Biomedical subjects

W Zhang

Publications and source records attributed to W Zhang.

At least 523 records · Page 29Linked to original sources

PubMed2000

[Variable number of tandem repeats as an evidence of engraftment status after allogeneic peripheral blood stem cell transplantation].

OBJECTIVE: To evaluate engraftment status of patients after allogeneic peripheral blood stem cell transplantation (allo-PBSCT) based on red blood cell type, karyotype and variable number of tandem repeats (VNTR). METHODS: Amplification of 3 loci of VNTR in vitro by polymerase chain reaction (PCR) was preformed to detect the polymorphism of 15 patients post-PBSCT. Furthermore, analysis of karyotype in 7 sex-mismatched patients and red blood cell antigen switching in 3 ABO blood type mismatched patients were also employed. RESULTS: VNTR analysis in 15 patients revealed complete chimerism in 11 patients and mixed chimerism in 4. In the 7 sex-mismatched patients, karyotype analysis showed types of their donors. As for blood type analysis, it was after 2 months that the first patient transformed to his donor's type. One patient showed mixed status 7 months after transplantation. CONCLUSION: Amplification of VNTR loci by PCR for documenting engraftment status after allo-PBSCT is simple and sensitive. This technology is especially suitable for patients who have received stem cells with the same sex, same blood type and identical HLA type. Examination with several different VNTR loci will improve the power of discrimination.

Adolescent↗

PubMed2000

[Treatment of intrauterine growth retardation with magnesium sulfate].

OBJECTIVE: To study the relationship between magnesium level in umbilical vein and mother's peripheral blood with intrauterine growth retardation (IUGR) and fetal weight. METHODS: 39 pregnant women with IUGR were randomly divided into 3 groups: Group 1 (n = 14): The patients were treated with 10% glucose 500 ml + danshen compound 14 ml + low molecular weight dextran 500 ml i.v.; Group 2 (n = 14): in addition to the same treatment as in group 1, 25% magnesium sulfate 20 ml in 5% glucose 500 ml i.v. was given; Group 3 (n = 11): no treatment was given; and another 12 normal term delivery women were served as control. Magnesium concentration levels were determined in both maternal peripheral blood and their fetal umbilical vein. RESULTS: Maternal serum magnesium level was higher in Group 2 (1.06 +/- 0.09) mmol/L than that in Group 1 (0.69 +/- 0.05) mmol/L (P < 0.05), while there was no significant difference between Group 1 and Group 3. The serum magnesium levels in both mother and fetal umbilical vein of normal pregnancy and of Group 2 were significantly higher than those of Group 1 and 3 (P < 0.01), so did the birth weight of their newborns (P < 0.05). CONCLUSION: Magnesium deficiency might be one of the causes of IUGR, and magnesium sulfate treatment was effective.

Adult↗

PubMed2000

[Prognostic factors in granulosa cell tumor of the ovary].

OBJECTIVE: To study the effect of clinical and pathologic factors on prognosis for granulosa cell tumor of the ovary. METHODS: The clinical records and tumor sections of 100 patients with granulosa cell tumors of the ovary between 1958 and 1995 were reviewed. The relationship between age, stage, mitosis and the adjuvant therapy for early stage (stage I) were analyzed retrospectively. Patients with recurrent tumors were compared with patients who remained without disease, patients with early recurring tumors were compared with late recurring tumors. RESULTS: The overall 5 and 10 year survival rates were 80% and 72%. There were no significant difference between ages and survival (P > 0.05). The survival rates in stage I were 98% and 96% after 5 and 10 years, respectively, and in stages II were 70% and 60%, after 5 and 10 years, all of 4 patients with stage III-IV were dead of recurrent disease in 1 year. The frequency of observed mitosis influenced the survival rate: with less 5/10 high-power fields (HPF) the survival were 96% both at 5 and 10 years, with more or equal 5/10 HPF the 5 and 10 year survival rates were 58% and 36%, respectively (P < 0.01). No significant correlation could be established between the adjuvant therapy and 5 and 10 years survival in 56 stage I patients. There were 44 patients with recurrent disease in this group, median time to recurrence was 53 months. Late recurrence appeared in 8 cases. The significant differences in stage and abdominal mass were noted between the recurrent tumors and the group without disease (P < 0.05). When early and late recurring tumors were compared, statistically significant differences were again noted: early recurring tumors had higher mitotic rates and late stage, and late recurring tumors had lower mitotic rages and early stage. Patients without recurrent tumors were similar to the patients with early recurring tumors. CONCLUSIONS: Tumor stage and mitotic rate are the clinical and pathologic prognosticators in granulosa cell tumor. It is difficult to predict late recurrences using these clinical and pathologic parameters.

Adolescent↗

PubMed2000

[The promoting effects of mGM-CSF gene modified tumor vaccine immunization on peritoneal antigen presenting cells in mice].

OBJECTIVE: To investigate the effects of immunization with granulocyte-macrophage colony-stimulating factor (GM-CSF) gene-modified tumor vaccine on the number and function of antigen presenting cells (APCs). METHODS: FBL-3 murine erythroleukemia cells were transfected with mGM-CSF recombinant adenovirus and then irradiated to prepare vaccine. The specific cytotoxic T lymphocyte(CTL) activity in vivo and survival of the mice i.p. immunized with this vaccine were observed. The percentage of 33D1+ dendritic cells(DCs) in peritoneal adherent cells were analyzed with flow cytometry. The number of peritoneal cell and function of peritoneal adherent cells of immunized mice were also investigated. RESULTS: High level of specific CTL activity were induced in the mice i.p. immunized with this vaccine and all of the mice were resistant to the subsequent challenge with the parental FBL-3 cells. The number of peritoneal APCs-increased. FACS analysis showed that the percentage of 33D1+ DCs in peritoneal adherent cells also increased. The allo-mixed lymphocyte reaction(MLR) stimulating activity of these APCs was enhanced. CONCLUSION: mGM-CSF gene-modified tumor vaccine has potent anti-tumor effect, and this may be related to the increased number and function of peritoneal APCs induced by mGM-CSF through paracrine mechanism at the vaccination site.

Animals↗

PubMed2000

Changes in soluble interleukin-2 receptor level in serum and Na+-K+-exchanging ATPase activity in semen of infertile men caused by antisperm antibody.

AIM: To explore the possible mechanisms of male infertility caused by antisperm antibody (AsAb). METHODS: The soluble interleukin-2 receptor (sIL-2R) level in serum was analyzed by ELISA and Na+-K+-exchanging ATPase activity in semen by phosphorus (Pi) assay. RESULTS: The sIL-2R level in serum was significantly higher and the Na+K+-exchanging ATPase activity in semen significantly lower in AsAb positive infertile men when compared with the controls. CONCLUSION: The AsAb titer varies with the sIL-2R level in serum. A decrease in Na+-K+-exchanging ATPase activity in semen may play a role in male infertility caused by AsAb.

Adult↗

PubMed2000

[Expression of beta 2-adrenoceptor on peripheral lymphocyte and the level of plasma cAMP in patients with pregnancy induced hypertension].

OBJECTIVE: To evaluate the alteration of beta 2-adrenoceptor-cAMP system in patients with pregnancy-induced hypertension (PIH). METHODS: The Bmax of beta 2-adrenoceptor on the lymphocyte was examined by radioligand binding technique. The level of plasma cAMP was observed by radio competitive protein binding assay. RESULTS: The Bmax of beta 2-adrenoceptor (259 fmol/10(6) cell +/- 22 fmol/10(6) cell) on the lymphocytes was significantly higher in PIH group than in control group (247 fmol/10(6) cell +/- 23 fmol/10(6) cell). There was no significant difference between the PIH group and the controls. The content of plasma cAMP (2.94 nmol/40 microliters +/- 0.91 nmol/40 microliters) was significantly higher in PIH group than in control group (0.19 nmol/40 microliters +/- 0.05 nmol/40 microliters). CONCLUSION: The mechanism of PIH may be related with the activity of beta 2-adrenoceptor-cAMP system.

Adult↗

PubMed2000

Macrophage activation of lymphoma-bearing mice by liposome-mediated intraperitoneal IL-2 and IL-6 gene therapy.

OBJECTIVE: To investigate the antitumor mechanism of interleukin-2 (IL-2) and interleukin-6 (IL-6) gene therapy. METHODS: Liposome encapsulated IL-2 DNA and IL-6 DNA were intraperitoneally (i.p.) injected into mouse lymphoma cell line (EL-4) lymphoma-bearing mice. Macrophage function (M phi) from the mice was assessed. RESULTS: Cytotoxicity, major histocompatibility (MHC) II expression and IL-1 and TNF secretion of the macrophages all augmented after i.p. injection of liposome encapsulated IL-2 DNA or IL-6 DNA. More efficient activation of macrophages was observed in mice treated with liposome encapsulated IL-2 DNA than IL-6 DNA. IL-2 gene therapy combined with IL-6 gene therapy showed the maximal activation of macrophages in the lymphoma-bearing mice. CONCLUSION: IL-2 and IL-6 gene therapy can relieve the suppression of macrophages of the lymphoma-bearing mice, and efficiently activate the antitumor immune responses.

Animals↗

PubMed2000

Molecular and immunohistochemical study of the inactivation of the p16 gene in primary hepatocellular carcinoma.

OBJECTIVE: To determine whether p16 gene is involved in the genesis of primary hepatocellular carcinoma (HCC). METHODS: Twenty-five HCC tumor samples with corresponding non-tumor liver tissue specimens were examined for p16 gene alterations. The identification of deletion of exon 1 and exon 2 in p16 gene was performed using comparative multiplex polymerase chain reaction (PCR) analysis. The point mutation of exon 2 in p16 gene was investigated by single strand conformational polymorphism (SSCP) analysis, and the status of p16 gene methylation was screened using a PCR based methylation analysis. 35 parafin-embedded specimens of HCC with corresponding non-tumor liver tissues, including the 25 cases described above for screening p16 gene alterations, were investigated for p16 protein expression using immunohistochemical analysis. RESULTS: Among 25 cases, 2 homozygous deletions and 1 hemizygous deletion were found in HCC samples. No point mutation was identified in the remaining 22 tumor samples without p16 gene deletions. Hypermethylation was detected in 24% (6/25) of tumor samples. However, the corresponding non-tumor liver tissue specimens were always unmethylated at the p16 locus. Loss of p16 protein expression occurred in 16 of 35 (45.7%) tumor samples, and all the non-tumor liver tissue specimens showed positive p16 staining. For the 25 cases examined for p16 gene alterations, the loss of p16 protein expression was observed in all tumors with p16 gene alterations and also in 3 tumors without p16 gene alterations. CONCLUSION: Inactivation of the p16 gene may play an important role in the genesis of primary hepatocellular carcinoma.

Carcinoma, Hepatocellular↗

PubMed2000

CT virtual bronchoscopy: imaging method and clinical application.

OBJECTIVE: To evaluate the imaging method and clinical application of CT virtual bronchoscopy (CTVB). METHODS: Fifty-two patients with bronchial and pulmonary diseases were studied with CTVB. All patients underwent fiberoptic bronchoscopy and patients with lung cancers were confirmed pathologically. Lung cancer of center type was found in 46 patients, postoperative lung cancer in 4, tracheal adenoidcystic carcinoma in 1, and bronchial diverticulum in 1. CTVB was performed using Navigator Smooth software on the workstation (Advantage Windows 3.1, GE Medical Systems). Source images included slice thickness of 3 mm or 5 mm, pitch of 1.0 or 1.5, 1.0 mm or 2.0 mm interval (overlap more than 60%) reconstruction. RESULTS: CTVB could reveal vividly the tracheobronchial lumens, the cartilage rings, the carina and the left and right main bronchi, down to the fourth order of bronchial orifices, mimicing fiberoptic bronchoscopy. Among 46 patients with lung cancers of center type, fiberoptic bronchoscopy showed the masses in 45 patients and CTVB displayed the masses in 42. The sensitivity of CTVB was 93.3% and its accuracy was 93.5% (chi 2 = 1.33, 0.10 < P < 0.25). The tumors appeared as masses or nodules, causing bronchial stenosis (n = 35) or occlusion (n = 7). The bronchial rings near the masses were blurred, smooth or absent in contrast to the findings of fiberoptic endoscopy. Postoperative bronchial stump (n = 4) appeared to be smooth. Bronchial diverticulum exhibited a local concavity on CTVB and local protrusion on surface shadow display (SSD). CTVB could pass through the stenotic bronchi and detect the occlusive bronchi from the distal end. CONCLUSIONS: The sensitivity of CTVB in detecting bronchial masses was higher than that of fiberoptic bronchoscopy. Combined with multiplanar reconstruction (MPR) and CTVB can demonstrate the extraluminal extension of tumors. As a noninvasive examining method, however, CTVB is limited to observe mucosal abnormalities and to obtain histologic samples.

Adult↗

PubMed2000

[Combination chemotherapy of uterine sarcomas].

OBJECTIVE: To study the results of combination chemotherapy of uterine sarcoma after operation and recurrent tumor. METHODS: One hundred seventy-four cases of three major pathological subtypes of uterine sarcomas were treated in the Cancer Hospital from 1960 to 1996. Clinical data were analyzed of 51 cases of uterine sarcomas treated with postoperative adjuvant chemotherapy and 38 cases with recurrent tumors received 98 courses of chemotherapy. They were divided into 4 groups according to the adjuvant chemotherapy regimen: single drug, VAC, VAD, and other regimens. Chemotherapy regimens for recurrent tumors were VAD, PA/PAC, and other combination regimens including etoposide, ifosfamide, cisplatin, adriamycin. RESULTS: The 5-year survival rate of stage I-II uterine sarcoma patients was 54.9% receiving adjuvant chemotherapy. It was 72.7% in VAD group which was significantly higher than that in other regimen groups. The survival rate was related to the number of chemotherapy course. The chemo-sensitivity of various pathological types of recurrent uterine sarcomas was not different. CONCLUSION: The 5-year survival rate does not improve in patients with stage I-II uterine sarcomas given postoperative chemotherapy. VAD is among the best regimens and at least 3 courses should be performed. The results of new treatment regimens such as EPA, IA, etc., must await further clinical observation.

Antineoplastic Combined Chemotherapy Protocols↗

PubMed2000

[Construction of adenovirus vector for human p27kip1 gene and its expression].

OBJECTIVE: To study the effects of exogenous p27kip1 gene on cell cycle and proliferation. METHODS: E1 substitutive adenovirus vector pAxlcw containing human p27kip1 cDNA (Cihp27kip1) under the transcriptional control of CMV promoter was constructed and then cotransfected with Ad5 adenovirus DNA-terminal peptide complex EcoT221-digested into 293 cells for preparing p27kip1 recombinant adenovirus Adhp27kip1. After infecting HeLa cells for 24 h, the exogenous p27kip1 protein expression in HeLa cells and its effects on cell cycle and proliferation were determined by Western blot, FACS and MTT assay. RESULTS: The titers of resultant recombinant adenoviruses was 1.7 x 10(9) pfu/ml. p27kip1 protein was over-expressed in HeLa cells infected with p27kip1 recombinant adenovirus for 24 h, resulting in blockage of cell cycle transition from G1 phase to S phase and inhibition of cell proliferation. CONCLUSION: p27kip1 recombinant adenoviruses prepared in this study can efficiently transfer p27kip1 gene into HeLa cells and over-express p27kip1 protein in the infected cells. Adhp27kip1 can be used as an agent for gene therapy.

Adenoviridae↗

PubMed2000

[Clinical implications of telomerase activity in breast cancer fine-needle aspirates].

OBJECTIVE: To study the telomerase activity in samples of breast fine-needle aspirates, its clinical implications, and possible association with expression of PR, ER, PCNA, c-erbB2 and p53. METHODS: Ninetynine fine needle aspirates from breast cancers in 83 patients, 12 from benign lesions and 4 from inflammation of the breast, were examined for telomerase activity by a PCR-based telomere repeat amplification protocol (PCR-TRAP). In 35 breast cancer tissue specimens, expression of PR, ER, PCNA, c-erbB2 and p53 were examined by immunohistochemical staining. RESULTS: Among 83 fine-needle aspirates from breast cancer, telomerase activity was detected in 61 of 69 cytology-possitive specimens and in 5 of 7 cytology-suspicious specimens and in 4 of 7 cytology-negative specimens, with an overall positive rate of 84.3% (70/83). The concordance rate of telomerase detection and cytologic examination was 77.1% (64/83). Of 12 fine-needle aspirates from patients with benign breast lesions, 4 were telomerase positive, but 4 aspirates from inflammed breast were all negative. Telomerase activity of breast cancer aspirates did not correlate with histologic type, lymph node status, tumor size and clinical stage, nor did it correlate with PR, ER status and PCNA and c-erbB2 expression. However, negative correlation was found between telomerase activity and accumulation of p53 proteins. CONCLUSION: Detection of telomerase in breast fine-needle aspirates complementary to cytological examination is useful in the diagnosis of breast cancer. Its possible association with p53 accummulation needs further study.

Biopsy, Needle↗

PubMed2000

[Experimental study of effect of meniscus suture on meniscus healing].

OBJECTIVE: To investigate the effect of meniscus suture on meniscus healing which included healing time and healing pattern. METHODS: Fourty healthy rabbits were adopted in this study. The model of meniscus injury was made by a longitudinal incision at the medial meniscus of the left knee. The rabbits were divided into two groups, the experimental group was treated by meniscus suture and the control group was unsutured. After operation, the meniscus samples were collected periodically and observed by gross, light and electronic microscope to analysis the meniscus healing. RESULTS: The injured meniscus was healed gradually and completely at the sixth week in the experimental groups. More fibroblasts and less fibrocartilage cells could be observed in the healed meniscus. Oppositely, there was no meniscus healing in the control group and the edge of injured meniscus was sealed by epithelioid cells. CONCLUSION: The meniscus suture can accelerate the healing process of meniscus injury. Besides, early suture make the injured meniscus correctly positioned to ensure the normal healing process.

Animals↗

PubMed2000

[Clinical study on chitosan for prevention of elbow adhesion].

OBJECTIVE: To study the clinical effect of chitosan on prevention of elbow adhesion after elbow arthrolysis. METHODS: Twenty six patients with elbow ankylosis were performed elbow arthrolysis, which divided into two groups, in chitosan group, 12 patients were injected 2% chitosan into the elbow joint cavity, and no chitosan used in the other 14 patients as control group. The average range of extension and flexion of elbow joint was detected to evaluate the clinical results. RESULTS: All patients were followed up 8 to 51 months, averaged 24 months. In the chitosan group, the average range of extension and flexion of elbow joint was restored to 92.9 degrees +/- 20.9 degrees, with an average increase of 55.0 degrees +/- 15.9 degrees compared with preoperation. In the control group, the average range of extension and flexion of elbow joint was restored to 75.4 degrees +/- 17.5 degrees, with an average increase of 38.2 degrees +/- 11.9 degrees. The outcome showed significant difference between the chitosan group and the control group (P < 0.01). CONCLUSION: Chitosan can prevent or reduce elbow adhesion after elbow arthrolysis.

Adolescent↗

PubMed2000

[Expression of GM-CSE mRNA in asthmatic patients].

OBJECTIVE: To explore non-invasive and specific markers for airway inflammation. METHODS: RT-PCR methods were used to measure GM-CSF mRNA expression in airway mucosa and induced sputum from asthmatic patients. The correlation was analyzed between expression of GM-CSF mRNA and eosinophils or other inflammatory cells. RESULTS: (1) The numbers of eosinophils and EG2 positive cells were (17.9 +/- 7.0) x 10(2) per mm2, and (8.9 +/- 3.0) x 10(2) per mm2 in the asthmatic group, significantly higher than those in the non-asthmatic control which were (1.9 +/- 1.0) x 10(2) per mm2, and (0.8 +/- 0.2) x 10(2) per mm2, respectively (P < 0.05). (2) The expression of GM-CSF mRNA was (1.4 +/- 0.6) in the asthmatic group, significantly higher than that in the control group (0.3 +/- 0.3) (P < 0.05), and GM-CSF mRNA expression was correlated with the numbers of EG2 positive cell (r = 0.73 and P < 0.05). (3) The percentage of eosinophils in induced sputum from asthmatic patients was (0.334 +/- 0.067), which was significantly higher than that from chronic bronchitis and control (0.021 +/- 0.004), (0.008 +/- 0.003) (P < 0.05). (4) The expression of GM-CSF mRNA in asthmatics was (0.320 +/- 0.054), significantly higher than that in chronic bronchitis and the control group (0.188 +/- 0.024 and 0.058 +/- 0.028) (P < 0.05). The expression of GM-CSF mRNA in patients with chronic bronchitis was also significantly higher than that in the control group (P < 0.05). CONCLUSIONS: The expression of GM-CSF mRNA in induced sputum may be a relatively specific marker for airway inflammation.

Adult↗

PubMed2000

[Effect of Weikangfu granule on ultrastructure of gastric mucosa in patients of precancerosis with spleen deficiency syndrome].

OBJECTIVE: To observe the effect of Weikangfu Granule (WKFG) on the ultrastructure of gastric mucosa. METHODS: WKFG was used to treat 61 patients with gastric mucosa intestinal metaplasia or atypical proliferation, and histopathological and ultrastructural examination on gastric mucosa of antral region obtained through gastroscopy were conducted before and after treatment. RESULTS: After treatment, the histopathology and intestinal metaplasia subset of antral focal region, and the background lesion in ultrastructure of the mucosa in the nonfocal region were all improved in the 4 groups, The effects of Spleen-Qi Deficiency syndrome group and Spleen-Yang Deficiency group were superior to Ying-Deficiency group as well as Spleen-Deficiency and Qi-Stagnation group (P < 0.05, P < 0.01), and approached to those in the healthy control group. CONCLUSION: WKFG improves the quantitative balance of internal bioactive substances to maintain the homeostasis, promotes the ultrastructural normalization of mucosa so as to cure the clinical symptoms and reverse the intestinal metaplasia and atypical proliferation of mucosa.

Adult↗

PubMed2000

[Clinical study of Astragalus injection plus ligustrazine in protecting myocardial ischemia reperfusion injury].

OBJECTIVE: To investigate the mechanism of Astragalus injection plus ligustrazine in preventing the occurrence of myocardial ischemia reperfusion injury (MIRI) during open heart surgery of cardiopulmonary bypass, and the treating principle of MIRI in TCM. METHODS: Twenty-four patients suffering from either valvular heart diseases or congenital ventricular septal defect were randomly divided into three treated groups (Astragalus, ligustrazine, Astragalus plus ligustrazine) and the control group, 6 in each group. Blood samples were taken via subclavian central vein at the time before anesthesia (T1), 10 minutes of occlusion of aorta (T2), 10 minutes (T3) and 30 minutes (T4) after the release, and the end of operation (about 180 minutes after release, T5) respectively; EKG was observed, and the levels of asparate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), MB isoenzyme of CK (CK-MB), malondialdehyde (MDA) and the activity of superoxide dismutase (SOD), nitric oxide (NO), nitric oxide synthetase (NOS) were determined. RESULTS: The treated groups could reduce the levels of AST, LDH, CK, CK-MB, MDA, SOD compared with the control group, particularly Astragalus plus ligustrazine, there had significant difference (P < 0.05, P < 0.01). In NO activity improvement, Astragalus plus ligustrazine won the best effect, Astragalus group the next. CONCLUSIONS: The mechanism of MIRI is Qi deficiency and blood stasis in TCM, its treating principles should be promoting Qi and removing the blood stasis. According to the authors' study, Astragalus plus ligustrazine could effectively protect against MIRI, which is better than using the 2 medicines separately.

Adolescent↗

PubMed2000

[Therapeutic effect of weikangfu on gastric precancerous disorder with spleen deficiency syndrome and its effect of gastric mucosal zinc, copper, cyclic adenosine monophosphate, superoxide dismutase, lipid peroxide and 3H-TdR lymphocyte conversion test].

OBJECTIVE: To observe the clinical therapeutic effect of Weikangfu granule (WKF) on intestinal metaplasia (IM), atypical proliferation (ATP) and its therapeutic mechanism. METHODS: Sixty-one patients (treated group) were diagnosed by integrated TCM and western medicine (WM), and treated according to Syndrome Differentiation, the amount of WKF was adjusted according to various Syndromes. The control group consisted of 54 patients, took Weisu granule without considering their Syndromes. Besides, 15 healthy subjects comprised of the control group. Gastroscopy was conducted both before and after the treatment, mucosal sample was taken from antral region of stomach for pathological changes, for IM classification through histochemical staining and for determination of gastric mucosal Zn, Cu, cAMP, SOD, serum LPO and 3H-TdR lymphocyte conversion test (LCT). RESULTS: (1) The symptomatic and pathologic therapeutic effect of the treated group were markedly better than those of the control group (P < 0.05, P < 0.01). (2) The levels of gastric mucosal Zn, Cu, cAMP, SOD and 3H-TdR LCT of the treated group before treatment were all lower than that of the healthy control group, but they were markedly increased after treatment, while serum LPO, however, increased before treatment, significant difference existed between the above groups (P < 0.05, P < 0.01). CONCLUSION: WKF can reverse IM and ATP of Spleen Deficiency Syndrome through the mechanism of improving the level of gastric mucosal Zn, Cu, cAMP and SOD, promoting cytodifferentiation, improving cytoimmunity, reducing superoxide and LPO.

Adult↗