[Automatic hemacytometer applied for detecting white blood cell in the patients with severe jaundice hepatitis].
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Cholestasis, Intrahepatic↗
Biomedical subjects
W Xie
Publications and source records attributed to W Xie.
[Malignant melanoma of external ear: 14 cases report].
OBJECTIVE: To improve the diagnosis and treatment level of malignant melanoma in external ear. METHOD: 14 cases with malignant melanoma of external ear were reported. All patients were treated by surgical excision. RESULT: 13(in 14) cases were followed up. 7 patients were survival with no tumor, the others died. The 5-year survival rate for the whole series was 50.0%. CONCLUSION: The malignant melanoma of external ear has rapid progress and poor prognosis, early detection and treatment are necessary. Extensive excision of malignant melanoma was principle method of the treatment. Recurrent tumor should be treated by combined operation with radiation.
Adult↗
[Immunohistochemical study of safe resection margin in the surgical treatment of laryngeal carcinoma].
OBJECTIVE: To study safe resection margin of laryngectomy according to the level of protein of gene expression. METHOD: Using citric-LSAB-immunochemical technique, the expressions of ras, c-myc and P53 were studied in different regions of larynx including 30 cases laryngeal carcinoma, border area, adjacent mucosa which was 0.5, 1.0, 1.5, 2.0 cm away from cancer and four normal laryngeal mucosa. RESULT: The results showed that both single expression and co-expression of p21, p62 and p53 increased as tissue progressed in sequence from normal mucosa, 2.0, 1.5, 1.0, 0.5 cm distant mucosa adjacent to carcinoma, border area and carcinoma. A significant different expression in most of them can be seen at regions of 0.5 cm and 1.0 cm distant from the tumor (P < 0.05). CONCLUSION: The tissue 0.5 cm adjacent to the tumor should be regarded as precancerous lesion. It is appropriate to regard 0.5 cm away from tumors as resection margins for surgical treatment of laryngeal carcinoma.
Adult↗
[Neurilemmoma of the nasal cavity and paranasal sinuses (report of 12 cases)].
OBJECTIVE: To further understand the neurilemmoma of nose. METHOD: 12 cases of neurilemmomas of the nasal cavity and paranasal sinuses were treated successfully by operation, 2 cases of malignant change lesions were treated by extensive resection and radiation. RESULT: 11 in 12 cases were followed up. 9 cases were cured and no any serious complications, 2 cases of malignant tumor were died. CONCLUSION: Early stage diagnosis is important. CT scan should be made as essential examination, complete surgical resection is the choice of treatment for primary lesions; malignant change lesions should be treated by combined operation with radiation.
Adolescent↗
[Studies on the infrared spectra of rare earth complexes with pyruvic acid].
FT-IR spectra of La, Ce, Pr, Nd, Sm, Eu, Gd, Dy, Ho and Y complex with pyruvic acid have been studied. The principal infrared absorption peaks are assigned for the region 400- 2 000 cm(-1). Three IR peaks in the region 200-400 cm(-1) are shifted obviously to higher frequency with increasing of the atomic number of rare earth ion, these were believed to be RE-O bond stretching mode absorptions. The IR spectra also indicated that the carboxylate groups have the form of chelation bridging tridentate to rare earth ions, i. e., one of the carboxyl oxygens was shared between two adjacent metal ions, moreover, the splitting and broad bands are existed in IR spectra of the rare earth complexes. The RE-O bond of the complexes is principally ionic.
English Abstract↗
[Molecular cloning and characterization of novel protein kinase gene DYRK3].
OBJECTIVE: To isolate full length cDNA of a novel protein kinase and to deduce the protein kinase's classification position and functions. METHODS: cDNA libraries gDNA library was screened with a partial cDNA clone which is homologous to human protein kinase DYRK2 as probe. FISH mapping was performed. RESULTS: Two full length cDNAs of a novel protein kinase from human muscle cDNA library and human testis cDNA library were isolated. The full length cDNA from muscle has an open reading frame which is predicted to encode a protein of 588 amino acid residues and the cDNA from testis to encode a protein of 568 amino acid residues. CONCLUSION: Because the sequence from the 27th codon to the 3' end of the cDNA from muscle is identical to that from the 7th codon to the 3' end of the cDNA from testis, they should be different transcripts of the same gene. As the gene is highly homologous to human protein kinase DYRK2, the present authors termed the gene DYRK3. DYRK3 is homologous to many serine/threonine protein kinases such as yeast Yak1, human Clk1, human Mnb, drosophila melanogaster Mnb and Cdk2. DYRK3 should belong to the Clk family in CMGC group of serine/threonine protein kinase. DYRK3 has been mapped to chromosome 1q32 by FISH.
Amino Acid Sequence↗
Guanine nucleotide exchange factor GEF115 specifically mediates activation of Rho and serum response factor by the G protein alpha subunit Galpha13.
Signal transduction pathways that mediate activation of serum response factor (SRF) by heterotrimeric G protein alpha subunits were characterized in transfection systems. Galphaq, Galpha12, and Galpha13, but not Galphai, activate SRF through RhoA. When Galphaq, alpha12, or alpha13 were coexpressed with a Rho-specific guanine nucleotide exchange factor GEF115, Galpha13, but not Galphaq or Galpha12, showed synergistic activation of SRF with GEF115. The synergy between Galpha13 and GEF115 depends on the N-terminal part of GEF115, and there was no synergistic effect between Galpha13 and another Rho-specific exchange factor Lbc. In addition, the Dbl-homology (DH)-domain-deletion mutant of GEF115 inhibited Galpha13- and Galpha12-induced, but not GEF115 itself- or Galphaq-induced, SRF activation. The DH-domain-deletion mutant also suppressed thrombin- and lysophosphatidic acid-induced SRF activation in NIH 3T3 cells, probably by inhibition of Galpha12/13. The N-terminal part of GEF115 contains a sequence motif that is homologous to the regulator of G protein signaling (RGS) domain of RGS12. RGS12 can inhibit both Galpha12 and Galpha13. Thus, the inhibition of Galpha12/13 by the DH-deletion mutant may be due to the RGS activity of the mutant. The synergism between Galpha13 and GEF115 indicates that GEF115 mediates Galpha13-induced activation of Rho and SRF.
3T3 Cells↗
Specific involvement of G proteins in regulation of serum response factor-mediated gene transcription by different receptors.
Regulation of serum response factor (SRF)-mediated gene transcription by G protein subunits and G protein-coupled receptors was investigated in transfected NIH3T3 cells and in a cell line that was derived from mice lacking Galphaq and Galpha11. We found that the constitutively active forms of the alpha subunits of the Gq and G12 class of G proteins, including Galphaq, Galpha11, Galpha14, Galpha16, Galpha12, and Galpha13, can activate SRF in NIH3T3 cells. We also found that the type 1 muscarinic receptor (m1R) and alpha1-adrenergic receptor (AR)-mediated SRF activation is exclusively dependent on Galphaq/11, while the receptors for thrombin, lysophosphatidic acid (LPA), thromboxane A2, and endothelin can activate SRF in the absence of Galphaq/11. Moreover, RGS12 but not RGS2, RGS4, or Axin was able to inhibit Galpha12 and Galpha13-mediated SRF activation. And RGS12, but not other RGS proteins, blocked thrombin- and LPA-mediated SRF activation in the Galphaq/11-deficient cells. Therefore, the thrombin, LPA, thromboxane A2, and endothelin receptors may be able to couple to Galpha12/13. On the contrary, receptors including beta2- and alpha2-ARs, m2R, the dopamine receptors type 1 and 2, angiotensin receptors types 1 and 2, and interleukin-8 receptor could not activate SRF in the presence or absence of Galphaq/11, suggesting that these receptors cannot couple to endogenous G proteins of the G12 or Gq classes.
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5↗
Tec/Bmx non-receptor tyrosine kinases are involved in regulation of Rho and serum response factor by Galpha12/13.
A transient transfection system was used to identify regulators and effectors for Tec and Bmx, members of the Tec non-receptor tyrosine kinase family. We found that Tec and Bmx activate serum response factor (SRF), in synergy with constitutively active alpha subunits of the G12 family of GTP-binding proteins, in transiently transfected NIH 3T3 cells. The SRF activation is sensitive to C3, suggesting the involvement of Rho. The kinase and Tec homology (TH) domains of the kinases are required for SRF activation. In addition, kinase-deficient mutants of Bmx are able to inhibit Galpha13- and Galpha12-induced SRF activation, and to suppress thrombin-induced SRF activation in cells lacking Galphaq/11, where thrombin's effect is mediated by G12/13 proteins. Moreover, expression of Galpha12 and Galpha13 stimulates autophosphorylation and transphosphorylation activities of Tec. Thus, the evidence indicates that Tec kinases are involved in Galpha12/13-induced, Rho-mediated activation of SRF. Furthermore, Src, which was previously shown to activate kinase activities of Tec kinases, activates SRF predominantly in Rho-independent pathways in 3T3 cells, as shown by the fact that C3 did not block Src-mediated SRF activation. However, the Rho-dependent pathway becomes significant when Tec is overexpressed.
3T3 Cells↗
Regulation of Ca2+-dependent Cl- conductance in a human colonic epithelial cell line (T84): cross-talk between Ins(3,4,5,6)P4 and protein phosphatases.
1. We have studied the regulation of whole-cell chloride current in T84 colonic epithelial cells by inositol 3,4,5,6-tetrakisphosphate (Ins(3,4,5,6)P4). New information was obtained using (a) microcystin and okadaic acid to inhibit serine/threonine protein phosphatases, and (b) a novel functional tetrakisphosphate analogue, 1, 2-bisdeoxy-1,2-bisfluoro-Ins(3,4,5,6)P4 (i.e. F2-Ins(3,4,5,6)P4). 2. Calmodulin-dependent protein kinase II (CaMKII) increased chloride current 20-fold. This current (ICl,CaMK) continued for 7 +/- 1.2 min before its deactivation, or running down, by approximately 60 %. This run-down was prevented by okadaic acid, whereupon ICl,CaMK remained near its maximum value for >= 14.3 +/- 0.6 min. 3. F2-Ins(3, 4,5,6)P4 inhibited ICl,CaMK (IC50 = 100 microM) stereo-specifically, since its enantiomer, F2-Ins(1,4,5,6)P4 had no effect at >= 500 microM. Dose-response data (Hill coefficient = 1.3) showed that F2-Ins(3,4,5,6)P4 imitated only the non-co-operative phase of inhibition by Ins(3,4,5,6)P4, and not the co-operative phase. 4. Ins(3,4,5,6)P4 was prevented from blocking ICl,CaMK by okadaic acid (IC50 = 1.5 nM) and microcystin (IC50 = 0.15 nM); these data lead to the novel conclusion that, in situ, protein phosphatase activity is essential for Ins(3,4,5,6)P4 to function. The IC50 values indicate that more than one species of phosphatase was required. One of these may be PP1, since F2-Ins(3,4,5,6)P4-dependent current blocking was inhibited by okadaic acid and microcystin with IC50 values of 70 nM and 0.15 nM, respectively.
Calcium↗
Cell cycle-dependent subcellular localization of the TSG101 protein and mitotic and nuclear abnormalities associated with TSG101 deficiency.
TSG101 is a recently discovered tumor susceptibility gene whose functional inactivation in mouse fibroblasts results in cell transformation and the ability to form metastatic tumors in nude mice. Although restoration of TSG101 activity reverses tumorigenesis, neoplasia is irreversible in some cells, suggesting that permanent genetic alteration can occur during TSG101 inactivation. Here we describe studies that support this notion. We find that localization of TSG101 is cell cycle-dependent, occurring in the nucleus and Golgi complex during interphase, and in mitotic spindles and centrosomes during mitosis; cells made neoplastic by a deficiency in TSG101 expression show a series of mitosis-related abnormalities, including multiple microtubule organizing centers, aberrant mitotic spindles, abnormal distribution of metaphase chromatin, aneuploidy, and nuclear anomalies. Our findings suggest that TSG101 deficiency may lead to genome instability in addition to previously reported reversible neoplastic transformation.
Aneuploidy↗
Cloning and characterization of the gene encoding beta subunit of mitochondrial processing peptidase from the basidiomycete Lentinula edodes.
We have isolated the gene encoding the beta subunit of mitochondrial processing peptidase (beta-MPP) from the shiitake mushroom Lentinula edodes. It is a nuclear gene with two small introns. Comparison with known beta-MPP genes revealed that the L. edodes gene is most closely related with that from Neurospora crassa, with 60.8% identities and 87% similarity in the amino-acid sequences. The deduced L. edodes beta-MPP peptide sequence contains the inverse zinc-binding motif (H-X-E-H) that has been found in a large family of zinc-binding metalloproteinases including bacterial proteinases, insulin degrading enzymes and beta-MPPs. The two histidines are thought to contribute two of the three residues for zinc binding. The expression of L. edodes beta-MPP is higher during the development of the fruiting bodies, suggesting that higher mitochondrial activities may be required to meet the energy demand in the rapid growth of the fruiting bodies.
Amino Acid Sequence↗
Expression of the integrin-linked kinase (ILK) in mouse skin: loss of expression in suprabasal layers of the epidermis and up-regulation by erbB-2.
Integrin-linked kinase (ILK) is a newly identified serine/threonine protein kinase implicated in integrin signaling. To investigate the functions of ILK in vivo, we have analyzed the expression and regulation of ILK in the skin, in which proper control of cell-extracellular matrix interactions and cell proliferation is essential for its normal development and homeostasis. We report here that ILK is abundantly expressed throughout the extracellular matrix-rich dermis. ILK mRNA was also detected in the hair follicles and the basal cells of the interfollicular epidermis. However, ILK expression is lost in the suprabasal layers of keratinocytes that are undergoing terminal differentiation. PINCH, an ILK-binding protein, exhibited a similar expression pattern in the skin. Recent studies have indicated that erbB-2, a member of the epidermal growth factor receptor family, plays a pivotal role in epidermal growth, differentiation, and hair follicle morphogenesis. Using a transgenic mouse system in which an activated erbB-2 is overexpressed in the epidermis, we show that ILK expression is regulated by erbB-2. The in vivo expression and regulation patterns of ILK, together with its biochemical activities, suggest an important role of ILK in coordinating the integrin signaling pathways and the growth factor signaling pathways in the development of the skin and the pathogenesis of skin diseases.
Adaptor Proteins, Signal Transducing↗
Discrimination of spatial displacements by patients with retinitis pigmentosa.
We compared maximum displacement thresholds (Dmax) with minimum displacement thresholds (Dmin) in patients with retinitis pigmentosa (RP) in order to characterize the nature of their visual disability, as well as to assess possible models of foveal vision loss. Thresholds for discriminating the direction of the spatial displacement of random dot patterns were measured in a group of 20 patients with typical RP or Usher syndrome whose visual acuities were 20/40 or better and who had minimal or no clinical evidence of changes in the ocular media. Findings were compared with those from an age-similar group of 15 visually normal subjects. Displacement thresholds were measured using a two-frame random dot cinematogram and a four-alternative forced-choice procedure. Measurements were made at each of three dot contrasts and three dot sizes. For the patients with RP, reducing either the dot contrast or dot size increased Dmin and decreased Dmax such that the range of discriminable displacements became considerably restricted, even at modest reductions in dot contrast or size. This restriction in the displacement thresholds of the patients with RP was correlated significantly with their visual acuity. By comparison, the control subjects showed little change in either Dmin or Dmax under these conditions. These results indicate that patients with RP who have only relatively minor reductions in their visual acuity can have severely compromised motion perception. The pattern of findings suggests that an abnormal contrast response of the foveal cone system is a major determinant of the impaired displacement thresholds of these patients with RP.
Adult↗
Primary structure and expression of a chicken laminin beta chain: evidence for four beta chains in birds.
Characterization of a full length cDNA sequence for a chicken laminin beta chain is described which is most closely related to the mammalian beta 2 chain. Comparison with published sequences shows that the chicken beta 2-like chain corresponds to a fragment of a previously described laminin beta chain called B1-2 (O'Rear, 1992). The sequence of the chicken beta 2-like chain differed from fragments of two other chicken laminin beta chains that were previously described and designated B1-1 (now called beta 1; O'Rear, 1992) and beta x (Ybot-Gonzalez et a1.,1995). In addition, the beta 2- like chain does not appear to be the chicken equivalent of the mammalian laminin beta 3 chain, since it differs markedly in cDNA sequence, possesses domain IV and has a transcript size of 6 kb. We therefore propose that there are at least four laminin beta chains in the chicken. Sequence comparison of the beta 2-like laminin chain with previously cloned beta 1 and beta 2 chains shows a somewhat closer relationship to rat and human beta 2 than to mouse and human beta 1, especially in domains I, II and alpha. In addition, two expressed fragments of the chicken beta 2-like chain were recognized by a monoclonal antibody (C4) regarded as specific for the rat beta 2 chain (Hunter et al., 1989a). The results therefore suggest that the laminin chain previously described as a potentially novel chain called B1-2 (O'Rear, 1992) is likely to be the chicken equivalent of the mammalian beta 2 chain.
Amino Acid Sequence↗
Mutations in the gene encoding gap junction protein beta-3 associated with autosomal dominant hearing impairment.
Hearing impairment is the most commonly occurring condition that affects the ability of humans to communicate. More than 50% of the cases of profound early-onset deafness are caused by genetic factors. Over 40 loci for non-syndromic deafness have been genetically mapped, and mutations in several genes have been shown to cause hearing loss. Mutations in the gene encoding connexin 26 (GJB2) cause both autosomal recessive and dominant forms of hearing impairment. To study the possible involvement of other members of the connexin family in hereditary hearing impairment, we cloned the gene (GJB3) encoding human gap junction protein beta-3 using homologous EST searching and nested PCR. GJB3 was mapped to human chromosome 1p33-p35. Mutation analysis revealed that a missense mutation and a nonsense mutation of GJB3 were associated with high-frequency hearing loss in two families. Moreover, expression of Gjb3 was identified in rat inner ear tissue by RT-PCR. These findings suggest that mutations in GJB3 may be responsible for bilateral high-frequency hearing impairment.
Adult↗
Effect of noise contrast polarity and temporal asynchrony on visual sensitivity.
We evaluated the effect of substitutive noise on contrast sensitivity within the context of linear (Fourier) and nonlinear (non-Fourier) visual processes. Orientation judgments for D6 (sixth spatial derivative of Gaussian) patterns were obtained from three visually normal subjects when random regions of the target and background were occluded by small (1.7 arc min) pixel arrays that were either all of the same contrast polarity or a mixture of equal percentages of negative and positive contrast. The target was presented either synchronously or asynchronously with the occluding elements. Our results indicate that the manipulation of noise characteristics in this way can bias performance either toward a nonlinear process that is insensitive to noise contrast polarity but sensitive to temporal asynchrony or toward a quasi-linear process that is sensitive to noise contrast polarity but insensitive to temporal asynchrony. These findings have relevance to models of the effect of spatial sampling on the visual performance of persons with retinal disease.
Adult↗
[Relation between abnormal histogram and automatic blood platelet count].
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Adolescent↗