Search PubMed⌕ Search

Biomedical subjects

W Ye

Publications and source records attributed to W Ye.

125 records · Page 7Linked to original sources
PubMed1995

[Protection from procarbazine induced testicular toxicity by gonadal steroid hormones].

To confirm the claims that the procarbazines (PCZ) effect on stem spermatogonia can be overcome by gonadal steroid hormones,mature,LBNF1 male rats were implanted with silastic capsules containing testosterone (T 200 mm) + estradiol (E 50 mm). The silastic capsules of control and TE groups were removed 24 hours after the PCZ injection and the rats were sacrificed 9 weeks later. The functional status of the testis and the hormonal parameters at the time of the injection of PCZ were measured and correlated with the degree of protection ,as measured by the recovery of sperm counts. The results indicated that testosterone and estradiol does protect spermatogenesis from PCZ intoxification. To determine whether the protection is specific for stem spermatogonia, we also measured body weight loss 3-4 days, lymphocyte counts 1 day, and spermatocyte count 9 days after PCZ treatment. There were no differences in the dose response curves of these three assays between the hormone and sham treated rats. The lack of protection indicates that the hormonal protection of stem spermatogonia was not due to the alterations in either systemic or testicular pharmacokinetics of spermatocyte. We conclude that A3 spermatogonia and preleptotene spermatocyte are sensitive to PCZ and there is no evidence of hormonal protection for the differentiated spermatogonia and preleptotene spermatocyte.

Animals↗
PubMed1994

Antispermatogenic effect of Tripterygium wilfordii and tripchlorolide (T4) on rat gametogenesis and spermatozoa.

The multiglycosides of Tripterygium wilfordii (TII), a ready-made Chinese herbal medicine used for the treatment of rheumatoid arthritis, have been shown to cause oligospermia in patients. In the present study, the antifertility effects of TII and tripchlorolide (T4, isolated from TII) were observed in male rats. In rats fed with TII at a dose of 10 mg.kg.d for 7 weeks, the seminiferous tubules were essentially not influenced. However, most of the sperm heads along the surface of the tubular lumen were transformed from the normal sickle-shaped to round shaped, suggesting a possible mutagenic action. There was minimal testicular change but prominent epididymal spermatozoa damage in all rats treated with T4 (0.05 mg.kg.d) for 7 weeks. The epididymal spermatozoa showed various structural abnormalities, including disrupted connecting pieces and cracked midpieces, and more than 80% of the spermatozoa were decapitated. No significant changes were seen in the main visceral organs. The data suggest that T4 may have good prospects as a male contraceptive.

Animals↗
PubMed1993

Effect of intra-vas deferens injection of gossypol-polylactic acid on fertility and spermatogenesis in rats.

In order to reduce gossypol's side effects, we applied a controlled drug release system. In this system gossypol is carried on a polymer of polylactic acid which controls drug release and can also be injected into the vas deferens so that the drug can act continuously and directly on spermatogenesis. We used a single dose of gossypol-polylactic acid (0.35 mg/0.1 ml for each vas deferens) which is only 0.2% of the usual antifertility dose. Fertility tests showed that male rats became infertile 4 weeks after intravasal injection of gossypol-polylactic acid. Gross and microscopic findings in the heart, liver, kidneys and lungs revealed no evidence of pathologic change. Treated rats gained body weight as fast as did the controls. The majority of spermatozoa showed breakage of the axial fibers, indicating that gossypol had diffused into the testes. The fertility of the treated rats recovered four to eight months after treatment. Gossypol-polylactic acid injected into the vas deferens exhibited a low degree of toxic side effects and a high antifertility activity, and is therefore an important approach to decreasing the side effects while enhancing the antifertility specificity of gossypol.

Animals↗
PubMed1992

[Pathogenetic and clinical significance of peripheral activated T-lymphocyte subsets in Basedow's disease].

The peripheral activated T-cell subsets which express concurrently DR and CD antigens were determined in 22 patients with Graves' disease using the fluorescence-rosette double labelling technique. In the active phase of the disease a decrease in the percentage of CD8+ cells, an increase in the CD4+/CD8+ ratio and an elevation of various DR+CD+T-lymphocytes was found. A concurrent marked increase in DR+CD4+ cells accounts for an abnormality in the equilibrium of activated T-lymphocyte subsets and an increase in DR+CD4+/DR+CD8+ ratios. In comparison with the active phase the DR+CD3+ and DR+CD4+ cells decreased in remission, but DR+CD8+ cells remained unchanged. The decrease in the ratio of CD4+/CD8+ cells was less marked than that of the DR+CD4+/DR+CD8+ cells, which fell into the normal range. Our results suggest an excessive activation of T-helper cells as well as a deficiency in T-suppressor cells which may play an important role in the pathogenesis of Graves' disease.

Adult↗
PubMed1992

The actions of capsaicin applied topically to the skin of the rat on C-fibre afferents, antidromic vasodilatation and substance P levels.

1. Single applications of solutions of capsaicin were made to the intact skin of anaesthetized rats and the effects on cutaneous blood flow and the firing of C-nociceptor afferents determined. Blood flow was measured by laser-Doppler flowmetry. C-fibre activity was recorded from filaments dissected from the saphenous nerve. 2. Following the application of a capsaicin solution (concentration > or = 1 mM) to rat saphenous skin, low frequency firing occurred in C-polymodal nociceptors that sometimes continued for > 10 min. At the some time, large increases in skin blood flow occurred exceeding 300% in some instances. 3. After the initial excitation, some C-polymodal nociceptors lost their sensitivity to pressure whilst their sensitivity to heat was lost or enhanced depending on the vehicle used. 4. Sensitivity of C-polymodal nociceptors to heat recovered in < 1 day following a single application of 33 mM capsaicin. Thresholds to mechanical pressure, however, were still significantly elevated by 123% on day 1, but had recovered on day 2. 5. Vasodilatation in response to saphenous nerve stimulation ('antidromic vasodilatation') was significantly reduced by 35%, 2 days after a single application of 33 mM capsaicin, but was normal at 4 days. 6. Following a single application of 33 mM capsaicin, skin substance P levels fell to only half the normal value at day 1 and remained at this level throughout the 4 day period examined. 7. It is suggested that the ability of relatively low concentrations of capsaicin to desensitize C-fibre nociceptors may underlie the analgesic action of topical capsaicin in man.

Administration, Topical↗
PubMed1991

[Antispermatogenic effects of multiglycosides of Tripterygium wilfordii and monomer T4 in the testes and epididymal spermatozoa of rats].

The antispermatogenic effects of the multiglycosides of Tripterygium wilfordii (GTW) and monomer T4 isolated from GTW on the testes and epididymal spermatozoa of rats were examined microscopically following silver staining. The dosages of GTW and T4 were 10mg/kg.d and 0.05 mg/kg.d, respectively, fed for 7 weeks via gastric intubation. Different degrees of damage to the testis and epididymal tubes were found in rats treated with GTW. Deformed, round-headed sperms and exfoliated cells (mainly spermatids and occasionally pachytene spermatocytes) were seen in the tubular lumen of the epididymis. Spermatids with deformed acrosomal vesicles were also commonly observed. No significant pathological changes were seen in other internal organs. T4 mainly caused damage to the spermatozoa in the epididymis. Under the dosage of 0.05mg/kg.d the sperm count was almost nil, but no discernible changes in the seminiferous tubules, epididymal epithelia, Leydig cells or Sertoli cells were found. The antifertility potency of T4 was 200 times stronger than that of GTW.

Animals↗
PubMed1991

[A study on components and compound prescription of huangqin decoction].

The results obtained from the pharmacological experiments have preliminarily demonstrated that the compound prescription is better than its individual component drug in the range and potency of pharmacological activities. In the prescription Radix Scutellariae plays a principal role in pharmacological effects. Radix Scutellariae and Radix Paeoniae Alba can potentiate each other. Radix Glycyrrhizae and Fructus Jujubae in the compound prescription also exhibit biological activities to some extent. The relieving effect of the prescription for spasm and pain may result from the synergistic interaction between Radix Paeoniae Alba and Radix Glycyrrhiza, and the heat clearing and diarrhea stopping action is due to the biological activity of Radix Scutellariae.

Animals↗
PubMed1990

[Treatment and prevention of asymmetrical intrauterine growth retardation with huoxue huayu prescription].

The purpose of this study was to observe the therapeutical and preventive effect of the compound prescriptions of Huoxue Huayu (promoting blood circulation to remove stasis, PBCRS) and Yiqi Huoxue, replenishing Qi to remove stasis, RQRS) on experimental intrauterine growth retardation (IUGR) caused by passive smoking during pregnancy in rats. The fetal average birth weight and the erythrocyte deformability were found to be significantly reduced in the smoke-exposed group compared with the control group. However, there was a significant increase of blood viscosity (at both shear rates), Hb, Ht, MCV, MCH levels and the erythrocyte fragility in the smoke-exposed group in comparison with the control group. After the mother-rats were given PBCRS and RQRS prescriptions during gestation, the fetal average birth weight and erythrocyte deformability were markedly increased. The blood viscosity, Hb, Ht, MCV, MCH levels and erythrocyte fragility of pregnant rats were decreased. No differences were found in the control group and the two treated groups. The electronic microscope observation of erythrocyte shape shows there were some protrusions on the surface of red cells in the smoke-exposed group. The protrusions were significantly reduced in the two treated groups, which was similar to the control group. The erythrocyte deformability, the blood viscosity, Hb, Ht, MCV, MCH levels indicate that there was a significant correlation between them and the fetal average birth weight respectively. This study indicates that blood stasis is one of the pathogenetic mechanisms of asymmetrical IUGR and that the PBCRS recipe could improve intrauterine growth environment of fetal which could treat and prevent IUGR in obstetrics.

Animals↗
PubMed1990

[A preliminary study on the pharmacology of the compound prescription huangqin tang and its component drugs].

Results obtained from the pharmacological experiments demonstrated that the compound prescription Huangqin Tang and its component drugs, roots of Paeonia lactiflora, Scutellaria baicalensis and Glycyrrhiza uralensis, and the fruit of Ziziphus jujuba showed marked anti-inflammatory effect. The compound prescription and its component drugs, except the peony root, possessed significant antispastic effect. Antipyretic effect of Radix Scutellariae, Radix Glycyrrhizae and Huangqin Tang was suggested. Huangqin Tang, Radix Paeoniae alba, and Radix Glycyrrhizae showed marked analgesic effect. In addition, the experiments confirmed that both Huangqin Tang and single Radix Scutellariae have sedative effect.

Animals↗
PubMed1984

Waterborne outbreak of rotavirus diarrhoea in adults in China caused by a novel rotavirus.

During two epidemics of acute diarrhoea in China in late 1982/early 1983, more than 12 000 adults in two coal mining districts were affected. The virus isolated from stool samples resembled a rotavirus morphologically. Antigenically it lacked the group antigen shared by known rotaviruses. Like other rotaviruses it had a double-stranded RNA with 11 discrete segments, but the pattern of migration of the segments on polyacrylamide electrophoresis differed from those of other rotaviruses.

Adolescent↗
PubMedDate not supplied

Multipoint linkage of 9 anonymous probes to HPRT, factor 9, and fragile X.

We have analyzed the segregation of restriction fragment length polymorphisms (RFLPs) associated with 9 anonymous probes detecting loci DXS10, DXS15, DXS19, DXS37, DXS51, DXS52, DXS98, DXS99, and DXS100 and probes for HPRT and F9 in a set of 40 families segregating fragile X (fra(X]. Using two-point and multipoint analysis, we have established their relative genetic locations. The results indicate that DXS99 and DXS10, unlike previous reports, are not tightly linked to F9. A new locus was found to map within the F9 - fra(X) region. DXS98 showed 6% recombination with fra(X) and appeared to be the closest locus to fra(X). These results will be useful for mapping the relative position of newly defined X probes in this region and for future genetic studies of families with fra(X), hemophilia B, or Lesch-Nyhan mutations.

Chromosome Mapping↗
PubMedDate not supplied

Repeat hepatic cryotherapy for metastatic colorectal cancer.

This study evaluated the risks and benefits of repeat hepatic cryotherapy for recurrent, unresectable hepatic metastases from colorectal carcinoma. Review of a prospective database identified 195 patients who underwent hepatic cryotherapy for metastatic colorectal carcinoma during a 7-year period. Of the 14 patients who underwent successful repeat cryotherapy for recurrences confined to the liver, 86% had Duke's stage D colorectal carcinoma at initial diagnosis. The median age of the 14 patients was 58 years (range 41 to 77 years). The median number of hepatic metastases was three at the first cryotherapy and two at the second cryotherapy. At a median follow-up of 71 months, the mean survival times from original diagnosis, first cryotherapy, and second cryotherapy were 53, 42, and 19 months, respectively. At the most recent follow-up, eight patients (57%) have died of their disease, four (29%) are alive with disease, and two (14%) have no evidence of disease. The mean interval between the first and second cryotherapies was 23 months. The complication rates after the first and second cryotherapies were 7% and 14%, respectively. One patient developed a wound dehiscence after the first cryotherapy. Following the second cryotherapy, one patient had a small bowel obstruction and another had a pleural effusion. There was no perioperative mortality. Repeat cryotherapy for recurrent, unresectable hepatic metastases from colorectal cancer is safe and improves survival. However, a prospective trial is needed to validate the efficacy of systemic therapy and to better define the indications for repeat hepatic cryotherapy.

Adult↗