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Biomedical subjects

W Zhao

Publications and source records attributed to W Zhao.

388 records · Page 22Linked to original sources
PubMed1990

Hb Hekinan observed in three Chinese from Macau; identification of the GAG----GAT mutation in the alpha 1-globin gene.

Hb Hekinan, an alpha chain variant that is characterized by a Glu----Asp mutation at position alpha 27, was observed in three Chinese females attending a prenatal clinic in Macau. The relative quantities of the stable hemoglobin were 13-14% (average 13.3%); its identification was greatly aided by the separation and purification of the peptides by reversed phase high performance liquid chromatography. Dot-blot analysis of amplified DNA with 32P-labeled probes located the mutation in codon 27 of the minor alpha 1-globin gene; the change involved a GAG (coding for glutamic acid) to GAT (coding for aspartic acid) mutation.

Adult↗
PubMed1990

[Observation for 3 years on the immune effects of hepatitis B vaccine in susceptible population].

Chinese-produced HB vaccine induced high levels of antibodies against HB surface antigen in susceptible population (babies born to mothers who were negative for HBsAg and people who were negative for all the HBV markers). Eight months after the first dose, 97.73 and 93.75 percent of the neonates who received 10 micrograms (group I) and 20 micrograms (group II) vaccines and 92.45 percent of the adults who received 20 micrograms vaccine (group III) developed anti-HBs with the S/N values of 42.12, 46.53 and 36.20, and gave effective protection rates (S/N greater than or equal to 10) of 86.36%, 89.58% and 79.25%, respectively. No significant difference was noted between these groups. After three years, the positive rates and the S/N values of anti-HBs were 17.1% and 3.70 in group I, 15.6% and 4.23 in group II, 20.4% and 4.04 in group III respectively. One month after booster injection in anti-HBs, the S/N values of anti-HBs were 85.12 in group I with 10 micrograms vaccine, 89.23 in group II with 30 micrograms vaccine and 90.82 in those aged 4-10 years with 10 micrograms vaccine. The infection rate in the neonates who were not vaccinated with HB vaccines (11.11%) was higher than those who were vaccinated (0%). So it is clear that Chinese-produced HB vaccines are safe and effective.

Adult↗
PubMed1990

[Effects of alcohol extract from Polygonatum odoratum (Mill.) Druce and Cuscuta australis R. Br. on immunological function of mice injured by burns].

This paper shows that the alcoholic extract from Polygonatum odoratum and Cuscuta australis increases the percentage of phagocytosis and the index of phagocytosis of macrophages as well as hemolysin and proliferation of lymphocytes up to normal level in mice with II+ burn injury covering 5% body surface. It follows that both Polygonatum odoratum and Cuscuta australis may serve as a kind of immunopotentiator for mammals.

Adjuvants, Immunologic↗
PubMed1988

The relationship between Hb Bart's levels in cord blood and the deletions of alpha-globin genes.

A relationship between Hb Bart's levels in cord blood and the number of alpha-globin genes has been established by screening cord blood samples from 1,075 newborn babies. Diagnosis was made by gene mapping of DNA samples with restriction endonuclease digestion. The presence of Hb Bart's was determined with a discontinuous microelectrophoresis on cellulose acetate at pH 8.34. The establishment of this relationship allows an early diagnosis of alpha-thalassemia by this simple microelectrophoretic procedure.

Chromosome Deletion↗
PubMedDate not supplied

Production of extracellular protease from crude substrates with dregs in an external-loop airlift bioreactor with lower ratio of height to diameter.

Bacillus subtilis AS1.398 was cultivated in a 11.5-L total volume external-loop airlift bioreactor with a low height-to-diameter ratio of 2.9 and a riser-to-downcomer diameter ratio of 6.6 for the production of protease from crude substrates with dregs. The influence of aeration rate, liquid volume, and sparger hole diameter on protease production was investigated. An average of 8197 u/mL protease activity was obtained after a total fermentation time of 32 h in the external-loop airlift bioreactor with a liquid volume of 8.5 L, air flow rate of 1.5 vvm, and sparger hole diameter of 1.5 mm. The addition of one stainless steel sieve plate in the riser of the airlift bioreactor increased productivity of protease. After 32 h of fermentation, an average of 8718 u/mL protease activity was achieved in the external-loop airlift bioreactor with one sieve plate and an air flow rate of 1.2 vvm, liquid volume of 8.5 L, and gas sparger hole diameter of 1.5 mm. This was 9.0% higher than the typical averages of about 8000 u/mL protease activity in the mechanically stirred tank bioreactors of the enzyme factory using the same microorganism. It is possible to make a scale-up of the external-loop airlift bioreactor and feasible to operate it for production of protease from crude substrate with dregs.

Bacillus subtilis↗
PubMedDate not supplied

Bone regrowth after digit tip amputation in mice is equivalent in adults and neonates.

Despite clinical and experimental reports of digit tip regrowth, bone regrowth after amputation through terminal phalanges has not been methodically documented. We have examined bone regrowth in mice after amputation through the terminal phalanx to determine how the level of amputation affects the response and whether the response varies between adults and neonates. Digit tips were amputated, and, at selected intervals greater than 5 weeks after amputation, digits were photographed and processed for whole mount staining with alizarin red stain. Amputations within the distal 40% of the terminal phalanx yielded grossly normal digits within 5 weeks and bone regrowth which usually attained or surpassed the original length. Amputations through the proximal 20% of the distal phalanx precluded nail plate regrowth, and bone grew minimally or regressed. Amputation through the intermediate 40% of the distal phalanx produced digit tips in which bone regrowth was correlated with nail regrowth and in which bone regrowth did not reach original levels. The response in adults and neonates was similar. The correlation between nail regrowth and bone regrowth may help to predict phalangeal bone regrowth after amputation in adults and juveniles.

Journal Article↗
PubMedDate not supplied

Flat-panel digital radiology with amorphous selenium and active-matrix readout.

Future imaging techniques will be capable of high image quality, fast acquisition, compactness, and versatile operation. A flat-panel imager that is expected to achieve these goals is under development. It consists of a thin layer of amorphous selenium that converts x rays directly to an electric charge and a thin-film electronic circuit, or active matrix, to read out the electronic signal directly to a computer host. The advantages of amorphous selenium include high resolution and low noise without loss of signal strength. The advantages of the active matrix are real-time readout, flexible design parameters, and compactness of the readout structures. A prototype of this imager has been built and operated, and initial images (of an x-ray test bar pattern and a hand phantom) have been acquired. Although the prototype was built to test scientific principles and many possibilities for optimization remain, the images already possess the quality necessary for many radiographic procedures. A large range of current and new applications exist for this imager.

Hand↗