Search PubMed⌕ Search

Biomedical subjects

W Wiegrebe

Publications and source records attributed to W Wiegrebe.

At least 55 records · Page 3Linked to original sources
PubMed1976

Determination of disulfiram and its metabolites in human blood.

This work was initiated by the lack of a sensitive method for the determination of disulfiram and its metabolites in blood of patients treated with this drug. A method is described which allows the separate determination of carbon disulfide, free diethyldithiocarbamate and disulfides derived from disulfiram with adequate precision in 10 ml patient blood. It is based on a spectrophotometric determination of a yellow compound formed by trapping carbon disulfide produced from diethyldithiocarbamate and disulfiram in an ethanolic solution of diethylamine and copper(II)-acetate. Good quantitation of disulfiram and diethyldithiocarbamate in blood was achieved by trapping carbon disulfide produced when formic acid and cystein were added to the samples. During daily administration of 200 mg disulfiram to humans, concentrations of zero to 0.6 mug carbon disulfide and 0.2 to 1.0 mug diethyldithiocarbamate per ml blood were found using this method.

Carbon Disulfide↗
PubMed1975

[Polarographic determination of some phenylsubstituted anticonvulsants].

A method is described to determine the anticonvulsive drugs phenobarbital, phenytoin, mephenytoin, methylphenobarbital and primidone. A serum extract is nitrated, separated by thin-layer chromatography, and the nitro compounds are determined by polarography. The rates of recovery are: phenobarbital 81%, phenytoin 79%, mephenytoin 80%, methylphenobarbital 86% and primidone 84%. The sensitivity limits range from 3.5 mug/ml plasma for phenytoin to 5.5 mug/ml for mephenytoin and methylphenobarbital. This method might be useful not only for determination of therapeutic blood levels but also for toxicology because it saves time and the expenditure of work is small. The mean deviation for mononitrated anticonvulsive drugs is +/- 2.8% (for 50 mug/ml) and +/- 11.6% (for 10 mug/ml). For phenytoin being dinitrated, the values are +/- 0.6% and +/- 7.5% for 50 mug and 5 mug, respectively). We determined blood levels of out-patients containing phenobarbital and phenytoin besides other substances. Having once established calibrating curvers internal or external standards are not required.

Anticonvulsants↗