Search PubMed⌕ Search

Biomedical subjects

W Zimmermann

Publications and source records attributed to W Zimmermann.

At least 181 records · Page 10Linked to original sources
PubMed1986

Studies on the SV40-like papovavirus SV40-GBM. III. Propagation at low multiplicities of infection in various human cell lines.

At low multiplicity several human cell lines supported the lytic infection with SV40-GBM better than that with the wild type SV40. The efficiency of viral DNA replication differed in the cell lines used suggesting that specific host cell factors may determine the rate of viral DNA synthesis. Furthermore, the emergence of different DNA defects during propagation of the virus indicates that host cell factors in question might also influence the composition of the viral DNA population.

Cell Line↗
PubMed1986

[Further development and restandardization of an approach to the diagnosis of aggressive social behavior in childhood].

The present paper continues and concludes an investigation into the restandardization of a first attempt, checked in respect of content and method, at the diagnosis of aggressive social behavior initially concentrating on the 9-11 age group. The work has its origin in a paper by Petermann and Petermann (1980), which we consider could be of significance to gradual restandardization, both in methodological research and in practical clinical application. A previous article demonstrated under various aspects the fundamental suitability of the attempt to restandardization. Arising out of this and the present analysis, some fundamental consequences, both in content and in method, of a fuller development and the therapy oriented employment of the attempt are discussed.

Aggression↗
PubMed1986

[Differential diagnostic value of the Diagnostic Test for Cerebral Damage in clinical practice].

The paper considers the effective inclusion in differential diagnosis of DCS in decisions involving groups of patients not yet primarily exposed to the process. 55 patients were involved in two samples taken within the group as a whole. One of the samples is characterised as being unambiguously diagnosed neurotic without there being any neurologic-psychiatric or other (internal medical) findings (no organic brain injury, no symptoms of seizure, no cerebro-vascular insufficiency CVI, etc.). The other sample comprehended patients with a clear CVI diagnosis, minor EEG findings, and with or without internal medical findings. By applying a specific "index of instability" resulted in an assured diagnostic distinction.

Adult↗
PubMed1984

[Preventive chemotherapy of intrapulmonary residual tuberculosis with isoniazid--example of failed compliance by the physician].

Compliance with the recommendations of the Swiss Society for Tuberculosis and Lung Diseases regarding the treatment of patients with fibrotic pulmonary lesions with isoniazid was evaluated over the period March 1981-October 1982. 42 patients (mean age 53 +/- 16 years) were selected for preventive treatment. Diagnostic procedures were correctly performed in 22 patients (52.4%). 32 patients (76.2%) received the recommended drug initially. In 18 patients (42.9%) isoniazid was correctly prescribed for six months. In only 15 patients (35.7%) was the diagnostic and therapeutic approach correct. The reasons for these quality inadequacies on the diagnostic and therapeutic levels are discussed.

Aged↗
PubMed1984

Antibiotic action on phagocytosed bacteria measured by a new method for determining viable bacteria.

Antibiotics such as rifampin that act on phagocytosed bacteria have clinical advantages. The investigation of such intraphagocytic activity of antibiotics is, however, hampered by the time-consuming and cumbersome procedures necessary for enumerating viable bacteria. We have developed a semiautomatic biophotometric method for this purpose that permits the processing of relatively large numbers of samples. Using this method with Staphyloccus aureus Wood 46 as a test organism, we studied the dose dependence of the activities of a number of antibiotics against intraphagocytic bacteria. We could confirm the very good intracellular activity of rifampin. Its activity at low concentrations was slightly better against intracellular than against extracellular bacteria. In contrast, clindamycin and erythromycin, both of which have been reported to accumulate within phagocytes, did not exhibit a correspondingly enhanced intracellular activity, erythromycin being active intracellularly only at high concentrations. Clindamycin and vancomycin were effective against intracellular organisms, but only at relatively high concentrations owing to their low bactericidal activity against S. aureus Wood 46. Penicillin G, ampicillin, gentamicin, and streptomycin exhibited no useful intracellular activity. These results demonstrate that radioactive accumulation studies of antibacterial agents are not sufficient to show intracellular activity. Intracellular activity must be demonstrated in a functional test, preferably with graded concentrations of the test substance.

Anti-Bacterial Agents↗
PubMed1984

Molecular analysis of the envelope gene and long terminal repeat of Friend mink cell focus-inducing virus: implications for the functions of these sequences.

We sequenced the envelope (env) gene and 3' long terminal repeat of a Friend mink cell focus-inducing virus (F-MCFV). We also sequenced the gp70 coding regions for two cDNA clones of another F-MCFV. The deduced amino acid sequence of the env gene products of both F-MCFVs were compared to the corresponding sequences of other MCFVs and of ecotropic viruses. The env polypeptides of the different viruses showed long stretches of homology in the carboxy-terminal half of gp70 and in p15env ("constant region"). The amino-terminal half of gp70 was very similar in all MCFVs, but showed extensive variations relative to the ecotropic viruses ("differential region"). This differential region in all MCFVs is of endogeneous origin. We show evidence that this region carries determinants for ecotropic or polytropic host range. No indication could be found that the env gene products determine the histological type of disease caused by particular MCFVs. When the long terminal repeats of F-MCFV and Friend murine leukemia virus were compared with those of other viruses causing either lymphatic leukemia or erythroleukemia, several nucleotides were localized which might determine the histological type of disease caused by these viruses.

Amino Acid Sequence↗
PubMed1984

Characterization of the DNA of the hamster papovavirus: I. Genom length and molecular cloning.

The complete genome of the hamster papovavirus (HaPV) which was isolated from virions found in multiple skin tumors of the Syrian hamsters was measured by electron microscopy and cloned in Escherichia coli using the certified plasmid vector pBR322. The cloned viral DNA were characterized by digestion of the recombinant DNA with various restriction enzymes followed by comparison of their electrophoretic mobilities in agarose gels with that of similarly digested uncloned DNA and by electron microscopy to determine the genome size of cloned HaPV DNA. The restriction enzyme analysis of the cloned HaPV DNA showed the same cleavage pattern as the corresponding fragments from the uncloned DNA. No major insertions or deletions could be detected by heteroduplex analysis between cloned HaPV DNA and the starting material. The estimated genome size of 5.52 kb for HaPV DNA is approx. 300 bases larger than those determined for other known papovaviruses as SV40 or polyoma.

Animals↗
PubMed1983

Characterization of a prokaryotic topoisomerase I activity in chloroplast extracts from spinach.

A topoisomerase I activity has been partially purified from crude extracts of spinach chloroplasts. This activity relaxes the supercoiled covalently closed circular DNA of pBR322. The enzyme requires Mg++, but not ATP, and has an apparent molecular weight of about 115,000. It catalyzes a unit change in the linkage number of supercoiled DNA but cannot relax positive supercoiled DNA. These characteristics of the topoisomerase suggest it is of the prokaryotic type and would tend to support the endosymbiotic theory of plastid origin and evolution.

Chloroplasts↗