Biomedical subjects

W Wang

Publications and source records attributed to W Wang.

At least 361 records · Page 20Linked to original sources

PubMed2001

[A multicenter clinical study on clinical effects of meglumine cyclic adenylate in treating patients with chronic pulmonary heart disease].

OBJECTIVE: To observe the clinical effects of meglumine cyclic adenylate (MCA) in treating patients with chronic pulmonary heart disease. METHODS: 785 patients with chronic pulmonary heart disease were divided into two groups: MCA group and control group. Both groups of patients were treated with the same ordinary methods, but MCA was given in the MCA group. The period of treatment was 5-7 days. The degree of improvement in clinical symptoms, signs, ECG, pulmonary ventilation function and arterial blood gas analysis of the two groups before and after treatment were compared. RESULTS: The rates of improvement in clinical symptoms and signs were 92.2% and 73.6% in MCA group and control group respectively. VC increased (426 +/- 123) ml and (313 +/- 145) ml respectively as compared with the values before the treatment. FEV(1) increased (134 +/- 54) ml and (95 +/- 55) ml, PEF increased (0.78 +/- 0.32) L/s and (0.47 +/- 0.32) L/s and PaO(2) increased (23.4 +/- 9.5) mm Hg and (17.3 +/- 10.4) mm Hg respectively. The rates of ECG improvement were 63.7% and 41.2% respectively. There was significant difference between the two groups (P < 0.01 and 0.05). CONCLUSION: Significant therapeutic effects were obtained in treating patients of chronic pulmonary heart disease with respiratory and cardiac failure by using ordinary treatment measures combined with MCA injection.

Aged↗

PubMed2001

[A study on the bioavailability of fluoride added into milk].

OBJECTIVE: To assess the bioavailability of fluoride added into milk. METHODS: Eight subjects were studied by testing fluoride levels in blood and urine samples collected after drinking fluoridated milk and fluoridated water respectively, according to pharmacokinetics and cross control method. RESULTS: The bioavailability of fluoride in fluoridated milk reached 80.2% in blood analysis and 84.8% in urine analysis. The result was about 20.0% lower as compared with fluoridated water. CONCLUSION: Fluoride bioavailability in fluoridated milk is still high and sufficient.

Adult↗

PubMed2001

[Impact of substrate salinity on caloric value, energy accumulation and its distribution in various organs of Bruguiera gymnorrhiza seedlings].

The study with artificial culture showed that the accumulation of dry matter and energy in Bruguiera gymnorrhiza seedlings was promoted by low substrate salinity, but inhibited by high substrate salinity, which peaked at 10 mg.g-1 substrate salinity. With the increase of substrate salinity, the dry matter and the energy tended to be allocated to the micro-roots and leaves, which are nutrient-absorbing organ and photosynthetic organ, respectively. With the increase of substrate salinity, the changes in caloric values were differed in various organs of B. gymnorrhiza seedlings, and those in leaves could reflect the changes in the salt-resistance of the seedlings.

Caloric Tests↗

PubMed2001

[Poison effect of plant granules on Oncomelania hupensis].

Plant granules (phi 2-3 mm) made of starch, gelatin and medicine plant powder were used to kill Oncomelania hupensis. The results showed that after eating the plant granules, the mortality of the snail was 92.5%-100% within 24 hrs. The poison effect of the plant granules was much better than that of others, e.g., they could kill snails 12-24 h earlier than Niclosamidum (1 x 10(-3) g.L-1). In a field experiment, the snail mortality was up to 61.5% by using plant granules.

Animals↗

PubMed2001

Glasgow Coma Scale, brain electric activity mapping and Glasgow Outcome Scale after hyperbaric oxygen treatment of severe brain injury.

OBJECTIVE: To study the effect of hyperbaric oxy gen (HBO) treatment of severe brain injury. METHODS: Fifty-five patients were divided into a treatment group (n=35 receiving HBO therapy) and a control group (n=20 receiving dehydrating, cortical steroid and antibiotic therapy) to observe the alteration of clinic GCS (Glasgow Coma Scale), brain electric activity mapping (BEAM), prognosis and GOS (Glasgow Outcome Scale) before a nd after hyperbaric oxygen treatment. RESULTS: In the treatment group GCS, BEAM and GOS were improved obviously after 3 courses of treatment, GCS increased from 5.1 to 14.6 (P<0.01-0.001),the BEAM abnormal rate reduced from 94.3% to 38% (P<0.01-0.001), the GOS good-mild disability rate was 83.7%, and the middle-severe disability rate was 26.3% compared with the control group. There was a statistic significant difference between the two groups (P<0.01-0.001). CONCLUSIONS: Hyperbaric oxygen treatment could improve obviously GCS, BEAM and GOS of severe brain injury patients, and effectively reduce the mortality and morbidity. It is an effective method to treat severe brain injury.

Adult↗

PubMed2001

[An experimental study of antisense TGF-beta 1 inhibiting keloid fibroblast proliferation in vitro].

OBJECTIVE: To study the effect of antisense TGF-beta 1 on keloid fibroblast proliferation in vitro. METHODS: The antisense TGF-beta 1 was transfected into the keloid fibroblast by liposome. The cell count technique was used to test the effect of antisense TGF-beta 1 on keloid fibroblast proliferation in vitro. Cell apoptosis was tested by FCM. RESULTS: Antisense TGF-beta 1 can inhibit the proliferation of keloid fibroblast in vitro; Antisense TGF-beta 1 can increase the apoptosis ratio of keloid fibroblast in vitro. CONCLUSIONS: Antisense TGF-beta 1 can induce keloid fibroblast apoptosis and inhibit keloid fibroblast proliferation in vitro, which may contribute to the inhibition of keloid formation in vivo.

Antisense Elements (Genetics)↗

PubMed2001

[An experimental study of repairing nerve gap with acellular basal lamina tubes].

OBJECTIVE: To develop an effective new material for repair of nerve defects. METHODS: The 4-week pre-degenerative and normal sciatic nerve segments of SD rats were extracted with the detergent lysophosphatidylcholine, which resulted in acellular nerve basal lamina tubes, called EDNG (extracted degenerative nerve grafts) and ENG (extracted normal nerve grafts) respectively. The morphology of the acellular nerve basal lamina tubes was revealed by conventional histological examinations, transmission electron microscopy and immunohistochemistry method. The basal lamina tubes, as allografts, were used to repair a 15 mm-gap of sciatic nerve in Wistar rats. In the control group nerve autografts (NAG) was applied. Evaluation included general observation, electromyographic examination, muscle measurement and histological observation of serial sections at 12 weeks after surgery. RESULTS: The EDNG had more ideal frame, better resilience than ENG. There was no any cell debris in EDNG, whereas in the inner wall of the tubes immunohistochemical analysis revealed clear positive staining and strong immunoreactivity of laminin under immunoelectron microscopy. After peripheral nerves were repaired, all the animals of the three groups had restoration of function to a certain extent. The result of the NAG group was the best, the EDNG group was the next and the ENG group was the inferior. CONCLUSION: This new material offers a possible solution to repair of a short defect of a peripheral nerve.

Animals↗

PubMed2001

[Phacoemulsification for short eye axis cataract].

OBJECTIVE: To analyze the operative difficulty of cataract phacoemulsification in an eye with shorter axial length and discuss its preventive measures. METHODS: A retrospective study was performed to analyze the 122 patients (143 eyes) who had undergone cataract phacomulsification. Their ocular axial lengths were 20.21 to 22.10 mm. RESULTS: Because these patients had shorter ocular axis, shallower anterior chamber depth, narrower space in the anterior ocular segment and higher vitreous pressure, the operation was hard to perform. The rate of posterior capsular rupture was 7.69%. The postoperative inflammatory reaction was severe. The rate of reversible corneal edema (29.37%) was obviously higher than that in cataractous eyes with normal ocular axis (6.7%, P < 0.01), and also higher than that in eyes with normal ocular axis and hard nucleus (12.5%, P < 0.05). CONCLUSIONS: It is emphasized that the correct technique of incision, application of chopping and breaking nucleus technique, lower energy and higher aspiration for effective phacoemulsification with the help of Viscoat viscoelastic material and maintenance of deep anterior chamber are the key procedures to obtain satisfactory operative results.

Adult↗

PubMed2001

[Study of triglyceride distribution aged 35 - 64, and their association with other cardiovascular disease risk factors in 11 provinces].

OBJECTIVE: Study of triglyceride distribution and their association with other major cardiovascular disease risk factors in 11 provinces. METHODS: Twenty-seven thousand and sixteen persons in 11 provinces, aged 35 - 64, were screened for cardiovascular disease risk factors. RESULTS: (1) Results of plasma triglyceride level distribution showed that there was significant difference among 11 province, the highest/lowest ratio was 2.3. (2) Plasma triglyceride level was higher in men than in women (P < 0.001). (3) The prevelance of hypertriglyceridemia was the most frequent type of the dislipidemia. (4) Multiple stepwise regression analysis showed that total cholesterol, high-density lipoprotein cholesterol, body mass index, blood pressure, blood glucose and physical activity, significantly and independently correlated with hypertriglyceridemia prevelance. (5) There existed aggregation of risk factors in hypertriglyceridemia. Seventy-one point zero percent of hypertriglyceridemia had at least one other risk factors in men and 74.4% in women, while it was 53.8% men and 52.2% women of those with non hypertriglyceridemia (P < 0.01). CONCLUSION: Our date indicated that fasting triglycerides were associated with more arteriosclerosis factors and could serve as a marker for several cardiovascular disease risk factors. More patients with hypertriglyceridemia combined with other situation of metabolic syndromes thus might have higher risks.

Adult↗

PubMed2001

[A study on the epidemiological characteristics of obesity in Chinese Adults].

OBJECTIVE: To elucidate the epidemiological characteristics of obesity in Chinese adults and to provide scientific basis for public health policy-making, as well as for prevention and control of obesity among Chinese. METHODS: Using the data from diabetes mellitus, epidemiological studies carried out in 11 provinces/autonomous regions/municipalities of China from July 1995 to June 1997, including 42 751 subjects aged 20 - 74 years who had lived in the communities for no less than 5 years. Diagnostic criteria was based on the recommendation from WHO. Epidemiological characteristics of overweight and obesity were analysed. RESULTS: The prevalence rate of overweight and obesity among Chinese adults were 21.51% and 2.92% respectively. The age-standardized prevalence rates of overweight and obesity standardized by Chinese population aged 20 - 74 years were 18.28% and 2.48% respectively in 1990, when standardized by the standard population of world in 1992, the prevalence rates were 18.61% and 2.49% respectively. The prevalence of overweight and obesity in female (21.71% and 3.73% respectively) were significantly higher in male (21.25% and 2.11% respectively), the prevalence rates of obesity in male and in female increased with the increase of age, with the lowest prevalence in 20 year old group (1.23% and 0.96% respectively), the highest were 60 year old group (3.30% and 6.91% respectively). Among 11 provinces, the highest prevalence rates of overweight and obesity were in Beijing (34.75% and 5.14% respectively) of males, and in Shandong (34.56% and 9.36% respectively) of females. The prevalence rates of obesity were significantly higher in northern part of the country (2.64% in males and 4.85% in females) then in the south (1.36% in males and 2.15% in females), as well as significantly higher in urban (2.65% in male and 4.21% in female) than in rural (0.88% in male and 2.80% in female). CONCLUSION: Overweight and obesity had become prevalent in Chinese adults, more seen in the north than in the south, more in females than in males, and in older age than in younger, suggesting an earlier research and practice of prevention and control for overweight and obesity need to be implemented in China.

Adult↗

PubMed2001

[Assessment of quality of life in patients with diabetes mellitus and impaired glucose tolerance].

OBJECTIVES: To understand the quality of life (QOL) in patients with diabetes mellitus and impaired glucose tolerance, and investigate factors affecting their QOL. METHODS: Using the SF-36 instrument to assess QOL among 108 patients with diabetes mellitus, 109 patients with impaired glucose tolerance and 116 normal glucose tolerance subjects. RESULTS: In subjects with diabetes mellitus, the proportion of general perceived health assessed to be excellent or good was 12.04%; as compared with the same-age people, the proportion assessed to be good or fairly good was 62.04%. In subjects with impaired glucose tolerance, the two proportions was 13.76% and 69.72% respectively. The total score attained excellent or good level was 72.23% and 83.49% respectively in patients with diabetes mellitus and impaired glucose tolerance. The mean score of multi-item dimensions assessment (ranged from 58.33 to 87.38) decreased in diabetes mellitus; the lowest score being emotional role functioning and the highest mean score being physical functioning. Compared with normal glucose tolerance subjects, the mean score of physical functioning, physical role functioning, general health perception, vitality and the total score in diabetes mellitus was significantly decreased; and compared with in impaired glucose tolerance patients, the mean score of vitality, mental health and the total score was significantly decreased in diabetes mellitus. The result of correlation analysis demonstrated that age, occupational, duration of disease, number of symptoms and complications, level of fasting blood glucose and 2-hour past oral blood glucose affected the QOL of diabetes mellitus patients. CONCLUSIONS: The results suggested that to enhance the QOL in patients with diabetes mellitus, control of plasma glucose, pay attention to psychological treatment and measures related to diabetes mellitus with different characteristics must be early adopted.

Adult↗

PubMed2001

[Preparation of genetically engineered retinal pigment epithelial cells capable of secreting nerve growth factor].

OBJECTIVE: To develop genetically engineered retinal pigment epithelial (RPE) cells which are able to express bioactive nerve growth factor (NGF) steadily and efficiently and to combine gene therapy with retinal transplantation techniques to treat retinal degeneration. METHODS: Replication-deficient retrovirus-plasmid recombinant pLXSN-NGF was constructed via subclone and clone, and then packaged into pT67 cells to obtain virion containing a secretable form of NGF gene. We evaluated titer of the retroviral recombinant with NIH 3T3 cells and then transduced NGF gene into cultured RPE cells by the retroviral recombinant. We also analyzed the expression of NGF gene in transduced and untransduced RPE cells via reverse transcription (RT)-PCR and Western blot. The bioactivity of expressed NGF were evaluated by stimulating PC 12 cells with conditioned medium from transduced and untransduced RPE cells. RESULTS: The titer of replication-deficient retroviral recombinant reached 1.2 x 10(7) cfu/ml. NGF protein and mRNA were detected in the conditioned medium from transduced RPE cells, but not from the normal RPE cells. Marked neurite process outgrowth from PC 12 cells was observed after being stimulated with the conditioned medium from transduced RPE cells. CONCLUSIONS: RPE cells are able to be transduced by replication-deficient retrovirus with high efficiency, and secret bioactive NGF at a high level, which might be an ideal candidate for engineered cells.

3T3 Cells↗

PubMed2001

[Overexpression of bcl-2 protects HCC-9204 hepatoma cells from ethanol-induced apoptosis].

OBJECTIVE: To explore the effect of apoptosis-associated gene bcl-2 overexpression on ethanol-induced apoptosis in hepatocellular carcinoma (HCC) cells. METHODS: The plasmid of retrovirus expression vector pDOR-SB, which contains human bcl-2 cDNA, was transfected into a HCC cell line HCC-9204 cells whose bcl-2 protein expression is below detectable level. The expression of bcl-2 protein was detected by immunohistochemical ABC method. The cells were cloned continually until a monoclonal cell strain which expressed bcl-2 protein at a 100% positive rate was obtained, and the cells were detected with flow cytometry. After the above cells were treated with 6% ethanol for 6 h, the happening of apoptosis were detected by MTT assay, TUNEL assay and flow cytometry. RESULTS: bcl-2 protein expression was detected in most of the cells which had been transfected with pDOR-SB, while it was bcl-2 protein negative in cells transfected with pDOR empty vector or non-transfected HCC-9204 cells. The obtained monoclonal cell strain expressed bcl-2 protein at a 100% positive rate under flow cytometer after being cloned 3 times in succession, and was named HCC-bcl-2. After being treated with 6% ethanol, the optical absorbance data of HCC-bcl-2 cells and non-transfected HCC-9204 cells detected by MTT assay were 0.519 +/- 0.053 and 0.366 +/- 0.046, respectively, the former was significantly higher than the latter (P < 0.01). Their TUNEL index was 0.387 and 0.613 respectively, and their sub-G1 apoptotic peak scale was 3.8% and 10.7% respectively, the former were all lower than the latter (P < 0.05). CONCLUSION: Overexpression of bcl-2 protein can suppress ethanol-induced apoptosis in HCC-9204 hepatoma cells.

Apoptosis↗

PubMed2001

[Micro -- ecological investigation of burn wound infection].

OBJECTIVE: To analyze the epidemic tendency of the bacteria causing burn wound infection, and to explore the influencing factors. METHODS: Bacterial ecology on burn wound was investigated in 210 cases of burn patients admitted to our hospital from January 1999 to December 2000, and the results were compared to those done in the 1970s, 1980s and 1990s. RESULTS: Since 1999, G(+)cocci increased obviously (52.17%) when compared with those of the past three decades (P < 0.01), while G(minus sign) bacilli decreased significantly (46.48%, P < 0.01). The ratio of Pseudomonas aeruginosa in recent two years was evidently lower than those in the early 1970s and 1990s (P < 0.01) while the ratio of staphylococcus aureus was much higher than those in the middle of 1980s and 1990s (P < 0.01), in which methacillin -- resistant Staphylococcus aureus (MRSA) accounted for 85% or so, which increased significantly than those in the middle of 1980s and the beginning of 1990s (P < 0.01) with an increasing tendency. CONCLUSION: The increasing tendency of Staphylococcus aureus in our unit for the recent two years might closely be related to the preferential application of Imipenem in burn infection control. Furthermore, the ratio of MRSA in Staphylococcus aureus could not be lowered by vancomycin.

Anti-Bacterial Agents↗

PubMed2001

[Apoptosis of bone marrow cells aplastic anemia patients treated with cyclosporin A].

OBJECTIVE: To evaluate the effects of cyclosporin A (CsA) on the apoptosis of bone marrow cells of aplastic anemia (AA) patients. METHODS: The apoptosis of and Fas antigen expression on the bone marrow mononuclear cells (MNC) in 25 AA patients and 10 normal controls were assayed by TUNEL and FACS, respectively. RESULT: The percentage of CD(34)(+) cells was decreased significantly in AA patients than in normal controls (P < 0.05), and so did the percentage of CD(34)(+)Fas(+) cells (P < 0.05). The percentage of apoptotic cells in AA patients was higher than that of controls (P < 0.01). After the patients were treated with CsA for 2 months, the percentage of apoptotic cells was decreased (P < 0.01). The percentage of CD(34)(+)Fas(+) cells was positively correlated with that of apoptotic cells in AA patients. CONCLUSION: Fas is involved in the apoptosis of CD(34)(+) cells of AA patients. CsA could reduce the percentage of apoptosis of bone marrow cells in AA patients.

Adolescent↗

PubMed2001

[Study on the relationship between apoptosis and reactive oxygen species of cancer cell lines induced by anticarcinogens].

OBJECTIVE: To investigate the relationship between apoptosis (AP) and fluorescent intensity of reactive oxygen species (ROS) in cancer cell lines induced by anticancer agents, and the mechanisms of drug sensitivity and resistance. METHOD: K562 cells were treated respectively with each of the Vp16, ADR and DDP, the fluorescent intensity of ROS and AP percentage of K562 cells at different concentration of the drugs treated were assayed by flow cytometry (FCM). RESULT: (1) After treatment with these drugs for 24 hours, the fluorescent intensity of ROS and AP percentage of K562 cells increased obviously as compared with that of control groups. (2) The optimum concentrations of the drugs for ROS production and AP of K562 cells were determined. CONCLUSION: Anticancer agents induced AP of K562 cells, which might be through stimulating ROS production.

Antineoplastic Agents↗

PubMed2001

[Effects of Rholida on the free radical metabolism and serum creatine kinase after exercise at plateau].

Objective. To study the effect of Rholida on free radical metabolism and serum creatine kinase CK) after exercise at plateau. Method. After staying at high altitude (4100 m) for 20 d, 40 healthy young men were divided into 4 groups randomly (Rholida, Acetazolamide, Xi' s capsule and control, 10 men each group). And their SOD, MDA, GSH-Px CK, and CK-MB were determined respectively. Before, after taking drugs and after finishing the 5 min-stair-exercise. Result. Before taking drugs and after exercise, MAD GSH-Px, CK, CK-MB, increased as compared with quiet state (P<0.05, P<0.01), but SOD showed no significant chang (P>0.05). After taking drugs for 6 d, those who took Rholida, Acetazolamide and Xi's capsule, their MAD, GSH-Px CK, CK-MB increased after exercise as compared with quiet state (P<0.05). In Rholida group SOD increased and had significant change (P<0.05); but there was no significant change in Acetazolamide, Xi' s capsule group, SOD increased, MDA decreased (P<0.05), CK, CK-MB had no significant change (P>0.05), GSH-Px increased in Xi's group (P<0.05), but not in Acetazolamide group (P>0.05). SOD, GSH-Px increased, MDA, CK-MB decreased in Rholida group after taking drugs and the changes were significant (P<0.01). In Acetazolamide and Xi's capsule group, GSH-Px increased significantly, MDA, CK, CK-MB decreased significantly (P<0.05), but SOD didn't (P>0.05). Conclusion. Rholida, Acetazolamide, Xi's capsule could regulate the disorder of free radical metabolism at plateau and Rholida had advantage over the others.

Acetazolamide↗

PubMed2001

[Research on the transformation of ginsenoside Rg1 by intestinal flora].

OBJECTIVE: The decomposition of Ginsenoside Rg1(Rg1) by intestinal bacteria in rats or human was investigated both in vitro and in vivo. METHOD: The decompositions were investigated by means of thin-layer chromatography and Electron spurt ion mass. RESULT: It was found that Rg1 was converted into two metabolites [Rh1 and 20(S)-protopanaxatriol(20S-Ppt)] by human intestinal bacteria in vitro, while three compounds [Rh1, F1, and 20(S)-Ppt] were detected in rat intestinal bacteria metabolism in vitro and in vivo. CONCLUSION: The decomposition and/or metabolism of Rg1 in rat and human digestive tract was confirmed. The mode of metabolism in rat is Rg1-->Rh1(F1)-->Ppt, while in human it is Rg1-->Rh1-->Ppt.

Animals↗