Search PubMed⌕ Search

Biomedical subjects

W Witte

Publications and source records attributed to W Witte.

At least 127 records · Page 7Linked to original sources
PubMed1984

Occurrence of a nonplasmid-located determinant for gentamicin resistance in strains of Staphylococcus aureus.

The occurrence of resistance to gentamicin in strains of Staphylococcus aureus with different phage patterns in two hospitals is described. The data suggest transfer of gentamicin resistance between different strains. In the strains investigated the determinant for resistance to gentamicin (parallel resistance to kanamycin and tobramycin) is located on the chromosome. The transfer of this resistance determinant in a mixed culture of donor and recipient cells in vitro is mediated by transduction because it is dependent upon lysogenicity of the donor for a serogroup-B phage and because it can be inhibited by gamma-globulin. The transfer is always associated with lysogenization of the recipient cells by this phage.

Bacteriophage Typing↗
PubMed1983

[Staphylococcus epidermidis as a rare cause of sepsis].

Implanted synthetic materials have to be included into the differential diagnosis of sepsis. This paper reports on a case of sepsis caused by Staphylococcus epidermidis in connection with a Spitz-Holter shunt. The interval between the implantation and the recognition of the endoplastitis took 4 years. The attempt of an antibiotic sanitation had no results. The fitness for work could be restored by removal of the shunt and reimplantation.

Adult↗
PubMed1983

A new semidefined nutrient medium for bacterial susceptibility testing.

A new semidefined nutrient medium for testing of bacterial resistance against chemotherapeutics is described. Nutrients, poor in antagonists, vitamins and ions are balanced. Full effectiveness in view of sensitivity and expression of bacterial resistance mechanisms of all chemotherapeutics tested is guaranteed. The nutrient medium, known under the trade name "Kulturmedium L4 zur Resistenzbestimmung" is comparable with the new recommended international media for this purpose. Beyond it the nutrient medium is useful for genetical investigations and combined with a diagnostic system for selecting different bacteria contained especially resistance properties.

Amino Acids↗
PubMed1982

Multiple drug-resistant Staphylococcus aureus with resistance to oxytetracycline and minocycline, an example for the distribution of a multiresistant strain-clone.

Multiresistant S. aureus-strains with chromosomally localized determinant for resistance to oxytetracycline-minocycline (tmn) exhibit uniform characteristics, obviously they have been spread as a clone. These strains harbour two plasmids: one with MW of 18 MD for beta-lactamase and determinants for resistance to mercury and to cadmium and one with MW of 2 MD for resistance to chloramphenicol. Beside the tmn-determinant, also the determinants for resistance to macrolides and to gentamicin are not located on plasmids. The tmn-determinant is specific for defined hospital strains reacting with group-III phages; it was not found in other strains of different origin.

Anti-Bacterial Agents↗
PubMed1981

[Role of phage in the transfer of plasmids in mixed cultures of Staphylococcus aureus (author's transl)].

Lysogenicity with phages of serogroup B is a prerequisite to the transfer of drug resistance plasmids in mixed cultures of Staphylococcus aureus. This is demonstrated by experiments with transfer of the plasmids pII 147, pC 221, pT 127, pE 2222 to strain 8325-4 which was lysogenized for different phages of different serogroups (Table 2). The transfer is also possible, when the donor strain or the recipient strain are lysogenic for the phage mutant phi 11, M 28, which is able to lyse strain 8325-4 but unable to form phage heads (Kretschmer and Egan, 1975). The fact that the induction of prophage phi 11 and steps of phage-propagation are necessary for the transfer, is evident from negative results obtained by use of the rec--mutant of strain 8325 (RN 981) as donor and by use of strain 8325-4 lysogenic for the phage mutant phi 11, A 4 as donor. The phage mutant phi 11, A 4 is defective at an early step of phage propagation. These experiments are shown in Tables 3 and 4. The possibility of transfer using donor or recipient strains which are lysogenic for a phage-mutant but unable to form phage heads clearly shows that the transfer is not based on transduction. Incubation of non lysogenic donor and recipient cells in the culture supernatant of strain 8325-4 (phi 11, M 28) or in the lysate of strain 8325-4 obtained after infection with phage phi 11, M 28 also allows the transfer of plasmids. Obviously one of the components of serogroup B phages is essential for the transfer.

Lysogeny↗
PubMed1981

[Resistance development in human Staphylococcus aureus due to the use of oxytetracycline and tylosin in industrialized swine production units].

Staphylococcus aureus strains from human nasal carriers without vocational contact with pigs could be differentiated from the majority of strains obtained from pathologically affected pigs by determination of host-specific-variety, according to Meyer (1966) and Witte et al. (1977). Less unambiguous findings regarding site variability were recorded from strains in nasal mucosa of pig-handling personnel, clinically intact pigs on farms, and slaughtered pigs. Antibiotics, such as oxytetracycline and macrolides were applied to pigs but failed for all practical purposes to have any bearings on the development of Staphylococcus aureus resistance in human probands with no vocational contact with animals. Personnel of pig units, however, exhibited moderate resistance to oxytetracycline and macrolides of Staphylococcus aureus strains which were typical of man. Macrolide resistance was determined, in that context, by one single-resistance plasmid.

Animal Husbandry↗