Diabetes mellitus treated by massage.
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Acupuncture Points↗
Biomedical subjects
W Wu
Publications and source records attributed to W Wu.
[Study on certified reference material of benzene, toluene, o-xylene in charcoal].
This certified reference material (CRM) of benzene, toluene and o-xylene in charcoal is used for quility control and calibration of analytical instruments in the determination of benzene, toluene and o-xylene in air. Homogeneity of benzene, toluene and o-xylene concentrations in the CRM was testes by GC. The stability of the CRM was assessed for one year. The certified values are based on analysis made by a group of independent laboratoies using GC method.
Benzene↗
[Immunoprotection in guinea pigs using DNA recombinant plasmid rpDJt and expressed protein P68 in L. interrogans serovar lai].
Immunoprotection against the infection by Leptospira interrogans serogroup Icterohemorrhagiae serovar lai strain 017 was demonstrated in guinea pigs vaccinated with DNA recombinant plasmid rpDJt and expressed protein P68 derived from genomic library of Leptospira strain 017. Thirty days after active immunization, each group received intraperitoneally (1/2 dose) and subcutaneously (1/2 dose) inoculum of L. interrogans serovar lai stain 017; cultures were adjusted to 5 x 10(8) cells/ml. All guinea pigs were observed for 10 days after challenge. Survival (%) of P68 group was 100(7/7); P23 group was 75(3/4); group rpDJt was 77(10/13); group lack recombinant (control) pT7-7 was 25 (3/12), and group with whole-cell inactivated vaccine was 93(13/14). Although the protective antigen in the leptospires has yet to be determined, it is evident that expressed protein P68 conferred a high degree of immunoprotection in guinea pigs.
Animals↗
[Grouping of thoracic lymph nodes on CT scan according to American Thoracic Society classification].
OBJECTIVE: To assess the usefulness of the classification developed by the American Thracic Society (ATS) in the grouping of thoracic lymph nodes on CT scan. METHODS: Ten patients suffered from lymphoma (5 cases), sarcoidosis (2 cases) and metastatic lymphadenopathy (3 cases) were examinated with CT. The intrathoracic lymph nodes were grouped according to the ATS nodal classification. RESULTS: Six areas were divided on the CT scans: (1) between the upper margin of the sixth cervical vertebra and the pulmonary apex, including nodes in group 1R/L; (2) between the pulmonary apex and the upper margin of the aortic arch, including nodes in group 2R/L and 6; (3) between the upper margin of aortic arch and the carina, including nodes in group 4R/L, 5, 6 and 10R; (4) within 3 cm below carina, including nodes in group 7 and 10R/L; (5) between 3 cm from subcarina and the top of diaphragm, including nodes in group 8R/L and 14R/L; (6) intrapulmonary area, distal to the upper lobe bronchi, including nodes in group 11R/L. CONCLUSION: The division of lymph nodes into six areas on CT scan makes the ATS lymph node classification more easily applicable.
Humans↗
[Changes of GABA immunoreactivity in aged rat cochlear nucleus].
OBJECTIVES: To detect the changes of gama-aminobutyric acid (GABA) immunoreactivity in the aged rat cochlear nucleus and to investigate its relationship with the initiation of presbycusis. METHODS: The cochlear nucleuses of adult (2-3 months) and aged (20-36 months) Wistar rats were stained with streptavidin/peroxidase (S-P) technique. GABA-positive neurons were counted with a computer-aid image analysis system. RESULTS: Most of the GABA-immunoreactive cell bodies were found in the superficial layers of the dorsal cochlear nucleus (DCN). The number of GABA-positive neurons decreased significantly in the DCN of the aged rat cochlear nucleus (P < 0.001). CONCLUSION: The reduction of GABA positive neurons in the aged rat cochlear nucleus may be related to presbycusis.
Aging↗
[Protein tyrosine kinase (PTK) activities during the induction of apoptosis by arsenic trioxide (As2O3)].
OBJECTIVE: To explore the mechanism of arsenic trioxide (As2O3) induced apoptosis in K562 cell line. METHODS: Immunoprecipitation was used to obtain BCR/ABL and ABL proteins. The activities of PTK and tyrosine phosphorylation were measured by biochemical method and Western blot, respectively. RESULTS: As2O3 reduced the PTK activities of cytosol and membrane proteins and BCR/ABL and ABL proteins. The tyrosine phosphorylation of 180,000 and 125,000 proteins declined. CONCLUSION: As2O3 may interfere the signal transduction of BCR/ABL protein by reducing its PTK activity and induce K562 cell apoptosis.
Apoptosis↗
[Diagnosis and treatment of Ramsay Hunt syndrome (a report of 39 cases)].
Thirty-nine cases with Ramsay Hunt syndrome were presented, in which 23 cases were firmly diagnosed early, and others were misdiagnosed to be Bell's palsy in 9 cases, sudden sensorineural hearing loss in 5, herpes zoster pharyngitis in 1 and acute suppurative otitis media in 1, respectively. All patients were treated with prednisone or dexamethasone for 3 weeks. The results of treatment were as follows: complete recovery in 27 cases, residual facial paralysis in 12 patients, in which 11 had sensorineural hearing loss. We concluded that: 1. When patients present idiopathic facial paralysis associated with objective sensorineural hearing loss, Hunt syndrome should be suspected even in the absence of vesicles. 2. Treatment with steroid and antiviral agent is needed. There is no significant difference in the effects between oral and intravenous steroid therapy. 3. The poor prognosis may relate with severe facial paralysis accompanying severe hearing loss. 4. Acoustic stapedius reflex in patients with mild or no hearing loss is useful for defining the involved sites and evaluating the prognosis.
Adolescent↗
[Significance of medical imaging findings in sphenoid sinus disease to pituitarism].
To explore the relationship between medical imaging finding in sphenoid sinus disease and pituitarism, medical images in sphenoid sinus of 7 patients with pituitarism were analysed. Three cases had mass, and the other 4 cases had inflammatory disease in sphenoid sinus. However, their pituitary images were all normal. Sphenoid sinus disease is significant to pituitarism, but may be overlooked. Radiologists must pay more attention to imaging finding in sphenoid sinus.
Adolescent↗
[Quantitative determination of extracellular glutathione and cysteine in gerbil brain by microdialysis and high performance liquid chromatography with electrochemical detection].
A method for the determination of glutathione and cysteine in microdialysate by HPLC with electrochemical detector has been described. The column used was Hypersil ODS, 5 microns, 200 mm x 4.6 mm i.d. and mobile phase was 50% 0.2 mol/L KCl, 10.6% 0.2 mol/L HCl, 39.4% methanol and 0.1 mmol/L EDTA (pH 2.0) with a flow rate of 1 mL/min. The microdialysis probe (membrane: pc, length: 10 mm, diameter: 0.5 mm/CMA) was inserted into the right striatum of gerbil at the following coordinates: 1.5 mm anterior and 1.5 mm lateral to bregma, and 3.5 mm below dura. Electrochemical pretreatment of a glassy carbon electrode used as a working electrode in an electrochemical detector has been found to enhance the analytical capability of detector for the determination of glutathione and cysteine. The quantitative determination was made with an electrochemical detector at 0.9 V and by external standard method. The method gave good recoveries and reproducibilities. The average recoveries of two analytes were 91.4% and 87.3% respectively. Extracellular fluid glutathione and cysteine concentrations in the striatum of gerbil brain were determined by the microdialysis and chromatography described in this study. The contents of Cys and GSH in microdialysate of gerbil striatum were 65.54 +/- 12.96 nmol/L and 68.59 +/- 13.23 nmol/L. The results were comparable with those reported in previous works.
Animals↗
[Study of test method of A, B and H substances in human tissues].
With the help of the red cell adherence test, absorption-inhibition test and absorption-elution test, the A, B, O blood type of different tissues of corpses, whose ABO blood-type was known, was examined. The Substance of A, B, H type in 16 kinds of tissues of corpses was the same as in blood stain of corpses. The checked time limit of A, B, H substance of tissues preserved in different temperature was studied. The checked time of A, B, H substance of the tissues in 4 degrees C was longer than in room temperature, and the time in the hollow organ (vissera) shorter than that in parenchymatous organ (vissera).
ABO Blood-Group System↗
[Follow-up study on influence of Sini Decoction to living quality of patients after percutaneous transluminal coronary angioplasty].
OBJECTIVE: To determine the influence of Sini Decoction (SND) on living quality of patients after percutaneous transluminal coronary angioplasty (PTCA). METHODS: Randomized case-control clinical trail was conducted to evaluate the living quality of 40 post-PTCA patients before and after SND treatment by scoring. RESULTS: The scores in physical symptoms, sense of well-being, depression, satisfaction of life and work capacity of patients after PTCA were improved significantly as compared with before PTCA, P < 0.05-0.01. Scores of patients received SND treatment were higher than those untreated with SND in the first three criteria, P < 0.05, particularly on relieving palpitation and dyspnea. CONCLUSION: SND is helpful to improve the living quality of patients after PTCA.
Angioplasty, Balloon, Coronary↗
[Reliability design and analysis of man-machine performance of display and illumination system in cockpit].
This paper analysed and discussed the basic reliability of display and illumination system in which Chinese characters displayed on LCD were read and the colours were identified, and instruments in different visual views and the instruments of differential display models were read. The colour rendering of the light source was also evaluated. Basic reliability may be chosen as 0.9995.
Aircraft↗
[The relationship of facet orientation to intervertebral disc protrusion and lateral recess stenosis in lower lumbar spine].
OBJECTIVE: To investigate the relationship of facet orientation to intervertebral disc protrusion and lateral recess stenosis in lower lumbar spine. METHOD: The relationship between facet geometry (joint angle and tropism) and disc protrusion, lateral recess stenosis was investigated with computer tomography (CT) at the vertebral levels L(3 - 4), L(4 - 5), and L(5) - S(1). 772 facet joint angles (386 lower lumbar levels of 136 patients) were measured on coronal CT scans by transverse inter-facet angle (TIFA). RESULT: There was no statistically significant relationship between facet joint asymmetry and disc protrusion (P > 0.05). Disc protrusion occurred more frequently on side of sagittally oriented facet joint than coronal side (P < 0.01). The patients whose lumbar transverse inter-facet angle less than 20 degrees easily suffered from degenerative lumbar lateral recess stenosis in the elderly. The TIFA in Asian less evident than in European may be the important cause for high incidence of lumbar lateral recess syndrome in Asia. CONCLUSION: Stress on the lower lumbar spine leads to disc protrusion. No association is found between facet joint asymmetry and lumbar disc protrusion. The asymmetry of facet joint will influence the direction of intervertebral disc protrusion at level from L(4) - S(1). The patient whose lower lumbar spine inter-facet angle may unlikely suffer from degenerative lumbar lateral recess stenosis.
Adolescent↗
[Exploration on statistical method for calculating intensity of the infection in residents in nationwide sampling survey of schistosomiasis].
AIM: To set up statistical methods for calculation of intensity of schistosome infection in residents in nationwide sampling survey of schistosomiasis. METHODS: Based on the epidemiological survey of schistosomiasis and according to the medical statistics for multiple stratifying and clustering random sampling, the calculation method on intensity of schistosome infection was inferred. RESULTS AND CONCLUSION: Nine formulas were worked out for the calculation of the intensity of schistosome infection in different endemic provinces with different ecotypes. These formulas are of practical value.
China↗
[Bio-mechanical property changes of long bone in ovariectomized goats].
This paper presents the biomechanical property changes of long bone in goats at 180 days after ovariectomy. 10 female goats at the age of 1.5 +/- years(average body wight 22 kg) were randomly divided into the control group and ovariectomized group. These goats were sacrificed at 180 days after ovariectomy and the femora, tibiae, metatarsus, humeri, radius and metacarpus were taken out bilaterally. The structural mechanical properties of these bones were measured with the three-point bending method. The material mechanical properties of flexura, compress and tensile in the compact bone specimens taken from the two broken ends were also measured. The results showed that the bone density, bone thickness in broken ends, and the break load of three-point bending for the long bones in the OVX group were obviously lower than those in the control group (P < 0.05). And compared with the control group all the long bones in the OVX group, except the femur, had significantly lower ultimate bending strength, bending elastic modulus; ultimate flexural strength, flexural elastic modulus; ultimate compressing strength, and compressing elastic moduus. Some long bones in the OVX group had significantly decreased ultimate tensile strength (P < 0.05). These indicated that the mechanical properties of long bone in OVX group at 180 days after ovariectomy were reduced in varying degrees and were liable to fracture. This result is in accord with the clinical findings in osteoporosis resulting from post-menopausal estrogen fall and is also consistent with the osteoporotic model of ovariectomized rats. The authors conclude that the bone biomechanical properties are important parameters in assessing osteoporosis and the ovariectomized goat can possible be a good big animal model of osteoporosis.
Animals↗
[A biomechanical evaluation of the effects of XW630 on the healing of experimental fracture].
The aim of this study was to evaluate whether tetracycline-estrone (XW630) plays a role in the healing of fracture. 120 rabbits were divided into 4 groups and subjected to osteotomies at the middle of both radials, and they were given XW630, estrone (E2), tetracycline (T) and 0.9% sodium chloride (C) respectively after operation by means of intramuscular injection (M) or local injection(L) in the fracture region. The biomechanical properties, including bending and compression, and the biochemical changes were tested at 30 days, 60 days and 90 days after osteotomy. The results showed that the bending stress and compression stress in the XW630(M) group were significantly higher than those in other groups at different periods after osteotomy. At 30 days after osteotomy, the bending ultimate stress in the XW630(M) group was 80% higher than that in group C, 76% higher than that in group E2, and 69% higher than that in group T. At 90 days after osteotomy, the bending ultimate stress in XW630(M) group increased to 80% of that of the normal radius, and it was 30% higher than that of group C, 34% higher than that of group E2 and 19.5% higher than that of group T. The biochemical changes corresponded with the changes biomechanical properties. These data suggest that XW630 given by intramuscular injection plays an obvious role in accelerating the healing of fracture. The medicine given by intramuscular injection is obviously more effective than that locally injected.
Animals↗
Transmission and natural regulation of infection with Ascaris lumbricoides in a rural community in China.
A longitudinal study on a cohort of 215 people who remained untreated from June 1993 to June 1994 was carried out using fecal egg counts to examine the course of transmission and aspects of the natural regulation of the infection with Ascaris lumbricoides. The results indicate that the parasite population remained relatively stable during the years since overall prevalence remained steady above 60%. About 40% of the people in the cohort retained their infection intensity status throughout the year, in terms of light, medium, and heavy infections. The regulation of infection in the population could be attributed to the tendency for the loss of worms from people with initial light infections and the movement to medium infection intensity status of people with both light and heavy infections. Compared with adults, children showed a stronger tendency of retaining initial medium infections but weaker tendencies for keeping initial light infections and clearing themselves of infection. Predisposition to light and medium infections was detected in people who had experienced an uninfected period of 4-12 mo before reinfection was established.
Adolescent↗
A family-based analysis of the association of the dopamine D2 receptor (DRD2) with alcoholism.
The possible association of the DRD2 locus, and in particular the Taql-A1 allele, with alcoholism remains controversial, in part because of differences in allele frequencies among populations. To avoid problems associated with differences in allele frequencies in different populations, we tested whether the DRD2 locus is associated with alcohol dependence in a large family-based sample. Neither the transmission/disequilibrium test nor the Affected Family-Based Controls test provide any evidence of linkage or association between the DRD2 locus and alcohol dependence.
Alcoholism↗