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Biomedical subjects

W Y Lui

Publications and source records attributed to W Y Lui.

At least 199 records · Page 11Linked to original sources
PubMed1988

Effects of gastric cancer cells on lymphocyte proliferation.

Lack of lymphocyte infiltration into gastric cancer tissue appears to be an ominous prognostic indicator. The effects of gastric cancer cells on PHA-induced lymphocyte proliferation were studied. Peripheral lymphocytes were co-cultured for 72 hours with either gastric cancer cells or normal mucosal cells. Pairs of cancerous and normal mucosal cells from stomachs of eight patients, were separately co-cultured with peripheral lymphocytes either from patients or from normal volunteers. The degree of PHA-induced lymphocyte proliferation was measured by 3H-thymidine incorporation. The lymphocyte proliferation was inhibited by the presence of either gastric cancerous or normal mucosal cells in a dose-related manner. The lymphocytes from the normals proliferated twice as much as did the lymphocytes from the patients. The isotope incorporation occurred in lymphocytes rather than in gastric cells since the later incorporated insignificant amounts of isotope. There was no difference between gastric cancerous or normal mucosal cells inhibiting the proliferation of either normal or patients' lymphocytes (p greater than 0.05). In conclusion, gastric cancerous cells (up to 10(6)/ml) have no enhanced inhibition on lymphocyte proliferation when compared with normal gastric mucosal cells.

Aged↗
PubMed1988

Alterations of humoral immunity in patients with gastric cancer.

For better understanding of the alterations of humoral immunity in gastric cancer patients, IgG, IgA, IgM, complement C3, C4, CH50, natural antibody (isohemagglutinin-IgM class), ESR, CRP, albumin and globulin were quantitated in sera taken preoperatively from 81 patients with gastric cancer and from 29 control patients with hernia. The results from patients with gastric cancer were grouped according to pTNM staging (including stage I + II, III, and IV). Serum globulin and IgG levels in all stages of cancer patients were significantly lower than that of the controls (p less than 0.05). The CRP and ESR levels in stage III and IV cancer patients were significantly higher (p less than 0.05). There was no difference between cancer and hernia patient groups in IgA, IgM, isohemagglutinin-IgM class, C3, C4, CH50, albumin, WBC and total lymphocyte counts. In conclusion, the significant changes in humoral immunity in patients with gastric cancer include: (1) decrease in serum IgG and globulin levels, and (2) increased levels of acute phase reactants (ESR, CRP). These results imply that patients with gastric cancer have lower acquired humoral immunity and have acute phase reactions.

Adult↗
PubMed1987

The effects of arginase on neoplasm. I. The role of arginase in the immunosuppressive effects of extract from gastric cancer.

Arginase, a potent immune inhibitor, is studied in gastric cancer for its possible role in immunosuppression. Aqueous extracts (n = 12) from gastric cancer tissue inhibited PHA-induced lymphocyte proliferation more than those from normal gastric mucosal tissues (p less than 0.05). The inhibition was not due to cytotoxic effect of the extracts. Gastric arginase was isolated from gastric extracts by an affinity column conjugated with an anti-arginase antibody. The arginase from both normal and cancer gastric tissues strongly inhibited lymphocyte proliferation. By using enzyme-immunoassay, the arginase contents in cancer tissues were determined and showed 3 times as much as that in normal gastric mucosal tissues (n = 14) (p less than 0.005). The increased arginase content in cancer tissue was further confirmed by immunohistochemistry study. Arginase exists abundantly amounts in the cytoplasm of cancer cells, much more than that of normal cells (n = 30). It is conceivable that the depressed cellular immunity in gastric cancer patients might be partly due to the abundant amount of arginase in cancer cells.

Arginase↗
PubMed1986

The clinical aspect of retained gastric antrum.

Thirty symptomatic patients with retained gastric antrum proved pathologically and/or by isotopic visualization were studied and treated from 1968 to 1983. The latent periods from the antral exclusion to the occurrence of anastomotic ulcers after a subtotal gastrectomy with Billroth's type II reconstruction varied from a few days to 19 years, with an average of 2.8 years. Fasting serum gastrin levels were normal in 14 of 21 patients and were intermittently high in some patients. The basal to maximal acid-output ratio was greater than 0.6 in 69% of the patients; primary cimetidine treatment was effective in three of five patients. Thirty-five operations on 27 patients were divided into six groups; all of these 27 patients eventually underwent resection of retained gastric antrum. We concluded that resection remains the best treatment for anastomotic ulcer related to retained gastric antrum. Additional truncal vagotomy did not provide additional benefit to these patients. Furthermore, cimetidine can be useful to control the symptoms for preoperative preparation or definitive treatment in high-risk patients.

Adult↗
PubMed1986

Carcinoid tumor of the pancreas causing the diarrheogenic syndrome: report of a case combined with multiple endocrine neoplasia, type I.

A case of carcinoid tumor of the pancreas with the watery diarrhea, hypokalemia, and hypochlorhydria syndrome in association with hyperparathyroidism and the amenorrhea-galactorrhea syndrome is presented. Resection of the three grossly enlarged, hyperplastic parathyroid glands restored eucalcemia in this patient. A subsequent excision of the 370 gm pancreatic carcinoid tumor resulted in a cure of the watery diarrhea and a return of the gastric acid secretion to normal. Immunocytochemical studies of the pancreatic tumor demonstrated a positive stain only for serotonin, and negative results for vasoactive intestinal polypeptide, pancreatic polypeptide, glucagon, insulin, cholecystokinin, gastrin, and calcitonin were obtained. These studies suggest that in this patient, serotonin was a causative agent of the watery diarrhea syndrome.

Adenoma, Islet Cell↗
PubMed1985

Expression of c-myc gene in human hepatoma.

The level of c-myc transcript was examined in liver samples from seven hepatoma patients. Transcripts were detected in all the normal liver parts examined; in contrast, in two hepatoma parts, there was a dramatic reduction in c-myc transcripts. The restriction enzyme pattern of c-myc gene appeared the same among samples. The data suggest that c-myc gene expression might not be required for the maintenance of the tumor state in human liver carcinogenesis.

Carcinoma, Hepatocellular↗
PubMed1985

Haemophilic pseudotumours of the mandible: report of a case in a one-year-old child.

Pseudotumours of bone are an uncommon complication of severe haemophilia and only seven cases occurring in the mandible have been reported previously in the English literature. If treated early the condition appears to have a better prognosis and so it should be included in the differential diagnosis of any slowly progressive swelling of hard and soft tissues occurring in a patient with severe haemophilia. However aspiration and biopsy should be avoided if possible. Previous authors have advocated a variety of invasive procedures for this condition. A further case is described which is unique in the age at which the bilateral lesions developed, in the successful outcome of conservative management and in the dental observations.

Diagnosis, Differential↗
PubMed1984

High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector.

A chemically synthesized gene for human interferon-gamma has been cloned into a prokaryotic expression vector under the regulation of a synthetic constitutive transcriptional-translational control unit that contains a strong bacteriophage T5 early promoter and a strong ribosome-binding site. Cells harboring the recombinant plasmid express high levels (4 X 10(9) units per liter of culture) of antiviral activity specific for interferon-gamma. Analysis of total cell lysates on NaDodSO4/polyacrylamide gels revealed a 17,200-dalton protein, expected for the nonglycosylated form of human interferon-gamma, that constitutes greater than 15% of total cell protein.

Amino Acid Sequence↗