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Biomedical subjects

W Zegarski

Publications and source records attributed to W Zegarski.

At least 19 recordsLinked to original sources
PubMed1995

[Interpretation of diagnostic data exemplified by tests for identification of Helicobacter pylori in gastric mucosa].

Seven diagnostic tests were employed to detect the infection of gastric mucosa with the H. pylori bacteria, among patients with epigastric pain and dyspeptic complains. The tests' results were compared to the bacteriologic culture. For each test the following criteria were taken into consideration: sensitivity, specificity, positive and negative predictive values. The tests' accuracy was compared with the agreement expected by chance and beyond chance (kappa). For each test the likelihood ratio was calculated for its positive and negative results. The construction of so called diagnostic decision tree was presented, which allows on the evaluation of the diagnostic test and its utility. The best result were obtained from the test, in which the identification of bacteria took place in the histopathologic specimen after its Gram staining. The interpretation of clinical significance of diagnostic data is simplified with the use of likelihood ratio calculated for a positive test result.

Adult↗
PubMed1994

[Use of 4 microbiological methods to detect Helicobacter pylori].

The presence of Helicobacter pylori (HPY) was confirmed in 90% of a group of 76 patients with gastric disorders. The methods included the direct urease test, a preparation directly stained by the Gram method, culture and serological study using Cobas Core Anti-H. pylori EIA. High levels of IgG HPY antibodies were found in the serum of patients with gastritis chronica and ulcus duodeni. Urease activity can be observed even after 48 hours. Nevertheless such interpretation of test results requires confirmation by other microbiological methods applied simultaneously. HPY was obtained in 50% of culture. Already one hour after the test started, it was possible to confirm this species on the basis of Rapidec pylori. The presented procedure can be used and performed in a laboratory cooperating effectively with a gastroenterologist.

Adult↗
PubMed1992

DNA base modifications in chromatin of human cancerous tissues.

Free radical-induced damage to DNA in vivo is implicated to play a role in carcinogenesis. Evidence exists that DNA damage by endogenous free radicals occurs in vivo, and there is a steady-state level of free radical-modified bases in cellular DNA. We have investigated endogenous levels of typical free radical-induced DNA base modifications in chromatin of various human cancerous tissues and their cancer-free surrounding tissues. Five different types of surgically removed tissues were used, namely colon, stomach, ovary, brain and lung tissues. In chromatin samples isolated from these tissues, five pyrimidine-derived and six purine-derived modified DNA bases were identified and quantitated by gas chromatography/mass spectrometry with selected-ion monitoring. These were 5-hydroxy-5-methylhydantoin, 5-hydroxyhydantoin, 5-(hydroxymethyl)uracil, 5-hydroxycytosine, 5,6-dihydroxycytosine, 4,6-diamino-5-formamidopyrimidine, 8-hydroxyadenine, xanthine, 2-hydroxyadenine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine, and 8-hydroxyguanine. These compounds are known to be formed typically by hydroxyl radical attack on DNA bases. In all cases, elevated amounts over control levels of modified DNA bases were found in cancerous tissues. The amounts of modified bases depended on the tissue type. Lung tissues removed from smokers had the highest increases of modified bases above the control levels, and the highest overall amounts. Colon cancer tissue samples had the lowest increases of modified bases over the control levels. The results clearly indicate higher steady-state levels of modified DNA bases in cancerous tissues than in their cancer-free surrounding tissues. Some of these lesions are known to be promutagenic, although others have not been investigated for their mutagenicity. Identified DNA lesions may play a causative role in carcinogenesis.

Chromatin↗
PubMed1990

[Psychosocial conditions of psychotropic drug poisoning of patients treated in the Gdańsk Toxicology Center].

The purpose of the study was in analysis of both causes and social conditioning leading to attempts of self-intoxications by the use of psychotropic drugs. The study included 234 patients treated in the Gdańsk Toxicological Center during years 1982-1987. In a majority, the intoxications referred to young subjects up to 30 year of age, more frequently women than men. Only 10% of subjects studied have had university education. The alcohol or drug dependence and various psychiatric disturbances has been noted in a majority of cases studied. The lack of real suicidal determination was characteristic pattern of group observed.

Adolescent↗
PubMed1983

Acute silver poisoning through inhalation.

The paper presented a case of acute occupational poisoning with silver vapours. The course of the ailment was very serious. The clinical picture corresponded to the syndrome of the "shock lung"--with extreme respiratory insufficiency. The treatment included administration of steroids, heparin, antibiotics and controlled respiration with a positive endexpiratory pressure (PEEP). The patient was completely cured and fully regained physical fitness. Our observation seems to shake the hitherto predominant view of non-toxicity of metallic silver or its vapours.

Adult↗
PubMed1970

[Pancreatitis].

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Acute Disease↗