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Biomedical subjects

W Weidner

Publications and source records attributed to W Weidner.

At least 235 records · Page 13Linked to original sources
PubMed1983

Chlamydia trachomatis in 'abacterial' prostatitis: microbiological, cytological and serological studies.

Chlamydia trachomatis was isolated from urethral swabs after prostatic massage in patients with 'abacterial' prostatitis. After exclusion of prostatic infections by gram-negative bacteria, enterococci and ureaplasmas, in 20 of 43 patients with apparent monoinfection by C. trachomatis, a leucocytal reaction was demonstrated in prostatic secretions. By microimmunofluorescence test, antibody titers greater than or equal to 1:8 against various types of C. trachomatis were detected in these patients, whereas serology was negative in chlamydia-positive patients without increased leucocyte counts (prostatodynia).

Adolescent↗
PubMed1983

[Enzyme-linked immunosorbent assay (ELISA) for IgG- and IgM antibodies in chlamydial infections of humans].

The development and evaluation of an indirect ELISA for IgG and IgM antibodies in human sera against the genus-specific chlamydial antigen is described. Application of commercial anti-immunoglobulins for production of conjugates usually resulted in direct conjugate/antigen reactions. Therefore, all immunoreagents had to be prepared by the authors. Using anti-immunoglobulins purified by affinity chromatography for the production of anti-human IgG(H + L)-peroxidase conjugates, these conjugates showed high specific activity and could be diluted 1/10,000 for the test, whereby direct reactions with antigens were negligible (12 +/- 12 ELISA units). Results with the mu-chain-specific IgM ELISA could be verified additionally by rheumatoid factor analysis and gel-chromatographic investigations. Reactions with sodium deoxycholate extracted antigen from cells not infected with chlamydiae ("negative antigen") were observed with sera from persons working with cell cultures. In comparison with microimmunofluorescence (MIF) test and radioimmunoassay (RIA), the ELISA was the most sensitive method. However, for differentiation of urogenital infections associated with chlamydiae, the type-specific MIF test was superior to the genus-specific ELISA. In comparison to persons with chlamydia-associated prostatitis, the high percentage of IgM-positive reactions in patients with nongonococcal urethritis (10% and 56%, respectively) pointed to a predominant participation of genus-specific IgM antibodies in recent infections. As compared to ELISA values of patients with infections of the urogenital tract (up to 875 ELISA units), in some apparently healthy blood donors significantly higher ELISA values (up to 1400 ELISA units) were recorded. Such reactions may be attributed to systemic infections, e.g. chlamydial pneumonias. The importance to differentiate between IgG and IgM antibodies in such infections is discussed.

Antibodies, Bacterial↗
PubMed1983

Significance of Chlamydia trachomatis in "abacterial" prostatitis.

Chlamydia (C.) trachomatis was isolated in McCoy cell cultures from urethral swabs after prostatic massage, of 43 out of 233 patients (18.5%) with symptoms of "abacterial" prostatitis, but also from 5 out of 65 control persons (7.7%). Numbers of granulocytes in sediments of cytocentrifuged urine voided after prostatic massage were normal (less than or equal to 2, magn. 400 X) in all 65 control persons, but were increased in 26 out of 43 patients with symptoms of "abacterial" prostatitis (greater than or equal to 4, magn. 400 X). Using an (H + L) chain specific anti-IgG FITC conjugate, microimmunofluorescence tests for detection of antibodies against C. trachomatis could be performed with the sera of all 65 control persons and with those of 37 out of the 43 patients. All control persons, even those five with positive C. trachomatis culture, were serologically negative (titer less than 1:8), while in 13 out of 15 C. trachomatis positive patients with a definitive diagnosis of "abacterial" prostatitis, humoral antibodies with titers of greater than or equal to 1:8, predominantly against serotypes I, J, E, and G, were detected. Serological results correlated well with granulocyte counts in urines after prostatic massage. Patients with symptoms of "abacterial" prostatitis with normal granulocyte counts (less than or equal to 2) and negative serology (titer less than 1:8) were considered suffering from "prostatodynia".

Adolescent↗
PubMed1983

Rheumatoid factor-negative arthritis, especially ankylosing spondylitis, and infections of the male urogenital tract.

146 men with rheumatoid factor-negative (sero-negative) arthritis, i.e., 97 patients with ankylosing spondylitis, 36 patients with Reiter's syndrome, and 13 patients with reactive arthritis, were examined for infections of the urogenital tract by following recently established criteria. 74 patients (50.7%) had infections of the male adnexes: 3 patients suffered from balanitis, 14 patients from urethritis, 49 patients from prostatitis, 1 patient from epididymitis, and 7 patients from urinary tract infection. Balanitis and urethritis were almost exclusively associated with Reiter's syndrome. In 37 of 97 patients with ankylosing spondylitis, a urogenital tract infection, mainly a prostatitis (31 patients), was detected. The microorganisms isolated most frequently from patients suffering from urethritis and prostatitis, were Chlamydia trachomatis and Ureaplasma urealyticum.

Arthritis↗
PubMed1982

[Studies on the aetiology of non-gonococcal urethritis (author's transl)].

Tests performed on urethral secretion and morning urine specimen identified the causative micro-organism in 143 of 164 males with non-gonococcal urethritis. In 59 patients (36%) there was an infection with Chlamydia trachomatis, in 48 (29.2%) with Ureaplasma urealyticum in significantly high bacterial counts (greater than or equal to 10(4) KbU/ml) urethral secretion (greater than or equal to 10(3) KbU/ml first urine sample), and in a further 18 patients (11%) double infections with Chlamydia trachomatis and Ureaplasma urealyticum. Other causative organisms such as Mycoplasma hominis, enterococci, streptococci of groups A and B, enterobacteria and Trichomonas were isolated in 18 patients (11%). In 21 patients (12.8%) no causative organism was demonstrated. In several instances, Chlamydia was demonstrated in ureaplasma-negative non-gonococcal urethritis, while Ureaplasma was demonstrated in Chlamydia-negative cases. In cases of urethritis caused by Chlamydia gonorrhoeal urethritis was more frequent in the past history than when urethritis was due to Ureaplasma. Treatment with tetracyclines led to clinically and microbiologically good results in 77% of patients with Ureaplasma urethritis, in 64% of patients with Chlamydia urethritis. Adnexial infections such as prostatitis and epididymitis were among the complications seen.

Adolescent↗
PubMed1982

Penile flow index and its role in classification of erectile dysfunction: a preliminary report.

A method is described to confirm vascular disorders in sexual impotence by a simple noninvasive screening technique. Doppler ultrasound signals are registered from superficial dorsal penile arteries and arteria radialis. The penile flow index (PFI) is determined by dividing the mean values of both measurements. An insufficient vascular support of the penis is assumed if the amplitude of the dorsal penile arteries is less than one third of that of the a. radialis. Identical Doppler signals are registered in superficial dorsal and deep cavernosal arteries.

Adolescent↗
PubMed1982

[Endocrine findings during hormonal treatment of metastasizing prostatic carcinoma].

Endocrine findings of hypophyseal, testicular and adrenal function in the hormonal treatment of advanced prostatic carcinoma are described. Diethylstilbestrol (DES) reduces significantly the plasma levels of testosterone, follicle-stimulating hormone (FSH) and luteinizing hormone (LH). In contrast, those of prolactin, dehydoepiandrosterone (DHEA), and cortisol as well as sexual hormone binding globulin (SHBG) are elevated. The additional administration of bromocriptine decreases prolactin to normal levels and further decreases the testosterone levels. The adrenal steroids are influenced differently: DHEA decreases as compared to the DES-treated group, while the cortisol levels rise significantly. The addition of cyproterone acetate to the therapeutic regimen decreases the cortisol levels, and all other values, including SHBG, are not altered significantly compared to the group treated with DES plus bromocriptine. What is of interest is that the estradiol levels are not altered by any treatment schedule. In a group of patients, orchiectomized prior to our study, the endocrine values are compared to those mentioned above. Following orchiectomy FSH and LH levels rise significantly, but not prolactin and SHBG. The testosterone levels were as low as under treatment with DES. From our results it is concluded that the combination of DES plus bromocriptine plus cyproterone acetate best achieves the inhibition of androgenic influence onto the prostatic carcinoma.

Androgens↗
PubMed1981

Experimental prostatitis in female Mastomys natalensis--a preliminary report.

Female Mastomys natalensis were inoculated with a suspension of Klebsiella pneumoniae into the bladder. All animals developed prostatitis without urinary tract infection between the 9th and the 12th day after inoculation. 10(4) - 10(5) CFU of K. pneumoniae per gram prostatic tissue could be cultivated. Histologically acute purulent prostatitis was detected in most cases 6 days after infection. In the follow-up periacinar infiltration predominated.

Animals↗
PubMed1981

[Thorotrast cancer following vesiculography in chronic prostatitis].

33 years after vesiculography using the X-ray contrast remedy Thorotrast on transurethral way in a patient with chronic prostatitis a carcinoma developed in the region of the vesical neck. Histologically the tumour imposed now as a differentiated urothelium carcinoma of medium degree. By autoradiography in the region of the tumour structures radioactive Thorotrast could be proved. On account of the close topographical relations and the long time of latency the development of the carcinoma seems to be ascertained by the then application of Thorotrast. The connections are discussed.

Aged↗
PubMed1981

[Behavior of phosphatases and carcino-embryonal antigens in serum following specific immunotherapy of metastasizing prostatic cancers].

The efficacy of specific immunotherapy was examined in patients suffering from metastatic prostatic cancer. For this purpose determination of prostatic acid phosphatase and carcino-embryonic antigen in serum was performed using conventional enzymatic and radioimmunological techniques before and after therapy. The results were as following: 1. A significant decrease of the above mentioned parameters was demonstrable after therapy. 2. The determination of prostatic acid phosphatase was helpful for immediate control of therapy, the determination of carcino-embryonic antigen was more helpful in follow-up studies. 3. Contrary to disappointing relevance of radioimmunological determined prostatic phosphatase in early prostatic cancer this parameter seemed to be more suitable in follow-up studies than conventional determined phosphatase.

Acid Phosphatase↗
PubMed1980

Quantitative culture of ureaplasma urealyticum in patients with chronic prostatitis or prostatosis.

We studied 187 men with symptoms and signs of chronic prostatitis or prostatosis and 108 healthy controls. Ureaplasma urealyticum was isolated from 103 (55.1 per cent) of the patients and from 24 (22.2 per cent) of the controls. When quantitative culture of Ureaplasma urealyticum is considered a typical histogram of prostatitis was evident in 36 patients (19.3 per cent) using the Stamey localization technique. In 20 of the 36 patients the etiology was uncertain for various reasons. Evidence was provided that Ureaplasma urealyticum could be considered as the etiologic agent of the disease in at least 16 (8.6 per cent) of the 187 patients with chronic prostatitis studied.

Adolescent↗
PubMed1980

[Reintervention after ureteroneocystostomy with special attention to replacement of ureter by an ileal loop (author's transl)].

After unsuccessful primary 'simple' ureteroneocystostomy using the methods of Frisch-Boeminghaus, Röhl-Ziegler, and Politano-Leadbetter, a second surgical intervention often can be performed after the methods of Boari and Küss. After unsuccessful primary 'combined' ureteroneocystostomy (Boari-Küss), a further ureter reimplantation mostly is not possible. In specially selected cases of unsuccessful ureterovesicoplasty we used an ileal loop as ureteral replacement. Our control period in 8 (14) patients is now up to 15 years. Long-term controls have shown in most patients a change to better renal function. The most important facts are the preoperative renal reserve and the original disease. After replacement of the ureter by an ileal loop a regular short-term control of urinary infection, blood urea and electrolyte balance is unavoidable.

Follow-Up Studies↗
PubMed1979

[Treatment of hospital-acquired urinary infections with amikacin (author's transl)].

The efficacy of amikacin was evaluated in 32 patients with urinary infections due to hospital-acquired rods. Treatment with amikacin was successful in all cases of acute urinary infection by gentamicin-resistent gram-negative strains. Amikacin shares with other aminoglycosides the risk of nephrotoxicity and ototoxicity. Follow-up studies are preferable to patients already suffering from damage of hearing. Lesions of n. vestibularis were relatively infrequent.

Amikacin↗