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X A Li

Publications and source records attributed to X A Li.

51 records · Page 3Linked to original sources

Electron mass scattering powers: Monte Carlo and analytical calculations.

Values of electron mass scattering power, T/p, for various materials have been calculated by using the EGS4 Monte Carlo system and by integration of the Molière multiple-scattering distribution. The energy range covered is 0.5-100 MeV. Monte Carlo calculations test the concept of T/p "experimentally" and assess the contribution to electron mass scattering power from effects such as Moller scatter and energy-loss straggling. The Monte Carlo results agree within 2% with the analytical results calculated from Molière multiple-scattering theory at energies less than 20 MeV for high-Z materials and for energies less than 50 MeV for low-Z materials. At higher energies the Monte Carlo calculations include the effects of bremsstrahlung production which can significantly increase values of T/p. For low-Z materials and electron energies less than 60 MeV, the Monte Carlo calculated T/p values are generally 22% higher than those given by ICRU Report 35, while those for high-Z materials and energies less than 25 MeV are found to be consistent (within 1%) with ICRU Report 35. The effects of Moller scatter, which significantly affect T/p for low-Z materials, as well as bremsstrahlung effects, are included in the present Monte Carlo calculations. If the tabulated T/p data of ICRU Report 35 are modified to include the Moller scatter effect, then for energies less than 60 MeV they are generally 6% less than the present Monte Carlo data for low-Z materials as well as for copper. It is shown that T/p is a well-defined constant over an appropriate range of slab thickness except when bremsstrahlung effects are significant. It is found that T/p is proportional to E-n, where n is in the range of 1.5-2.0 for the energies considered here. The Monte Carlo calculations are shown to agree well with various relevant experimental measurements. Accurate T/p data, which should include the effect of Moller scatter, are necessary in electron-beam treatment planning, especially for a small field size. The choice of the depth step in the implementation of pencil-beam codes should not violate the slab-thickness limits for T/p data.

Electrons↗

Characterization of serum amyloid P component from human aortic atherosclerotic lesions.

Serum amyloid P component (SAP) is a glycoprotein in human plasma. We recently showed the localization of SAP in human atherosclerotic lesions by immunohistochemical staining. In this study, the presence of SAP in atherosclerotic lesions was confirmed, and the biochemical character of SAP in atherosclerotic intima was investigated and compared with that of native SAP. Atherosclerotic intima was sequentially extracted with 2 mmol/L CaCl2-Tris-buffered saline (TBS), 10 mmol/L EDTA-TBS, 3 mol/L guanidine-TBS, and collagenase digestion. The character of SAP in each extract was studied with double immunodiffusion, electroimmunoassay, crossed immunoelectrophoresis, and Western immunoblotting. The total amount of SAP in atherosclerotic intima was 190 +/- 64 micrograms/g wet tissue with an SAP-albumin ratio of 1:22.7, which is 44 times higher than the relative plasma ratio of 1:1000. This suggests that SAP is specifically localized in atherosclerotic lesions. SAP from the intima was indistinguishable from plasma or purified SAP with respect to immunological character and molecular weight. However, electrophoretic mobility and the binding of SAP to atherosclerotic intima appeared heterogeneous. Of total extractable SAP, about 43% appeared in the CaCl2-TBS fraction, 25% in the EDTA-TBS fraction, and 32% in the collagenase digestion fraction. SAP is one of the two pentraxins in human plasma; the other is C-reactive protein, which has also been reported to locate in atherosclerotic lesions. Our findings suggest a role for SAP in atherogenesis and encourage efforts to determine more precisely the physiological contributions of the pentraxin family to the development of atherosclerosis.

Aged↗

Binding of serum amyloid P component to heparin in human serum.

It has been proposed that the function of serum amyloid P component (SAP) may closely relate with its binding to polysaccharides, especially glycosaminoglycans. We employed a quantitative immunoelectrophoresis (QIE) method and a native polyacrylamide gel electrophoresis (PAGE) method to characterize the SAP-heparin binding in soluble state. The SAP-heparin binding showed positive cooperativity. The apparent numbers of heparin molecules bound to SAP varied with the calcium concentration with a ratio of 1:1 (SAP/heparin), a Kd of 2.06 x 10(-7) M at 0.1 mM CaCl2 and a ratio of 1:1.6 (SAP/heparin), a Kd of 3.91 x 10(-7) M at 2 mM CaCl2, when estimated by the QIE method. No binding between SAP and heparin was observed in the absence of calcium. Magnesium and barium failed to induce the formation of SAP-heparin complex. Furthermore, they showed inhibitory effects on the calcium-mediated complex formation. We propose that heparin might be a regulator to modulate the anticoagulant activity of SAP and a useful drug to prevent SAP deposition on amyloid deposits.

Barium↗

A case report of deficiency in an inhibitor of calcium-dependent association of protein S with C4B-binding protein suggested by a modified crossed immunoelectrophoresis.

We have experienced a coagulation factor VIII-deficient patient whose plasma has normal protein S (PS) activity and masses of free PS and its bound form in complex with C4b-binding protein (C4BP). Although the patient's plasma showed a normal ratio of free PS to PS-C4BP complex in the presence of 5 mM EDTA, the plasma gave an abnormally retarding major C4BP peak together with a major PS peak in the crossed immunoelectrophoresis (CIE) in the presence of 2 mM CaCl2. It was revealed that the major peak was formed by a mixture of PS-C4BP complex and free form. The addition of normal human plasma (NHP) to the patient's plasma inhibited the retardation of the major PS-C4BP complex. These suggest that the patient's plasma lacks some component(s) to inhibit Ca(2+)-dependent association of PS with C4BP.

Adult↗

Reducing electron contamination for photon beam-quality specification.

The percentage depth dose at 10 cm in a 10 x 10-cm2 photon beam at an SSD of 100 cm, %dd(10), is a better beam-quality specifier for radiotherapy beams than the commonly used values of TPR10(20) or nominal accelerating potential. The presence of electron contamination affects the measurement of %dd(10) but can be removed by the use of a 0.1-cm lead filter, which reduces surface dose from contaminant electrons from the accelerator by more than 95% for radiotherapy beams with energies from 60Co to 50 MV. The filter performs best when it is placed immediately below the head. An electron-contamination correction factor is introduced to correct for electron contamination from the filter and air. It converts the %dd(10) which includes the electron contamination with the filter in place [hereafter %dd(10)m], into %dd(10) for just the photons in the filtered beam. The correction factor is a linear function of %dd(10)m for all filtered beams with %dd(10)m > 70%. A small correction for the photon filtering effect converts the pure photon %dd(10) for the filtered beam into that for the unfiltered beam, which can be used to determine stopping-power ratio. Calculations show that the values of water-to-air stopping power ratio in the unfiltered beam are related to the values of %dd(10)m in the filtered beam by a cubic function. The uncertainty of stopping-power ratios in unfiltered beams for the same value of the %dd(10)m is within 0.2% for all beams.

Cobalt Radioisotopes↗

Sequence-specific 1H NMR assignments and solution structure of bovine pancreatic polypeptide.

Sequence-specific 1H NMR assignments for the 36 residue bovine pancreatic polypeptide (bPP) have been completed. The secondary and tertiary structure of bPP in solution has been determined from experimental NMR data. It is shown that bPP has a very well-defined C-terminal alpha-helix involving residues 15-32. Although regular secondary structure cannot be clearly defined in the N-terminal region, residues 4-8 maintain a rather ordered conformation in solution. This is attributed primarily to the hydrophobic interactions between this region and the C-terminal helix. The two segments of the structure are joined by a turn which is poorly defined. The four end residues both at the N-terminus and the C-terminus are highly disordered in solution. The overall fold of the bPP molecule is very closely similar to that found in the crystal structure of avian pancreatic polypeptide (aPP). The RMS deviation for backbone atoms of residues 4-8 and 15-32 between the bPP mean structure and the aPP crystal structure is 0.65 A, although there is only 39% identity of the residues. Furthermore, the average conformations of some (mostly from the alpha-helix) side chains of bPP in solution are closely similar to those of aPP in the crystal structure. A large number of side chains of bPP, however, show significant conformational averaging in solution.

Amino Acid Sequence↗

[Immunological and hematopoietic effect of Codonopsis pilosula on cancer patients during radiotherapy].

UNLABELLED: Codonopsis pilosula was used as an adjuvant in 76 cancer patients during radiotherapy and its protective effect on hematopoietic and immunologic function was studied. RESULTS: (1) No influence on Hb and WBC of the patients with radiotherapy. (2) It could reduce the immunosuppressive effect of radiotherapy on delayed hypersensitive reaction, the lymphocyte response to PHA and IL-2. (3) No difference between treated and control groups in most humoral immune indices such as IgG, IgA and C3, but had slight increase in IgM in treated patients, while significant decrease in control.

Aged↗

Messenger RNA stabilization in chicken lens development: a reexamination.

Previous studies, using actinomycin D, suggested that the rate of mRNA degradation in chicken embryo lens epithelial cells decreased sharply between Days 11 and 13 of development (Yoshida and Katoh, Exp. Eye Res. 11, 1971; Exp. Cell Res. 71, 1972). We repeated these studies and directly measured the effects of actinomycin on lens mRNAs. Although actinomycin decreased [3H]uridine incorporation greater than 90%, only modest decreases in methionine incorporation were detected. Treatment for 6 hr had no detectable effect on delta-crystallin mRNA levels or on the relative amounts of proteins synthesized in an in vitro translation system. Unlike the previous studies, we did not find significant changes in the stability of lens epithelial mRNAs during development.

Animals↗

Competitive inhibition of a set of endoplasmic reticulum protein genes (GRP78, GRP94, and ERp72) retards cell growth and lowers viability after ionophore treatment.

GRP78, a 78-kDa protein localized in the endoplasmic reticulum (ER), has been implicated in protein processing and stress protection. Its promoter contains a 36-bp region which is conserved among GRP genes across species and has the ability to compete for trans-acting factors mediating GRP gene expression. Integration of about 800 tandem copies of this sequence into the genome of a Chinese hamster ovary cell line (DG44) results in transfectants with the following phenotypes: (i) the induction level of GRP78 by the calcium ionophore A23187 and tunicamycin is reduced 4- and 2-fold, respectively, (ii) the induction levels of two other ER luminal protein genes, GRP94 and ERp72, are simultaneously down-regulated, (iii) the growth rate of these cells is half that of transfectants without the amplified sequence, and (iv) cell viability is decreased by 25-fold after A23187 treatment. These results provide new evidence that ERp72 shares common trans-acting regulatory factors with the GRP genes and that a reduction of this set of ER proteins correlates with lower viability after ionophore treatment.

Animals↗

The rat 78,000 dalton glucose-regulated protein (GRP78) as a precursor for the rat steroidogenesis-activator polypeptide (SAP): the SAP coding sequence is homologous with the terminal end of GRP78.

Based on the striking sequence identity between the amino acid sequence of rat steroidogenesis-activator polypeptide (SAP) and the carboxyl terminus of the 78,000 dalton glucose-regulated protein (GRP78), the precursor-product relationship between GRP78 and SAP was investigated in Leydig cells. Immunoblot analysis with peptide antibodies specific for GRP78 and SAP showed that the putative SAP precursor is also immunoreactive with the anti-GRP78 antibody. Genomic blot hybridizations further revealed that GRP78 is neither rearranged nor amplified in the H-540 Leydig cell tumor, the original source for SAP. Further, there appears to be a single copy of the SAP coding sequence within the rat genome. This sequence resides within the last exon of GRP78. Our observations support the hypothesis that, in steroidogenic cells, SAP is likely to be derived from posttranslational processing of a very minor fraction of GRP78.

Amino Acid Sequence↗

[In vitro assessment of candidacidal activity of human leukocyte myeloperoxidase preparation].

Myeloperoxidase (MPO), present in the azurophilic granules of human polymorphonuclear neutrophils, is important in the oxygen-dependent microbicidal activity of neutrophils. The purpose of our study was to investigate the therapeutic potency of the MPO preparations. This paper is to present our primary work on MPO isolation and its microbicidal activity assay. White blood cells, isolated freshly from normal donors, were lysed with cetyltrimehylammonium bromide to liberate myeloperoxidase. The enzyme preparation was partially purified by 50% (NH4)2SO4 precipitation followed by 65% (NH4)2SO4 precipitation. A hundred million leukocytes yielded 1.03 mg protein of the MPO preparation with the RZ of 0.31. The specific activity of the MPO preparation was about 29.25 u/mg. When 0.672 units of the MPO preparation were incubated with about 10(7) clinical isolates of Candida albicans in the presence of 0.2 mmol/L H2O2 and 0.14 mol/L NaCl. It was detected that 95.58 +/- 0.64% of the organisms were killed in the methylene blue staining system.

Antifungal Agents↗

[Renal function in chronic cor pulmonale monitored with beta 2-microglobulin].

Serum and urine levels of beta 2-microglobulin (beta 2-MG) were measured with radioimmune assay in 40 chronic cor pulmonale patients (52 episodes) and 26 normal controls. The results showed that the serum and urine levels of beta 2-MG were 2.01 +/- 0.47 mg/L and 0.10 +/- 0.08 mg/L respectively, in the normal controls, while in the cor pulmonale group 3.86 +/- 1.58 mg/L and 0.66 +/- 0.34 mg/L respectively, with statistical significance (P less than 0.001, less than 0.005). It was shown that there was a positive correlation between the levels of serum and urine beta 2-MG and PaCO2 and a negative correlation between the levels of serum and urine beta 2-MG and PaO2. The results indicated that determination of serum and urine beta 2-MG could be used in the early detection of the renal impairment in chronic cor pulmonale. The predisposing factors of renal dysfunction in cor pulmonale were also discussed.

Adult↗

Dosimetry of source stepping for intravascular brachytherapy.

PURPOSE: Both beta and gamma sources of fixed length are currently used in the catheter-based intravascular brachytherapy (IVBT). Source stepping is often used to treat a lesion longer than the effective treatment length of the source. A major challenge for the stepping procedure is to attain a perfect dosimetric match (uniform dose) at the source junction. This work presents a quantitative and systematic dosimetric analysis for source stepping during an IVBT procedure. MATERIALS AND METHODS: The three most commonly used beta and gamma sources (192Ir by BEST, 90Sr by NOVOSTE and 32P by Guidant) were studied using the EGSnrc Monte Carlo code. Dose distributions were calculated for a perfect end-to-end match and for a range of end-to-end gaps and overlaps between consecutive steps. RESULTS: It is found that a perfect end-to-end match during source stepping yields uniform dose distribution in the region of source junction. The doses in the case of a mismatch (in the presence of an end-to-end gap or overlap) were found to be significantly different from those with the perfect end-to-end match. The dose deviation depends on the size of the gap or overlap, radial distance and type of source. The dose deviation decreases with radial distance for a given gap/overlap. For example, for a gap/overlap of 2 mm, dose decreases/increases of 30%, 55% and 60% were found at the radial distance of 2 mm from source for 192Ir, 90Sr and 32P, respectively. These dose deviations are reduced by approximately 10% when the radial distance increases from 2 to 3 mm. The dose deviations for gaps or overlaps in the range of 0-5 mm are presented. CONCLUSIONS: During an IVBT procedure involving source stepping, a perfect end-to-end match is always desired. Significant underdosing or overdosing can occur in the case of a source mismatch. A considerable caution should be exercised to ensure that sources are properly matched.

Beta Particles↗

ent-kauranoid diterpenes from Artemisia sacrorum.

A new ent-kauranoid diterpene diglycoside has been isolated from Artemisia sacrorum along with two known ent-kauranoid diterpenes. Their structures are elucidated by means of nmr analysis (DEPT, 1H-1H COSY, 1H-13C COSY, and 1H-1H decoupling).

Diterpenes↗