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Biomedical subjects

W Williams

Publications and source records attributed to W Williams.

At least 127 records · Page 7Linked to original sources
PubMed1988

Human monoclonal antibodies to phenolic glycolipid-I derived from patients with leprosy, and production of specific anti-idiotypes.

Human monoclonal antibodies (mAb) were produced by hybridomas derived from fusion of the GM4672 lymphoblastoid cell line and peripheral blood mononuclear cells from leprosy patients. Hybridoma supernatants were screened for immunoglobulin (Ig) secretion, binding to Mycobacterium leprae, phenolic glycolipid-I (Phen GL-I), the unique M. leprae glycolipid and single-stranded(ss)DNA by ELISA. On the basis of direct-binding ELISAs, two IgMk mAb (PR4 and TH3) were selected for characterization. PR4 and TH3 bound to M. leprae, Phen GL-I and ssDNA; PR4 also bound to M. avium and M. kansasii and TH3 to M. kansasii. Inhibition assays demonstrated that these antibodies did not bind to the terminal disaccharide of Phen GL-I. In addition, both PR4 and TH3 bound to several autoantigens: ssDNA, double-stranded(ds)DNA and poly(ADP-ribose) but not RNA. PR4 and TH3 were used for preparation of rabbit anti-idiotype antisera. Inhibition studies demonstrated that the affinity purified rabbit anti-idiotype antisera were specific for their respective idiotype and that both Phen GL-I and ssDNA inhibited binding of idiotype to its anti-idiotype. PR4, but not TH3, was found to be similar but not identical to the 16/6 idiotype originally identified on a human monoclonal anti-DNA antibody derived from a patient with systemic lupus erythematosus (SLE).

Antibodies, Antinuclear↗
PubMed1987

The criminal justice/mental health system and the mentally retarded, mentally ill defendant.

The mentally retarded, mentally ill defendant calls attention to problems in the relationship between the legal and the mental health systems. This study looks at what happens to a group of retarded offenders found incompetent to stand trial. The study examines differences in court processing and final disposition between mentally retarded and nonretarded defendants found incompetent to stand trial. Virtually all subjects were diagnosed as being psychotic. The mentally ill, retarded defendants spend significantly more time in the hospital, more time in the hospital waiting to be returned to jail, and more total time incarcerated in the criminal justice/mental health system. At a final disposition hearing mentally ill, retarded defendants were significantly more likely to be rehospitalized and less likely to be released to the community. No mentally ill, retarded defendant in this study went to prison. The longer periods of incarceration may stem from an underlying lack of understanding about the ability of retarded defendants to achieve competency. Differences in court disposition may result from a court/mental health professional tendency to select dispositions which are thought to be more 'humane'.

Commitment of Persons with Psychiatric Disorders↗
PubMed1987

Measurement of anti-DNA antibodies: a reappraisal using five different methods.

One hundred and thirty coded sera, 60 from patients with systemic lupus erythematosus (SLE) and 70 from patients with other autoimmune rheumatic diseases were tested for deoxyribonucleic acid (DNA) binding activity by five different types of assay. These were enzyme linked immunosorbent assay (ELISA) (distinguishing IgG and IgM anti-ssDNA and anti-dsDNA), Crithidia luciliae, a nitrocellulose filter assay, the Amersham kit, and another modified Farr assay, the radioimmunoassay (RIA) (UK). The Crithidia test was the most specific, none of the controls was positive, but the least sensitive (13% positive only). The RIA (UK) was the most sensitive (57% positive). In most of the assays 3-9% of the controls were positive. When the SLE sera were analysed according to disease activity the IgG anti-dsDNA ELISA, all three RIA values, and the Crithidia test values were raised in all the patients with severely active disease. Some patients with inactive disease, however, were positive in each of the tests. The best interassay correlations (r less than 0.49) were found between RIA (UK), and ss IgG and the Amersham kit; and between ds IgG and ss IgG. In the main, however, it was clear that different assays are dependent upon distinctive properties of DNA antibodies. It seems inevitable that most major rheumatology units will require more than one anti-DNA antibody assay.

Adolescent↗
PubMed1985

Long-term surveillance for adverse effects of antihypertensive drugs.

The large number of participants (5,485 patients) in the Hypertension Detection and Follow-Up Program, Stepped Care, form the largest group to date on which detailed surveillance of long-term antihypertensive therapy and drug side effects has been reported. Over a five-year period, among all hypertensive persons (mild, moderate, and severe combined) who were not taking antihypertensive medications at the beginning of the study and who attended the clinic at least once during the five-year trial, a total of 9.3% had definite or probable side effects severe enough to cause discontinuation of the drug treatment in question. An additional 23.4% had drug treatment discontinued due to possible side effects. Within the mild, moderate, and severe hypertension categories, 8.6%, 11%, and 12%, respectively, had definite or probable side effects. The incidence of side effects declined over the five years in all race-sex groups. Five-year incidence of total side effects was 29.8% in those aged 60 to 69 years at entry and 38.0%, 36.8%, and 34.1%, respectively, in those aged 50 to 59, 40 to 49, and 30 to 39 years. Sexually related side effects required discontinuation of treatment in 8.3% of male participants. However, less than 1% of active participants required hospitalization for side effects. No death that could be attributed to side effects was detected. Thus, the Hypertension Detection and Follow-up Program data, which have previously demonstrated the beneficial effects of antihypertensive therapy, confirm the relative safety of such therapy.

Adult↗
PubMed1985

Anaesthetic ejaculation.

Literature on the uncommon symptom of anaesthetic ejaculation and the relevant physiology is reviewed. Seven case vignettes describing the clinical pictures, background histories and common features of this problem are presented, along with some psychometric data. No physical causative factors could be elucidated. The main features shared in common by these men were problems in the recognition and expression of emotions, and a performance orientation. Treatment by sex-therapy procedures was ineffective.

Adult↗
PubMed1985

Isolated systolic hypertension in 14 communities.

In the Hypertension Detection and Follow-up Program, 158,906 individuals from 14 communities around the United States had their blood pressure measured in their homes in 1972-1973. Of the total population screened, 2.4% had isolated systolic hypertension (systolic blood pressure greater than or equal to 160 mmHg and diastolic blood pressure less than 90 mmHg). Isolated systolic hypertension was present for 0.5% of those aged 30-39 years and 6.8% among those aged 60-69 years. The prevalence in blacks and women was greater than the prevalences in both whites and men. The prevalence among those taking antihypertensive medications at the time of screening was 6.1%, and 1.9% among those not on antihypertensive medications. From the individuals with "normal" diastolic blood pressure on the single home measurement (less than 90 mmHg), a random sample of 5,032 individuals were followed for mortality for eight years. Prevalence of isolated systolic hypertension was similar in this sample to that in the total. Among those not on antihypertensive medications, 8-year life table all-cause mortality rates adjusted for age, race, and sex were 17.6% for those with systolic blood pressure greater than or equal to 160 mmHg and 7.7% for those with systolic blood pressure greater than 160 mmHg. Among this population, all of whom had a diastolic blood pressure less than 90 mmHg, a multiple logistic analysis adjusting for baseline treatment status, age, race, sex, education, smoking, weight, pulse, physical activity, and systolic blood pressure revealed that each millimeter increase in systolic blood pressure was associated with approximately a 1% increase in mortality over the eight years of follow-up (p less than 0.05). Isolated systolic hypertension is both relatively common and a significant risk factor for subsequent mortality.

Actuarial Analysis↗
PubMed1985

Detection and treatment of hypertension in older individuals.

The Hypertension Detection and Follow-up Program screened 34,012 individuals aged 60-69 years old in their homes in 14 communities around the United States during 1972 and 1973. The prevalence of hypertension, defined as diastolic blood pressure greater than or equal to 90 mmHg or on antihypertensive medication, was 42.1%. After a second clinic screen, 2,376 hypertensives were identified and randomized into Stepped Care, a special intensive treatment group, or Referred Care, a group referred to their usual medical care sources. These individuals were followed for five years (until they reached the ages of 65-74 years). Over the five years, 79.4% of older individuals remained under active care and 81.4% of those were at their goal diastolic blood pressure. Side-effects tended to be less frequent in older individuals than in younger ones. Older Stepped Care participants with mild hypertension (diastolic blood pressure 90-104 mmHg) had a 17.2% reduction in all-cause mortality over five years compared to Referred Care, which was primarily due to a reduction in deaths attributed to cardiovascular causes. Thus, antihypertensive treatment can be safely and beneficially administered to individuals in this age range with diastolic hypertension, including those with mild hypertension.

Adult↗
PubMed1985

Psychogenic erectile impotence--a useful or a misleading concept?

Psychological factors alone may not fully explain some cases of apparently purely psychogenic acute situational impotence. Chronic impotence is often due to a combination of aetiological factors. Erectile selectivity is not a reliable means of diagnosing purely psychogenic impotence. Nocturnal penile tumescence monitoring is not absolutely reliable in making a distinction between organic and psychogenic impotence. The either/or concept of impotence being organic or psychogenic should be abandoned as serious errors can be made by adhering to it.

Adult↗
PubMed1985

Monoclonal antibody to myosin derived from mice immunized with gastric mucosal cells.

A monoclonal IgMk antibody secreted by a hybrid (MUI-6) of mouse plasmacytoma NS-1 with spleen cells from a mouse immunized with canine parietal cell-enriched gastric mucosal cells was tested for immunofluorescence reactivity with gastric mucosal cells, tissue sections and monolayer cultures of rat fibroblasts. The antibody did not react with the cell membrane of parietal cells but reacted with smooth muscle fibres and skeletal muscle striations. In non-muscle cells, the antibody reacted with parietal cell cytoplasm, liver in a "polygonal" pattern, renal glomeruli, brush borders and peritubular fibrils of renal tubules, thymic medulla, brush borders of small intestinal mucosal cells, and cerebellar astrocytes, synaptic endings and synaptic glomeruli. In fibroblast monolayers, the antibody stained stress fibres in an interrupted pattern and in spreading fibroblasts, the antibody stained the microfibrillar network. Stress fibre staining was disrupted by treatment of cells with cytochalasin B. Immunoblots showed that the antibody reacted with a 200 K protein in 3T3 cells and with a preparation of myosin from rat liver.

Animals↗
PubMed1984

Secondary premature ejaculation.

Secondary premature ejaculation is a definite entity which can be due to a variety of causes--physical, pharmacological and psychological. The sparse literature on this subject is reviewed and a series of 25 cases of diverse and sometimes unusual aetiology reported. The fact that ejaculatory control can be adversely affected by a variety of physical and psychological conditions has implications for theories as to the cause of the much commoner entity of primary premature ejaculation, for which there is still no generally agreed-upon aetiological formulation.

Adult↗