized with pentobarbital and fentanyl, RCP was lowered from the baseline to 30 mmHg in 10 mmHg steps by adjusting an occluder on the proximal RC. Myocardial segment length and isometric developed force were measured, and the slope of the force-length curve during ejection period (delta F/ delta SL) was used as an index of systolic myocardial stiffness. MVO2 was calculated from RC flow and arteriovenous O2 difference. RESULTS: As RCP was varied from 120 to 40 mmHg with positive lactate uptake, RC flow, maximum developed force (Fmax), delta F, and MVO2 decreased linearly, whereas end-diastolic length, delta SL, and other hemodynamic variables stayed constant. Thus, RV systolic stiffness fell linearly with RC pressure. When RCP was further lowered from 40 to 30 mmHg, Fmax and delta F continued to fall, end-diastolic segment length and right atrial pressure increased significantly, delta SL and RV dp/dtmax fell significantly, and delta F/delta SL reached its lowest value. RV systolic stiffness was 22% of previously reported left ventricular systolic stiffness for coronary perfusion pressure at 100 mmHg, and varied less steeply with coronary pressure. CONCLUSIONS: (1) Reductions in RV systolic stiffness preserve delta SL as coronary pressure is reduced over a wide range. (2) The resulting increase in RV efficiency reduces oxygen demand as oxygen supply is reduced, so ischemia is avoided. (3) RV systolic stiffness is much less than left ventricular stiffness, consistent with their anatomical and functional differences.

Animals↗
PubMed1999

A method for producing regional hypoglycemia in blood perfused tissue.

Numerous studies have focused on the metabolic contributions of glucose and other substrates in isolated tissue preparations by examining the effects of eliminating glucose from the physiologic perfusate or bath solution. To date, however, an effective method of glucose removal from the blood supply to selected tissue in the whole animal model has not been available. We have developed a method for blood glucose removal by continuous flow dialysis. This method was used to generate isolated coronary hypoglycemia for an investigation of myocardial metabolic substrate selection during hypoperfusion in open-chest, anesthetized dogs. Arterial blood was passed through the dialysis system against an isotonic and physiologic dialysate solution prior to controlled coronary perfusion. During normal perfusion pressure (100 mmHg), with a coronary blood flow of 32+/-4 ml/min, arterial blood glucose was reduced from 3.26+/-0.31 to 0.54+/-0.14 mM. When blood flow was reduced to 12+/-3 ml/min with lower perfusion pressure (40 mmHg), dialysis reduced arterial glucose from 3.53+/-0.36 to 0.15+/-0.03 mM. We conclude that this is an effective method for producing regional hypoglycemia.

Animals↗
PubMed1999

A method for collecting right coronary venous blood samples from conscious dogs.

This report describes for the first time a technique to collect right coronary venous blood samples from conscious dogs. Catheters, prepared from Micro-Renathane tubing, were surgically implanted in right ventricular superficial veins of three anesthetized dogs. Also implanted were an arterial catheter, a right coronary flow transducer, and a right coronary artery constrictor. The coronary catheter was introduced at a venous bifurcation so that its side holes were positioned above the bifurcation; both ends of the catheter were exteriorized. Heparinized saline was continuously infused through the venous catheter by a battery-powered pump. The dogs were maintained for 10-13 days after surgery, and all catheters remained patent. Multiple right coronary venous samples were collected from each dog. These samples were analyzed for venous oxygen tension (PvO2) under baseline conditions, with right coronary pressure reduced to 50 mmHg, and during the reactive hyperemia after release of the right coronary artery constriction. PvO2 was 27.7 +/- 1.0 mmHg at baseline, 23.4 +/- 1.0 mmHg during coronary artery constriction, and 34.3 +/- 1.5 mmHg during reactive hyperemia. These data and the position of the catheter at autopsy demonstrated that coronary venous blood had been sampled.

Animals↗
PubMed1999

[Observation of the long-term serum insulin level in women with gestational diabetes history].

OBJECTIVE: To determine beta-cell's function by measuring blood glucose level and serum insulin level in women who had history of gestational diabetes mellitus (GDM), but had no long-term diabetes. METHODS: 30 cases in GDM history group, 29 cases in abnormal oral glucose tolerance test(OGTT) history group, and 38 cases in control Group were followed up to undergo fasting blood sugar test and 75 g glucose load test, and to measure their insulin levels. All the cases had no long-term diabetes. RESULT: (1) the blood glucose levels at 2 hour in GDM history group and abnormal OGTT history group were higher than that in control group especially the former, which was very significantly higher (P < 0.001). (2) The insulin at 2 hours in GDM history group was higher than that in control group (P = 0.00067). CONCLUSION: Though women with GDM history do not have long-term diabetes, insulin resistance still exists, postings a possible sign for future diabetes attack.

Adult↗
PubMed1999

[Study on thrombomodulin and plasminogen activator inhibitor type-1 in patients of pregnancy induced hypertension].

OBJECTIVE: To study the circulating levels of Thrombomodulin (TM) and Plasminogen activator inhibitor type-1 (PAI-1) in women with pregnancy induced hypertension (PIH) and normal pregnant women. METHODS: Blood samples were withdrawn from 73 pregnant women, In which 25 mild PIH, 26 moderate PIH, 22 severe PIH were divided into study group, and 24 normotensive healthy pregnant women in their third trimester were control group. The determination of TM was measured by ELISA and PAI-1 by Colorimetric Assay methods. RESULTS: Circulation levels of TM and PAI-1 were increased with increasing severity of the PIH. There were no significant differences between groups of mild PIH and normal pregnancy (TM 45.69 +/- 15.15 micrograms/L and 40.14 +/- 15.82 micrograms/L, PAI-1 22.06 +/- 12.13 kU/L and 18.40 +/- 9.78 kU/L respectively). The parameters significantly increased in moderate (TM 52.76 +/- 13.91 micrograms/L, PAI-1 25.77 +/- 9.45 kU/L) and severe PIH (TM 64.84 +/- 15.16 micrograms/L, PAI-1 30.79 +/- 11.64 kU/L) groups. CONCLUSIONS: These results demonstrate that the endothelial cell damage and its functional disturbance play a very important role in the etiology of PIH. TM and PAI-1 may serve as clinical markers for the assessment of endothelial damage in PIH.

Adult↗
PubMed1999

[Expression of angiogenic factors and cell cycle regulation factors in human glioblastoma cell line SHG-44].

OBJECTIVE: To investigate the biological features and immunophenotypes of human glioblastoma cell line SHG-44 after long term passage. METHODS: Immunohistochemistry and in situ hybridization were used to study the proliferative activity, intermediate filament protein coexistence, expressions of oncoprotein, angiogenic factors and cell cycle regulation factors. RESULTS: After 130 to 150 passages, SHG-44 cells were weakly positive for glial fibrillary acidic protein (GFAP), but strongly positive for vimentin. The labeling index of Ki-67 and PCNA were 83.5% +/- 10.2% and 70.0% +/- 18.7% respectively. Overexpression of p21 ras, c-erbB-2, epidermal growth factor (EGF) and EGF receptor were obtained. Basic fibroblast growth factor (bFGF), FGF receptor, vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) were also up-regulated in this cell line. p16, p53, cdk4 and cyclin D1 could be detected in the cells and their indices were 43.1% +/- 11.2%, 20.7% +/- 6.6%, 33.1% +/- 11.4% and 29.2% +/- 4.7% respectively. CONCLUSION: Expressive abnormalities of these growth factors, their receptors and the above oncoproteins as well as disorders of cell cycle regulation contribute to the rapid growth and high degree of malignancy of this cell line.

Angiogenesis Inducing Agents↗
PubMed1998

Right coronary autoregulation in conscious, chronically instrumented dogs.

Right coronary (RC) autoregulation and right ventricular (RV) function were assessed in conscious dogs, chronically instrumented to measure RC flow and RC pressure (RCP) as a hydraulic occluder on the RC was inflated. Dogs were then anesthetized, and RC autoregulation and RV function were again assessed. In the conscious state, moderate RC autoregulation was present with closed loop gains (Gc) of 0.59-0.27 as RCP was reduced from 100 to 40 mmHg. In the anesthetized state, Gc was not significantly less than in the conscious state at RCP >50 mmHg. The range and potency of RV autoregulation were greater in both groups than for previously reported findings in anesthetized dogs with RC perfused by an extracorporeal system. RV contractile function was well maintained in conscious and anesthetized dogs at RCP >45 mmHg. We conclude the following: 1) modest RC autoregulation is present in the conscious dog, 2) anesthesia limits the range but not the degree of RC autoregulation, 3) extracorporeal perfusion systems appear to depress RC autoregulation, and 4) RV contractile function remains constant in both conscious and anesthetized dogs until RCP falls below 50 mmHg.

Anesthesia, General↗
PubMed1998

[Transvaginal ultrasound assessment of cervical changes before onset of labor].

OBJECTIVE: To study the clinical value of cervical measurement by transvaginal ultrasound after 36 weeks of gestation. METHODS: Eighty three normal nulliparous woman were studied from 36 weeks of gestation until delivery prospectivelly. Transvaginal ultrasound examinations were performed and cervical length (CL), cervical width (CW) and the diameter of cervical canal were measured weekly. 52 cases were delivered naturelly and 31 cases by cesarean sections (CS). RESULTS: From 36 weeks of gestation until delivery, there was a significant correlation between gestation age (GA) and CL and the diameter of cervical canal (r = -0.37, P < 0.00001; r = 0.16, P = 0.002). The time from examination to initiation of labor was significantly correlated with CL and the diameter of cervical canal (r = 0.32, P < 0.00001; r = -0.19, P = 0.02). The regressive formulas were CL (cm) = 13.77 - 0.27 x GA, the time to beginning of labor (week) = 0.64 + 0.54 x CL (cm). The cases with CL > or = 3.5 cm at 2-3 weeks before labor had a significant higher CS rate than those with CL < or = 3.5 cm (P = 0.002). CONCLUSIONS: Transvaginal ultrasonographic measurement of CL, CW and the diameter of cervical canal could reflect the maturity of cervix accurately. It could be used as predictors of labor course and outcome of delivery.

Adult↗
PubMed1998

[Prediction of cephalopelvic disproportion by ultrasonographic cephalopelic].

OBJECTIVE: To develop a prospective antepartum method of identifying cephalopelvic disproportion by comparing the diameters of fetal head with those of the maternal midpelvis. METHODS: Transvaginal ultrasound pelvimetry was performed on 190 healthy primigravidas with cephalic presentation at 28-35 weeks of gestation, and the diameters of their fetal heads were meassured within one week prior to delivery. These indices the cephalopelvic indices of diameter, cirumference and area, were calculated and compared. RESULTS: The cephalopelvic index of diameter (CID), defined as the difference between the mean diameter of the midpelvis and the fetal biparetal diaameter (BPD), showed the highest degree of accuracy (77.9%). Eighty three percent of women with CID less than 15.8 needed operative delivery; 76.2% of those with CId more than 15.8 mm underwent vaginal delivery. CONCLUSIONS: Transvaginal ultrasound pelvimetry and the CID by and large seems to be able to identify cephalopelvic disproportion before labor and may help obsetricians choose the most appropriate form of delivery in an uncomplicated vertex presentation.

Adult↗
PubMed1998

Prevention of maternal-fetal blood group incompatibility with traditional Chinese herbal medicine.

OBJECTIVE: To evaluate the effect of traditional Chinese herbal medicine on the prevention of hemolytic disease caused by maternal-fetal blood group incompatibility. METHODS: A total of 126 Chinese patients with maternal-fetal blood group incompatibility were studied, including 105 cases of ABO type and 21 cases of Rh type incompatibility. The traditional Chinese herbal medicine was used for preventative treatment in 79 cases, and 47 cases served as controls. The prescription consisted of Herba leonuri 500 g, white peony root 180 g, Banksia rose 12 g, root of Chinese angelica 150 g and Rhizomal ligustica 150 g. RESULTS: Preventative treatment with traditional Chinese herbal medicine significantly reduced the perinatal mortality rate in cases of Rh type incompatibility, from 50.0% in the control group to 7.7% in the treatment group (P < 0.05). There was no death in the cases of ABO incompatibility. The traditional Chinese herbal medicine also decreased the degree of hemolysis. The percentage of severe cases in the control group was 29.8%, while it was 15.2% in the treatment group (P = 0.05). The duration of treatment was closely related to the outcomes. In patients who used traditional Chinese herbal medicine for more than 10 weeks, the incidence of severe hemolysis was 8.2%, compared with 38.9% in those treated for less than 10 weeks (P < 0.05). The change of serum immune antibody (A and/or B) titers during the treatment was a very important predictive factor for the outcome of the pregnancy. CONCLUSION: Traditional Chinese herbal medicine is effective not only for ABO type but also for Rh type maternal-fetal blood group incompatibility, with no side effects.

ABO Blood-Group System↗