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Biomedical subjects

W Yang

Publications and source records attributed to W Yang.

At least 559 records · Page 31Linked to original sources
PubMed1990

Neodymium: YAG laser therapy in aphakic pupillary block glaucoma and aphakic malignant (ciliovitreal block) glaucoma.

Aphakic pupillary block glaucoma and malignant glaucoma (ciliovitreal block) are severe complications of the intracapsular cataract extraction, presenting clinically as elevated intraocular pressure, persistent shallow anterior chamber and severe vitreal hernia. Six eyes (four women and two men) with aphakic pupillary block glaucoma and aphakic malignant glaucoma were resolved by Neodymium: YAG laser iridotomy and hyaloidotomy. The laser therapy resulted in immediate deepening of the anterior chamber and reducing of intraocular pressure. No side effect was observed. Neodymium: YAG laser iridotomy and hyaloidotomy may be more effective and simple than penetrating surgery in such cases.

Aged↗
PubMed1990

[Analgesic and anti-inflammatory effects and toxicity of houjianxi].

It is effective for acute and chronic laryngopharyngitis clinically. Houjianxi can raise the pain thresholds during hot-plate test in mice, tail flick test in rats, and writhing test in mice with single dose ig 0.125 to 2.0 g/kg. Houjianxi can also inhibit the swelling of hind paw induced by sc 1% carrageenin 0.1 ml in rats as well as the increased vascular permeability induced by ip 0.7% HAC in mice. Toxic side effects were not observed for Houjianxi ig 48 g/kg x 3 within a day in mice and 1.8 g/kg daily for 90 d in rats.

Animals↗
PubMed1989

Crystallization and characterization of human chorionic gonadotropin in chemically deglycosylated and enzymatically desialylated states.

Crystals suitable for X-ray diffraction studies at moderate resolution have been grown from two forms of human chorionic gonadotropin (hCG): HF-treated hCG and neuraminidase-treated hCG. The enzymatically desialylated form of hCG produced crystals that diffract to 2.8 A as compared to the HF-treated hCG crystals that diffract to 3.0 A. Although it was assumed that the high and heterogeneous carbohydrate content of the glycoprotein hormones inhibited their crystallization, this report suggests that it is the negatively charged surface sugars and neither the total carbohydrate content nor its heterogeneity which interferes with crystal formation. Chemical deglycosylation resulted in significantly increased protein degradation during crystal growth. Such peptide bond cleavages were observed to a much lesser extent in the crystals grown from neuraminidase-digested hCG. Sequence analysis of the HF-treated hCG crystals suggested that up to 45% of the molecules within the crystal had an acid-labile peptide bond cleaved. In contrast, the neuraminidase-treated hCG exhibited less than 9% of this type of cleavage. The increase in heterogeneity of the polypeptide chains within both crystals over that existent in the starting proteins was apparently due to changes occurring during crystal growth. The manner in which hCG was treated prior to crystallization was found to be a very important factor in the extent of peptide bond cleavages occurring during crystal growth. HF treatment of glycoproteins may render glycoproteins more susceptible to peptide bond cleavages during crystal growth.

Amino Acid Sequence↗
PubMed1989

Aspirin intolerance.

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Anti-Inflammatory Agents, Non-Steroidal↗
PubMed1988

Identification of 5-hydroxytryptamine1A receptor proteins in bovine frontal cortex.

5-Hydroxytryptamine1A (5-HT1A) receptor proteins were identified by a novel approach in which photoaffinity labeling technique was used in conjunction with affinity column chromatography. 5-HT1A receptors were solubilized from bovine frontal cortical membranes with 0.3% digitonin and 0.1% Nonidet P-40, and bound effectively to 1-[2-(4-aminophenyl)ethyl]-4-(3-trifluoromethylphenyl)piperazine (PAPP)-coupled Affi-Gel 10 in a time-dependent manner. PAPP was shown previously to be a selective ligand for the 5-HT1A receptor. Two protein bands with molecular masses of approximately 55,000 and 38,000 daltons revealed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis were eluted from the affinity column with either 1 mM 5-HT or 1 microM [3H]1-[2-(4-azidophenyl)ethyl]-4-(3-trifluoromethyl-phenyl)piperazine ([3H]p-azido-PAPP). [3H]p-Azido-PAPP is a selective photoaffinity labeling probe for the 5-HT1A receptor. The intensity of these two protein bands and the incorporation of [3H]p-azido-PAPP into these two proteins decreased significantly when the solubilized fraction was preincubated with excess 5-HT or PAPP (saturating all 5-HT1A receptors) prior to affinity column chromatography. These results suggest strongly that these two proteins are related to the 5-HT1A receptor protein. The isoelectric points of the photolabeled 5-HT1A receptor proteins were 6.0 and 6.5.

Affinity Labels↗
PubMed1988

Reliability and validity of a Chinese computerized diagnostic instrument.

This paper reports on the development and testing of a new Chinese diagnostic instrument--the Adult Diagnostic Interview Schedule-Present (ADIS-P). This instrument uses a selection of items adapted from DIS and SADS. It is administered within approximately 25 min by a trained clinician using a small portable computer that provides an immediate diagnosis. The instrument was tested on 1,331 patients from six large mental hospitals across the country. The concurrent validity was good: compared to the concensus diagnosis of two senior clinicians (who were blinded to the ADIS-P result), the overall diagnostic accuracy was 83% and the weighted Kappa value was 0.60. The 1 month test-retest reliability was satisfactory (weighted Kappa = 0.55). We conclude that ADIS-P is a convenient, reliable and valid diagnostic instrument.

Adult↗