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Biomedical subjects

W Y Kim

Publications and source records attributed to W Y Kim.

88 records · Page 5Linked to original sources
PubMed1994

Three-dimensional visualization of velocity profiles in the human main pulmonary artery with magnetic resonance phase-velocity mapping.

Detailed data on blood velocity fields in the normal human main pulmonary artery are an essential platform for discriminating physiologic from pathologic pulmonary flow patterns. Over the years, many studies have revealed quite inconsistent data mainly because of lack of suitable measuring techniques. By using combined cardiac- and respiratory-triggered magnetic resonance phase velocity mapping, very consistent data were obtained in 12 volunteers. In all subjects the location of the highest axial velocities was shifted from the inferior-right toward the superior-left part of the vessel area during the right ventricular contraction, with rapidly decreasing velocities to the inferior right evolving into retrograde flow in the deceleration phase. The mean temporal velocity profile was consistently skewed with a low flow region also toward the inferior-right vessel wall. The magnetic resonance phase shift method used in this study provided remarkably consistent high-quality data about human pulmonary artery velocity fields. This is most likely because of the use of combined cardiac and respiratory triggering.

Adult↗
PubMed1994

Two-dimensional mitral flow velocity profiles in pig models using epicardial Doppler echocardiography.

OBJECTIVES: This study investigated the velocity distribution across the natural mitral valve. BACKGROUND: Information about the blood velocity distribution across the mitral valve is of interest in basic fluid dynamic studies of the natural mitral valve and is needed for precise cardiac output estimates by Doppler echocardiography. METHODS: The velocity distribution across the mitral valve was measured by epicardial Doppler echocardiography in ten 90-kg anesthetized pigs. By rotating the ultrasound transducer in 30 degrees intervals from the apical position, we constructed two-dimensional velocity profiles across the left ventricular inflow tract from diameters from each rotation arranged around a reference point. The time-averaged mitral velocity profile was calculated to estimate the error in cardiac output calculations that may occur with pulsed Doppler ultrasound when a single sample volume is used to record the mean velocity across the mitral orifice. RESULTS: The time-averaged diastolic cross-sectional mitral velocity profiles at the level of the mitral annulus and leaflet tips were variably skewed because of the development of a large anterior vortex in the left ventricle during the deceleration of early diastolic inflow and atrial systole. The ratio of the time-velocity integral of the center sample volume to the spatially averaged time-velocity integral was 1.13 +/- 0.15 (mean +/- SD) (range 0.80 to 1.32). Using regression analysis, we found a correlation between the degree of nonuniformity of the cross-sectional velocity distribution and the peak velocity of the anterior vortex (r = 0.65, p < 0.01). CONCLUSIONS: The assumption of a flat mean velocity profile across the mitral valve can introduce errors of +13 +/- 15% (mean +/- SD) in cardiac output measured with pulsed Doppler ultrasound when one is interrogating a single center sample volume.

Animals↗
PubMed1994

Studies by pulsed Doppler ultrasonography of velocity fields downstream of graded stenoses on the abdominal aorta in pigs.

PURPOSE: To investigate local hemodynamics downstream of arterial stenoses, a perivascular five-element Doppler ultrasound transducer was used for registration of one-dimensional velocity profiles and estimation of Reynolds (turbulent) normal stresses downstream of smooth, graded stenoses on the abdominal aorta in six 90 kg pigs. METHODS: Blood velocities were registered by a 10 MHz pulsed Doppler velocimeter that used a modified zero-crossing detector with an upper -3 dB cutoff frequency of 200 Hz. Signal analysis included ensemble averaging, turbulence analysis, and dimensional visualization of velocity profiles. RESULTS: Velocity profiles downstream of minor (< or = 40%) and moderate (40% to 65%) stenoses were skewed with the highest systolic velocities toward the anterior vessel wall and diastolic flow reversal occasionally present at the posterior vessel wall. Immediately downstream of severe (> or = 65%) stenoses a prominent poststenotic jet and systolic recirculation zones were present. Further downstream, vortices and eddies dominated the flow field. Reynolds normal stresses were highest at locations in the velocity field with high-velocity gradients corresponding to the parajet zone. CONCLUSIONS: The present study demonstrated that pulsed Doppler ultrasonography can provide detailed and quantitative information of flow phenomena such as jetlike flow, vortices, and recirculation zones in a poststenotic flow field in the abdominal aorta.

Animals↗
PubMed1994

What force can the myocardium generate on a prosthetic mitral valve ring? An animal experimental study.

Data on the magnitude of forces which can be generated by the myocardium on a prosthetic heart valve ring are not available from the literature. Therefore, we implanted strain gauge mounted 29 mm original specification Edwards-Duromedics mitral valve prostheses in 13 pigs. The valves were implanted in both anatomic and anti-anatomic orientation. In order to estimate the forces on the valve ring, acute in vivo measurements of the dynamic deformation of the valve ring were performed and correlated with the stiffness of the same valves measured in vitro. In the post cardioplegic heart of the anesthetized pigs there was a maximum force developed by the myocardium of 6-8 N on the valve ring with a resulting maximum deformation of 40 microns with valves mounted in the anatomic position. These findings have implications for design of future mechanical mitral valves and for mitral rings used for mitral valvuloplasty. These data can also be used as reference for evaluation of safety limits in existing valves in terms of their physical properties. Based on the direction of the maximum myocardial force acting on the mitral valve ring and the difference in compliance of the valve ring along the pivotal or orthogonal axis, it is indicated from that these acute porcine studies that bileaflet valves in the mitral position are subjected to less deformation when implanted 60 degrees counter-clockwise to the native mitral intercommisural line.

Animals↗
PubMed1993

Three dimensional visualisation of velocity profiles in the normal porcine pulmonary trunk.

OBJECTIVE: The aim was to obtain detailed data of velocity profile development in the porcine pulmonary trunk. METHODS: Hot film anemometry was used for point blood velocity measurements in the entire cross sectional area one diameter downstream of the normal porcine pulmonary valve. Computerised three dimensional visualisations of the spatial and temporal development of blood velocity profiles were made. Measurement series were conducted under spontaneous and stressed haemodynamic conditions in 16 pigs with a body weight of approximately 90 kg. RESULTS: The velocity profiles revealed consistent temporal and spatial development characteristics. In the systolic acceleration phase the profile was flat, but during peak systole an initially counterclockwise rotation was seen; subsequently the profile turned clockwise and ultimately counterclockwise to become flat during early and late systolic deceleration phases. CONCLUSIONS: These skewed, rotating velocity profiles are in conflict with the generally accepted assumption that the velocity profile in the pulmonary trunk is flat. The rotating skewness of the velocity profile in the porcine pulmonary trunk has not been described before. The reason for the rotating velocity profile is obscure.

Animals↗
PubMed1993

Molecular typing of hepatitis C virus genome from sera and liver tissues of patients with anti-HCV positive chronic liver disease.

The authors investigated the distribution of HCV genotypes in patients with various chronic liver diseases in Korea. Study population was 70 individuals, positive for second generation anti-HCV EIA, consisting of 37 cases with sporadic non-A, non-B (NANB) chronic hepatitis (CH), 12 NANB hepatocellular carcinoma, 16 post-transfusion NANB hepatitis, 4 non-B blood donors and 1 healthy family member of a patient with sporadic CH. Molecular typing was performed by RT-nested PCR with type-specific primer sets deduced from the NS-5 region of HCV. The prevalence of type II was 75.0% and type III was 25.0% in sera. In liver tissues, type II HCV was shown in 63.0%, type III HCV in 3.7% and co-infections with type II and III HCV were observed in 18.5% of 27 samples biopsied. In the sera of patients with chronic hepatitis, typing results were relatively well correlated with those in tissues (75%), but type III could not be observed. Among 12 HCC patients, type III HCV appeared only in tissues, not in sera. These results suggest that type II HCV may be the major HCV type in Korea, and co-infections with type II and-III HCV may not be rare in chronic liver diseases with HCV.

Base Sequence↗
PubMed1989

The major late promoter of adenovirus-2 is accurately transcribed by RNA polymerases IIO, IIA, and IIB.

Subunit IIa of mammalian RNA polymerase II contains at its C terminus 52 tandem repeats of the consensus sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser. This domain is unmodified in RNA polymerase IIA, extensively phosphorylated in RNA polymerase IIO, and absent from RNA polymerase IIB. In an effort to define the role of the C-terminal domain, we have measured the transcriptional activity of purified RNA polymerases IIO, IIA, and IIB. The ability of each polymerase subspecies to transcribe the major late promoter of adenovirus-2 was examined in a polymerase-dependent transcription system reconstituted from partially purified transcription factors. RNA polymerases IIO, IIA, and IIB are all capable of initiating specific transcripts from this promoter. The transcriptional activity was determined as a function of the concentration of RNA polymerase II, template DNA, and each of the essential general transcription factors. The transcriptional activities of RNA polymerases IIA and IIB were comparable and consistently greater than that of RNA polymerase IIO when assayed under the conditions described here. The kinetics of transcript formation is similar except that RNA polymerase IIO has a more pronounced lag. These results show that the C-terminal domain of subunit IIa is not essential for the accurate initiation of transcripts from the major late promoter of adenovirus-2 and that the effect of the C-terminal domain is not likely mediated by the general transcription factors required for the expression of class II genes.

Adenoviridae↗
PubMed1988

Purification of RNA polymerase IIO from calf thymus.

Three subspecies of RNA polymerase II, designated IIO, IIA, and IIB, have been described in calf thymus and shown to differ in the apparent molecular weight of their largest subunits, designated IIo, IIa, and IIb, respectively. The objective of this study was to develop a procedure for the purification of RNA polymerase IIO. This form of the enzyme predominates in vivo and is responsible for the transcription of most cellular genes. RNA polymerase II is solubilized from isolated calf thymus nuclei in the presence of high concentrations of chelators, precipitated with polyethyleneimine, extracted with salt, and precipitated with (NH4)2SO4. The solubilized enzyme is resolved from factors that destabilize RNA polymerase IIO by chromatography on heparin-Sepharose CL-4B and DE52. RNA polymerase IIO is then partially resolved from RNA polymerases IIA and IIB by chromatography on DEAE-5PW and further purified by chromatography on Phenyl-Superose and Mono Q. RNA polymerase IIO was purified 1000-fold from the polyethyleneimine eluate resulting in about 130 micrograms of RNA polymerase IIO from 300 g of calf thymus. The specific activity of RNA polymerase IIO, in nonselective assays using calf thymus DNA as template, is 440 units/mg and not significantly different from that of RNA polymerases IIA and IIB. The similar transcriptional activities in nonselective assays suggest that the C-terminal domain of the largest RNA polymerase II subunit does not play a major role in the elongation phase of the reaction when deproteinized DNA serves as template. The small subunits of RNA polymerase IIO are indistinguishable from those of RNA polymerases IIA and IIB.

Animals↗
PubMed1986

Immunochemical analysis of mammalian RNA polymerase II subspecies. Stability and relative in vivo concentration.

Three subspecies of RNA polymerase II, designated IIO, IIA, and IIB, have been described in a variety of eukaryotic cells and shown to differ in the molecular weight of their largest subunit, designated IIo, IIa, and IIb, respectively. The objectives of this study were to establish the in vivo molecular structure of RNA polymerase II in mammalian cells and to examine conditions that influence the stability of RNA polymerase II subspecies. Subunit affinity-purified antibodies were used to determine the relative concentration of subunits IIo, IIa, and IIb in crude extracts of calf thymus tissue, cultured bovine kidney cells, and HeLa cells. HeLa cells contain exclusively RNA polymerase IIO whereas both cultured bovine kidney cells and calf thymus tissue contain RNA polymerases IIO and IIA. RNA polymerase IIB was not detected at significant levels in any of the cell extracts examined. Cell extracts were aged at either 4 degrees or 37 degrees C and the stability of RNA polymerases IIO and IIA determined by protein blotting. In the presence of buffer normally used for RNA polymerase purification, subunit IIo disappears from calf thymus extracts within 24 h at 4 degrees C or within 5 min at 37 degrees C. RNA polymerase IIO is partially stabilized by the inclusion of protease inhibitors and further stabilized by the presence of relatively high concentration of EDTA and EGTA. The prior fractionation of nuclei does not have an appreciable effect on RNA polymerase II stability. An increase in the amount of reducing agent causes a dramatic reduction in the stability of subunit IIo. The following manuscript (Bartholomew, B., Dahmus, M. E., and Meares, C. F. (1986) J. Biol. Chem. 14226-14231) examines the transcriptional activity of RNA polymerases IIO and IIA in reactions dependent on the major late promoter of adenovirus-2. Photoaffinity labeling of subunits IIo and IIa, relative to their concentration in the transcription extract, indicates that the transcriptional activity of RNA polymerase IIO is greater than 10 times that of IIA.

Animals↗
PubMedDate not supplied

Teratoid Wilms tumor in childhood: CT and ultrasonographic appearances.

Teratoid Wilms tumor is defined as an unusual variant of nephroblastoma, in which there is a significant diversity of cell types and tissues in a neoplasm, where areas of classic nephroblastoma tissue are also present. We report a case of teratoid Wilms tumor demonstrated as a unilateral cystic and solid renal mass containing fatty tissue on ultrasonography and computed tomography.

Child, Preschool↗
PubMedDate not supplied

Numerical simulation and experimental validation of blood flow in arteries with structured-tree outflow conditions.

Blood flow in the large systemic arteries is modeled using one-dimensional equations derived from the axisymmetric Navier-Stokes equations for flow in compliant and tapering vessels. The arterial tree is truncated after the first few generations of large arteries with the remaining small arteries and arterioles providing outflow boundary conditions for the large arteries. By modeling the small arteries and arterioles as a structured tree, a semi-analytical approach based on a linearized version of the governing equations can be used to derive an expression for the root impedance of the structured tree in the frequency domain. In the time domain, this provides the proper outflow boundary condition. The structured tree is a binary asymmetric tree in which the radii of the daughter vessels are scaled linearly with the radius of the parent vessel. Blood flow and pressure in the large vessels are computed as functions of time and axial distance within each of the arteries. Comparison between the simulations and magnetic resonance measurements in the ascending aorta and nine peripheral locations in one individual shows excellent agreement between the two.

Adult↗
PubMedDate not supplied

Effect of administration of ketorolac and local anaesthetic infiltration for pain relief after laparoscopic-assisted vaginal hysterectomy.

The efficacy of local anaesthetic infiltration and/or non-steroidal anti-inflammatory drugs for post-operative analgesia following laparoscopic-assisted vaginal hysterectomy (LAVH) was investigated in 83 patients, randomized into four groups in this double-blind, placebo-controlled study: group BK, local infiltration with bupivacaine and pre-incisional intramuscular (IM) ketorolac; group NN, saline local infiltration IM; group BN, local infiltration with bupivacaine and saline IM; group NK, local infiltration with saline and ketorolac IM. Post-operative pain scores were assessed at 1 h, 3 h, 6 h, 12 h and 24 h using a visual analogue scale (VAS). The major pain site, first analgesic request time and incidence of analgesic requests were also recorded. At 1 h, 3 h and 6 h after surgery, group BK patients had significantly lower VAS pain scores than group NN patients. The first analgesic request time was significantly longer in group BK than in groups NN, BN and NK. Pre-incisional treatment with ketorolac IM and local infiltration with bupivacaine reduced post-operative pain after LAVH.

Adult↗
PubMedDate not supplied

Magnetic resonance coronary lumen and vessel wall imaging.

Coronary magnetic resonance angiography (MRA) is a technique aimed at establishing a noninvasive test for the assessment of significant coronary stenoses. There are certain boundary conditions that have hampered the clinical success of coronary MRA and coronary vessel wall imaging. Recent advances in hardware and software allow for consistent visualization of the proximal and mid portions of the native coronary arteries. Current research focuses on the use of intravascular MR contrast agents and black blood coronary angiography. One common goal is to create a noninvasive test which might allow for screening for major proximal and mid coronary artery disease. These novel approaches will represent a major step forward in diagnostic cardiology.

Contrast Media↗