Search PubMed⌕ Search

Biomedical subjects

W van Raamsdonk

Publications and source records attributed to W van Raamsdonk.

28 records · Page 2Linked to original sources
PubMed1983

Acetylcholinesterase and acetylcholine receptor histochemistry on end plate regions, myotendinous junctions, and sarcolemma in the axial musculature of three teleost fish species.

In this paper we describe a rapid procedure for the identification of motor end plates in fish. We demonstrated the presence of acetylcholine receptors by means of an immune fluorescence technique with alpha-bungarotoxin. Koelle's thiocholine method was used to localize acetylcholinesterase (AChE) activity. Under carefully controlled conditions the AChE activity and the anti-alpha-bungarotoxin fluorescence showed an equal distribution. This means that in the study of motor innervation in fish the AChE reaction can be used to stain only the motor end plates, leaving the AChE rich preterminal axons unstained. Comparison of the AChE reaction pattern with the distribution of binding sites for antibodies raised against neurofilament protein revealed that in end plate regions high concentrations of AChE are only present in axons and end plates. The myotendinous junctions also possess a high receptor density and enzyme activity. A low enzymatic activity was found at the non-junctional periphery of white muscle fibres. This activity probably resides in the sarcolemma. No non-specific cholinesterase activity was found. From light microscopical analysis it appeared that a single end plate may innervate 2 adjacent muscle fibres. This was affirmed by ultrastructural observations. The dual innervation suggests that, in fish, motor units have a limited distribution through the myotome.

Acetylcholinesterase↗
PubMed1982

Differentiation of muscle fiber types in the teleost Brachydanio rerio, the zebrafish. Posthatching development.

The trunk musculature of adult zebrafishes contains three major fiber types: adult red, intermediate, and white; and two minor populations: red muscle rim and scattered intermediate fibers. In this paper, the post hatching development of these muscle fiber types was studied by means of immunohistochemistry, using anti-myosin sera. Just hatched larvae contain two muscle fiber populations: embryonic red and white, which give rise to the red muscle rim and the intermediate fibers respectively. Adult red fibers arise post hatching as a new separate population with distinct myosin properties. The differentiation of these fiber types occurs within the first four weeks after fertilization, when the adult pattern of peripheral axon bundles has become established. Differences in the muscle fiber type composition between the midbody and the tail myotomes become apparent in two month old fries. The number of scattered intermediate fibers increases from rostral to caudal, the opposite holds for the red muscle rim fibers. The red and intermediate area is triangular in the midbody; in the tail part it is stretched out along the lateral surface of the myotomes. These changes are considered as adaptations to improve the efficiency of the swimming performance.

Animals↗
PubMed1982

Effects of immobilization and partial denervation on the differentiation of muscle fiber types in the Zebrafish, Brachydanio rerio.

In this study we tested the effects of immobilization and of spinal cord lesion on the differentiation of muscle fiber types in the myotomal muscle of the zebrafish. We found that the differentiation of embryonic red and white muscle fibers occurs in immobilized as well as in denervated myotomes of zebrafish embryos. Intermediate and adult red muscle fibers are not formed in denervated myotomes of larvae. Adult zebrafishes with spinal cord lesions in the anal region show abnormal undulating movements in the rostral part of the body. This change in muscle activity correlates with an increase in the number of intermediate scattered fibers. We conclude that direct contact with spinal cord motorneurons is not necessary for the differentiation of embryonic red and white fibers. The differentiation of intermediate muscle fibers is under extrinsic control. The factors that determine the formation of adult red fibers are not yet clear.

Animals↗
PubMed1981

Immunohistochemical differences in myosin composition among intrafusal muscle fibres.

Mammalian intrafusal fibre types (nuclear chain, nuclear bag1 and nuclear bag2 fibres) are known to differ in their ultrastructure, intensity of the myofibrillar histochemical ATP-ase reaction, type of innervation and time course of contraction. The present study concerns the myosin composition of these intrafusal fibre types in the soleus muscle (mouse) and the extensor digitorum longus muscle (rat). We used an immunohistochemical method with three myosin antisera raised in rabbits: anti chicken pectoral myosin, anti chicken heart myosin (1) and anti chicken heart myosin (2) (= anti chicken heart myosin (1) adsorbed with muscle powder from soleus muscle of guinea pig). The results showed that three intrafusal fibre types differed in their myosin composition. A comparison of intrafusal fibre types with extrafusal fibre types for the histochemical myofibrillar ATP-ase reactivity and the reactivity with myosin antisera showed a resemblance of nuclear chain fibres with extrafusal type II fibres and a difference between nuclear bag1 and nuclear bag2 fibres and all other fibre types.

Adenosine Triphosphatases↗
PubMed1980

An immune histochemical and enzymic characterization of the muscle fibres in myotomal muscle of the teleost Brachydanio rerio, Hamilton-Buchanan.

The myotomal muscles of teleosts have a complex internal structure. For a better understanding of the functional anatomy of these muscles we analysed the myofibrillar properties of the myotomal muscle fibres in a small teleost: Brachydanio rerio, the zebrafish. By immune histochemical techniques we discerned 4 types of muscle fibres: red lateral fibres, red muscle rim (RMR) fibres, intermediate fibres and white fibres, each with a distinct type of heavy chain myosin. In addition we discerned 2 populations of muscle fibres in which the myofibrils contain mixtures of white and intermediate heavy chain myosin. The immune histochemical classification correlates well with an enzyme histochemical classification of the muscle fibres. In order to find out whether the immune and enzyme histochemical differences correlate with differences in dynamic properties of the muscle fibres, we analysed the ATP-ase activity of glycerinated muscle fibres. 3 of the 5 muscle fibre types which are discriminated by immune histochemical methods could thus be analysed. Differences in histochemical reactions coincide with differences in ATP-ase activities of the glycerinated muscle fibres. 2 classes of muscle fibres were homogeneous as to the ATP-ase activity of their muscle fibres. The 3rd class, the white fibres, could be divided in 2 populations with slightly different ATP-ase activities. The results indicate that the myotomal muscle fibre population is much more heterogeneous than has been considered thusfar. This heterogeneity may allow an almost continuous range of contraction speeds in the myotomal muscle.

Adenosine Triphosphatases↗
PubMed1980

Myofibrillar differences among mammalian skeletal muscle fibres at the ultrastructural level. A comparison of immunocytochemical and morphometrical parameters.

In the light microscope two types (I, II) of skeletal muscle fibres can be distinguished with antibodies against myosin isozymes. At the ultrastructural level a difference in Z-line width has led to muscle fibre classification. In this study we distinguish at the ultrastructural level between type I and type II fibres of the M. soleus and M. plantaris of adult mice using ultracryosections with immuno-ferritin and antisera against myosin isozymes. Muscle fibres of the M. plantaris are identified as type II fibres and the fibres of the M. soleus are divided in type I and type II fibres. In the immunologically identified fibres the filament overlap in the Z-line was measured. The type II fibres of the M. plantaris have narrow Z-lines, whereas type I and type II fibres of the M. soleus have wide Z-lines. We conclude that a classification of fibres based on Z-line width differs from the type I/type II classification. The antimyosin antibodies react exclusively with the A-band. In serial sections the myosin isozymes can be identified unambiguously. This is a prerequisite for further studies of myosin isozyme distribution in "mixed" muscle fibres.

Animals↗
PubMed1979

Differentiation of the musculature of the teleost Brachydanio rerio. II. Effects of immobilization on the shape and structure of somites.

The development of the shape and structure of somites in the teleost Brachydanio rerio was studied in embryos under normal conditions and in immobilized embryos. Three different immobilization methods were applied: enclosure in agar, a glass rod in the neural tube and anaesthesia in MS-222. When the performance of the lateral body movements is prevented, the shape development of the somites in embryos and young larvae becomes reversed. When the agar-immobilization is terminated, the larvae resume their normal movements. In about 10 days, the shape of the somites is again as in control larvae. We conclude, that the lateral body movements have both a shape-determining and a shape-stabilizing role during the early stages of somite morphogenesis. It is suggested that in normal embryos differences in shortening between lateral and medial muscle fibres, cause differences in longitudinal growth of the muscle fibres and that the oblique muscle fibre arrangement is a consequence of these differences in growth. In immobilized embryos and larvae, the longitudinal growth of the muscle fibres is decreased. Also the difference in the longitudinal growth rate between lateral and medial muscle fibres diminishes in all somites. We conclude that for the normal morphogenesis of the somites the performance of the specific function, that is to bring about lateral body movements, is required. We suggest, that the impact of the lateral body movements on the development of the structure of the somites is mediated through adaptive growth of the muscle fibres. The suggestion may also apply to the development of the pinnate structure of muscles of higher vertebrates.

Agar↗
PubMed1977

Detection of antigens and antibodies by an immuno-peroxidase method applied on thin longitudinal sections of SDS-polyacrylamide gels.

This paper describes a method for detection of antigens in thin sections of SDS-polyacrylamide gels. This method, called SGIP, involves longitudinal sectioning of SDS gels, fixation of the proteins in the gels, removal of the SDS, incubation of the sections with an antiserum and detection of antigens by the indirect immuno-peroxidase technique. The method is useful for assessing the affinity spectrum of a given antiserum against a heterogeneous mixture of proteins, and for the detection of proteins in tissue homogenates or other protein mixtures by means of well defined antisera. By applying the method to serial sections from a single SDS-polyacrylamide gel, a dilution dependent reactivity of antisera is demonstrated.

Acrylamides↗