Biomedical subjects

Wei Zhang

Publications and source records attributed to Wei Zhang.

At least 289 records · Page 16Linked to original sources

PubMed2006

[Ultrastructural features and platelet-derived growth factor receptor A gene mutations in CD117-negative gastrointestinal stromal tumor].

OBJECTIVE: To explore the ultrastructural features and mutation status of platelet-derived growth factor receptors A (PDGFRA) and c-kit in gastrointestinal stromal tumors that were immunohistochemically negative for CD117 antigen. METHODS: Six cases of gastrointestinal stromal tumors that were CD117 immunostain negative were studied by electron microscopy. Direct PCR sequencing was used to investigate the mutation status of c-kit gene exons 9, 11, 13, 17 and PDGFRA gene exons 12 and 18. RESULTS: The ultrastructural features of all 6 cases were similar to those of the interstitial cell of Cajal (ICC). None of the 6 cases were found to have c-kit gene mutations. However, three tumors were found to harbor PDGFRA exon 18 activating mutations, including two tumors having an Asp-->Val842 missense mutation and one having an Arg-->Ser841 missense mutation. CONCLUSIONS: PDGFRA mutations may provide an important alternative molecular mechanism for the development of gastrointestinal stromal tumor.

Adult↗

PubMed2006

[Establishment of delta block matching technique].

To establish delta block HLA-matching technique, DNA was extracted from whole blood by salting-out method, delta block was amplified by polymerase chain reaction (PCR), and PCR product was detected by GeneScan. The results showed that delta block had polymorphism in 104 samples without sibship of the Han people from Zhejiang province. The range of DNA fragment length was 81-393 bp and could be divided into 4 groups: 81-118 bp, 140-175 bp, 217-301 bp, 340-393 bp. The numbers of DNA fragments were 6-32. It is concluded that the method of delta block matching is reliable and can be applied to select donors for the patients to be transplanted. It is the first time to get delta block data of the Han people in China.

HLA-A Antigens↗

PubMed2006

[Application of serum surface-enhanced laser desorption/ionization proteomic patterns in distinguishing non-small cell lung cancer patients from healthy people].

OBJECTIVE: To explore the application of serum surface-enhanced laser desorption/ionization (SELDI) marker patterns in distinguishing non-small cell lung cancer patients from healthy people by protein chip technology. METHODS: One hundred and sixty-three serum samples (123 patients with lung cancer and 40 healthy persons), were randomly divided into a training set [94 cases, 53 non-small cell lung cancer (NSCLC), 21 small cell lung cancer and 20 healthy persons] and a blinded test set (69 cases), were included for analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Five protein peaks at 11,493, 6,429, 8,245, 5,336 and 2,536 were automatically chosen for the system training and the development of a decision classification tree model (marker pattern). The accuracy of the model was tested with the blinded test set (an independent set of masked serum samples from 49 patients with NSCLC and 20 healthy persons). RESULTS: The model differentiated the patients with NSCLC from the healthy people with a sensitivity of 95.9% (71/74) and a specificity of 90.0% (18/20) in the training set and a sensitivity of 83.7%, and a specificity of 80.0% in the blinded set respectively. CONCLUSION: SELDI-TOF-MS technique can correctly distinguish NSCLC patients from healthy people, and it has the potential for the development of a screening test for the detection of NSCLC.

Adult↗

PubMed2006

[The effect of siRNA-Her2/neu on the drug sensitivity of Her2/neu-overexpressing lung adenocarcinoma cell line].

OBJECTIVE: To explore the effect of synthesized siRNA targeting Her2/neu oncogene on the drug sensitivity of Her2/neu-overexpressing lung adenocarcinoma cell line. METHODS: The experiments consisted of four groups including an untreated control group, an empty vector group, an unrelated siRNA group, and a Her2/neu siRNA group. Every experiment was repeated five times. Lung adenocarcinoma cell line Calu-3 was transfected with siRNAs formulated LipofectAMINE 2000, and Her2/neu protein and P-gp of each group were determined by flow cytometry (FCM). The chemosensitivity of transfected cells to cisplatin (DDP) was measured by MTT. Cell apoptosis detection kit (Annexin V method) was used to examine the drug induced apoptosis rate. RESULTS: The Her2/neu protein and P-gp positive expression rate in the Her2/neu siRNA group, the untreated control group, the empty vector group and the unrelated siRNA group were [(25.04 +/- 1.56)%, (4.24 +/- 1.01)%], [(98.24 +/- 2.23)%, (5.11 +/- 2.98)%], [(95.67 +/- 1.98)%, (6.98 +/- 2.47)%] or [(94.79 +/- 0.87)%, (5.59 +/- 3.66)%], respectively. Introduction of the sequence specific siRNA into Her2/neu positive Calu-3 cells in vitro greatly reduced the cell surface expression of the Her2/neu protein, but had no effect on P-gp level. Consequently the inhibitory rate of DDP in combination with siRNA targeting Her2/neu was (67.1 +/- 2.3)%, but it was (48.1 +/- 3.5)%, (46.3 +/- 5.9)% and (50.2 +/- 2.9)% in the untreated control, the empty vector and the unrelated siRNA groups, respectively. There was a significant difference between Her2/neu siRNA group and other three groups (P < 0.01). The FCM results showed the apoptosis rate of DDP combined with siRNA-Her2/neu was elevated as compared with the unrelated siRNA group, the empty vector group or the untreated control group. CONCLUSION: Sequence specific siRNA-Her2/neu was capable of enhancing the chemosensitivity of Calu-3 cells to cisplatin.

Adenocarcinoma↗

PubMed2006

[Inhibitory effect of blocking MaxiK channel on human monocyte-derived macrophages differentiation into foam cells].

AIM: To investigate the expression of high-conductance Ca2+-activated potassium channel (MaxiK channel) mRNA and protein during human monocyte-derived macrophage differentiation into foam cells and to study its function in foam cell formation. METHODS: Human peripheral blood monocytes were isolated from healthy male volunteers by density gradient centrifugation and then by adherent method. The obtained monocytes were cultured for 5 days to differentiate into macrophages. Based on establishment of human macrophage-derived foam cells model, the expression of MaxiK channel alpha-subunit was investigated by immunocytochemical staining, RT-PCR and Western blot. Furthermore, the effect of Paxilline, a MaxiK channel-specific inhibitor, on cholesterol metabolism in macrophages incepting oxidized low density lipoprotein (OxLDL) was studied. RESULTS: After the macrophages were co-incubated with 30 mg/L OxLDL at 37 degrees C for 60 hours, the cellular volume obviously enlarged and many red lipid granules were deposited in cytoplasm. The total amount of cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE) in cells markedly increased and the ratio of CE/TC rose from (14.437+/-6.781)% to (57.946+/-3.507)% (n=7, P<0.05). However, the expression of MaxiK channel alpha-subunit had no significant change (P<0.05). Paxilline (5 micromol/L and 10 micromol/L) markedly reduced the content of TC, FC and CE in macrophages and the ratio of CE/TC decreased to (41.217+/-5.584)% (5 micromol/L Paxilline) and (18.017+/-11.559)% (10 micromol/L Paxilline), respectively (n=7, P<0.05). Meanwhile, the red lipid granules deposited in the cytoplasm of macrophages also decreased. CONCLUSION: Blocking MaxiK channel can inhibit human monocyte-derived macrophage to be differentiated into foam cells.

Animals↗

PubMed2006

[The effect of HCV core protein on the expression of cyclooxygenase 2 (COX-2) in HepG2 cells].

AIM: To investigate the effect of Hepatitis C virus (HCV) core protein on the expression of cyclooxygenase 2 (COX-2). METHODS: The genes encoded HCV core protein were amplified from plasmid containing full length genome of HCV strain H77 using PCR, and were cloned into eukaryotic expression vector pcDNA3.1. The recombinant HCV-C/pcDNA3.1 was transiently co-transfected into HepG2 cells with luciferase reporter vector containing COX-2 promotor (COX2pro1.5 kb/luc). The luciferase activity and COX-2 protein expression were detected. RESULTS: The recombinants HCV-C/pcDNA3.1 have been constructed successfully. The luciferase activity of COX-2 promotor was activated by the expressed HCV core, and the increased protein expression of COX-2 in transfected HepG2 cells was detected by Western blot. CONCLUSION: HCV core protein can activate the COX-2 promotor and induce its expression, which provides a new experimental basis for further research on relationship between COX-2 and HCV pathogenesis.

Blotting, Western↗

PubMed2006

[Effects of estradiol intervention on peripheral blood CD90+ cells and cardiac function in de-ovary rats before and after myocardial infarction].

AIM: To investigate the effects of estradiol intervention on peripheral blood CD90+ cells and cardiac function in de-ovary rats before and after myocardial infarction. METHODS: Thirty SD female rats were divided into the control group, acute myocardial infarction group, de-ovary myocardial infarction group, de-ovary replacement group with estradiol, and de-ovary therapy group with estradiol. CD90+ cell percentage was determined with flow cytometry. The left ventricle end systolic pressure (LVESP), left ventricle end diastolic pressure (LVEDP), +dp/dt(max) and -dp/dt(max) were also measured with the POWERLAB4.12 system. RESULTS: The percentage of peripheral blood CD90+ cells in the de-ovary myocardial infarction group was remarkably lower than that in the acute myocardial infarction group and the de-ovary replacement group with estradiol (P<0.01). In the de-ovary replacement group with estradiol, the percentage of peripheral blood CD90+ cells was remarkably elevated following the first day of operation (P<0.05), reaching the peak value at day 3. In the de-ovary therapy group with estradiol, it started to elevate at day 7 after the operation, much lower than that in the de-ovary replacement group with estradiol. LVESP in each operation group decreased, especially in the acute myocardial infarction group, whereas LVEDP increased in the de-ovary group and de-ovary therapy group with estradiol (P<0.01). CONCLUSION: Replacement with estradiol before myocardial infarction exerts more beneficial effects than estradiol replacement after myocardial infarction in de-ovary rats.

Animals↗

PubMed2006

[Histopathology analysis of microemboli after thrombolysis].

OBJECTIVE: To observe the changes in fibrin of microemboli in micro-embolism soon after thrombolysis, in order to provide a theoretical basis for micro-thrombolytic therapy. METHODS: Thirty adult male SD rats weighing (180+/-10)g were randomized into five groups: 5, 30, 60 and 90 minutes groups after micro-thromboembolization and the control group. Rat cremasteric artery was embolized with photochemical method, and micro-thrombi were produced after thrombolysis by staphylokinase. The levels of tissue plasminogen activator (t-PA) and tissue plasminogen activator inhibitor (PAI) were immunohistochemically determined, and taken as the markers of fibrin dissolution. RESULTS: Fibrin was observed in pathologic sections as well as fibrinolytic markers t-PA and PAI in immunohistochemical sections. The levels of t-PA were found to be reduced while that of PAI increased gradually with passage of time. CONCLUSION: Fibrin is one of the main constituents of microthrombi soon after thrombolysis. The content of fibrous emboli increases after microembolization with the passage of time. Thrombolysis plays an important role in the treatment of "no reflow phenomenon".

Animals↗

PubMed2006

[Study on abutment movement of distal removable dentures retained by attachments].

PURPOSE: To study the influence on the abutment movement with three kinds of precision attachments designed for distal-extension removable partial dentures (RPDs). METHODS: Snap attachment, Ceka attachment, Mini-SG attachment were used to retain RPDs. Then food chip was masticated between the upper and lower dentitions on the attachments side. Placement of abutment was measured by LB-72 high-sensitive-laser-traneferring-detector and the data were statistically analyzed for random block analysis of variance. RESULTS: The displacement, inclination, and torsion of the abutment movements in the mesial-distal, bucco-lingual and occluso-gingival directions all had statistical significance (P<0.05). The largest mesial-distal inclination occurred with the Mini-SG attachment. The Ceka attachment produced more abutment displacement in mesial distal and apical directions. Occlusal displacement and anti-clockwise rotation were the least with the Snap attachment. CONCLUSION: The most equitable distribution of stresses on the supporting structures was achieved by Snap attachment with its resilent assemble, Ceka attachment transfers most of the loading forces to the abutments, Mini-SG attachment can not reduce the excessive torque applied to the abutments effectively.

Dental Abutments↗

PubMed2006

[Magnetic resonance imaging for the wrist joint of the coal miners in vibration department].

OBJECTIVE: To study the magnetic resonance imaging (MRI) in the wrist joint of coal miners who work in excavation and vibration department. METHODS: Forty-three coal miners with the hand-arm vibration disease served as the observation group while 20 workers who were not working in the vibration department acted as the control group. The patients in the observation group were divided into five subgroups according to the time when they received vibration. The regularity of the development of signs and symptoms of MRI was observed and analyzed. RESULTS: The hydroarthrosis was most found in MRI. There were significant difference in hydroarthrosis (chi(2) = 8.80, P < 0.01), osteoporosis and osteomyelitis (chi(2) = 3.91, chi(2) = 5.01, P < 0.05 respectively) between the observation group and the control group. The edema of bone marrow and the avascular necrosis of ossa carpi were found only in the observation group and not found in the control group. The hydroarthrosis and the edema of bone marrow occurred most in the early stage of vibration. The signal in the edema of the bone marrow of the distal end of the radius was decreased in the GE sequence T(2)WI with the specificity. CONCLUSION: (1) Changes in the wrist joint occur in the early stage of the vibration work, and can be found in the MRI. (2) The edema of the bone marrow of the distal end of the radius is of great value in the diagnosis of the hand-arm vibration disease.

Adult↗

PubMed2006

[Diagnostic value of 18F-fDG Pet/CT in the detection of the cervical lymph nodes metastasis].

OBJECTIVE: To explore the diagnostic value of 18F-FDG PET/CT in the detection of the cervical lymph nodes metastasis. METHOD: According to the pathological result, the data of neck PET, CT, PET/CT of 20 patients with head and neck tumors were analyzed retrospectively. RESULT: The sensitivity, specificity and accuracy of PET, CT and PET/CT in detection cervical lymph nodes metastasis were (92.3%, 85.7%, 90.0%), (77.0%, 57.1%, 70.0%), (92.3%, 100.0%, 95.0%) respectively, the accuracy of PET/CT was better than that of CT alone (P < 0.05) and a little better than that of PET alone. Among 13 cases after irradiation,the correct diagnose was found in seven cases by CT and in 12 cases by PET/CT. Four cases were found with cervical lymph node metastasis in the seven clinical N0 (cN0) patients after functional neck dissection (FND). The preoperative diagnose of PET/CT was completely consistent with the postoperative pathological result. CONCLUSION: 18F-FDG PET/CT is better than CT alone and PET alone for the detection of cervical lymph nodes metastasis, especially for the recurrent tumors. It may be served as the indication of neck dissection in the head and neck cancers.

Adult↗

PubMed2006

[Study on health related behaviors and its protective factors of junior middle school students in 4 cities of China].

OBJECTIVE: To provide accurate data on health related behaviors and protective factors among students in middle schools in China, for developing priorities, programs and policies on health education. METHODS: We used a standard scientific sample selection process developed by American Center for Disease Control and Prevention (CDC) to conduct the questionnaire survey among middle schools from four cities--Beijing, Hangzhou, Wuhan and Urumchi. RESULTS: Data were found as: 3.2% of students are overweight; 25.3% of students rarely washing hands before eating at school, 20.5% of the students had seriously injured in the past 12 months, 30.4% of male students having had physical fighting, 17.1% of the students having serious attempted suicide, 29.7% of the students ever tried or experimented cigarette smoking, 13.0% having drunk from alcohol and 14.5% having been offered or selling drugs during the past 30 days, 78.9% were in an insufficient amount of physical activity and only 14.3% often used seat belt when riding in a car. CONCLUSIONS: There were many problems on health related behaviors among middle school students in these four cities, especially on hygiene, physical activities, psychological situation, smoking and drinking etc. It is absolutely necessary to develop health education for children and adolescence to promote their healthy behaviors and lifestyle.

Adolescent↗

PubMed2006

[Clinical analysis of 32 primary intestinal non-Hodgkin's lymphoma].

OBJECTIVE: To investigate the clinical and pathological features, optimal treatment and prognostic factors in primary intestinal non-Hodgkin's lymphoma. METHODS: The clinical presentations, pathological features and therapeutic results of 32 primary intestinal non-Hodgkin's lymphoma were retrospectively analyzed. Statistical analyses were performed with SSPS 10.0 software. RESULTS: The most frequently site of the lesions in the 32 patients was the large intestine (n = 16, 50.0%), followed by small intestine (n = 8, 25.0%), ileocaecal region (n = 6, 18.8%) and multiple intestinal sites (n = 2, 6.2%). Clinical presentations were as follows: abdominal pain and/or distention (n = 26, 81.2%); abdominal mass (n = 14, 43.8%); diarrhea (n = 12, 37.5%); melena (n = 10, 31.3%); weight loss (n = 10, 31.3%) and fever (n = 8, 25.0%). Twenty-one patients (65.6%) were diagnosed as B-cell lymphoma, 15 (46.9%) were diffuse large B-cell lymphoma. Ten patients (31.2%) were diagnosed as T-cell lymphoma and one (3.1%) as histiocytic lymphoma. Twenty-nine patients were treated initially by surgery with or without chemotherapy, 19 of them (59.4%) achieved complete response. Based on Cox multivariate analysis, stage III - IV, B symptoms and T cell phenotype of the disease were the independent adverse prognostic factors (P < 0.05). CONCLUSION: The clinical presentation of primary intestinal non-Hodgkin's lymphoma are not specific clinically. Most of the histological types are diffuse large B-cell type lymphoma. Complete resection combined with chemotherapy may be the best effective approach for treatment of this disease. The prognosis of this disease are correlated with the stage, B symptoms and T cell phenotype.

Adult↗

PubMed2006

[Correlation of epididymal protease inhibitor Eppin and Semenogelin on human ejaculated spermatozoa].

OBJECTIVE: To evaluate the correlation of epididymal protease inhibitor(Eppin) and Semenogelin(Sg) on human ejaculated spermatozoa. METHODS: The experimental approaches include: (1) Immunoprecipitation of Eppin with anti-Eppin from semen; (2) Colocalization of Eppin and Sg by immunofluorescence; (3) Immunoprecipitation of rEppin and rSg;(4) Far-Western blotting of rEppin and rSg;(5) Competition of saturated 125I-rSg binding to rEppin with unlabeled Sg, and direct binding of 125I-rSg to rEppin on a blot; (6) Autoradiography of 125I-rSg with rEppin. RESULTS: Eppin-Sg complex present on the surface of human ejaculated spermatozoa, Cys-239 is the only cystein for rEppin binding rSg. Reduction and carboxymethylation of Cys-239 blocks binding of 125I-rEppin to rSg. CONCLUSION: Our study demonstrates that Eppin and Sg bind to each other on human ejaculated spermatozoa. A disulfide linkage occurs between Sg and Eppin, indicating the specificity of binding.

Humans↗

PubMed2006

[Clinical characteristic analysis of the first human case infected by influenza A (H5N1) in Jiangxi Province].

OBJECTIVE: To describe the clinical, laboratory and radiological presentation of a human case infected by influenza A (H5N1), and to understand its management and prognosis. METHODS: The clinical and autopsy data of the first human case infected by influenza A (H5N1) in Jiangxi Province were collected and analyzed. RESULTS: The first case infected by influenza A (H5N1) in Jiangxi Province was confirmed by laboratory findings with reverse transcription-polymerase chain reaction (RT-PCR) and influenza A (H5N1) isolation. The patient had been healthy in the past and exposed to the environment of bird flu before illness. The initial symptoms included high fever with influenza-like symptoms, and then cough and purulent sputum mixed with blood appeared. The clinical situation deteriorated progressively with occurrence of diarrhea and dyspnea. Laboratory abnormalities included decrease of peripheral white blood cells and lymphocytes, urine protein, dramatic increase of enzymes associated with hepatic injury and myocarditis and decrease of serum albumin. Six days later, penicillin-resistant streptococcus pneumoniae was isolated from multiple sputum cultures. With the deterioration of clinical situation, several other bacteria and fungi were found in sputum culture. Pulmonary infiltrates were evident in right middle and lower lobe at day 5 after illness, and rapidly progressed to involve bilateral lungs as acute respiratory distress syndrome (ARDS)-like changes. The patient was treated with antiviral, antibacterial, and antifungal reagents, and corticosteroids and invasive mechanical ventilation were also administered, but without any improvement. The patient died 27 days after the onset of symptoms and an autopsy was performed. Pathologically, the lungs exhibited diffuse alveolar damage. The lymphocytes in the spleen, the lymph nodes and the tonsils were depleted prominently with histiocytic hyperplasia and hemophagocytic phenomena. Edema and degeneration of myocytes in the heart and extensive acute tubular necrosis in the kidney were observed. CONCLUSION: The prognosis was very poor if influenza A (H5N1) infected human cases was developed as ARDS with multiple organ damage or failure.

Adult↗

PubMed2006

[Impact of acupuncture on NP-1 expression in spared dorsal root ganglion].

OBJECTIVE: To investigate the impact of acupuncture on NP-1 expression in spared dorsal root ganglion (DRG). METHODS: Twenty-five male adult cats were divided into five groups. The five cats in the pseudo-operation group were kept intact. Ten cats were subjected to unilateral root rhizotomy(unilateral L1-L5 DRGs were transected, but L6 DRG was spared), with five sacrificed seven days later and another five sacrificed 14 days later. Ten cats with unilateral root rhizotomy received acupuncture (electron-needle stimulation in Zusanli, Xuanzhong, Futu and Sanyinjiao which are located in the distribution area of L6 spinal nerve) for 30 minutes per day. Five cats in the acupuncture group were sacrificed seven days and 14 days later respectively. The L6 DRG from the experimental side of each animal was taken and made into frozen sections 20 microm in thickness. The sections were stained under the same conditions using NP-1 specific antibody (1:600, Santa) by immunohistochemistry ABC method. The amounts of NP-1 immunoreaction neurons of DRG were measured. RESULTS: The NP-1 immunoreactants were mainly located in the small and medium-sized neurons and a few large sized neurons in the cats of the pseudo-operation group. Less NP-1 positive medium-small sized neurons were found seven days after partial dorsal root rhizotomy (P<0.05). But they returned back to the original level 14 days after operation. The NP-1 positive large neurons had no significant changes after operations. With seven days of acupuncture, the amounts of NP-1 positive small and medium-sized neurons increased significantly (P<0.05) but were still less than the original level (P<0.05). There were no differences in neurons 14 days after operations between the acupuncture and non-acupuncture group (P>0.05) and between the groups with seven days acupuncture and 14 days of acupuncture (P>0.05). CONCLUSION: Acupuncture increases the expression of NP-1 in small and medium-sized neurons of spared DRG.

Acupuncture↗

PubMed2006

[Sequence analysis of a novel HLA-A*3308 allele].

OBJECTIVE: To investigate the molecular genetics basis for HLA novel allele HLA-A*3308 in Chinese population. METHODS: DNA was extracted from whole blood by PEL-FREEZ DNA extraction kit. The amplification of HLA-A exons 1-8 of the proband was preformed by PCR and the amplified product was cloned with TOPO cloning sequencing kit to split the two alleles apart. Both strands of exons 2, 3 and 4 of chosen clones were sequenced. The PCR-SSP was performed to confirm the mutations detected by sequencing. RESULTS: The sequencing results showed HLA-A alleles of the proband as A*0201 and the novel allele. The sequences of the novel allele have been submitted to GenBank (DQ089631, DQ089632, DQ089633). BLAST analysis showed that the novel allele got the difference from A*3303 by five nucleotides at positions 240 A>T, 256C>G, 259A>G, 261C>G and 270T>A in exon 2. This resulted in three amino acids changes from Arg to Gly at codon 62, Asn to Glu at codon 63 and Asn to Lys at codon 66. CONCLUSION: This allele is a novel allele and has been officially named A*3308 by the WHO Nomenclature Committee.

Alleles↗

PubMed2006

[Allelic polymorphism analysis of killer cell immunoglobulin-like receptor gene 3DL2 in Zhejiang Han population].

OBJECTIVE: To analyze the allelic polymorphism of killer cell immunoglobulin (Ig)-like receptor (KIR) gene 3DL2 in Zhejiang Han population. METHODS: Allelic definition within KIR3DL2 was achieved through using the PCR-sequence specific oligonucleotide probes (PCR-SSOP) method and the specific amplification of exon 3-exon 4 and exon 8-exon 9. Twenty-one digoxigenin-labelled probes were used to the SSOP analysis. RESULTS: Seven alleles of KIR3DL2 were observed, in which KIR3DL2 *002 had the highest allele frequency, 0.57. One sample could not be given the allele combination according to the probe profile. CONCLUSION: The PCR-SSOP method was reliable. There is a distinctive allele frequency of KIR3DL2 in Zhejiang Han population.

Asian People↗